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Citations for Qiagen :
1 - 50 of 10000+ citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: A region-specific polymerase chain reaction (PCR) was performed for the S-COMT promoter region using the PyroMark PCR Kit (Qiagen, Hilden, GER) according to manufacturer’s instructions with previously published primers (F ...
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bioRxiv - Immunology 2024Quote: ... total mRNA was isolated from mouse T cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2020Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cβ primer ...
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bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
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bioRxiv - Genetics 2022Quote: ... mouse brain and brain region tissues using the miRNeasy kit (QIAGEN, # 217004) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the variable region genes from each single cell were amplified in a reverse transcriptase polymerase chain reaction (RT-PCR: QIAGEN, Germany) using a cocktail of sense primers specific for the leader region and antisense primers to the Cγ constant region for heavy chain and Cκ and Cγ for light chain ...
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bioRxiv - Neuroscience 2019Quote: Total RNA was extracted from distinct mouse brain regions using the RNeasy Mini Kit (QIAGEN). The QuantiTect Reverse Transcription Kit (QIAGEN ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from cultured in vitro or in vivo primed T cells or ex vivo sorted T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... cDNA products were then used in nested PCR for heavy/light chain variable region amplification with HotStarTaq Plus DNA Polymerase (QIAGEN # 203643) and specific primer sets described previously 34,35 ...
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bioRxiv - Immunology 2020Quote: A RT2 profiler array for mouse inflammatory cytokines and receptors (Qiagen, USA) was used according to manufacturer recommendations to measure the expression of 84 inflammatory genes (Table S1) ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted from regions containing mainly malignant cells using Allprep RNA/DNA kit from Qiagen.
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bioRxiv - Immunology 2021Quote: ... We then amplified heavy and light chain sequences from the WTA products utilizing pools of partially degenerate pools of V region specific primers (Qiagen HotStar Taq Plus). Heavy and light chain amplifications were carried out separately ...
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bioRxiv - Immunology 2020Quote: ... or CD4+ T cells was extracted using the RNeasy micro kit (Qiagen) followed by cDNA synthesis using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from isolated CD8+ T cells (RNeasy kit, Qiagen) and 300 ng of RNA were used for cDNA synthesis (First-Strand Synthesis kit ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from murine T cells via RNeasy Kit (Qiagen) with DNase digestion (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... and CD95+ CD4 T cells using a RNeasy plus mini kit (QIAgen). Isolated RNA sample quality was assessed using a BioAnalyzer RNA pico assay (Agilent Technologies Inc. ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was extracted from the same tumor region as the single-cell analyses using the Qiagen AllPrep kit and matched normal blood using DNeasy kit (Qiagen). Briefly ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... we prepared total RNA from dissected wildtype mouse brain regions using Qiazol (Qiagen) and prepared cDNA using the SuperScript III first-strand synthesis kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from neuropathologist annotated regions containing >60% cancer cells using Qiagen kits (Qiagen, Sussex, UK). Paired end ...
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bioRxiv - Immunology 2024Quote: ... total RNA was extracted from T cells by using RNeasy mini kit (QIAGEN) and reverse-transcribed into cDNA with Superiorscript III reverse transcriptase (Enzynomics ...
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bioRxiv - Immunology 2024Quote: T cells were subjected to RNA extraction utilizing the RNeasy Micro Kit (QIAGEN). RNA from the renal cortex was isolated with the NucleoSpin Kit (Macharey-Nagel ...
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bioRxiv - Microbiology 2019Quote: ... flanking region PCR products were gel purified (QIAEX II Kit; Qiagen), digested with XbaI (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and DNA was extracted from the same cells from neuropathologist annotated tumour regions (>60% cancer cells) using appropriate Qiagen kits (Qiagen, Sussex, UK). Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Immunology 2021Quote: ... total RNA of CD8+ human T cells was extracted by miRNeasy Mini Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from sorted CD4+ T cells using the RNeasy Mini kit (Qiagen). A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... for mouse cells or the RNeasy kit (Qiagen) for human cells ...
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bioRxiv - Genetics 2021Quote: Relative telomere length of MDS patients was measured by quantitative polymerase chain reaction (qPCR) as previously reported.8 K562 cell DNA was extracted using the QIAamp Blood Mini kit (Qiagen). Telomere length was measured by two orthogonal methods ...
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bioRxiv - Clinical Trials 2019Quote: Genomic DNA from 1-10 Million CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen). In some experiments ...
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bioRxiv - Evolutionary Biology 2022Quote: ... QuantiTect Probe RT-PCR (reverse transcription polymerase chain reaction) Kit (QIAGEN, Hilden, Germany) was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: Polymerase Chain Reactions (PCRs) were performed using Type-it Microsatellite PCR Kit (Qiagen) in 10 µl reactions ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from CD4+ T cells using the DNeasy blood and tissue kit (Qiagen), according to manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... The total RNA of sorted T cells was extracted with an RNeasy Micro kit (Qiagen) and reverse transcribed using reverse transcribed using SuperScript III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... primary T cells and BJ-5at fibroblasts using the RNeasy Plus Mini Kit (Qiagen. #74136), reverse transcribed to first-stand cDNAs using iScript™ cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was extracted from sorted T cell populations with mini RNA-easy Kit (Qiagen). Equal amounts of total RNA from different groups were used for the assay ...
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bioRxiv - Immunology 2022Quote: ... DNA for library preparation was extracted from CD8+ T-cells using FlexiGene DNA Kit (Qiagen). 150 ng aliquots of obtained DNA were used as input to prepare each out of four TRA libraries ...
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bioRxiv - Immunology 2022Quote: ... CD4+/CD8+ T cells and Tfh from indicated organs using RNeasy Mini Kit (Qiagen Inc.). cDNA synthesis was performed using Superscript III First Strand Synthesis System (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... isolate DNA of CAR-T cell pellets with DNEasy Blood & Tissue Kit (Qiagen catalogue # 69506), perform PCR amplification with appropriate forward and reverse primers to TSC2 S1364A Δ insertion and then submit PRC products for sequencing (Genewiz ...
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bioRxiv - Immunology 2023Quote: T-cells or homogenized colon were suspended in RLT buffer (RNAeasy kit, Qiagen, Valencia, CA), RNA was extracted (RNAeasy ...
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bioRxiv - Immunology 2023Quote: ... RNA was extracted from CAR T cells using a Qiagen RNeasy Mini Kit (Qiagen; #74104) and RNA was quantified by NanoDrop Spectrophotometer (ThermoFisher) ...
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bioRxiv - Genomics 2022Quote: ... Age-associated regions were amplified using the PyroMark PCR Kit (Qiagen, Hilden, Germany) with manufactures instructions ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
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bioRxiv - Molecular Biology 2019Quote: ... The cloned plasmid was amplified in DH5-Alpha cells and purified with QIAfilter™ Plasmid Maxi Kit (Qiagen, catalogue number 12243). Plasmid concentration was measured using Qubit (Thermofisher ...
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bioRxiv - Immunology 2020Quote: RNA from CD27/CD45RA defined CD4+ T cell subsets was isolated using the RNeasy kit (Qiagen) according the manufactures instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA extraction from murine CD4+ T cells was performed using the RNeasy Plus Mini Kit (Qiagen) and cDNA was generated using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted from the T cells using the DNeasy Blood and Tissue kit (Qiagen, 69504) following the manufacturer’s protocol ...