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Citations for Qiagen :
151 - 200 of 1598 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Maize and soybean leaf and root tissue were pulverized for 2-min at a speed of 30 Hz with two 4-mm stainless balls in a TissueLyser II (Qiagen, Venlo, Netherlands). Total DNA was extracted from plant tissues with the OMEGA Mag-Bind Plant DNA Plus kit (Omega Bio-Tek ...
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bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 100,000 g (31,000 RPM) for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen, Germantown, MD) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from pure overnight cultures of single bacterial colonies using a Qiagen DNeasy Tissue Kit according to manufacturer’s instructions (Qiagen, Germantown, MD). Illumina library construction and sequencing were conducted using the Illumina Nextera DNA Sample Prep Kit with 150bp paired-end reads ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from pure overnight cultures of single bacterial colonies using a Qiagen DNeasy Tissue Kit according to the manufacturer’s instructions (Qiagen, Germantown, MD). Illumina library construction and sequencing were conducted using an Illumina Nextera DNA Sample Prep Kit with 150bp paired-end reads ...
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bioRxiv - Microbiology 2022Quote: The isolates were recovered overnight on blood agar and genomic DNA was extracted from pure cultures using QIAamp DNA mini kit (Qiagen, Germany) following the manufacturers’ instruction ...
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bioRxiv - Cell Biology 2020Quote: ... 350μL RLT buffer supplemented with 1% pure β-mercaptoethanol were added and the cell slurry was transferred onto a QIAshredder spin column (Qiagen). Subsequently ...
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bioRxiv - Pathology 2019Quote: ... The bacterial DNA was extracted from 700 µl of pure culture using the Gentra Puregene Yeast/Bact Kit (Qiagen, The Netherlands). DNA (about 40 ng/µl ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial genomic DNA was isolated from pure culture using QIAGEN Genomic-tip 100/G following the manufacturer’s instruction of (Qiagen, Valencia, CA). The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24 ...
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bioRxiv - Plant Biology 2021Quote: Photosynthetic pigments were extracted with 95% ethanol after freezing shoot apices in liquid N2 and grounding them in a TissueLyser (Qiagen, Hilden, Germany). Chlorophylls a and b were quantified by spectrophotometry (Sumanta et al. ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then purified using Qiagen MinElute columns and eluted in 16 µl EB (Qiagen). 14 cycles of PCR were performed on 5 µl of the immunoprecipitated DNA using the single-end Illumina PCR primers ...
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bioRxiv - Systems Biology 2022Quote: ... ribosomal RNA (rRNA) was removed using the QIAseq FastSelect −5S/16S/23S Kit (335925; Qiagen) and stranded mRNA libraries were prepared using the QIAseq Stranded RNA Lib Kit (180743 ...
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bioRxiv - Microbiology 2022Quote: ... the DNA extraction negative control and the QIAseq 16S/ITS Smart Control (Qiagen, Hilden, Germany), a synthetic DNA sample used both as positive control for library preparation and sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the QIAseq FastSelect 5S/16S/23S kit for bacteria (Qiagen), according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... RNA extractions substituted lysis buffer containing proteinase K solution in PKD buffer (1:16) (Qiagen) for the Mag-Bind beads used in the manufacturer protocol.
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bioRxiv - Bioengineering 2024Quote: ... ribosomal RNA (rRNA) was removed using the QIAseq FastSelect –5S/16S/23S Kit (335925; Qiagen) and stranded mRNA libraries were prepared using the QIAseq Stranded RNA Lib Kit (180743 ...
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bioRxiv - Evolutionary Biology 2023Quote: Depletion of rRNA from extracted RNA was performed using QIAseq FastSelect −5S/16S/23S (Qiagen). Samples were incubated at 89°C for 8 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The amplified 16S rRNA gene was purified using a QIAquick purification kit (Qiagen, Hilden, Germany) and amplicons confirmed via gel electrophoresis.
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bioRxiv - Developmental Biology 2021Quote: Neural crest explants from pre-migratory stages (17–18) were collected and processed for RNA extraction using the RNeasy Mini kit (Qiagen, 74104). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was extracted from MIEV-or PKCα-KR-transduced cells from day 17-23 co-cultures using RNeasy mini kit (Qiagen, UK) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Cellular total RNA was prepared from 2-4×106 cells for each sample using the RNeasy Plus Micro kit (Qiagen, Hilden Germany, #74034). For qRT-pCR ...
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bioRxiv - Genomics 2021Quote: ... Quantification of ACE2 transcript levels was performed by preparing total RNA from 2-4×106 cells for each sample using the RNeasy Plus Micro kit (Qiagen, Hilden Germany, #74034). For qRT-pCR ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from serially diluted and the 4 different media SARS-CoV-2 samples using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Three technical replicates of each biological sample were analysed using a Rotor-Gene Q 2plex (Qiagen, Rotor-Gene-Q Pure Detection Software). Control reactions without cDNA template (qPCR grade water used instead ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR sample was then digested with DpnI to cut the residual template DNA and purified with the Pure Link PCR purification kit (Qiagen, Hilden, Germany). The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit ...
