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Citations for Qiagen :
401 - 450 of 1928 citations for Human Chemokine C X C Motif Receptor 6 CXCR6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The reactions were then treated with RNaseI (Thermo) for 60 min at 37°C and cleaned using the MinElute PCR Purification Kit (Qiagen). Subsequently ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was centrifuged for 10 min at 16,200xg 4°C and 600 μL of the supernatant was added to a 50 μL slurry of Ni-NTA Agarose (Qiagen) that had been washed three times with 250 μL A3B-CTD lysis buffer without 2-mercaptoethanol and once with 250 μL of A3B-CTD lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... worms were homogenized in PBS (50 μl/adult worm pair or 50 μl/1000 schistosomula) at 4°C using a TissueLyser II (Qiagen), homogenates were incubated overnight with mixing at 4°C and the supernatants collected by centrifugation at 15,000 g for 1 h at 4°C ...
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bioRxiv - Pathology 2019Quote: ... DNA from D&C and endometrial biopsy specimens was extracted using the QIAamp® DNA FFPE Tissue Kit (QIAGEN, USA). All DNA was quantitated using the Qubit 2.0 Fluorometer (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were incubated for 15 minutes at 37°C to methylate accessible chromatin before the reaction was stopped with the addition of RLT plus buffer (Qiagen) and samples frozen down and stored at -80°C prior to processing ...
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bioRxiv - Genomics 2019Quote: ... Reactions were incubated for 30 min at 37° C in a thermomixer shaking at 300 rpm and DNA purified using Qiagen PCR clean-up MinElute kit (Qiagen). The transposed DNA was subsequently amplified in 50µl reactions with custom primers as described (Buenrostro et al. ...
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bioRxiv - Microbiology 2019Quote: ... an 8 μg of the isolated RNA was further treated with 2U of TURBO DNase (Invitorgen) at 37°C for 1 h and purified with RNeasy spin columns (Qiagen). The amount of RNA was quantified with Qubit RNA BR assay kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... This was followed by RNase A treatment for 1 h at 37°C and purification using MinElute PCR purification columns (Qiagen). cDNA amplification was performed with reporter-specific nested cDNA primers (Oligos 16 and 17 for libraries in vectors d and e ...
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bioRxiv - Genomics 2021Quote: ... To eliminate lipids supernatants were applied to a RNeasy column and centrifuged at 10,000 rpm and 4 °C for 1 min (Qiagen, 74104). The flow through was collected and protein concentrations were assessed using the Bradford assay ...
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bioRxiv - Genetics 2021Quote: ... Swabs were stored without media at - 20°C until DNA was extracted with QiaAmp DNA Mini kit (Qiagen, Germantown, MD). Nasal shedding status of M ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
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bioRxiv - Developmental Biology 2020Quote: ... overnight incubation at 37 °C) and DNA was extracted using the QiaAmp Blood Mini Kit (Qiagen Cat No./ID: 51304) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Confirmation and segregation of the HNRNPUL1 c.1406dup variant was performed by PCR amplification (HotStar Taq Plus, Qiagen, Toronto, ON) from genomic DNA and Sanger sequencing with the ABI BigDye Terminator Cycle Sequencing Kit v1.1 (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2020Quote: ... washed twice with ddH2O and digested at 55°C overnight with 600 μL ATL buffer from DNeasy Blood and Tissue Kit (Qiagen), 24 mAu proteinase K (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 45 min after which 25 µl of proteinase K (20 mg/mL) and 200 µl Buffer AL (Qiagen) was added ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Lysates were cleared by centrifugation at 15000 x g for 30 min at 4 °C and incubated in Pierce 5 ml columns with Ni-NTA agarose beads (Qiagen) at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were clarified by centrifugation at 24,000g at 4°C for 20 min and passed through 2 mL of Ni-NTA nickel resin (Qiagen, 30250) pre-equilibrated with wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cβ primer ...
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bioRxiv - Immunology 2021Quote: ... Cells were rinsed at rested at 37°C for a minimum of 1h before undergoing red-blood-cell lysis by 5-10 ml RBC lysis solution (Qiagen) for 20 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Lysates were clarified by centrifugation at 12,000xg at 4°C and mixed with 200 µL of a slurry of Ni2+-NTA agarose (Qiagen #30210) that had been equilibrated in lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... All plugs were incubated twice (2 hours incubation followed by an overnight incubation) at 50 °C with freshly prepared 167 μl Proteinase K (Qiagen) in 2.5 ml lysis buffer (BioNano Genomics ...
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bioRxiv - Bioengineering 2021Quote: ... was added to the digested vector and the mix was incubated at 37°C for 30 minutes before it was purified with a QIAquick PCR Purification Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen). The gene fragments were ligated into the backbone using T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2020Quote: Tissue samples were preserved in RNA-Later at −80 °C and total RNA was isolated using RNeasy mini kit (QIAGEN) as previously described 55 ...
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bioRxiv - Immunology 2022Quote: ... Kidneys were mechanically disrupted with metal beads during 3 min at 4°C and DNA was then extracted using QIAmp DNA kit (Qiagen). Leptospiral DNA was specifically targeted using primers and probes designed in the lpxA gene (L ...
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bioRxiv - Microbiology 2022Quote: ... DNA on the exterior of filtered capsids was digested for 1 hour at 25 °C with 20.3 units DNase (Qiagen: 79254) supplemented with 10 mM MgCl2 ...
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bioRxiv - Neuroscience 2022Quote: We euthanized groups of 20 to 30 zebrafish larvae by immersion in ice-cold water (below 4°C) and immediately performed RNA extraction using the RNeasy Mini Kit (Qiagen) and reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were snap frozen and stored at-80 °C until being processed for RNA isolation using the RNeasy RNA extraction kit (Qiagen) with DnaseI treatment using RNase-Free DNase (Qiagen) ...
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bioRxiv - Biophysics 2022Quote: ... The liquid culture was then grown for 3 hours at 37°C before the plasmid extraction using a miniprep kit (QIAGEN). Prepared DNA was sequenced by the Microbial Genome Sequencing Center (https://www.seqcenter.com/ ...
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bioRxiv - Cancer Biology 2022Quote: ... The cleaved fragments were released by incubating the tube for 30□min at 37□°C and then purified by Qiagen MinElute PCR Purification Kit ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease73 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was discarded before resuspending the nuclei pellet in 30 mL prewarmed (37 C) G2 lysis buffer (Qiagen, Germany). Resuspended pellets were incubated with RNase A (50 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... A melting curve determination experiment from 20 to 95 °C was then performed on the reaction on a Rotor-GenQ qPCR machine (QIAgen) with 563 excitation filter and 610 high-pass emission filter (gain:10) ...
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bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were induced with 100 μM IPTG at 12°C for 16 hrs and purified on a Ni2+ column according to manufacturer protocols (Qiagen). Proteins were eluted using 125 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... bacteriovorus HID13, and from −80°C frozen Aquifex aeolicus pellets (purchased from the Archaeenzentrum Regensburg, Germany) using the RNEasy kit (Qiagen), including DNase I treatment ...
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bioRxiv - Synthetic Biology 2022Quote: ... were set up on ice avoiding bubbles and incubated at 37 °C for about 25 min in a PCR cycler Rotor-Gene Q (Qiagen, excitation at 470 nm ...
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bioRxiv - Genomics 2022Quote: ... The mixture was incubated at 16°C for 2 h and the resulting double-stranded DNA was purified using the MinElute PCR Purification kit (Qiagen) with 20μl EB for elution ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was mixed for 30 min at 37°C and subjected to purification using the MinElute Reaction Cleanup Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... They were subsequently organized in metal brackets that were stored at -20°C and homogenized with stainless steel beads at 30 Hz using a TissueLyser II (Qiagen). Samples were lysed twice for one minute ...
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bioRxiv - Biophysics 2022Quote: ... The resulting mixture was rotated at 4 °C for 60 min and then poured into a 1 mL polypropylene column (Qiagen). The resin was washed three times with 5 mL lysis/wash buffer and eluted in 0.25 mL fractions with Ni-NTA elution buffer (composition same as lysis/wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... Cell debris was cleared by centrifugation at 40,000 g for 45 min at 4°C and supernatant was applied to His-tag affinity purification using Ni-NTA agarose (beads or 5 ml column, Qiagen) in buffer A (50 mM TRIS ...
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bioRxiv - Cell Biology 2023Quote: ... and the membrane was cut out and submerged in buffer RLT then frozen at −80°C for RNA extraction using RNeasy Kit (cat no. 74104, Qiagen), cDNA synthesis using SuperScript™ III First-Strand Synthesis System (cat no ...
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bioRxiv - Neuroscience 2023Quote: ... Sampled tissue punches were flash frozen on dry ice and store at -80 °C until RNA isolation by RNeasy Plus mini kit (Qiagen). RNA quality checking was completed with the Qubit fluorometer system (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated for 30 min at 4 °C with 5 mL of Ni2+-beads (Qiagen Ni-NTA Superflow) equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: Bacterial strains were grown overnight in broth cultures (37° C, shaking at 200 RPM) and had genomic DNA extracted using a DNeasy Blood and Tissue kit (Qiagen). Genomic DNA was sequenced using the Illumina MiSeq at the Heflin Genomics Core at UAB ...