Labshake search
Citations for Qiagen :
201 - 250 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... After the SrfI digestion and gel purification of the large fragment of pcutFOG (Qiagen Gel purification kit; Qiagen, Hilden, Germany), T4 DNA ligase (NEB Lab ...
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bioRxiv - Genomics 2019Quote: ... RNA was collected using Qiagen RNeasy Mini Kits with Qiashredder and on-column DNase I digestions with Qiagen RNase-free DNase Set (Qiagen).
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bioRxiv - Developmental Biology 2020Quote: ... DNA was finally purified by reverse crosslinking at 65°C overnight followed by proteinase K (0.5 or 1 mg/ml, respectively) digestions and purifying with MinElute® PCR purification kit (Qiagen).
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bioRxiv - Genetics 2020Quote: The telomere-specific probe was obtained by digestion of pBL423 with EcoRI followed by gel extraction using the QIAquick Gel Extraction Kit (Qiagen). Plasmid pBL423 was a kind gift from M.P ...
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bioRxiv - Biochemistry 2020Quote: ... The restriction digestion efficiency was validated using 1% 1× TBE agarose gel electrophoresis and purified with a QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Genetics 2020Quote: ... was linearized by restriction enzyme digestion with AgeI and then purified using a MinElute PCR Purification Kit (Qiagen, Duesseldorf, Germany). Cas9 mRNA was produced by in vitro transcription using a mMESSAGE mMACHINE T7 Ultra Kit (Ambion ...
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bioRxiv - Genetics 2020Quote: Total RNA was extracted from T47D and RT4 cells using RNeasy Mini Kit with an on-column DNase digestion (Qiagen) and treated with Ribo-Zero (Illumina) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... We extracted total RNA in the brain of sticklebacks and performed a DNase digestion using the miRNeasy Mini Kit (Qiagen) combined with the RNase-Free DNase Set (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted using a QIAGEN RNeasy Mini Kit followed by two rounds of DNase digestion: one on-column using the QIAGEN RNase-Free DNase Set (79254, QIAGEN); and one off-column using Invitrogen’s TURBO DNA-free Kit (AM1907 ...
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bioRxiv - Microbiology 2021Quote: ... virion RNA was purified using the RNeasy Mini Kit with on-column DNase digestion with RNase-Free DNase I (Qiagen). The copies/μL in the virion standard were estimated by triplicate measurements using the Abbott RealTime SARS-CoV-2 assay (Abbott m2000 Molecular Platform) ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA was isolated from the lysate using on-column digestion of genomic DNA via RNeasy Micro Kit (CC# 74004; Qiagen). RNA quality was assessed using High sensitivity RNA TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick change PCR reactions were adapted by replacing the DpnI digestion step with a gel extraction protocol (QIAquick Gel Extraction Kit, Qiagen). Restriction digestion reactions for preparation of vector and insert DNA was processed using BamHI-HF ...
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bioRxiv - Microbiology 2023Quote: ... was used for total RNA extraction according to the manufacturer’s protocol which included a first DNase treatment using the On-column DNase I digestion kit (Qiagen, Switzerland). Extracted total RNA was subjected to a second DNase treatment using Promega RQ1 at 1 unit/μg of RNA ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from a 200 uL blood sample for each subject using QIAamp DNA Blood Mini kits with RNaseA digestion (Qiagen). SNV genotyping was performed using Illumina Infinium ImmunoArray-24 v2.0 BeadChips (InfiniumImmunoArray-24v2-0 ...
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bioRxiv - Genetics 2023Quote: ... which in summary used a freeze-thaw cycle and zymolyase cell wall digestion steps were coupled with Qiaprep spin miniprep kit (Qiagen) column purification ...
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bioRxiv - Immunology 2023Quote: ... 1hr after first Gal dose and AC1 were used for RNA extraction with the RNeasy kit with on column DNA digestion (Qiagen). Library preparation was performed using TruSeq Stranded Total RNA with Ribo-Zero Globin and sequencing was done with an Illumina HiSeq4000 with >20M reads/samples ...
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bioRxiv - Cancer Biology 2023Quote: ... The material stored in Qiazol was heated at 65°C for 5 min and RNA was extracted using the RNeasy Lipid Tissue Kit including the optional DNase digestion (Qiagen). RNA quality and concentration were assessed using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Systems Biology 2023Quote: ... Total RNA from both mycelial and conidial samples were further cleaned up using RNeasy Mini Kit with on-column DNase digestion (Qiagen) per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from mouse embryos were isolated using RNeasy plus micro kit with on-column DNase I digestion method (QIAGEN). cDNA libraries were produced by using an Ovation RNAseq amplification kit Ver ...
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bioRxiv - Pathology 2023Quote: ... After DNase I digestion and removing the ribosomal RNAs (rRNAs) from total RNA using the QIAseq FastSelect-rRNA Plant Kit probe (QIAGEN), several Adenine bases were tailed at the 3’ end of the remaining RNAs according to Liefting et al (47) ...
