Labshake search
Citations for Qiagen :
401 - 450 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... which included two separate DNA digestions (on-column then off-column) with DNAse (Qiagen), all according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... An on-column digestion step using the RNAse-free DNAse Set (Qiagen, Cat.No# 79254) was included for all RNA-Seq samples ...
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bioRxiv - Plant Biology 2020Quote: ... DNA digestion was performed using the RNase-Free DNase Set (Cat No.: 79254, Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... including an on-column DNA digestion using the RNAse-free DNAse set (Qiagen; 79254), was used to purify RNA from whole organoids ...
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bioRxiv - Neuroscience 2022Quote: ... An on-column DNase digestion was performed with the RNase-free DNase set (Qiagen). RNA was quantified with the Nanodrop 2000c (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: ... Contaminating genomic DNA was removed by digestion with RNase-Free DNase Set (QIAGEN, Germany). Library preparation and data analysis were performed as described previously (Shih et al. ...
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bioRxiv - Genetics 2022Quote: ... according to the manufacturer’s protocol with the step of on-column DNA digestion (QIAGEN) to remove genomic DNA ...
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bioRxiv - Neuroscience 2020Quote: ... according to manufacturer’s instructions and with 15min on-column DNase digestion (Qiagen, Venlo, Netherlands). cDNA was prepared with reverse transcriptase using iScript kit (BioRad Laboratories ...
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bioRxiv - Evolutionary Biology 2019Quote: ... On-column DNase digestion was performed using the RNase-Free DNase Set (Qiagen #79254).
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bioRxiv - Biophysics 2019Quote: ... column and protocol including on-column RNase-free DNase digestion (Qiagen; Cat No. 79254) and eluted into 30 μL RNase free water.
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bioRxiv - Molecular Biology 2020Quote: ... on-column digestion of DNA was additionally performed using RNase-Free DNase Set (QIAGEN) as a precautionary measure ...
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bioRxiv - Cancer Biology 2021Quote: ... a DNAse digestion step was included using the RNAse-free DNAse set (Qiagen #79254). Total RNA was isolated using the miRNeasy Mini Kit (Qiagen # 74004) ...
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bioRxiv - Immunology 2020Quote: ... Residual DNA was removed from the cleared lysate using DNase digestion on columns (Qiagen). mRNAs were eluted in 40μL of RNase-Free water ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... according to the manufacturer’s instructions including the on-column DNase digestion step (#79254, Qiagen). cDNA was synthesized using a high capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... according to the manual and including an on-column DNase I digestion (Qiagen, 79254). A second DNase digestion was performed on the eluates ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with the addition of on-column DNase I digestion (Qiagen RNase-Free DNase Set). We then added 2.5 μl SUPERase-InTM RNase inhibitor (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... including an on-column DNase digestion using the RNase-free DNase Set (79254; Qiagen).
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bioRxiv - Genomics 2021Quote: ... including an on-column DNase digestion using the RNase-free DNase Set (79254; Qiagen). cDNA was prepared from 0.5-1 μg total RNA with the QuantiTect reverse transcription kit (205311 ...
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bioRxiv - Cell Biology 2020Quote: ... with on-column DNase digestion with the RNase-Free DNase Set (79254, QIAGEN GmbH), both according to the manufacturer’s instructions (quick start protocol version ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was subjected to on-column DNA digestion with RNase-free DNase (Qiagen) and post-isolation DNA digestion using DNA-free DNase Treatment and Removal System from Ambion (Austin ...
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bioRxiv - Genetics 2022Quote: ... that included the on-column DNase digestion using RNase-Free DNase set (Qiagen, MD). KAPA Stranded mRNA-Seq kit (KAPA Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... per 1 ml buffer RLC and on-column Dnase digestion with Dnase I (Qiagen). First-strand cDNA was synthesized from 1 μg of total RNA using a Thermo Scientific RevertAid RT Kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... On-column DNase I digestion was conducted using RNase-free DNase I (Qiagen #79254). The concentration of extracted RNA was determined using a NanoRNA kit (Agilent #5067-1511 ...
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bioRxiv - Plant Biology 2022Quote: ... with off column DNase digestion using Qiagen’s RNase-Free DNase Set (Qiagen, Germantown, MD). Each sample was the pool of roots or leaves of two seedlings with three replications per tissue ...
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bioRxiv - Microbiology 2022Quote: ... After the SrfI digestion and gel purification of the large fragment of pcutFOG (Qiagen Gel purification kit ...
