Labshake search
Citations for Qiagen :
201 - 250 of 301 citations for Lifeink 200 Collagen Bioink since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions contained 4 μl of cDNA (1:50 dilution) and 200 nM of each primer (supplementary table 1) and QuantiTect SYBR Green PCR master mix (Qiagen) or SensiFAST SYBR Hi-ROX kit (Bioline) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from approximately 200-300 μl of fecal sample using the MoBio PowerSoil HTP 96 DNA isolation kit (formerly MoBio, now Qiagen) on the Eppendorf EpMotion 5075 automated pipetting system according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Size selection was performed by extracting 200–400 bp DNA fragments before PCR amplification and DNA was extracted using MinElute cleanup kits (Qiagen). After PCR amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral RNA was extracted from 200 μL of supernatant using QIAamp Cador Pathogen on a QIAcube HT (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... Total DNA was extracted from 200 µl of the homogenate using the QIAamp DNA stool Mini kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions and stored at −20°C.
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bioRxiv - Evolutionary Biology 2021Quote: ... and 440 µL of the mixture (220 µL of phosphate-buffered saline, 200 µL of Buffer AL, and 20 µL of proteinase K [Qiagen]) was added to the Sterivex filter ...
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bioRxiv - Microbiology 2021Quote: ... 200 mg of thawed and mixed faecal samples were added to 1 ml of InhibitEX Buffer (QIAGEN, GmbH, Hilden, Germany) followed by the beat-beating step in TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 45 min after which 25 µl of proteinase K (20 mg/mL) and 200 µl Buffer AL (Qiagen) was added ...
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bioRxiv - Genetics 2020Quote: HBV DNA was extracted from 200 μL plasma using a QIA amp ® DNA Blood Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions and 50 μL of eluted DNA was stored at −70 °C until use ...
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bioRxiv - Genetics 2019Quote: ... Lysates were clarified by centrifugation at 12,000xg at 4°C and mixed with 200 µL of a slurry of Ni2+-NTA agarose (Qiagen #30210) that had been equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Microbial DNA was isolated from 200 μL of homogenized fecal material using the DNeasy PowerSoil Pro extraction kit (Qiagen, Germany) with bead beating in Qiagen Powerbead Pro plates (Cat No ...
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A Non-invasive, Biomarker Assay for Detecting Chronic Wasting Disease Pathology in White-tailed DeerbioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from 200 uL of serum using the miRNeasy Serum/Plasma Kit (Qiagen Inc., Valencia, California, USA) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... including either whole fecal pellets or 200-300 uL of contents diluted 1:10 (w/v) in sterile PBS using the MagAttract PowerMicrobiome Kit (Qiagen), and prepped DNA libraries as previously described (36) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from 200 μl of isolated EVs using the miRNeasy Serum/Plasma Advanced Kit (Qiagen, Crawley, UK) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Three rounds of agarose gel purification were performed to purify the fragments of 200~650 bp using QIAquick Gel Extraction Kit (Qiagen). The libraries were sequenced with Illumina HiSeq 4000 platform by BGI Genomics (Hongkong).
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bioRxiv - Plant Biology 2023Quote: ... RNA was eluted by incubation of the beads with 200 μl of RLT buffer from the RNeasy Mini Kit (Qiagen) for 2 min ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from a 200 uL blood sample for each subject using QIAamp DNA Blood Mini kits with RNaseA digestion (Qiagen). SNV genotyping was performed using Illumina Infinium ImmunoArray-24 v2.0 BeadChips (InfiniumImmunoArray-24v2-0 ...
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bioRxiv - Neuroscience 2023Quote: ... DNA isolation was performed on the cell pellets and 200 µl of the corresponding supernatants using the QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Bacterial strains were grown overnight in broth cultures (37° C, shaking at 200 RPM) and had genomic DNA extracted using a DNeasy Blood and Tissue kit (Qiagen). Genomic DNA was sequenced using the Illumina MiSeq at the Heflin Genomics Core at UAB ...
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bioRxiv - Systems Biology 2023Quote: ... 200 mg was extracted from each feces sample for DNA extraction by the QIAamp DNA stool Mini kit (Qiagen, Germany). 515F (5’-GTGCCAGCMGCCGCGGTAA -3’ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HBV-DNA was extracted from 200 μL of serum/CSF samples using a QIAamp Blood Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Short-fragment size-selection was performed by cutting out gel bands between 100-200 bp and subsequent purification using the QiaQuick gel extraction kit (Qiagen) according to the manufactureŕs instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Prior to RNA extraction individuals were homogenized in 200 μl homogenization buffer including 4 μl 1-Thioglycerol using a TissueLyser II (Qiagen) with a mixture of five 1 mm zirconia (BioSpec Products ...
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bioRxiv - Cancer Biology 2023Quote: ... by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2023Quote: ... The BmAgo3 immunoprecipitated beads were divided into two and incubated in buffer D with/without 200 μg/ml RNase A (Qiagen) at 30°C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: RNA from 100 µL of serum (35 µL for samples from pregnant dams and 15 µL for samples from P14-16 pups) or 200 µl conditioned media was isolated using the miRNeasy Serum/Plasma Advanced Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... equal amounts (200 µl) of FruK and Cra cell lysates were mixed with 100 µl of nickel-nitrilotriacetic acid beads (Qiagen). After overnight incubation at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2024Quote: ... 200 μL of the resuspension buffer as added.The resulting samples were purified using a QIAquick PCR purification kit (Qiagen, Germany).The sequence mixtures were prepared using a USB® Thermo Sequenase Cycle Sequencing Kit (Affymetrix ...
