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Citations for Qiagen :
151 - 200 of 301 citations for Lifeink 200 Collagen Bioink since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Small RNAs shorter than 200 nt were then removed from the poly(A) RNA using the RNeasy kit (Qiagen). This size selection was performed to prevent detection of extended cap structures that are known to be present in certain small RNAs ...
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bioRxiv - Cell Biology 2021Quote: ... 200 to 500 ng of RNA were used to prepare cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, 205311) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Serum viral RNA was extracted from 200 μl using the QiaAmp Min Elute Virus Spin kit (Qiagen, Cat# 57704) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from ~200 mg stool using the QIAGEN DNeasy PowerSoil HTP 96 Kit (Qiagen, Cat #12955-4) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from approximately 200-300μl of fecal sample using the MoBio PowerSoil HTP 96 DNA isolation kit (formerly MoBio, now Qiagen) on the Eppendorf EpMotion 5075 automated pipetting system according to manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... Feces samples were then homogenized in 200 μL 80% (v/v) methanol using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the 200 μl of bacterial suspension by using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... viral RNA was extracted from the suspension (200 μl) of concentrated particles using RNeasy Plant Mini Kit (Qiagen, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... was added to 200 mg of mycelium before grinding for 1.5 min at 30 Hz using a TissueLyzer (Qiagen). Total RNA was then extracted using a previously published protocol (Hallen et al. ...
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bioRxiv - Plant Biology 2022Quote: ... The m6A-modified RNAs were eluted using 200 μL of RLT buffer supplied in RNeasy Mini Kit (QIAGEN, 74106) for 2 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from 200 μl of all the clinical specimens with commercially available DNA extraction kit (Qiagen, USA). The DNA concentration was measured by Qubit 2.0 fluorimeter using Qubit dsDNA kit (Themofisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in tubes containing glass beads (200 mg, 0.4 mm) and were lysed using a TissueLyser LT (Qiagen). To minimize protein degradation ...
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bioRxiv - Immunology 2023Quote: ... and plasmid DNA from 200-500 µl of the cell suspension was purified using QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).
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bioRxiv - Systems Biology 2023Quote: ... Large fragments (200-400 bp) were extracted from a 1% agarose gel with QIAquick Gel Extraction Kit (Qiagen, Netherlands). The final library was sequenced as 85 bp long single-end reads on a NextSeq™ 550 (Illumina ...
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bioRxiv - Microbiology 2023Quote: We extracted nucleic acids from each 200 μL aliquot using the AllPrep PowerViral DNA/RNA kit (Qiagen, Hilden, Germany) using Glass PowerBead tubes included with the kit ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was diluted 3 times with buffer A (20 mM HEPES, pH 7.4, 200 mM NaCl, 1 mM Asp) and incubated with Ni-NTA resin (Qiagen) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The OsHV-1 µVar DNA was extracted from 200 µL of water using the QIAmp DNA mini Kit (QIAGEN). Quantitative PCR was performed with 5 µL of DNA as described 46.
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with 200 ng of extracted total RNA using a Qiagen OneStep RT-PCR Kit (Qiagen). The primers used were a forward primer (Hokkaido System Science ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 200 μl 100% ethanol was added dropwise while being gently vortexed and placed into a RNeasy Mini column (Qiagen), The remainder of the purification followed the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Oral fluid samples were prepared by homogenization of 200 μl sample (30 sec at 15 Hz) in a Tissuelyser II (Qiagen) followed by centrifugation (2 min at 10.000 rpm) ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries were size-selected (200-350 bp) with an agarose gel extraction using the MinElute Gel Extraction Kit (Qiagen, Germany). Sequencing was performed on Illumina HiSeq2500 by the Max Planck-Genome-centre Cologne ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA (200 ng) was used for quantitative PCR with the Human Cellular Senescence RT2 Profiler™ PCR Array (Qiagen) containing 84 key genes involved in the initiation and progression of the biological process causing cells to lose the ability to divide ...
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bioRxiv - Evolutionary Biology 2021Quote: ... fragment sizes of 200-400 bp and 400-600 bp were isolated for library construction using a MinElute Gel Extraction kit (Qiagen). We quantified DNA concentration in a Qubit2.0 ...
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bioRxiv - Genomics 2020Quote: ... and a library representation of >200 colonies per guide was determined prior to CRISPR library pool plasmid purification by Endofree Maxi (Qiagen). Coverage of the 818 guide library for Perturb-CITE-Seq as well as the extended control library was confirmed by PCR amplification of the guide sequence from the final library pool and deep sequencing on an Illumina MiSeq.
