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Citations for Qiagen :
151 - 200 of 227 citations for Ion Exchange Chromatography since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Phosphopeptides were enriched using Immobilized Metal Affinity Chromatography (IMAC) with NTA Magnetic Agarose beads (Qiagen, Cat # 36113) stripped of Ni2+ and reloaded with Fe(III) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The RBD was purified from the expression media by metal-affinity chromatography using Ni-NTA beads (Qiagen). The Fabs were purified from the expression media using rProtein A Sepharose Fast Flow beads (GE healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... for 45 min and the supernatant was purified using affinity chromatography with a Ni-NTA column (Qiagen). Ni-NTA column bound with MBP-GLIC fusion protein was washed with 5 column volumes of Buffer B1 with 50 mM imidazole and 1% DDM ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was purified by Ni2+ immobilized metal affinity chromatography (IMAC) with Ni-NTA Superflow resins (Qiagen). Resins with bound cell lysate were washed with 10 mL (bed volume 1 mL ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was carried out at 4°C with a Ni-NTA sepharose column (Qiagen, Hilden, Germany). The column was pre-equilibrated with 50 mM NaH2PO4-buffer pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... proteins were expressed in Escherichia coli strain BL21 (DE3) and purified via nickel-nitrilotriacetic acid affinity chromatography (Qiagen) followed by ion exchange chromatography on an Äkta system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... coli cells grown at 37°C by addition of 0.2 mM IPTG and native protein purified using Nickel affinity chromatography according to the manufacturer’s protocol (Qiagen). For N-terminal His-tag removal ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were purified from the cell lysate by affinity chromatography using a Nickel-NTA column (Qiagen, Valencia, CA). A solution containing 20mM HEPES pH7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... and loaded onto an equilibrated (lysis buffer) immobilised metal affinity chromatography column (IMAC) (Ni-NTA Superflow Cartridge, Qiagen). The immobilised protein was washed with lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Cancer Biology 2019Quote: ... The lysates were clarified by centrifugation and proteins in the supernatant were purified using Ni-NTA chromatography (Qiagen) for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... and purified in buffer C (20 mM Tris, 100 mM NaCl, pH 7.5) by nickel-affinity chromatography (Qiagen) followed by size-exclusion chromatography on a Superdex 200 column in buffer D (20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... FasRΔ33 or FasR point mutants were separated from whole-cell lysates by Ni-NTA agarose chromatography (Qiagen, Inc). After three washing steps with lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... and the protein of interest was purified by affinity chromatography on a Ni-NTA column (Qiagen, Hilden, Germany), using a TRIS-HCl 50 mM ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was purified by Ni2+ immobilized metal affinity chromatography (IMAC) with Ni-NTA charged magnetic beads (Qiagen). Magnetic beads with bound cell lysate were washed with 200 μL of washing buffer for three times (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: Immobilized metal affinity chromatography (IMAC) beads for phosphopeptide enrichment were prepared by stripping Ni-NTA Superflow beads (Qiagen) with 100 mM EDTA (pH 7.5) ...
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bioRxiv - Microbiology 2022Quote: ... filtered and the secreted recombinant proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen; Cat.# 30230). Specifically ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged talin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged vinculin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression and affinity purification using immobilized metal affinity chromatography (IMAC) were carried out according to manufacture’s instruction (Ni-NTA agarose, Qiagen). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... The released nanobody was purified via standard immobilized metal affinity chromatography (IMAC) with Ni-NTA agarose (Qiagen, MD, USA). The product was confirmed via SDS-PAGE analysis.
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bioRxiv - Biochemistry 2020Quote: ... and the recombinant protein was purified by gravity-flow chromatography using a nickel-charged resin Ni-NTA Agarose (QIAgen) equilibrated with 10 mM imidazole in the lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... and H2A.Z (fused with a maltose-binding protein -MBP) purification were performed by affinity chromatography using Ni-NTA agarose (Qiagen) and amylose resin (NEB) ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was filtered with a 0.45 μm filter and loaded onto a nickel-charged immobilized metal affinity chromatography column (Qiagen). After washing with ten column volumes of buffer A ...
