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Citations for Qiagen :
1 - 50 of 227 citations for Ion Exchange Chromatography since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... anion exchange chromatography and by Ni-NTA-Agarose (Qiagen #1018244) affinity chromatography as described previously [2,39–41] ...
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bioRxiv - Biophysics 2021Quote: ... DNA plasmids were purified using anion-exchange gravity flow chromatography (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was collected and purified using Nickel ion affinity chromatography (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The Klenow polymerization reactions were incubated at 37 °C for 1 h and then purified on ion-exchange cartridges (cat. no. 28306; Qiagen). The 194 purified reactions were dissolved in 50 µL of water each and pooled to generate the 3,880 members library (DEL3880 ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were further purified by reverse immobilized metal ion affinity chromatography (IMAC) using TALON resin (Qiagen), to remove uncleaved protein ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting supernatant containing soluble LCC was subjected to purification by immobilized metal ion chromatography (IMAC) using Ni-NTA Superflow (Qiagen, Hilden, Germany). Protein elution using 250 mM imidazole was then dialyzed against 100 mM potassium phosphate buffer (pH 8 ...
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bioRxiv - Physiology 2022Quote: ... Using the Neuronal ion channel plate (Qiagen, UK), 84 ion channels as well as housekeepers were measured in each sample ...
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bioRxiv - Biochemistry 2021Quote: ... Following Ni-NTA affinity chromatography (Qiagen), the NELF containing protein fractions were pooled ...
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bioRxiv - Biochemistry 2020Quote: ... Nickel affinity chromatography resin (NiNTA, Qiagen) was used to purify HNL1 containing a 6-His tag expressed in Escherichia coli ...
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bioRxiv - Biochemistry 2023Quote: ... chromatography columns containing Ni-NTA agarose (Qiagen) were equilibrated in binding buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... chromatography columns containing Ni-NTA agarose (Qiagen) equilibrated in binding buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Purification was performed by nickel affinity chromatography under denaturing conditions using the Ni-NTA Agarose gravity chromatography System (Qiagen). Inclusion bodies containing insoluble recombinant proteins were isolated by successive rounds of sonication and centrifugation ...
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bioRxiv - Molecular Biology 2022Quote: ... A combination of nickel affinity chromatography (Qiagen 30210) and cation exchange (GE Healthcare HiTrap-SP ...
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bioRxiv - Immunology 2022Quote: ... Affinity chromatography with Ni-NTA agarose beads (Qiagen) was used to purify the proteins ...
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bioRxiv - Bioengineering 2024Quote: ... before purification by Ni-NTA affinity chromatography (Qiagen) as previously described (Fierer et al. ...
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bioRxiv - Plant Biology 2020Quote: ... metal chelate affinity chromatography on Ni-NTA Agarose (Qiagen) was performed according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2019Quote: ... The purification protocol entailed Ni-NTA agarose chromatography (Qiagen), Heparin sepharose chromatography (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... and subjected to Ni-NTA based affinity chromatography (QIAGEN). Eluted protein was further purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... followed by affinity chromatography using Ni-NTA resin (QIAGEN) and size exclusion chromatography using a HiLoad Superdex 200 pg column (Cytiva) ...
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bioRxiv - Biochemistry 2020Quote: ... FL-p53 was purified by nickel-NTA chromatography (Qiagen) following manufacturers protocols ...
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bioRxiv - Neuroscience 2020Quote: ... GAPDH protein was purified by Ni-NTA chromatography (Qiagen) under native conditions ...
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bioRxiv - Microbiology 2020Quote: ... coli by affinity chromatography using Ni-NTA agarose (Qiagen) according to the manufacturer’s instructions (Ruc-His6 was purified under urea denaturing conditions) ...
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bioRxiv - Microbiology 2022Quote: ... and purified by metal-affinity chromatography (Ni-NTA, Qiagen). The protein expression was induced by adding 0.4 mM IPTG in log-phase cultures at O.D600 of 0.4 ...
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bioRxiv - Biochemistry 2020Quote: ... and purified by Ni-NTA– agarose affinity chromatography (Qiagen) as described previously (15) ...
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bioRxiv - Microbiology 2021Quote: ... followed by affinity chromatography using Ni-NTA resin (QIAGEN) and size exclusion chromatography using a HiLoad Superdex 200 pg column (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were purified with Ni-column chromatography (Qiagen) by following the manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2023Quote: ... purified by non-denaturing Ni-NTA affinity chromatography (Qiagen), and buffer exchanged into PBS by overnight dialysis through regenerated cellulose tubing (6-8 kDa MWCO ...
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bioRxiv - Biochemistry 2023Quote: ... protein was captured using Ni-NTA affinity chromatography (Qiagen). Protein was further purified using ion exchange chromatography preceding size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... TAT-SAPIP was purified by Nickel affinity chromatography (Qiagen) following the protocols from the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... Ion-chelated IMAC beads were prepared from Ni-NTA Superflow agarose beads (Qiagen, MA). Nickel ion was stripped with 50 mM EDTA and iron was chelated by passing the beads through aqueous solution of 200 mM FeCl3 followed by three times of water wash and one time wash with binding buffer (40% acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was subjected to Ni2+-NTA affinity chromatography (Qiagen) followed by size exclusion chromatography using a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen), the 6 x His-tag was removed via incubation with thrombin protease overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... NT-hFMRP was purified using Ni-NTA affinity chromatography (Qiagen). GST-hFMRP RGG ...
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bioRxiv - Microbiology 2022Quote: ... and His-PutA was purified with Ni-column chromatography (Qiagen). The purified His-PutA protein (50 μg ...
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bioRxiv - Biochemistry 2022Quote: ... It was then purified by Ni-NTA affinity chromatography (Qiagen)(50mM Tris pH 8 ...
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bioRxiv - Immunology 2019Quote: ... Proteins were purified by affinity chromatography on NiNTA agarose (Qiagen), followed by FPLC on MonoQ (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni-NTA affinity chromatography (Qiagen) and eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: ... Immobilized metal affinity chromatography (IMAC) using Ni-NTA matrix (Qiagen) was employed for purification purposes ...
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bioRxiv - Cell Biology 2020Quote: ... 2ml of packed Ni-NTA affinity chromatography resin (Qiagen, USA) was calibrated with 10 volumes of lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant proteins were purified using nickel affinity chromatography (Qiagen). As the final purification step ...
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bioRxiv - Biochemistry 2022Quote: V1ab4 was purified by Ni-NTA-sepharose affinity chromatography (Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Biochemistry 2022Quote: ... Both mseGFP and roGFP2 were purified by NiNTA chromatography (QIAGEN) over a column bed containing 5ml of resin equilibrated with start buffer ...
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bioRxiv - Bioengineering 2023Quote: ... 86 and purified using immobilized metal-affinity chromatography (Qiagen, 30230) and size-exclusion chromatography ...
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bioRxiv - Biophysics 2023Quote: ... then applied to Ni-NTA chromatography (Ni-NTA Superflow, Qiagen). The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 3k-cutoff filter (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... His6-UBXN7 was purified by Ni2+-NTA affinity chromatography (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... is packed onto a propylene chromatography/cartridge column (Qiagen, Germany). Column is washed adequately with water before use and equilibrated with 15CV with lysis buffer ...
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bioRxiv - Pathology 2021Quote: ... Ion Torrent-specific sequencing libraries were generated using the GeneRead L Core Kit (Qiagen, Hilden, Germany) together with IonXpress barcode adaptors (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...