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Citations for Qiagen :
1 - 50 of 418 citations for Hrms Rapid Pcb Screening Calibration Solution Cs0.05 Unlabeled 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... commercial screening solutions (Emerald Biosystems, Hampton Research, and Qiagen), and the sitting-drop vapor-diffusion technique (drop ...
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bioRxiv - Microbiology 2019Quote: Each HRM reaction contained 12.5 μl Type-it® HRM buffer (Qiagen, UK), primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Developmental Biology 2020Quote: ... EvaGreen (Type-it HRM PCR Kit, Qiagen) and the manufacturers recommended protocol for use ...
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bioRxiv - Molecular Biology 2023Quote: ... EvaGreen (Type-it HRM PCR Kit, Qiagen) and the manufacturers recommended protocol for use ...
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bioRxiv - Physiology 2023Quote: ... The Rotor-Gene Q 5plex HRM System (QIAGEN) was used to perform quantitative real-time PCR ...
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bioRxiv - Microbiology 2021Quote: ... was done using conventional PCR followed by high resolution melting (PCR - HRM) analysis in a HRM capable RotorGene Q thermocycler (Qiagen, Hannover, Germany). Screening for Anaplasma spp ...
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bioRxiv - Biochemistry 2020Quote: ... The initial screening was performed using commercially available screening kits from Qiagen and Molecular Dimensions in 96-well plate format using sitting-drop technique ...
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bioRxiv - Neuroscience 2020Quote: ... The rotor-gene Q 5plex HRM platform (Qiagen, Hilden, Germany) was used for the qRT-PCR ...
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bioRxiv - Plant Biology 2023Quote: ... on the Rotor-Gene Q 5plex HRM Platform (QIAGEN, Germany). Melting curve analysis was performed to confirm the specificity of the product for each primer pair ...
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bioRxiv - Neuroscience 2021Quote: ... The titer of the individual lentiviral solution was quantified by Lenti-X p24 Rapid Titer Kit (Qiagen, 632200) at the first round of production and then Lenti-X GoStix Plus Kit (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... Positive HRM samples were isolated using a PCR purification kit (Qiagen) and submitted to the University of Guelph ...
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bioRxiv - Cancer Biology 2022Quote: ... 99% Buffer TCL (Qiagen, cat# 1031576), sealed with perfluorinated oil (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... screening PACT Suite and JCSG Suite (Qiagen), JBScreen Classic 1-4 and 6 (Jena Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... screening PACT Suite and JCSG Suite (Qiagen), JBScreen Classic 1-4 and 6 (Jena Bioscience ...
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bioRxiv - Molecular Biology 2019Quote: ... PCRs were performed in an HRM-capable RotorGene Q thermocycler (Qiagen, Hilden, Germany). Every run had a set of known positive control samples as well as a no-template negative control ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were run on a Rotor-Gene Q 5plex HRM System (QIAGEN) for 40 cycles x (95 °C/10s ...
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bioRxiv - Biophysics 2023Quote: ... Melting curves were recorded in a Rotor-Gene Q 5Plex HRM system (Qiagen) using a temperature range of 25-85°C with 1°C steps each 30 s and 90 s prewarm ...
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bioRxiv - Immunology 2020Quote: Initial crystallization hits were obtained via sparse matrix screening in 96-well plates using commercially available crystallographic solutions (Qiagen, Toronto, ON). Optimization of crystallization conditions was performed in 24-well plate format using the hanging drop vapor diffusion method ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR-HRM analyses were carried out in a RotorGene Q thermocycler (Qiagen, Germany) as described by Ouso et al ...
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed with a Rotor-Gene Q 2plex HRM thermocycler (Qiagen) using an SYBR Green reagent (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplification was performed using a Rotor-Gene Q MDx 2plex HRM (Qiagen, Hilden, Germany) for 45 cycles (95°C for 15 s ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cynomolgi detection was performed by using Rotor Gene Q 5plex HRM platform (Qiagen, Hilden, Germany). Primer and probe were designed to target P ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression analysis was carried out in the Rotor-Gene Q 5plex HRM System (QIAGEN) using the DyNamo Colorflash SYBR Green kit ...
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bioRxiv - Cancer Biology 2022Quote: ... The real-time PCR reactions were performed using the Rotor-Gene Q5plex HRM System (Qiagen). The relative quantification of the mRNA fold change was calculated as 2-ΔΔCt and was expressed normalized with endogenous control β-actin ...
