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Citations for Qiagen :
1 - 50 of 1598 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... thymine-d4 and glycocholate-d4 internal standards (Cambridge Isotope Laboratories. The cell pellets were homogenized using the QIAGEN TissueLyser II with 3mm Tungsten beads for 4 min at a frequency of 20 Hz ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from d2 (n. 2 biological replicates) and d4 (n. 2 biological replicates) cells with QIAzol lysis reagent (Qiagen #79306), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was extracted as previously described (16, 17) with the DNAeasy PowerSoil DNA extraction kit (QIAGEN).
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bioRxiv - Genetics 2024Quote: ... PCR was performed with 2 µL (∼16 ng) or 4 µL (∼32 ng) of BT-converted DNA with HotStar Taq Polymerase (Qiagen), depending on the genomic region ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from pure cultures using a DNeasy UltraClean 96 Microbial Kit (Qiagen 10196-4) or DNeasy Blood and Tissue Kit (Qiagen 69504 ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from pure cultures using a DNeasy UltraClean 96 Microbial Kit (Qiagen 10196-4) or DNeasy Blood and Tissue Kit (Qiagen 69504 ...
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bioRxiv - Systems Biology 2023Quote: ... GeneRead Pure mRNA Kit (Qiagen) was then used to isolate mRNA from the total RNA for Illumina NGS sequencing according to manufacturer’s specification.
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bioRxiv - Systems Biology 2023Quote: ... GeneRead Pure mRNA Kit (Qiagen) was then used to isolate mRNA for Illumina NGS sequencing from the RNA according to manufacture’s specification.
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bioRxiv - Neuroscience 2023Quote: ... samples obtained by FACS together with 100 ng carrier RNA (PolyA- and PolyA+ mixed in 98:2 ratio) were lysed by Buffer RLT Plus (Qiagen, USA). Then we used PolyA mRNA Magnetic Isolation Module (NEBNext ...
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bioRxiv - Microbiology 2023Quote: ... subtilis 3610 genome (CP020102) (98) using CLC Genomics Workbench software (Qiagen). The enrichment at ribosomal RNA locations were eliminated and the number of reads mapped to each base pair in the genome was exported into a .csv file ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was monitored on Rotor-Gene® Q-Pure Detection System (Software Ver. 2, Qiagen Inc., Valencia, CA, USA) and performed using QuantiFast SYBR® Green PCR Master Mix (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... coli grown for 16 h in 250 ml of 2×YT medium using the Plasmid Maxi Kit (QIAGEN) in accordance with the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was isolated from 16 placentas (3-4 placentas/fetal sex/group) using an RNAeasy kit (Qiagen, Venlo, The Netherlands). Isolated RNA was stored at −80 °C in nuclease-free water ...
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bioRxiv - Developmental Biology 2022Quote: ... 14 and 17 after RA treatment using QIAshredder (Qiagen, 79654) and RNeasy (Qiagen ...
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bioRxiv - Systems Biology 2019Quote: RNA was extracted from a total of 17 samples from Experiment 2 with the AllPrep PowerFecal DNA/RNA Kit (Qiagen USA, Cat. No. 80244). The 17 samples included 7 target samples taken during antibiotic treatment (4 untreated and 3 non-responder ...
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The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... The retrieved tissue mROIs were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue microregions were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
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bioRxiv - Genomics 2022Quote: ... The mixture was incubated at 16°C for 2 h and the resulting double-stranded DNA was purified using the MinElute PCR Purification kit (Qiagen) with 20μl EB for elution ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... pure cultures of cells from cryo-storage underwent DNA extraction (Qiagen) and were submitted for WGS which confirmed that no additional mutations had accumulated in the target strains during mutant construction.
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bioRxiv - Neuroscience 2019Quote: ... Highly pure RNA was isolated using an RNeasy purification kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and the plasmids were purified using Ultra-Pure Miniprep kits (Qiagen) for use in transfections.
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bioRxiv - Neuroscience 2022Quote: ... using a TissueLyser (2 × 30 Hz, 3 min at 4°C; QIAGEN). After homogenization ...
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bioRxiv - Cell Biology 2023Quote: ... elastase (3.125 U/mL, Serva) and DNAse (17 U/mL, Qiagen) for 20 min at 37°C while shaking (750 rpm) ...
