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Citations for Qiagen :
651 - 700 of 1338 citations for Chikungunya Virus E2 Envelope Protein HEK293 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with Ni-NTA agarose resin (Qiagen, 30210) for four hours ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen). Proteins were eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... All proteins were purified under native conditions by using Ni-NTA sepharose (QIAGEN) according to the manual.
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bioRxiv - Immunology 2021Quote: ... The proteins from filtered culture supernatants were purified by Ni-NTA column (QIAGEN) and size exclusion chromatography (HiLoad Superdex 75 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified by metal affinity chromatography on a Ni2+-NTA column (Qiagen). Cell free extract was loaded onto column followed by two wash steps with wash buffer (Tris 50 mM ...
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bioRxiv - Biochemistry 2021Quote: ... Briefly proteins were purified by glutathione-Sepharose (Cytiva) or Ni-NTA agarose (Qiagen) affinity chromatography ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged fusion proteins were purified with a Ni-NTA column (Qiagen), and the elution fraction was incubated with TEV protease for 15-18 h at 4°C to cleave the N-terminal His-tag ...
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bioRxiv - Microbiology 2021Quote: ... and the protein was purified using a Ni–NTA column (Qiagen, Hilden, Germany) as reported previously 49 ...
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bioRxiv - Microbiology 2020Quote: ... The RBD protein was purified by affinity chromatography using Ni-NTA resin (QIAGEN), followed by size exclusion chromatography on a HiLoad Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble recombinant protein was purified using theNickel-Nitrilotriacetic acid (Ni-NTA+; Qiagen) resin ...
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bioRxiv - Cell Biology 2021Quote: ... The protein interaction network analysis was performed using QIAGEN’S Ingenuity Pathways Analysis (QIAGEN’S Ingenuity pathway analysis ...
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bioRxiv - Bioengineering 2022Quote: ... Purification of all proteins was performed using a Ni-NTA agarose column (Qiagen). After washing with buffer A containing 30 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Plant Biology 2022Quote: ... Target protein was recovered and purified with a His-Trap affinity column (QIAGEN) according to manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2023Quote: The eluted protein was mixed with 1 ml Strep-Tactin Superflow agarose (Qiagen) and incubated at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was isolated from the lysate using Ni-NTA Agarose resin (Qiagen) and was purified to homogeneity and >95% purity by size exclusion chromatography using a HiLoad Superdex 75 pg 16/600 column (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified through metal-affinity chromatography using Ni2+–NTA agarose beads (Qiagen). Purified protein aliquots were stored at -30°C in 50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2023Quote: ... all proteins were purified using Ni-NTA (nickel-nitrilotriacetic acid) resin from QIAGEN. After the incubation of Expi293 media supernatant with the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Preparation of his-tagged recombinant proteins was performed according to manufacturer instructions (Qiagen). Preparation of GST-tagged recombinant proteins was performed according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under non-denaturing conditions using Ni-NTA resin (Qiagen) at 4 °C according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Physiology 2023Quote: ... Tissues were then lysed in protein isolation buffer using a tissue-lyser (Qiagen). Protein concentration was measured by Bradford assay ...
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bioRxiv - Biophysics 2023Quote: ... His6-MBP-HNRNPH2 proteins were first purified using Ni-NTA Agarose (#30230, Qiagen) and eluted with buffer containing 250 mM imidazole pH 7.8 (#79227 ...
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bioRxiv - Cancer Biology 2022Quote: ... EV and protein fractions using miRNeasy serum/plasma kit (Qiagen 1071073 Lot#160020206) after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035) ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Nickel sepharose according to the manufacturer’s instructions (QIAGEN). The purified recombinant protein was dialyzed overnight against dialysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by immobilized metal affinity chromatography using Ni–NTA agarose (Qiagen) pre-equilibrated with lysis buffer in individual Econo-Pac gravity-flow columns (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: SAINT Express protein hits were analyzed using Ingenuity Pathway Analysis (IPA) software (Qiagen). Significant hits were analyzed and enrichment z-score was calculated for different pathways ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant TDP-43 proteins were purified over Ni-NTA agarose beads (Qiagen) and eluted using 50 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... All nucleotide and protein sequences were analyzed using CLC Main Workbench 8.1.2 (Qiagen).
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Neuroscience 2023Quote: Protein pathway analysis was conducted on DEP using Ingenuity Pathway Analysis (IPA, Qiagen) and Gene Ontology (GO ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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bioRxiv - Molecular Biology 2020Quote: ... miRCURY LNA Power Inhibitor against mouse miR-375 (mmu-miR-375-3p) (Qiagen, Hilden ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from mouse liver samples using RNeasy Mini Kit (Qiagen), DNase treated and 1 μg total RNA was processed for mRNA sequencing ...
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bioRxiv - Neuroscience 2020Quote: Pre-weighed cortex pieces from mouse brains were homogenized in QIAzol (Qiagen 79306), at 1ml volume per 100mg of tissue ...
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bioRxiv - Physiology 2020Quote: Total RNA was isolated from mouse tissues using QIAzol Lysis Reagent Protocol (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA of mouse islets was extracted using RNeasy Plus Mini Kit (Qiagen). Other tissues including mouse liver ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from mouse right adrenals using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from mouse right adrenals using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA from mouse liver was isolated using RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: We analyzed human and mouse Tug1 cDNA sequences with CLC Genomics Workbench (Qiagen) for open reading frames (ORFs) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... we prepared total RNA from dissected wildtype mouse brain regions using Qiazol (Qiagen) and prepared cDNA using the SuperScript III first-strand synthesis kit (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: Total RNA was extracted from mouse pituitaries using an RNeasy mini kit (Qiagen) and 250 ng converted to cDNA using Revertra Ace (TOYOBO ...
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bioRxiv - Genetics 2020Quote: Mouse tissues were disrupted and homogenized using QIAzol lysis reagent (Qiagen, Hilden, Germany) and the Tissue Lyser instrument (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... or RNeasy Mini Kit (synaptosome and cytosolic fraction from homogenized mouse forebrain; Qiagen). For RT-PCR ...
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bioRxiv - Immunology 2020Quote: RNA from mouse plasma was isolated using Qiamp RNA-mini isolation kit (Qiagen) and RNA from tissues isolated using the RNeasy mini isolation kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was isolated from mouse B cells using the DNeasy kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from mouse neural retina using RNeasy mini kit (QIAGEN). Total RNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time ...
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bioRxiv - Genomics 2023Quote: We extracted RNA from fresh mouse using an AllPrep DNA/RNA kit (Qiagen). We placed 5-10 mg of tissue into 350 μL RLT Plus Buffer in a 2 mL tube containing a 5mm stainless steel bead and homogenized with a TissueLyzer for two 2 min rounds at 30 Hz ...