Labshake search
Citations for Qiagen :
351 - 400 of 418 citations for Hrms Rapid Pcb Screening Calibration Solution Cs0.05 Unlabeled 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... The beetle microbiomes were extracted by crushing individual whole beetles with a micropestle in 450 µL of MBL solution from the QIAamp BiOstic Bacteremia DNA kit by Qiagen© (one beetle per tube) ...
-
bioRxiv - Cancer Biology 2021Quote: ... the transposed DNA fragments were extracted from the reaction solution using the Mini Elute PCR purification kit (Qiagen Cat# 28004) and then amplified by a 12-cycle PCR amplification step with specific primers as described by Buenrostro et al. ...
-
The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... The retrieved tissue mROIs were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
-
bioRxiv - Cell Biology 2022Quote: RNA was isolated from bone marrow cells using the gDNA eliminator solution for purification of total RNA (RNeasy Plus Mini Kit, Qiagen). cDNA was synthesized by using AMV reverse transcriptase with random primers (Promega) ...
-
bioRxiv - Bioengineering 2022Quote: ... A high salt solution in combination with isopropanol was utilized for precipitation of RNA before purification with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... DRG neurons were transfected in serum free DMEM solution with predesigned fluorescently labelled siRNA directly against mouse TMEM120A (TACAN) from Qiagen-FlexiTube (cat ...
-
bioRxiv - Bioengineering 2021Quote: ... The histidine-tagged TEV and MBP were extracted from the solution using a Ni-NTA agarose resin (Qiagen, Hilden, Germany) as described in[20].
-
bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
-
bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc ...
-
bioRxiv - Genomics 2020Quote: 3 million cells from cell lines or patient samples were pelleted and resuspended in 1 mL of Cell Lysis Solution (Qiagen) mixed with 500 μg of RNase A ...
-
bioRxiv - Genomics 2020Quote: ... The ethanol was then poured out and the pellet was air-dried for 15 minutes before resuspension in 50 to 100 μL of DNA Hydration Solution (Qiagen). DNA was sequenced with paired end Illumina sequencing at 30x coverage.
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue microregions were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
-
bioRxiv - Biochemistry 2021Quote: ... before extraction solution was added to achieve a concentration of 20 mg/mL before immediate lysis in a benchtop TissueLyser LT (Qiagen) operated at 50 Hz for 3 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Cells were rinsed at rested at 37°C for a minimum of 1h before undergoing red-blood-cell lysis by 5-10 ml RBC lysis solution (Qiagen) for 20 min at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA samples were obtained from dissected tissues from adults or from a pool of embryos or larvae using RNAble solution (Eurobio) and a TissueLyser II system (Qiagen). Total RNA samples were treated with DNAse I (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... grown up at 20 °C were washed off from NGM plates using M9 solution and subjected to total RNA extraction using the RNeasy Mini Kit from Qiagen. Purification of poly-A containing RNA molecules ...
-
bioRxiv - Cell Biology 2022Quote: ... animals were maintained at 20 °C and washed down from NGM plates using M9 solution and subjected to RNA extraction using TissueDisruptor and the RNeasy Mini Kit from Qiagen. RNA preparations were used for qRT-PCR or RNAseq ...
-
bioRxiv - Developmental Biology 2022Quote: ... the animals were washed down from NGM plates using M9 solution and subjected to RNA extraction using the RNeasy Mini Kit from Qiagen. 1 μg total RNA from each sample was used for sequencing library construction ...
-
bioRxiv - Genetics 2022Quote: Minced tissues were homogenized with Sepasol RNAI solution (Nacalai Tesque, Kyoto, Japan) using a TissueLyser LT instrument (Qiagen, Hilden, Germany) set at 50 strokes/s for 5 min ...
-
bioRxiv - Genetics 2022Quote: ... were maintained at 20 °C and washed down from NGM plates using M9 solution and subjected to RNA extraction using the RNeasy Mini Kit from Qiagen. RNA-seq library preparation and data analysis were performed as previously described 46 ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
-
bioRxiv - Molecular Biology 2023Quote: ... and sperm using the Bio Basic EZ-10 Spin Column Genomic Minipreps kit for animal sample according to the manufacturer’s protocol except for sperm samples for which the kit ACL lysis solution was replaced by 300 μl of RTL lysis buffer (Qiagen) and 3 μl of mercaptoethanol ...
-
bioRxiv - Genetics 2023Quote: ... were homogenized in G2 solution from NucleoBond buffer kit by a stainless steel bead via the mechanical disruption of TissueLyzer (QIAGEN) for 10min (50 Hz) ...
-
bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... 40 PGs from WPP animals were dissected under ice-cold phosphate-buffered saline (PBS) and stored in RNAlater solution (Qiagen). Total RNA was extracted using Trizol reagent (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... Crystals were grown at 30°C by the hanging drop vapor diffusion method using 2 μL sample drops and 300 μL crystallization solution in a sealed chamber (EasyXtal 15-Well Tool, Qiagen). Crystals were soaked for 1h (for the Na structure or with the dibromo Intronistat B derivative ...