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bioRxiv - Molecular Biology 2023Quote: ... Each 1 mL washed schizont preparation was then distributed into five 200 μL aliquots and genomic DNA was extracted immediately using a QIAmp DNA Blood Mini Kit (Qiagen) following the manufacturer’s instructions with one exception ...
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bioRxiv - Microbiology 2024Quote: ... and pellet resuspended in 200 μL PBS before DNA was extracted using the QIAmp DNA Mini Blood Kit (Qiagen #163043063) as per the manufacturer’s protocol and DNA was eluted in 100 μL ...
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bioRxiv - Synthetic Biology 2023Quote: The pARP3 plasmid for methylation analysis was extracted from 200 mL of an overnight culture of Tol 5 transformant cells using the HiSpeed Plasmid Midi kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 100-200 μm thick sections were cut and used to extract total RNA using the RNeasy Mini Kit (Qiagen, USA). The RNA integrity number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA isolation from 200 mg of grounded tissue was performed with the RNeasy plant mini kit (Qiagen, Hilden, Germany) and the cDNA synthesis with the First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: DNA was extracted from 200 µL of the resuspended extracellular vesicles using the DNeasy Blood & Tissue Kit (Qiagen, cat# 69504) according to the instructions in the Purification of Total DNA from Animal Blood or Cells (Spin-Column Protocol) ...
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bioRxiv - Microbiology 2024Quote: ... with ReadyMade PrimeTime primers for SPI1 (Integrated DNA Technologies Inc, USA) and RT2 qPCR Primer Assay for Human GAPDH (cat# PPH00150F-200, Qiagen). Expression was quantified using ABI Sequence Detection software compared to serial dilutions of an SPI1 or GAPDH synthetic sequence gBlock (Integrated DNA Technologies Inc ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA yield was measured by spectrometry (NanoDrop ND-1000; peQLab) and 200 ng/μl were used for reverse transcription with QuantiTect®Reverse-Transcription Kit (Qiagen). We used primer pairs for immune system-related and metabolism-related genes (16 ...
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bioRxiv - Genomics 2021Quote: ... DNA corresponding to the size of mononucleosomes (100-200 bp) was re-isolated from a 3% agarose gel using MinElute Gel Extraction Kit (Qiagen, # 28604). As input ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately half the volume of each feces sample (100-200 mg) was immersed in 3 ml of Inhibit EX Buffer (QIAGEN, Hilden) and stored at room temperature ...
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bioRxiv - Immunology 2021Quote: ... a new empty 200 µL filtered tip was inserted to top channel outlet and 100 ul RNase easy lysis buffer (Qiagen, #74034) was used to lyse epithelial cells by quickly pressing and releasing the micropipette plunger 3 times ...
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bioRxiv - Microbiology 2020Quote: A 200 μL aliquot of Copan UTM® was processed through the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen, Cat# 937036) following manufacturer’s instructions on the QIAsymphony SP instrument ...
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bioRxiv - Microbiology 2020Quote: ... and the pellet containing virions was resuspended in 200 μl UltraPure water and added to the QIAGEN DNeasy PowerSoil Kit bead tubes (QIAGEN, Germany) for DNA extraction according to the manufacturer’s instructions with one exception ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected with 200 ng total endotoxin-free plasmid DNA using the Effectene transfection protocol (Qiagen Inc., Valencia, CA, USA). Plasmid DNA encoded for C-terminally EGFP- or DsRed-monomer tagged Panx1b fusion proteins ...
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bioRxiv - Systems Biology 2022Quote: RNA was extracted from the whole blood of PLHIV of the 200 HIV pilot study using the QIAGEN PAXgene Blood RNA extraction kit (QIAGEN, Netherlands) according to the instructions of the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... The band between 200-300bp was cut out and DNA was isolated from the gel using the QIAquick Gel Extraction kit (Qiagen, 28704), and 10-20nM of the eluents was submitted for paired-end Hi-seq 4000 for sequencing.
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bioRxiv - Genomics 2019Quote: Genomic DNA was extracted from 200 μL of whole blood using QIAamp DNA Mini kit (Qiagen, GmbH, D-40724 Hilden, Germany). The polymerase chain reaction (PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... A magnetic rack was used to remove the beads and 15 µl of each pre-cleared lysate was collected and added to 200 µl RLT buffer per sample (Qiagen, 74034) to serve as the input samples ...
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bioRxiv - Developmental Biology 2019Quote: ... size selected on a 1.3% agarose gel by cutting out fragments between 200-650 bp of size and extracted from the gel using QIAquick gel extraction kit (Qiagen, 28704). Samples were sequenced at Genomics Core Facility (GeneCore ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of each of the PCR products were pooled together to create the BarSeq sequencing library and 200 μL of the pooled library were purified using the MinElute purification kit (Qiagen, Germany). The final elution of the BarSeq library was performed in 30 μL in DNase and RNase free water ...
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bioRxiv - Molecular Biology 2020Quote: ... Each sample was tested by adding 12.5 μl QuantiTect® Multiplex PCR NoROX Kit (200 × 50 μl reactions, QIAGEN, Hilden, Germany), 1 µl IC-DNA ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of supernatant were used to extract DNA using the EZ1 apparatus according to the manufacturer’s recommendations (Qiagen, GmbH, Germany). Extracted DNA was stored at 4°C ...