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Citations for Qiagen :
601 - 650 of 5525 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... APC-negative cells were single-cell sorted by FACS into TCL buffer (QIAGEN) containing 1% 2-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNeasy columns (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from cells with RNeasy kit with Qiashredder cell disruption (Qiagen) and RNase-free DNase Set (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested and resuspended immediately in RNAprotect Cell Reagent (Qiagen, Cat# 76526) for RNA stabilization ...
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bioRxiv - Microbiology 2019Quote: ... Both uninfected cells and infected cells were harvested by incubating the cells on ice and quickly resuspending in RNAprotect Bacteria Reagent (Qiagen, catalog no. 76506), then storing at −80 °C until use ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions were incubated with red blood cell lysis (RBC lysis buffer, Qiagen) for three minutes before pelleted by centrifugation (400 x g ...
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bioRxiv - Immunology 2021Quote: Sorted cells (800 cells) were lysed in a guanidine thiocyanate buffer (TCL buffer, QIAGEN) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated by immunomagnetic selection and resuspended in RNAprotect Cell Reagent (Qiagen); RNA was extracted with the RNeasy mini kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: Long DNA was extracted from BmN4 cells using Blood & Cell Culture DNA Kit (QIAGEN), sequencing libraries were prepared using Rapid Sequencing Kit (Oxford Nanopore) ...
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bioRxiv - Genomics 2023Quote: ... genomic DNA was extracted from ∼106 cells using the Gentra Puregene Cell Kit (Qiagen) and sonicated at 4°C using a Bioruptor Pico sonication device (Diagenode ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Treated cells were processed using the FastLane Cell Multiplex Kit (Qiagen product code 216513). Threshold cycle numbers were determined using an AriaMx Real-time PCR System (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were removed from the plate with 250µL of RNAprotect Cell Reagent (Qiagen 76104) and stored at -20°C for at least 24 hours prior to extraction ...
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bioRxiv - Immunology 2024Quote: ... KPctrl and KP KO cell lines using DNA Blood and cell culture kit (Qiagen) following the manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells transfected with NCm (Qiagen) or NCi (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... and REPLIg Single Cell (Qiagen). The study was performed on five primary leukemia samples with known copy number variation based on bulk sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... H1299 cells (control siRNA (Qiagen), CD59- or flotillin-depleted ...
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bioRxiv - Cell Biology 2021Quote: ... Qproteome Cell Compartment Kit (Qiagen) was used to separate membrane ...
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bioRxiv - Cell Biology 2021Quote: ... coli BLR(pREP4) cells (Qiagen) cultured in DYT medium (1% tryptone ...
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bioRxiv - Immunology 2023Quote: ... resuspended in cell RNAProtect (Qiagen) and stored at -40°C until processing for TCR sequencing.
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bioRxiv - Cell Biology 2023Quote: ... Cell Lysis Solution (Qiagen #158906), RNase A Solution (Qiagen #158922) ...
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bioRxiv - Immunology 2021Quote: Total cell RNA was extracted using the miRNeasy Tissue/Cells Advanced Mini Kit (Qiagen, #217604). Purified RNA yields and quality were assessed via Agilent 2100 Bioanalyzer using the Agilent 6000 RNA Pico chips (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Total DNA was amplified from single cells with the REPLI-g Single Cell Kit (Qiagen) and genotyping was performed with NovaTaq Hot Start Master Mix (Millipore ...
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bioRxiv - Immunology 2019Quote: ... we analyzed cell clusters containing at least 25 cells using Ingenuity Pathway Analysis (QIAGEN Inc.). The top 5000 DEGs corresponding to the identified cell clusters were investigated with the gene enrichment analysis tool Enrichr167,168 ...
