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Citations for Qiagen :
101 - 150 of 6342 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 2 μl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was extracted from each sample using QIAgen DSP virus/pathogen Midi kits (QIAGEN) on a QIASymphonyXP laboratory automation instrument platform ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from 200 μL of specimen using the EZ1 DSP Virus kit (Qiagen) on the EZ1 Advanced XL instrument (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: Tissue homogenates for virus titration were generated using the Tissue Lyzer II (Qiagen, Gaithersburg, MD). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). qRT-PCR was then performed using AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... viral RNA was extracted from virus stocks using the Qiagen Viral RNA Mini Kit (Qiagen) and was used as a template for multi-segment RT PCR as described previously (31) ...
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bioRxiv - Genomics 2022Quote: ... Viral nucleic acids were then isolated using QIAamp MinElute Virus Spin Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions with some modifications described (Cosentino et al. ...
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bioRxiv - Microbiology 2023Quote: ... Samples for analysis of virus attachment were directly collected by incubating in RLT buffer (Qiagen) for 10 min at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and nucleic acid was extracted using EZ1 Advanced XL extraction with a Virus Card (QIAGEN). Six of the 20 FACS populations did not generate sufficient DNA and were not subsequently sequenced ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was performed on the QIAsymphony using the DSP Virus/Pathogen Mini Kit (Qiagen). Library preparation performed using Nextera XT (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: Viral genomic RNA was isolated from concentrated virus stocks with viral RNA isolation kit (Qiagen). RNA was treated with Turbo DNase and inactivation beads (Ambion) ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen, Toronto, ON, Canada). Then ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Phylloxera genomic DNA was extracted from 10 asexual female individuals using the DNeasy Blood & Tissue Kit according to the manufacturer’s protocol for total DNA purification from insects (Qiagen, Germantown, MD, USA). PCR was then used to amplify each of the four HGT genes from the genomic DNA with the primers listed in supplementary table S10 ...
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bioRxiv - Microbiology 2023Quote: DNA from insect samples and plants was isolated using the DNeasy Blood and Tissue or DNeasy plant kits (Qiagen, Valencia, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Cells were lysed for total protein in 96-well format using the Qproteome Mammalian Protein Prep Kit (Cat. 37901, Qiagen). Protein lysates were loaded onto and analyzed using the WesTM (ProteinSimple ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas3d and Cas10d proteins were purified from HEK293T cells expressing each His-tagged Cas protein using Ni-NTA agarose (Qiagen) and a gel filtration column (Superdex 200 increase 10/300 GL columns) ...
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bioRxiv - Biophysics 2020Quote: ... 45 min at 4 °C) and supernatant containing His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) purification (Qiagen). Protein was eluted in a final elution buffer of 20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... cell pellets were lysed in a 4% SDS buffer using a Qiashredder (Qiagen). Relative densitometry for western blots was determined using ImageJ software and normalized to the density of loading control β-ACTIN ...
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bioRxiv - Cell Biology 2019Quote: J774 cells were silenced using a pool of 4 different Flexitube siRNAs (Qiagen) for SYK with Lipofectamine RNAiMAX reagent as described previously 20 ...
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bioRxiv - Genomics 2021Quote: ... 4 μL of room temperature Stop solution (REPLI-g Single Cell Kit, Qiagen) was then added and the samples were vortexed and spun down ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with dsHsc70-4 with the Effectene Transfection reagent (301427, QIAGEN) and incubated for 72 h ...
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bioRxiv - Neuroscience 2023Quote: Cultured cells were lysed at 4°C in RLT buffer (Qiagen, Courtabœuf, France) and stored at -80°C ...
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bioRxiv - Genomics 2023Quote: Approximately 3-4 million cells were solubilized with 1 mL QIAzol (Qiagen, 79306) and 0.2 mL chloroform in 5PRIME Phase-Lock Gel heavy tubes (QuantaBio ...
