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Citations for Qiagen :
51 - 100 of 6342 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... the insect tissue was homogenized and the genomic DNA was isolated using Qiagen Genomic-tip (Qiagen). Later ...
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bioRxiv - Microbiology 2020Quote: 200µL of lung clarified homogenate or infectious cell supernatant (virus stock) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from cell culture supernatants or hamster nasal washes using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... MB897-1) were used to generate infectious virus by transfecting 293T cells using the Effectene transfection reagent (QIAGEN, Valencia, CA). Supernatant containing virus was collected 2 days post transfection ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... round 1 and 3 virus infections of GeCKO-A549 cells using the midi gDNA extraction kit (Qiagen, Germantown, MD, USA). The sgRNA’s DNA copies were PCR amplified from the extracted gDNAs for next generation sequencing (Fig 1) ...
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bioRxiv - Microbiology 2024Quote: 200µL of lung of infectious cell supernatant (virus stock and HAE) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... total DNA was extracted from house flies according to the kits’ insect supplement guide (Qiagen, Germantown, MD). The manufacturer’s protocols were followed with the exception of using 100 μL of buffer for the elution step to extract a higher DNA concentration from our samples ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was isolated using EZ1 Virus Mini Kit v2.0 (QIAGEN). Quantification of viral RNA was performed using a real-time RT-PCR assay specific for HTNV nucleocapsid coding region in a LightCycler® 96 (Roche ...
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bioRxiv - Immunology 2020Quote: ... from mouse plasma using the QIAamp MinElute Virus Spin Kit (Qiagen) along with a DNaseI (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... Virus was inactivated by mixing 1:1 with Buffer ATL (Qiagen) prior to viral RNA extraction from NP swabs ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted using the QIAamp MinElute Virus Kit (Qiagen, #57704). Purified DNA was eluted in 50μl of Ultra-Pure Water (UltraPure™ DNase/RNase-Free Distilled Water ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using QIASymphony DSP Virus Pathogen Midi kit (Qiagen) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Cells were harvested for total protein using a Qproteome Mammalian Protein Prep Kit (Qiagen). Total protein was quantified using a PierceTM BCA Protein Assay Kit (Cat ...
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bioRxiv - Cell Biology 2023Quote: Cells were collected for total protein using Qproteome Mammalian Protein Prep Kit (Cat. 37901, Qiagen). Lysates were quantified for protein expression utilizing the WES system (ProteinSimple ...
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bioRxiv - Zoology 2021Quote: Nucleic acids were extracted using a QIAamp MinElute Virus Spin Kit (QIAGEN) and used to construct the sequencing libraries ...
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bioRxiv - Plant Biology 2021Quote: ... Virion DNA was isolated using QIAamp MinElute Virus Spin Kit (Qiagen, Maryland).
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bioRxiv - Immunology 2022Quote: ... To sequence the envelope from serum virus we isolated viral RNA (Qiagen), synthesized cDNA ...
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bioRxiv - Immunology 2020Quote: Plasma RNA was extracted using the QIAamp MinElute Virus Spin Kit (Qiagen) along with a DNaseI (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... qPCR of SIV gag RNA was by QuantiTect Virus kit (Qiagen 211011) or ddPCR using One-Step RT ddPCR Adv kit (Bio-Rad 1864022) ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR of SIV gag RNA was by QuantiTect Virus kit (Qiagen 211011) or ddPCR using One-Step RT ddPCR Advanced Kit for Probes (Bio-Rad 1864022) ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen). Then ...
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bioRxiv - Molecular Biology 2019Quote: ... and virus RNA was purified by using the QIAamp viral RNA kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was extracted from the virus preparation using the RNeasy kit (Qiagen). The first cDNA strand corresponding to the entire 10 kb-long genomic RNA was produced using Superscript III Reverse transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from virus stock using QIAamp Viral RNA Mini Kit (Qiagen) and treated with TURBO DNase (Ambion) ...
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bioRxiv - Immunology 2019Quote: Viral RNA was isolated from virus particles with RNeasy-Kit (Qiagen, Valencia, CA). Access RT-PCR kit (Promega ...
