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Citations for Qiagen :
101 - 150 of 10000+ citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... human sputum cells or mouse lung tissue using an RNeasy kit (Qiagen). 2μg was utilised for cDNA synthesis using the Omniscript RT kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from mouse cells using the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Neuroscience 2020Quote: ... Fold enrichment for each primer set was normalized to input and reported relative to a negative control gene desert region (mouse Igx1a (Qiagen). Primers ...
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bioRxiv - Cancer Biology 2019Quote: ... T cells were sorted directly into 250μl RNAprotect buffer (Qiagen), spun down for 1 minute at 2000 RPM ...
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bioRxiv - Cancer Biology 2021Quote: DNA was extracted from macro-dissected regions (Extended Data Fig.1E) using GeneRead DNA FFPE kit (Qiagen) and submitted to Adaptive Biotechnologies (Seattle ...
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bioRxiv - Zoology 2022Quote: The RAIS amplified BLV-host chimeric regions were then purified using the QIAquickR PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co. ...
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bioRxiv - Immunology 2021Quote: ... we extracted total RNA from CD4+/CD8+/CD3+ T cells (from spleen or bone marrow) of Foxp3-GFP or C57BL/6 mice using RNeasy Micro Kit (Qiagen) and cleaned from excess DNA with DNAse 1 enzyme (Promega) ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from circulating PD-1+ and PD-1− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing.
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bioRxiv - Immunology 2021Quote: ... DNA was isolated from sorted IFNγ+ and IFNγ− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from flow cytometry-sorted T cells using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... genomic DNA purified from CD8 T cells and bisulfite converted using the EpiTect Bisulfite Kit as per the manufacturer’s instructions (Qiagen, Inc.). Bisulfite-converted DNA was PCR amplified and cloned with the TOPO TA cloning kit (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from freshly isolated T-cells on day 7 of treatment from spleens using RNeasy Kit (Qiagen). For each group ...
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bioRxiv - Immunology 2020Quote: ... was isolated from previously sorted T cells and monocytes (CD19-CD3+/CD14+)30 using the QiaAmp DNA mini kit (Qiagen), and the concentration was measured on Nanodrop.
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Cancer Biology 2023Quote: ... deep sequencing genomic DNA was isolated from PBMC or CD4+ and CD8+ sorted T cell populations following the DNeasy Blood and Tissue Kit (Qiagen) protocol ...
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bioRxiv - Immunology 2024Quote: TET2 knockout efficiency was confirmed by isolating genomic DNA from CAR T-cells at Day 7 using the dNeasy Blood & Tissue Kit (Qiagen). PCR of genomic DNA was performed with TET2 Forward Primer 5’-TCCCTGAGTCCCAGTCCATC-3’ and Reverse Primer 5’-TCAGGAATGGCCAGGTTCTG-3’ using MyTaq Red 2X Mix (Meridian Bioscience) ...
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bioRxiv - Immunology 2024Quote: ... T-cell pellets were resuspended in 350 µL of RLT+ buffer from the Qiagen RNeasy plus mini kit (Qiagen, 74136) and RNA was extracted according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Immune cells were FACS-sorted for APC-enriched samples or T cell-enriched samples and RNA was subsequently extracted (RNeasy Mini Kit, Qiagen). RNA was analyzed on an nCounter MAX Analysis System (Nanostring ...
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bioRxiv - Cancer Biology 2019Quote: The cDNA of wild-type ecATCase and regulatory chain of ecATCase-holo were amplified by PCR (Qiagen Kit) using BL21(DE3 ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was isolated from mouse B cells using the DNeasy kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Immunology 2022Quote: ... and the cDNA was loaded onto the “Mouse Toll-Like Receptor Signaling Pathway” RT2 Profiler PCR Array according to the manufacturer instructions (Qiagen, Valencia, CA). Expression of the target genes were normalized to the geometric mean of Ct values of the two housekeeping genes Gusb and Hsp90a using the ddCt method (n = 3) ...
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bioRxiv - Microbiology 2021Quote: ... the synthetic T5 promoter region from pQE60 (Qiagen), the E ...
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bioRxiv - Immunology 2021Quote: Purified CD4+ T cells were homogenized using the Qiashredder (Qiagen, 79654) and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and the 200-400 bp region was excised and purified using a QIAquick Gel Extraction Kit (#28704, Qiagen). qPCR was performed using Power SYBR® Green PCR Master Mix (#4367659 ...
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bioRxiv - Neuroscience 2022Quote: We isolated RNA from the right hemisphere brain regions described above using the RNeasy 96 kit (Qiagen, 74181) and a TissueLyser II (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μL of bisulfite treated DNA was amplified for the regions of interest using PyroMark PCR kit (Qiagen) with final primer concentration of 0.4 μM ...
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bioRxiv - Neuroscience 2023Quote: ... The CA1 region was microdissected and RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) with additional on-column DNase I digestion ...
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bioRxiv - Immunology 2023Quote: ... and the region of interest (around 480 bp) was excised and purified using QIAquick Gel Extraction Kit (Qiagen), then eluted in 20 µl EB buffer.
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bioRxiv - Microbiology 2024Quote: ... the 300-500 bp region was excised and DNA was extracted using the MinElute Gel Extraction Kit (Qiagen).
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bioRxiv - Immunology 2020Quote: ... Up to 8 colonies were selected for each TCRα and TCRβ sequence for each of the KRASG12V/D-specific T cell clones and DNA was isolated using a QIAprep Spin Miniprep Kit (QIAGEN, 27104) and sequenced using the M13F and M13R primers ...
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bioRxiv - Immunology 2020Quote: ... and HLA-C sequencing for each T cell clone and the control sample and DNA was isolated using a QIAprep Spin Miniprep Kit (QIAGEN, 27104). Sanger sequencing was performed using the M13F and M13R primers (Genewiz) ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from a tumor-reactive T cell culture using a Qiagen RNeasy Micro kit (Qiagen GmbH, Hilden, Germany). 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: Total genomic DNA from the sorted CD8+ T cells in the indicated groups were extracted using QIAamp DNA Micro Kit (Qiagen, 56304), eluted in a final volume of 50µL buffer AE and submitted for amplification and sequencing of the TCRβ region ...
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bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Insertion of the dcas9/lacI cassette at the desired chromosomal location in strain CJW_Bb362 was confirmed by polymerase-chain reaction (PCR) analysis of isolated total genomic DNA (DNeasy Blood & Tissue Kit, Qiagen), using the following primer pairs ...
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bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments that were generated by the polymerase chain reaction (PCR) were purified using the PCR Purification Kit (Qiagen), Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Pathology 2022Quote: Total RNAs were obtained from cells or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from mouse MLL-AF9 cells by RNeasy Micro Kit (Qiagen), 72 hours after transduction of the cells ...
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bioRxiv - Molecular Biology 2022Quote: ... for mouse samples and cell lines or with miRNeasy Mini Kit (Qiagen, Hilden, Germany) for human tissue samples ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cells and mouse tumors using the miRNeasy kit (Qiagen, #217004) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested by centrifugation at 4°C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
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bioRxiv - Immunology 2020Quote: ... Sorted T cells were lysed in Buffer RLT Plus (Qiagen, Hilden, Germany) with 1% β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Microbiology 2023Quote: ... selected genomic regions were PCR amplified and cleaned (Qiagen) and sent for sequencing to the Dartmouth College Genomics and Molecular Biology Shared Resources Core ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using a QuantiFast SYBR Green PCR kit (QIAGEN, 204056) on a StepOnePlus real-time PCR system (Applied Biosystems) ...