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bioRxiv - Evolutionary Biology 2021Quote: We collected and froze F1 females after egg-laying for whole-body and ovary DNA extraction using the DNeasy® Blood &Tissue Kit following insect and Gram-negative bacteria protocols (Qiagen, Hilden, Germany). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... Viral DNA and RNA were extracted with the QIAamp MiniElute Virus Spin Kit (QIAGEN, Chatsworth, CA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Real-time one-step reverse transcription quantitative PCR was performed with the QuantiTect Virus Kit (Qiagen, Foster City ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted from supernatant culture media using the QIAamp 96 Virus QIAcube HT Kit (Qiagen). E-gene expression was determined using the SensiFAST Probe No-Rox One Step Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from apical washes using the QIAamp 96 Virus QIAcube HT Kit (QIAGEN). The SARS-CoV-2 genome was amplified using a highly multiplexed tiling PCR reaction based on the ARTIC protocol (https://www.protocols.io/view/ncov-2019-sequencing-protocol-bbmuik6w ...
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bioRxiv - Microbiology 2022Quote: ... The RNA of rescued virus strains was extracted with QIAmp DSP viral RNA mini kit (Qiagen), reverse transcribed and amplified with OneTaq one-step RT-PCR kit (NEB ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... RNAs were extracted from each amplified single virus using the QIAamp Viral RNA Mini Kit (Qiagen). Each gene segment was synthesized by gene specific primers (primers available upon request ...
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bioRxiv - Microbiology 2020Quote: ... using a QIAamp Mini Elute Virus spin kit for the viral RNA/DNA extraction (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... using a QIAamp Mini Elute Virus spin kit for the viral RNA/DNA extraction (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Qiasymphony automated platform was used to isolate total cellular RNA (DSP virus/pathogen mini kit (Qiagen). RNA was further processed in an in-house assay using primers of previously validated assays (44 ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from the virus sample with a QIAamp Viral RNA mini kit (QIAGEN). SARS-CoV-2 RdRP-2 gene primer probes sequences are as follows ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from each sample using the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany), and a semi-nested PCR assays was used for the detection of viral nucleic acids in each sample ...
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bioRxiv - Genomics 2021Quote: ... Remaining 140 µL of concentrated virus was processed according to QIAamp Viral RNA Mini kit (QIAGEN) protocol ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were extracted from specimens using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Hilden, Germany), QIAamp Viral RNA Mini Kit (Qiagen) ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: Total DNA was extracted from tissues using QIAamp MinElute Virus Spin Kit (57704, Qiagen, Venlo, Netherlands) or KingFisher Cell and Tissue DNA kit (97030196 ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s protocol for the QIAamp MinElute Virus Spin Kit (Cat. 57704, Qiagen, Hilden, Alemania). The resulting DNA for each sample was quantified with a Qubit fluorometer (Cat ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Synthetic Biology 2020Quote: ... and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN, Germany) equilibrated with purification buffer A ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Cys protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9-Cys wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... washed and RNA was extracted from 4×106 cells using the RNeasy kit (Qiagen) (performed in triplicate) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were treated with 4 μl of Qiagen protease (QIAGEN, Hilden, Germany, #1017782,) at 56°C for 10 min ...
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bioRxiv - Genomics 2020Quote: The DNA of VLPs was extracted following the manufacture’s protocol for the QIAampMinElute Virus Spin Kit (QIAGEN). The resulting DNAs were quantified (Qubit ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the sucrose-purified virus using the Qiagen DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: DNA from virus-infected plant tissues was extracted by DNeasy Plant Mini Kit (QIAGEN, Cat. No. 69104), and 500 ng of purified DNA was subjected to bisulfite treatment using EpiTect Plus DNA Bisulfite Kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: The viral RNA of the egg-passaged virus was extracted using QIAamp Viral RNA Mini Kit (Qiagen). The extracted RNA was then reverse transcribed to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) ...