-
bioRxiv - Molecular Biology 2023Quote: ... expanded and initially screened for correct integration using homology- arm spanning PCRs (Immolase DNA polymerase, Bioline, supplemented with Q solution, Qiagen). Genotypes of positive clones were confirmed by Sanger sequencing ...
-
bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were incubated with a PCR mix containing (100 µM dNTP, 1X thermopol Taq reaction buffer, 1X of Q solution (Qiagen) and 1 µM of each primer) ...
-
bioRxiv - Cell Biology 2024Quote: The RNA was extracted from the sorted HSCs using the Trizol-chloroform method followed by DNase treatment in the solution and cleaned up with an RNeasy MinElute clean-up kit from Qiagen. The purity of RNA was confirmed on the Bioanalyzer before proceeding with the sequencing ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were directly lysed in the well using 350 µL of lysing solution (1% 2-mercaptoethanol in Buffer RLT; RNeasy Mini Kit, 74106, Qiagen) by pipetting over the well ...
-
bioRxiv - Cancer Biology 2024Quote: RNA was isolated from 10-μm FFPE samples from resected canine primary melanoma tumor or metastatic tumor lesions using an RNEasy FFPE Kit and deparaffinization solution (Qiagen). Isolated RNA was examined by Bioanalyzer (Agilent ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total DNAs were extracted from the aqueous phase/ethanol solutions according to the manufacturer’s protocol using the DNAEasy Blood and Tissue Kit (Qiagen, Germantown, MD). Total DNA concentrations and purities were measured using NanoDrop (Thermo Fischer ...
-
bioRxiv - Genomics 2020Quote: ... pupae were immediately transferred from −80° C into a 50mL conical tube containing pre-prepared lysis solution consisting of 9.5mL Buffer G2 and 19μL RNase A (Qiagen Cat No. 19101). The pupae were then homogenized using a Dremel motorized homogenizer for approximately 30 seconds on the lowest speed ...
-
bioRxiv - Genetics 2019Quote: ... The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s ...
-
bioRxiv - Systems Biology 2019Quote: ... For improving the lysis step proteinase k and lysozyme were added to the lysis solution and the samples were homogenized using Tissue lyser II (Qiagen S.r.l.). The concentration and purity of RNA were analyzed using a NanoDrop ND-1000 (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... A pellet of 200-400 µl of animals was resuspended in 400 µl Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with 5 µl proteinase K (20 mg/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissue lysis was performed by re-suspending ∼30mg of sliced frozen tissue in a solution containing 1% β-mercaptoethanol in RLT buffer (supplemented with antifoam agent; ID 19088, Qiagen). Next ...
-
bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
-
bioRxiv - Molecular Biology 2022Quote: Nunc MaxiSorp™ 96-well plates were coated with 75 ng/well of penta-His antibody in PBS solution (Qiagen, 34660) and incubated overnight at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... Stigma tissues were removed from tube and DNA was extracted from the solution using DNeasy PowerSoil Pro Kit (Qiagen, Hilden Germany) following manufacturer’s instructions except the following modifications ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted after disrupting the frozen leaves with metal beads in RLT and β mercaptoethanol solution using a Qiagen RNeasy plant mini kit (Qiagen) according to manufacturer’s instructions (without the optional DNase step) ...
-
bioRxiv - Neuroscience 2024Quote: Sorted cells were collected in a lysis-buffer solution supplemented with β-mercaptoethanol and RNA extraction was performed immediately using a standard protocol (RNeasy Plus Micro Kit; Qiagen). The yield and quality of the product were measured with a bioanalyzer (Agilent 2100) ...
-
bioRxiv - Microbiology 2023Quote: ... cells were collected by centrifugation and quickly washed with ice-cold water to remove the methanol and resuspended in RNAlater solution (Qiagen, 76104) for at least 2 hours ...
-
bioRxiv - Developmental Biology 2021Quote: ... Every other hour ~300 embryos were counted and lysed in 350μl of a solution of RLT buffer and β-mercaptoethanol from the Qiagen RNeasy Micro Kit (Qiagen, Hilden, Germany). The lysates were immediately stored at −80°C until use ...
-
bioRxiv - Epidemiology 2019Quote: ... spun at 4500g for 12 min and the pellet was re-suspended in 300uL of Dneasy UltraClean Microbial kit Microbead solution (Qiagen 12224-50). The extracted DNA was then prepared for sequencing on an Illumina MiSeq instrument using the NexteraXT kit and sample-specific barcoding ...
-
bioRxiv - Bioengineering 2021Quote: ... the mEV solution was incubated with 100 µl of precipitation buffer B from the Qiagen miRCURY Exosome Isolation Kit (Qiagen, Germantown, MD) overnight (∼12 hours ...
-
bioRxiv - Cancer Biology 2022Quote: ... and areas containing >30% tumor cells as determined by an expert pathologist were macrodissected and deparaffinated using Deparaffinization Solution (Qiagen, Hilden, Germany). DNA was extracted and purified with GeneRead DNA FFPE Kit (Qiagen ...