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bioRxiv - Developmental Biology 2021Quote: ... including on-column DNase I digestion (QIAGEN, cat. #79254). Complementary DNA was synthesized from 1 μg total RNA using the Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... including digestion with the RNase-Free DNase Set (Qiagen). cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Systems Biology 2019Quote: ... using on column DNA digestion (Qiagen, Cat No. 79254). RNAseq libraries were sequenced on an Illumina HiSeq 4000 system at the QB3 Vincent J ...
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bioRxiv - Genomics 2020Quote: ... RNAse A and proteinase K digestion followed by Qiagen Genomic-tips according to the manufacturer instructions ...
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bioRxiv - Bioengineering 2020Quote: ... along with an on-column DNase digestion step (Qiagen) to extract RNA with minimal genomic DNA contamination ...
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bioRxiv - Microbiology 2021Quote: ... and its associated RNase- Free DNase digestion set (QIAGEN), following the manufacturer’s protocol for mammalian cells ...
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bioRxiv - Systems Biology 2019Quote: ... DNA was removed by on-column DNase digestion (Qiagen). Purified RNA was quantified using a Nanodrop 2000 spectrophotometer.
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bioRxiv - Immunology 2019Quote: ... following the protocol with on column DNAse Digestion (Qiagen).
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bioRxiv - Immunology 2020Quote: ... with a 15-minute on-column DNase digestion (Qiagen) to remove genomic DNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by DNA digestion and column purification (Qiagen RNeasy). RNA quality was confirmed spectrophotometrically and by agarose gel electrophoresis.
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bioRxiv - Genetics 2021Quote: ... DNA was removed by on-column DNase digestion (Qiagen). Purified RNA was quantified using a Nanodrop 2000 spectrophotometer and RNA Fragment Analyzer (Agilent Technologies) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial digestion was performed using RNAprotect Bacteria Reagent (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... RNA was purified using on-column DNase digestion (Qiagen).
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bioRxiv - Physiology 2023Quote: ... following on-column DNase digestion (Qiagen, Cat. No 79254) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was column purified with DNase digestion (Qiagen; 79254) according to the RNeasy Mini Kit protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by overnight digestion using proteinase K (Qiagen #19133). Total RNA was then extracted using Tri Reagent protocol according to the manufacturer instructions (Sigma Aldrich # T9424) ...
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bioRxiv - Plant Biology 2024Quote: ... including an on- column DNase digestion (Qiagen, Hilden, Germany), from shaved hypocotyls from accession S ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was extracted 48 hours post transfection with All Prep RNA/Protein Kit (Qiagen, USA). Protein concentrations were determined by Lowry assay (Bio-Rad ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... protein extraction was performed using the Allprep RNA/Protein Kit (80404 Qiagen Inc., Hilden, Germany). Proteins (10–20 µg ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was isolated from powdered tissues using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Protein pellets were lysed in HES-SDS buffer (20 mM HEPES [pH 7.4] ...
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bioRxiv - Cell Biology 2023Quote: Cells were collected for total protein using Qproteome Mammalian Protein Prep Kit (Cat. 37901, Qiagen). Lysates were quantified for protein expression utilizing the WES system (ProteinSimple ...
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bioRxiv - Immunology 2021Quote: ... 5U uracil DNA glycosylase (UDG) was added for digestion at 37℃ for 40min and product was purified with MinElute PCR Purification Kit (Qiagen, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA isolation and on-column DNase digestion from cells were performed basing on the manufacturer’s protocol RNeasy Plus Mini Kit (Qiagen Cat #: 74134). 1 ug of RNA was used to synthesize the cDNA according to the one step cDNA synthesis kit from QuantaBio (Cat# ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was isolated according to the manufacturers protocol with an additional DNA-se digestion step using the Qiagen RNAse free DNAse kit (Qiagen, Germany). RNA quality and concentration of all samples were determined using the Agilent Bioanalyzer before and after the LCM procedure.
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bioRxiv - Microbiology 2022Quote: ... residual genomic DNA contamination was removed by an on-column DNAse digestion performed using the RNAse-free DNAse I kit (Qiagen #79254) following the manufacturer’s protocol at 37°C for 1 hr ...
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bioRxiv - Genomics 2023Quote: ... To then quality control the MNase digestions: 125 μL of each sample were cleaned-up using QIAquick PCR Purification Kit (QIAGEN, 28106) and assessed by agarose gel and Bioanalyzer ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was harvested in QIAZOL and prepared using the Qiagen miRNEASY Kit with DNase On-column digestion (Qiagen, Hilden, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: We used Allprep DNA/RNA/Protein mini kit (Qiagen) for DNA isolations from FNAs and QIAamp DNA Kit for blood and plasma ...
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bioRxiv - Biochemistry 2019Quote: ... RNA extraction with All Prep RNA/Protein Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... RNA and Allprep DNA RNA Protein Mini Kit (QIAGEN) extraction kit according to the manufacturer’s specifications.