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bioRxiv - Genomics 2021Quote: ... Reactions were incubated for 16 hours at 16 °C and were terminated by cleaning with miRNeay kit (Qiagen). Crosslink reversal was done by irradiating the RNA on ice 254 nm UVC for 5min using a CL-1000 crosslinker (UVP).
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-4 ml of cells were harvested at each timepoint and incubated with 2 volumes of RNAprotect (Qiagen) at room temperature for 5 minutes prior to centrifugation for 5 minutes at 4000xg 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease73 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease40 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Molecular Biology 2023Quote: ... and eluted in 16 μl EB (Qiagen). Libraries were assessed for quality and concentration on an Agilent Bioanalyzer instrument.
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bioRxiv - Genomics 2019Quote: ... eluted in 16 μL of buffer EB (Qiagen), then transferred to a fresh multi-well plate.
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bioRxiv - Cancer Biology 2021Quote: ... mRNA was enriched with Oligo-dT probes attached to Pure mRNA beads (Qiagen). RNA was fragmented ...
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bioRxiv - Evolutionary Biology 2022Quote: ... flavus spores derived from pure cultures using DNeasy UltraClean® Microbial Kit (Qiagen) with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was enriched with Oligo-dT probes attached to Pure mRNA beads (Qiagen). RNA was fragmented ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of fermentor culture was added to 4 mL RNAprotect Bacteria Reagent (Qiagen) and immediately vortexed for 10 sec ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 2 mL salts mixed with 4 mL of RNAprotect Bacteria Reagent (Qiagen), and then incubated for 5 min at room temperature for stabilization before centrifugation at 4000 rpm for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... FACS-sorted cells (≈ 98% tdTomato+ cells) were harvested in RLT and RNA extracted with RNeasy Micro kit (Cat. # Qiagen). Around 100 000 tdTomato+ cells were sorted from 10 electroporated-brains for each sample to sequence ...
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bioRxiv - Developmental Biology 2020Quote: ... 17 and 25 (hiPSC-derived myotube) using the miRNeasy Mini kit (217004, QIAgen) on the QIAcube instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... If the PCR product was pure then the sample was PCR purified (QIAGEN 28106) if the band was impure ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from pure lines organoids with the RNneasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... mRNA purification was performed with RNeasy Pure mRNA Bead Kit (Qiagen Cat. No. 180244). 28 ng mRNA was used to synthesize cDNA using 50 μM Oligo(dT)20 primers ...
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bioRxiv - Microbiology 2021Quote: ... Ribosomal RNA (rRNA) was depleted (Qiagen FastSelect5S/16S/23S) and cDNA libraries were generated (Qiagen Total Stranded RNA) ...
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bioRxiv - Microbiology 2022Quote: ... depletion was performed during library preparation using three probes from QIAGEN FastSelect rRNA 5S/16S/23S Kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Systems Biology 2023Quote: ... using the QIAseq FastSelect -5S/16S/23S kit (Qiagen). The transcriptomic reads were mapped to the combined sequence of the host genome and expression plasmid sequence by following the Modulome workflow (https://github.com/avsastry/modulome-workflow ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μl of bisDNA was added to 2 μl 10x PCR buffer (Qiagen, Cat# 203203), 2 μl of 10 μM primer mix (Methods Table 1) ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated for 2 h at 4 °C with Ni2+-NTA-agarose (Qiagen) (20 mg of proteins/ml of resin ...
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bioRxiv - Immunology 2021Quote: ... Skin samples were homogenized in a TissueLyser LT (Qiagen, 50 Hz, 2 times 4 minutes) using 5 mm stainless steel beads (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA from 2 to 4 organoids were isolated using the RNeasy mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... They produced milligram amounts and were purified to >95% using immobilized Ni-NTA chromatography (Qiagen) from a periplasmic extract of a 1 L culture ...
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bioRxiv - Microbiology 2022Quote: ... each sample was eluted in 40.5 µL elution buffer that was prepared by mixing 98 µL Buffer EB (Qiagen, CN 19086), 1 µL 10% Tween 20 (Teknova ...
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bioRxiv - Microbiology 2021Quote: ... were also extracted from pure cultures using the QIAamp BiOstic Bacteremia DNA kit (Qiagen, USA) according to the manufacturer’s instructions ...