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bioRxiv - Neuroscience 2020Quote: ... PrP was expressed in Rosetta2(DE3)pLysS E.coli competent cells and purified by affinity chromatography using Ni2+-nitrilotriacetic acid Superflow resin (QIAGEN). In the RT-QuIC assay ...
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bioRxiv - Neuroscience 2020Quote: ... was expressed in Rosetta2(DE3)pLysS E.coli competent cells and purified from inclusion bodies by affinity chromatography using Ni2+-nitrilotriacetic acid Superflow resin (QIAGEN). Recombinant hamster PrP (HaPrP ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed by sonication and ΔN23-Sar1-6xHis was purified by nickel affinity chromatography (Ni-NTA resin, Qiagen) and eluted with elution buffer (40mM Tris pH 8 ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant SlERF.D7-6XHIS protein was purified by Ni2+gravity flow chromatography according to the manufactureer’s protocol (Ni-NTA Agarose, Qiagen) as described ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant RIN-6XHIS protein was purified by Ni2+ gravity flow chromatography according to the manufacturer’s protocol (Ni-NTA Agarose, Qiagen). For the EMSA assay ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction containing the His-VitR protein was purified using NTA-resin affinity chromatography in phosphate buffer according to manufacturer’s recommendations (Qiagen). After concentration (Vivaspin 6 Concentrator ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant protein was purified by affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant protein was purified by affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21 Star (DE3) strain and purified by Ni-NTA affinity chromatography with a Ni-NTA agarose column (QIAGEN), followed by size exclusion chromatography with a HiLoad 26/600 Superdex 75 pg column (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by cell lysis and purification using immobilized metal-affinity chromatography (IMAC) in a Ni–NTA (Qiagen, Valencia, CA) column in binding buffer composed of 20 mM HEPES pH 7.25 ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant proteins were purified to near homogeneity (>95%) using Ni-chelate affinity chromatography on Ni-NTA Superflow resin (Qiagen) using standard protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain (Rosetta) and purified using Ni-NTA affinity chromatography according to the manufacturer’s instructions (Qiagen Cat No./ID: 30210). Purified protein was used as an immunogen to raise polyclonal antibody in rabbits at a local commercial facility (DPL ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21 (DE3) as fusion proteins containing an N-terminal His6- tag and purified by immobilized metal-affinity chromatography (Ni-NTA, Qiagen). Full-length DosR was also expressed with N-terminal GST-tag ...
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bioRxiv - Biophysics 2019Quote: ... The resulting adduct mSav*-cc-PS-CFP2* was purified together with an excess of PS-CFP2*-tetrazine via Ni-NTA based affinity chromatography (QIAGEN) and gel filtration (Superdex 200 10/30 ...
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bioRxiv - Genetics 2020Quote: ... Cell debris was pelleted and the supernatant collected and used for immobilized metal affinity chromatography using Ni-NTA agarose beads (Qiagen) and agitated for at least 2 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). Polyclonal rabbit antisera were raised by Covance Research Products Inc ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns were used for a 2 L culture in which the lysate was loaded on the column washed with 10 CV wash buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns (flow 5 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... Metal-chelate affinity chromatography was used to purify the 6xHis-tagged proteins using the Ni-NTA Fast Start Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the His-ChuP protein was purified from the soluble extract by affinity chromatography in a Ni-NTA Superflow column (Qiagen). The elution fractions were evaluated using 18% SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: His-FlhAC and His-FlgN were purified by Ni affinity chromatography with a nickel-nitriloacetic acid (Ni-NTA) agarose column (QIAGEN) as described previously12 ...
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bioRxiv - Plant Biology 2019Quote: ... C-terminal His-tagged proteins were purified by gravity-flow chromatography on a nickel nitrilotriacetate (Ni-NTA) agarose resin (Qiagen) according to the manufacturer’s recommendations followed by an exclusion chromatography on a Superdex75 column connected to an ÄKTA Purifier™ (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified using Ni-NTA immobilized metal-affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow Resin (GE Lifesciences) or NiNTA Agarose (QIAGEN) and gravity flow ...
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bioRxiv - Genetics 2020Quote: ... The supernatant of the transfected cells encoding for N-HIS-FAM19A5 was collected for Ni-NTA affinity chromatography (Qiagen, Germany). Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific ...