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Citations for Qiagen :
551 - 600 of 1928 citations for Human Chemokine C X C Motif Receptor 6 CXCR6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... Islets were pelleted and stored at −80°C until DNA extraction using the Blood/Tissue DNeasy kit (Qiagen; Germantown, MD, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic bacterial DNA was isolated from overnight grown cultures (LB, 37°C) by the DNA Extraction kit (DNeasy Kit, Qiagen Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The chromatin fragments were then left to reverse crosslink overnight at 65°C with shaking and the DNA was then purified with the QIAquick PCR purification kit (QIAGEN; 28104), according to manufactures instructions ...
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bioRxiv - Neuroscience 2023Quote: ... After protein digestion (PK Buffer and Proteinase K (20 mg/mL) for 2 hrs at 45°C) DNA was purified with QIAquick PCR Purification Kit (Qiagen, Germany) according to manufacturer instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant was discarded and the pellet was frozen at -20 °C for further extraction with the DNeasy® Plant Mini Kit (Qiagen). Total DNA was eluted in 10 µl of water and concentration was measured using NanoDrop ...
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bioRxiv - Systems Biology 2023Quote: ... Liver samples were stored at -140°C and prepared as follows: total RNA was extracted using miRNeasy kit (Qiagen; Hilden, Germany). Libraries were prepared using 10 ng/μl with Truseq Stranded RNA ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were incubated at 37°C for 30 min and then purified using the MinElute Reaction Cleanup kit (Qiagen Cat #28204) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Single sorted cells (∼50’000 cells) were lysed in buffer RLT and stored at -80°C until starting the RNA isolation following manufacturer instructions (Qiagen, 74034). cDNA reaction was performed using qScript™ cDNA SuperMix (QuantaBio ...
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bioRxiv - Microbiology 2023Quote: ... and incubated at room temperature for 20 min Samples were then frozen at -20°C overnight prior to RNA extraction using the QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen, Cat #28206). Library preparation was performed on purified fragments using Nextera DNA Flex Library Prep Kit (Cat #20018704 ...
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bioRxiv - Immunology 2023Quote: For protein production a pT350 plasmid encoding for the construct of interest with a BiP signal sequence in the N-terminal and a double strep-tag in the C-terminal region was co-transfected with a puromycin resistant plasmid (pCoPuro) (61) with effectene reagent (Qiagen, 301425) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following an incubation at 55°C for 1 hr in 450 µl of lysis buffer with proteinase K (QIAGEN, Hilden, Germany). Each colony was genotyped using twelve microsatellite loci69 ...
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bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown in LB at 37°C with shaking and genomic DNA was isolated using the DNeasy® Tissue Kit (Qiagen) from exponential and stationary phase cultures ...
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bioRxiv - Neuroscience 2023Quote: ... Transposition occurred at 37°C for 30 minutes and transposed DNA was purified using the MinElute PCR Purification Kit (Qiagen, 28004). Indexing libraries using Nextera i5 and i7 indexed amplification primers (Illumina XT index kit ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted from fresh specimens stored at -20° C in 95% ethanol with a Qiagen DNeasy kit (Qiagen, Hilden, Germany) following manufacturer’s protocols modified by the recommendations of Cruaud et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... of T4 ligation reaction mix was added to NEB Stbl cell (C3040) for transformation and grown at 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Developmental Biology 2024Quote: ... two independent limbs were microdissected and snap-frozen at −80°C for subsequent total RNA extraction using the RNEasy Mini Kit (QIAGEN, 74134) following protocol ...
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bioRxiv - Microbiology 2024Quote: ... and 10 zirconium beads were added to approximately 5 mg of intestinal tissue stored at -80 °C in RNA later tissue reagent (Qiagen, Germany). A Tissue lyser (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from 18 ONS cell samples (6 AD patients, 6 MCI and 6 HC) were extracted using Allprep universal kit (Qiagen cat. no. 80224). RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Gold Kit (Illumina 20020598 ...
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bioRxiv - Systems Biology 2020Quote: ... 6×10^6 PBMC from each sample were transferred directly to Quiazol regent (QIAGEN), resuspended and immediately aliquoted and stored at −80°C until RNAseq downstream processing ...
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bioRxiv - Molecular Biology 2020Quote: 6 ml Ni-NTA (QIAGEN) was washed with 5 column volumes of wash buffer 1 (150 mM NaCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... Colonies that grown on the LB-antibiotic plate were picked and inoculated into fresh LB-antibiotic medium at 37 °C to prepare overnight culture for plasmids extraction using QIAprep Spin Miniprep kit (Qiagen, Hilden, Germany). The plasmids were then sent for DNA sequencing (1st BASE ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were placed at 37°C for 30 min and the resulting fragmented DNA was purified using the Minelute Cleanup Kit (Qiagen, Hilden, Germany), with elution performed in 10 µl of the provided elution buffer ...