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bioRxiv - Microbiology 2021Quote: ... for screenings or exoEasy Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2019Quote: Real-time quantitative PCR (qPCR) was performed on a Rotor-Gene Q 2plex HRM Platform (Qiagen) using SYBR Green as the detection dye ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using a Rotor-Gene Q 5plex HRM Platform (Qiagen). All reactions were performed on at least three biological and three technical replicates ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using a Rotor-Gene Q 5plex HRM Platform (Qiagen). Each reaction was carried out in a total volume of 15 μL containing 2 μL of diluted cDNA (about 10 ng) ...
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bioRxiv - Developmental Biology 2024Quote: ... run by QIAxcel DNA Screening Kit (QIAGEN, Cat. No. 929004), detected by High Performance Nucleic Acid Analyzer (eGENE HDA-GT12 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed with rapid RNA extraction using a RNeasy Kit (Qiagen). cDNA was prepared from 500 ng total RNA with the SensiFAST cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Zoology 2020Quote: ... Representative positive amplicons from PCR-HRM amplification were purified using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions and sequenced.
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bioRxiv - Zoology 2020Quote: ... The PCR products were analyzed with the QIAxcel DNA Screening Kit (Qiagen) to confirm successful amplification ...
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bioRxiv - Biochemistry 2020Quote: ... Crystallization screening was performed at 4°C with JCSG core I (Qiagen) using the sitting drop vapor diffusion method ...
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bioRxiv - Plant Biology 2023Quote: ... and 20 ng cDNA were subjected to the Rotor-Gene Q 5-Plex HRM real-time PCR system (Qiagen), using the default program ...
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bioRxiv - Microbiology 2021Quote: ... and a high resolution melt curve (HRM) in the end] using the primers listed in Table S2 on a Rotor-Gene Q instrument (Qiagen). rnpB was taken as reference for all genes ...
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bioRxiv - Plant Biology 2023Quote: Detection of SNPs as genetic markers were performed by high-resolution melt analysis (HRM) by using the Rotor Gene Technology (Qiagen). PCR was carried out in 20 µl volume containing 20 ng template DNA ...
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bioRxiv - Biochemistry 2020Quote: ... 14 and 22 mg/mL for initial screening using commercially available screens (Qiagen) by sitting-drop vapor diffusion using a Crystal Gryphon robot (Art Robbins Instruments) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 %(w/v) PEG 2000 MME from PEGs I suite screening plate (Qiagen). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... The procedure entailed touch-down PCR amplifications followed by melting of the amplicons in an HRM capable thermal cycler (Qiagen, Germany). The assays were performed in 10-μl reaction volumes ...
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bioRxiv - Biochemistry 2020Quote: Purified CrTRXz was tested for crystallization on commercial sparse-screening conditions (Qiagen, Hilden Germany) of the Joint Center for Structural Genomics screens [97] with a mixture of 30 nL protein and 30 nL precipitant equilibrated against a reservoir volume of 30 µL mother liquor ...
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bioRxiv - Bioengineering 2020Quote: ... Crystallization screening was performed in multiple 96-condition spare matrix suites available from Qiagen or Hampton Research ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were separated in QIAxcell Advanced using the QX DNA Screening Kit (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... and amplicons were separated in QIAxcell Advanced using the QX DNA Screening Kit (Qiagen). The primers used for the MLST study are listed on Supplementary Table S2 ...
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bioRxiv - Biochemistry 2021Quote: ... Initial conditions were identified in 96-well sparse matrix screening trays (Qiagen and Hampton Research) dispensed by an Art Robbins Instruments Phoenix liquid-handling robot ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were visualised using a QIAxcel DNA screening kit in a QIAxcel instrument (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... The autophagy screening was performed using the RT2 Profiler™ PCR Array Human Autophagy (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... This was preferred over rapid gDNA isolation kits such as the DNeasy Blood & Tissue kit (Qiagen), as the latter isolation kit is strongly biased towards shorter DNA fragments (predominantly 30kb in length compared to up to 150kb for the 100/G columns ...
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bioRxiv - Biochemistry 2019Quote: Screening of crystallization conditions was performed using a Mosquito drop dispensing automate (TTPLabtech) and crystallization screens (Qiagen). Drops were prepared by mixing 150 nL of protein solution with an equivalent volume of screening solution ...
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bioRxiv - Microbiology 2020Quote: ... total genomic DNAs of different samples were extracted by using the QIAamp Rapid DNA Kit (Qiagen, Germany). The DNA concentration and integrity were measured by a NanoDrop2000 spectrophotometer (Thermo Fisher Scientific ...