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bioRxiv - Immunology 2020Quote: Sorted cells (300-800 cells) were lysed in a guanidine thiocyanate buffer (TCL buffer, Qiagen) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: Control cells and COG5-silenced cells were lyzed and total RNA purified by QIAshredder (Qiagen) and extracted with RNeasy Protect Mini Kit (Qiagen ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... cells were washed with PBS and then cells were lysed in 350µl RLT buffer (Qiagen). Total RNA was extracted through columns using RNeasy kit (Qiagen ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... cells were washed with PBS and then cells were lysed in 350µl RLT buffer (Qiagen). Total RNA was extracted through columns using RNeasy kit (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: Cell lines were lysed directly in the cell culture plate by adding Qiazol reagent (Qiagen) and RNA isolation followed manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... cells were fixed for immunocytochemistry or cells were lysed in RNA lysis buffer (Qiagen, MD) and the lysate stored at −80°C until RNA purification ...
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bioRxiv - Immunology 2021Quote: ... up to 1,500 cells were collected from each cell subset directly into buffer TCL (Qiagen) with 1% β-mercaptoethanol (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: DNA was isolated from transduced K562 cells using Gentra Puregene Cell Kit (Qiagen, Hilden, Germany), according to the protocol ...
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bioRxiv - Bioengineering 2022Quote: ... was isolated from cells and cell-derived EVs using the miRNeasy kit (QIAGEN, Germantown MD). The RNA was eluted with 50 μl of nuclease-free H20 ...
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bioRxiv - Cancer Biology 2023Quote: The genomic DNA of harvested cells were isolated using Blood & Cell Culture Maxi kit (Qiagen). The PCR amplicon spanning the two sgRNAs were generated with PCR using Q5 High Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: RNA was harvested from cell pellets of 1 million cells using the RNeasy Mini kit (Qiagen). DNA was digested using the TURBO DNA-free Kit (Ambion ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were trypsinized and 0.5 × 106 cells were plated directly with Effectene transfection mixture (Qiagen, 301427) on 0.1% gelatin (in-house production ...
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bioRxiv - Neuroscience 2019Quote: Total RNA was isolated from NIH3T3 cells using the FastLane Cell One-Step Buffer Set (Qiagen). The mouse embryo brains at E10.5 were dissected in cold PBS ...
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bioRxiv - Cancer Biology 2020Quote: Whole bone marrow cells were subjected to red blood cell (RBC) lysis (RBC lysis solution, Qiagen) and resuspended in IMDM media containing 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... extracted cell clusters were incubated with a cell lysis mixture (20µL RLT Plus buffer (Qiagen 1053393), plus 1µL of spike-in RNA (1:250,000) ...
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bioRxiv - Microbiology 2020Quote: ... total RNA from infected cells and uninfected cells were extracted using Qiagen RNeasy mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells and TLR-MCV1 cells were transfected using PolyFect Transfection Reagent (Qiagen, Cat No. 301105) with 400 ng of plasmid in a 24-well plate according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... we isolated DNA from single cell suspensions or single cell pellets using a DNeasy Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... virus medium was aspirated and cells were harvested using 400 µL of RNAprotect Cell Reagent (Qiagen). RNA was extracted using the Qiagen RNAeasy Mini kit ...
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bioRxiv - Microbiology 2019Quote: ... cytoplasmic and host membrane-containing cell fractions were obtained using the Qproteome Cell Compartment Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... DNA from ~7×106 cells was extracted using a Blood & Cell Culture DNA Midi Kit (QIAGEN) and amplified to examine the coverage of mutant cell libraries ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from sorted cells using a Gentra Puregene Cell kit (Qiagen, catalog no. 158767). Immunoglobulin heavy-chain family–specific PCRs were performed on genomic DNA samples using primers in FR1 and JH as described previously (41 ...
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bioRxiv - Immunology 2021Quote: The sorted cells were washed thoroughly and the cell pellet was suspended in RLT Buffer (Qiagen) and stored at -800C until RNA extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and then cells were lysed in 350 μL RLT buffer (Qiagen). Total RNA was extracted through columns using RNeasy kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA from A549 cells (1 × 106 cells) was extracted using miRNeasy Mini kit (Qiagen, Denmark) according to the manufacturer’s instructions and eluted in 60 µl of nuclease free water ...
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bioRxiv - Genetics 2019Quote: ... RNA from collected cell types and (cancer) cell lines was isolated using the miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from FACS-sorted cells using Qiagen RNA Mini (>100,000 cells, Qiagen 74104), RNA Micro (<100,000 cells ...