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bioRxiv - Evolutionary Biology 2019Quote: Total RNA was extracted from ground Arabidopsis leaf tissue and from 200 mg ground maize material using the RNeasy plant mini kit with on-column DNase digestion (Qiagen). The RNA-seq data of the A ...
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bioRxiv - Cancer Biology 2019Quote: ... previously exposed to normoxia or hypoxia for 16h in the presence or absence of BCP at either 20 μM or 200 μM (RNase easy plus mini kit from Qiagen). Libraries were prepared at the Genomics Core at the University of Louisville and sequencing was performed on triplicate biological replicates (Illunima NestSeq 500) ...
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bioRxiv - Genomics 2019Quote: ... or PUF-fusion (200 ng) in the presence of Attractene or Lipofectamine 3000 transfection reagents according to manufacturers’ instructions (Qiagen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Cecal contents (200 µl) were also used to extract DNA using QIAamp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany). E ...
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bioRxiv - Cancer Biology 2021Quote: Sufficient amount of DNA (50-200 ng) was extracted from fresh-frozen tumor tissue and blood samples following standard protocols from Qiagen. DNA was fragmented by sonication for NGS Truseq library preparation and sequenced paired-end reads of 2x150 bases with the Illumina HiSeqX platform ...
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bioRxiv - Neuroscience 2020Quote: ... DNase I pre-treated samples were all resuspended in 200 μl of PBS and total DNA was finally extracted with DNeasy Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded at 50% confluency in a 10 cm dish and transfected with 200 pmol siRNA (Qiagen, Germantown, MD) using RNAiMax Lipofectamine (Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Leaf samples of about 200 plants were pooled and nuclear DNA was extracted with a DNeasy Plant Mini Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: A total of 600 μL (three × 200 μL) of day 4 SARS-CoV-2 culture supernatant was used as input into the RNeasy Mini Kit (Qiagen) for RNA extraction with minor modifications ...
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bioRxiv - Genomics 2020Quote: ... Cells were seeded in 6-well plates at a density of 1×106 cells/mL and transfected with 200 ng of plasmid using the Effectene transfection kit (Qiagen). Cells were harvested following 3 days of incubation.
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bioRxiv - Genomics 2020Quote: ... The cells were harvested 48 hours post-transfection by adding 200 μl RLT buffer from the RNeasy 96 Kit (Qiagen) after PBS washing ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 1×108 promastigotes from logarithmic growth phase were re-suspended in 200 µl PBS and genomic DNA was purified using DNeasy Blood and Tissue kit from Qiagen and RNase A according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR reactions were prepared with RT2 SYBR master mixes and RT2lncRNA qPCR assays for DR5-AS (Qiagen Cat., LPH15855A-200), CAMTA-AS (Qiagen Cat. ...
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bioRxiv - Microbiology 2022Quote: ... were harvested into 200 μl PBS using a cotton swab and the DNA was extracted using a DNeasy Blood and Tissue Kit (QIAGEN) as per the manufacturers protocol ...
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bioRxiv - Biophysics 2022Quote: ... Both unselected and selected cells were harvested by centrifugation and resuspended into 200 μL of PBS and processed with a DNeasy Blood and Tissue kit (Qiagen) per the manufacturer’s instructions to obtain genomic DNA ...
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bioRxiv - Biochemistry 2022Quote: ... subsequently run on a 2% agarose gel and bands of the correct size (ca. 200 bp) were purified with the QIAquick Gel extraction Kit (Qiagen). From the columns ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we extracted high-molecular weight (HMW) DNA from 200 uL fresh blood using the MagAttract HMW DNA mini kit (Qiagen), following the Whole Blood protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were resolved on a 2% agarose gel and the fragments migrating within the range of 200 to 330 nt were excised and the DNA was extracted using the Gel Extraction Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... groups of five 15-day-old female flies of each genotype and culture condition were homogenized in 200 µl of PBS with a steel bead using a TyssueLyser LT (Qiagen) for 2 min at 50 Hz ...
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bioRxiv - Molecular Biology 2020Quote: ... run on a 2% agarose gel and a smear of 200-500bp was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... run on a 2% agarose gel and a smear of 200-500bp was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... eluates were supplemented with imidazole at a final concentration of 10mM and incubated for 2 hours with 200 µL of Ni-NTA agarose beads (Qiagen) that were prewashed in buffer C (20mM HEPES [pH 7.6] ...
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bioRxiv - Neuroscience 2021Quote: ... we collected 200 embryos at different development stages and extracted total RNA using the Micro RNeasy Kit (Qiagen, Germantown, MD). For NeuroD1 MASO ...