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bioRxiv - Microbiology 2024Quote: ... The PCR sample was then digested with DpnI to cut the residual template DNA and purified with the Pure Link PCR purification kit (Qiagen, Hilden, Germany). The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit ...
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bioRxiv - Microbiology 2024Quote: ... The PCR sample was then digested with DpnI to cut the residual template DNA and purified with the Pure Link PCR purification kit (Qiagen, Hilden, Germany). The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation ...
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bioRxiv - Immunology 2021Quote: ... Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C, 30 min; 95°C, 5 min) using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA). cDNA was then amplified using a SYBR Green I Master mix (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... 16 mg of total cell extract (TCE) were incubated with 320 μL of NiNTA beads (Qiagen) at 4 °C ...
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bioRxiv - Microbiology 2019Quote: The gyrB and 16S rRNA gene amplicons were purified using a QIAquick kit (Qiagen, Hilden, Germany) and their quality and concentration were checked with a DNA1000 chip (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... After 16-24h (~ 70% confluency) cells were transfected with 300ng of plasmid mix using Effectene (Qiagen) and following manufacturer instructions for adherent cell cultures ...
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bioRxiv - Microbiology 2021Quote: ... 16S rRNA gene amplicons were cleansed using a QIAquick® PCR Purification Kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2022Quote: DNA extraction for 16S rRNA gene amplification was performed using the PowerSoil DNA Isolation Kit (Qiagen). Total DNA for metagenome sequencing was obtained using an Advanced Soil DNA Kit (mCHIP) ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated on day 12-16 by using the DNeasy Blood and Tissue Kit (Qiagen). The FKBP12 site was amplified with the PlatinumTM SuperFi DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Colonies were cultured for 16 hr at 37°C and DNA was prepared by maxiprep (Qiagen). The entire homology arm sequence and the whole assembled sequence was verified (Genewiz).
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bioRxiv - Systems Biology 2024Quote: ... Whereas 26 fin and 16 kidney samples were extracted using the DNeasy Blood & Tissue Kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and a CTAB-based DNA extraction method (17 months sample) (24) or the DNeasy Blood & Tissue Kit (39 months sample; Qiagen, Hilden, Germany). Concentration and quality of the obtained DNA was checked using a Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: MIEV-or PKCα-KR-transduced HSPCs were co-cultured on OP9 cells in the presence of IL-7 for 17-23 days (late co-culture) and total RNA was isolated using an RNeasy kit (Qiagen, Manchester, UK) from five independent co-cultures ...
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bioRxiv - Cell Biology 2023Quote: ... and siNET1 #4 (Qiagen cat# SI00082040 ...
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bioRxiv - Microbiology 2020Quote: ... were amplified from total RNA extracted from each culture supernatant (MagNA Pure 96 system; Roche Life Science, Superscript III reverse transcriptase; Invitrogen, HotStar Taq DNA polymerase; Qiagen, Venlo, The Netherlands). Fragmented and barcoded DNA libraries were generated (Nextera XT Library Prep Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Networks and upstream regulator analysis were conducted by Ingenuity Pathway Analysis (IPA®; version 01-16, Qiagen). The BAM files with alignments and analysis results of mRNA-seq data have been deposited to Gene Expression Omnibus (GEO ...
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bioRxiv - Cell Biology 2020Quote: ... DEN15d respectively compared with Non-DEN were subjected to Ingenuity Pathway Analysis (IPA) (QIAGEN, version 01-16) for “Core Analysis” and further “Comparison Analysis” ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA from 16 tick pools was then extracted using the RNeasy® Plus Universal kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... rRNA was depleted using the QIAseq FastSelect rRNA HMR and 5S/16S/23S kits (QIAGEN, Hilden, Germany), and libraries were paired-end sequenced (150 bp ...
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bioRxiv - Genetics 2024Quote: ... and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen, Hilden Germany) in combination ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were weighed and homogenized in 4 volumes of water (4 µL of water/mg tissue, 4°C) using a bead beater (TissueLyser II, QIAGEN; Germantown, MD). Aqueous homogenates were profiled using four complimentary liquid chromatography tandem mass spectrometry (LC-MS ...
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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Building a robust chromatin immunoprecipitation (ChIP) method with substantially improved efficiencybioRxiv - Plant Biology 2020Quote: ... DNA purification: (16) The reverse crosslinked samples were purified using a DNA purification spin column (Qiagen, Hilden, Germany), and the column was eluted twice with 60 µl of TE buffer ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted at 16-24 hours post infection by the DNA Easy Blood and Tissue Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... All 16S rRNA gene amplicons were purified from 1.5 % agarose gels using the QIAquick Gel Extraction Kit (Qiagen), quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: Twenty-four multi-amplicon libraries were prepared using the QIAseq 16S/ITS Screening Panel kit (Qiagen, Hilden, Germany) as detailed below ...