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bioRxiv - Cell Biology 2023Quote: ... and on-column DNAse digestion was performed to further purify the product (Qiagen, 79254). Isolated RNA was converted to cDNA using the Maxima First Strand cDNA Synthesis Kit for qRT-PCR as per manufacturer’s instructions (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... The DNase digestion was done using an RNase-free DNase set (Qiagen©, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... including an on-column DNase digestion using the RNAse-Free DNase set (Qiagen 79254). RNA was quality-assessed and quantified using a NanoDrop 8000 spectrophotometer ...
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bioRxiv - Immunology 2023Quote: ... and on-column DNA digestion with the RNase-free DNase Set (Qiagen, Cat. 79254). All further steps were performed by the Functional Genomics Center Zurich (FGCZ) ...
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bioRxiv - Developmental Biology 2024Quote: ... On-column DNase digestion was performed using RNase-Free DNase Set (QIAGEN, catalog #79254) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and included an on-column genomic DNA digestion step using RNase-free DNase (Qiagen). Reverse transcription and PCR were performed on 35ng of total RNA per well using AgPath-ID One-Step RT-PCR Reagents (4387424 ...
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bioRxiv - Microbiology 2024Quote: ... We performed on-column DNase digestion using the RNase-Free DNase Set (Qiagen 79254). We eluted samples in RNase-free water according to the manufacturer’s instructions and stored recovered RNA at −80°C until library preparation ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) and expression of the His-tagged Cas proteins was performed following the instruction of the protein purification kit (Qiagen, Valencia, CA, USA). Single colonies of transformed cells were cultivated overnight ...
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bioRxiv - Physiology 2021Quote: ... Total RNA was extracted from EVs (20 μg protein) using miRNeasy Mini Kit (Qiagen). RNA was then reverse-transcribed using TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: Total cellular RNA was extracted using AllPrep DNA/RNA/Protein mini kit (Qiagen, UK). Reverse transcription was carried out using kits from Invitrogen following the manufacturer’s instructions (SuperScript First-Strand Synthesis System for RT-PCR) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then isolated with Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen # 80004). Breast microstructures from 5 independent donors were exposed to vehicle (PBS and PBS plus DMSO) ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria isolates then underwent DNA isolation using AllPrep Bacterial DNA/RNA/Protein Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... in accordance with the manufacturer’s instructions that included the genomic DNA digestion with DNaseI (Qiagen). Isolated RNA from each sample (150 ng ...
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bioRxiv - Molecular Biology 2021Quote: ... On-column DNase digestion was performed by using an RNase-free DNase set (Qiagen #79254). The extracted RNA concentration was estimated using a NanoDrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: 72 µl Proteinase K digestion solution (63 uL Qiagen PKD buffer, 9 uL Proteinase K) was injected into the flow cell and incubated at 55°C for 30 min ...
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bioRxiv - Plant Biology 2019Quote: ... an on-column DNase digestion was incorporated according to the RNase-Free DNase Set (Qiagen) protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA extraction was performed according to the manufacturer’s protocol followed by DNase digestion (Qiagen #79254) and ethanol precipitation of RNA ...
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bioRxiv - Bioengineering 2021Quote: ... to extract RNA based on manufacturer instructions with an on-column DNase I digestion (Qiagen) for minimizing genomic DNA contamination ...
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bioRxiv - Biochemistry 2022Quote: ... On-column DNase digestion was performed by using an RNase-free DNase set (Qiagen #79254). 1 μg RNA was reverse transcribed by using the High-Capacity cDNA reverse transcription kit (Applied Biosystems #4368814) ...
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bioRxiv - Plant Biology 2021Quote: ... including on-column DNase digestion to remove any contaminating genomic DNA (#79254, Qiagen, Hilden, Germany). RNA was quantified using the Qubit RNA Assay Kit in a Qubit 2.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... with an additional on-column DNA digestion using the RNase-Free DNase Set (Qiagen; 79254). RNA was depleted of rRNA and RNA-seq libraries prepared as for the 4sU-seq.
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bioRxiv - Genomics 2019Quote: ... On-column DNase I digestions were performed with the Qiagen RNase-free DNase Set (Qiagen). RNA was eluted with 200 μL RNAse-free water (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... with an additional on-column DNA digestion using the RNase-Free DNase Set (Qiagen; 79254). cDNA was synthesised from 1 μg RNA using SuperScript II Reverse Transcriptase (ThermoFisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... An on-column DNase I digestion was carried out using RNase-free DNase set (QIAGEN). RNA was eluted in 30 μL of RNase-free water ...
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bioRxiv - Immunology 2020Quote: ... DNA was eliminated using DNase digestion/ treatment using RNase-Free DNase Set (Qiagen, Hilden, Germany) by adding it directly onto the columns ...