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bioRxiv - Neuroscience 2020Quote: SIV RNA was measured by qRT-PCR using the QuantiTech Virus Kid (Qiagen) and the following primers and probes in the SIV gag region (Fwd ...
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bioRxiv - Microbiology 2020Quote: ... RNA of single virus clone was extracted by Viral RNA Mini Kit (QIAGEN) and reversely transcribed using PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TaKaRa ...
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Genetic heterogeneity in the Salmonella Typhi Vi capsule locus: A population genomic study from FijibioRxiv - Microbiology 2023Quote: ... DNA was extracted using a QIAsymphony DSP virus pathogen kit (Qiagen, Maryland, USA). Genome sequencing was performed using Illumina Nextera XT (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: Total DNA was extracted from insect tissue using a DNeasy®Blood & Tissue Kit according to manufacturer instructions (QIAGEN, Australia). Crude extractions were conducted using a polypropylene pellet pestle (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... and the homogenates were used for DNA extraction following the insect tissues protocol of the DNeasy Blood and Tissue (Qiagen) kit ...
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bioRxiv - Evolutionary Biology 2024Quote: Adult insects stored at -80°C were crushed (between 80 mg to 4650 mg of insects depending on the species) with a “Tissue-Lyser” apparatus (Qiagen) in a buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... 4 μL of Buffer D2 (REPLI-g Single Cell Kit, Qiagen) and 1 μL of 500 μM exonuclease-resistant random primer were then added to the lysed cells to denature the DNA prior to vortexing ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was isolated using the QIAamp MinElute Virus Spin Kit (Qiagen, Germantown, MD), omitting carrier RNA ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from 200µl serum using the EZ1 Virus Mini Kit v2.0 (Qiagen), and viral load determined by Artus HBV PCR kit (Qiagen).
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bioRxiv - Evolutionary Biology 2020Quote: RNA extracts in which the virus was detected were DNAse I treated (Qiagen, Germany) for 15 min at room temperature prior to sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was isolated using the QIAamp MinElute Virus Spin Kit (Qiagen, Germantown, MD), omitting carrier RNA ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was subsequently isolated from mouse plasma using the MinElute Virus Kit (Qiagen) on the QiaCube ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from virus supernatants using the QIAmp Viral RNA kit (Qiagen, UK) as described by the manufacturer (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA from the NPA was extracted using EZ1 Virus Mini Kit v2 (Qiagen [955134]). The RNA concentration was measured by the Qubit RNA BA assay (Qiagen [32852]) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from 100 μl of the virus stock using RNeasy kit (Qiagen) and analyzed by RT-qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... rAAV genomes were extracted and purified using a QIAamp MinElute Virus Spin kit (Qiagen) and titered by qPCR with serial dilutions of a plasmid standard ...
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bioRxiv - Genomics 2022Quote: ... DNA was then extracted immediately with QIAamp UltraSens Virus Kit (QIAGEN, Cat. No. 53706). The manufacturer’s instructions were followed with the first six steps replaced by combining 140 μL of sample with 5.6 μL carrier RNA and vortexing briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins from HGCC cells and GBM tissue were extracted with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Cancer Biology 2022Quote: SUZ12 isogenic M3 MPNST cells were treated with or without DNMT inhibitors (50 nM daily Decitabine or 4 μM single dose of GSK862) for 4 days followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Bisulfite sequencing was conducted by the IGO core facility at MSKCC ...
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bioRxiv - Molecular Biology 2022Quote: ... HL-1 cells were transduced with a pool of 4 gapmers (Qiagen) at 40nM (10Nm each ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen), the eluted fractions containing S protein were pooled ...
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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bioRxiv - Cancer Biology 2021Quote: ... DNA was isolated from 4×107 KPC cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen). NGS libraries were prepared using the following primers:
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bioRxiv - Molecular Biology 2020Quote: ... RNA from these samples was obtained with the QIAamp Mini Elute Virus Spin Kit (Qiagen) following the manufacturer’s protocol ...