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bioRxiv - Genomics 2019Quote: ... 4°C) the aqueous phase was mixed with 700 μl 70% ethanol and applied to a Silica column (Qiagen RNAeasy Mini kit). RNA was then purified according to the manufacturers recommendations ...
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bioRxiv - Immunology 2021Quote: Total RNA from D+ and D- allografts or kidneys of MCMV infected- and uninfected-BALB/c mice was isolated and purified using the RNeasy Mini Kit and RNeasy MinElute Cleanup kit (Qiagen, Germantown MD) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA from in vitro conidia and hyphae harvested 3 and 7 days post-inoculation of potato dextrose broth grown in the dark at 24°C were extracted with RNeasy spin columns (QIAGEN, Venlo, Netherlands). Illumina TruSeq RNAseq libraries were prepared according to the manufacturer’s instructions and sequenced on an Illumina HiSeq 2500 sequencer (50 bp single reads) ...
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bioRxiv - Immunology 2021Quote: ... The dried DNA pellet was reconstituted in 50 μL H2O treated with 330 μg/mL of RNase A for 2 hours at 37°C and then recovered using the QIAquick PCR Purification kit (QIAGEN Cat#28104). DNA concentration was determined by Qubit (Thermofisher).
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bioRxiv - Plant Biology 2020Quote: ... Plant material was dried at 60°C overnight and ground to powder using a ball mill (Tissue Lyser II, Qiagen, Venlo, Netherlands). Aliquots of about 1.5-2 mg were transferred into tin capsules (IVA Analysentechnik ...
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bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
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bioRxiv - Microbiology 2022Quote: ... aeruginosa cells were lysed after 18 hours using 2.5mg/mL lysozyme and incubated at 37°C for 30 minutes and RNA was extracted using the RNeasy mini kit (QIAGEN, Germantown, MD). DNase treatment was done twice for better DNA removal using Qiagen RNase-free DNase during RNA extraction and using NEB DNase after RNA extraction ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 12-d-old seedlings grown at 22°C in LD conditions at ZT13 with the RNeasy® Plant Mini Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...
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bioRxiv - Developmental Biology 2022Quote: ... was linearized with BbsI-HF restriction enzyme for 1 hour at 37°C and gel purified with QIAquick® Gel Extraction Kit (Qiagen, 28704). Two gRNAs targeting ITGBβ1 (Horlbeck et al. ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: Cells were lysed in RLT PLUS buffer and stored at -80°C until RNA isolation with the RNeasy Micro Kit (Qiagen, Hilden, Germany). cDNA was generated using the iScript Advanced cDNA generation kit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... and then amplified using 40 cycles of 95°C for 3 s and 60°C for 30 s using either the Rotor-Gene Q or Rotor-Gene 6000 platform (Qiagen, Hilden, Germany).
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bioRxiv - Evolutionary Biology 2019Quote: Total DNA was extracted from tadpole tails by overnight digestion in 10% proteinase K solution at 56 °C and extracted using the Qiagen Biosprint 96 DNA blood kit (Qiagen, CA, USA). DNA was eluted in 100 - 200 μL buffer AE (QIAgen).
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bioRxiv - Biochemistry 2020Quote: ... at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA, Qiagen, Hilden, Germany) previously equilibrated with the corresponding lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... step for 15 min at 4°C to remove insolubilized material and the supernatant was incubated with Ni-NTA agarose beads (Qiagen, cat: 30230) for 2 hrs ...
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bioRxiv - Biochemistry 2020Quote: Total RNAs were extracted from Wildtype MIN6 and MIN6 stably expressing shRNAs (non-specific/NS, C, D) using RNeasy kit (QIAGEN, Germantown, MD) and cDNAs were prepared using qScript cDNA SuperMix (Quantabio ...
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bioRxiv - Evolutionary Biology 2021Quote: Whole bodies of individual male flies were removed from the -80°C freezer and immediately homogenized in Qiazol lysis reagent (Qiagen, Hilden, Germany). The homogenate was treated with DNase I followed by addition of chloroform ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
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bioRxiv - Zoology 2021Quote: ... dead mites were pierced with an 18-gauge needle under a dissecting microscope and digested overnight in lysis buffer and proteinase K at 56 °C (Qiagen, Valencia, CA). Final DNA product from each mite was eluted in 60 µL of AE buffer ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was cleared by centrifugation at 18000 rpm at 4°C and the supernatant was loaded onto 5 mL Ni–NTA resin (Qiagen, Milan, Italy) equilibrated with binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...