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Citations for Qiagen :
601 - 650 of 1338 citations for Chikungunya Virus E2 Envelope Protein HEK293 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Next RNA and protein were digested and DNA was recovered by Qiagen PCR purification kit ...
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bioRxiv - Cell Biology 2023Quote: ... His10 proteins were purified by using Ni-NTA agarose (QIAGEN, Catalog # 30230), followed by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then purified with Ni-NTA agarose beads (QIAGEN; 30210) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). Eluted protein was concentrated and passed over a Superdex 200 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... After the separation of nanodiscs and soluble proteins on Ni-NTA (Qiagen), the same proportions of unbound material and imidazole-eluted material were analyzed (Fig.S5) ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tagged proteins were purified with HisBind NiNTA-agarose (Qiagen) according to the recommendations of the supplier ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from mouse tissues using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... ribodepletion was performed using the Fast Select mouse RNA removal kit (Qiagen), the RNA-seq libraries were prepared according to manufacturer’s instructions using the Illumina TruSeq Stranded mRNA Kit and 50 bp single reads sequencing was performed with the Illumina HiSeq 4000 Sequencer ...
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bioRxiv - Systems Biology 2019Quote: ... Total RNA was isolated from mouse liver with miRNeasy Mini Kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The mouse RNA sample was isolated using Qiagen RNAeasy Plus Kit (Qiagen). The RNA concentration was determined using Qubit 4 Fluorometer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system under these cycling conditions ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was isolated from mouse tissues using RNeasy Mini Kit (Qiagen) or RNA STAT-60 (Tel-test ...
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bioRxiv - Genetics 2021Quote: ... Hrh1 amplicons from each mouse strain were gel purified (Qiagen Cat# 28115) and DNA sequencing reactions were performed with the BigDye terminator cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA against 3’UTR sequence of mouse Alr were purchased from Qiagen. siRNAs were transfected into HEK293 or MEF using Dharmafect 1 Transfection Reagent (Dharmacon) ...
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bioRxiv - Molecular Biology 2022Quote: ... or isolated primary mouse macrophages using RNeasy Mini Kit (Qiagen, Cat# 74106) and reverse-transcribed using High-Capacity cDNA Reverse Transcription Kits (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected into mouse calvarial osteoblasts using the Effectene transfection reagent (Qiagen). After 48 hours ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tag ...
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bioRxiv - Immunology 2020Quote: ... bulk DNA was isolated from mouse pellets (DNeasy Powersoil Kit, Qiagen, Inc) and attempts were made to amplify a 16S rRNA locus using broad-range (universal ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse penta-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Immunology 2019Quote: ... The following mouse primer sets were used from Qiagen (Valencia, CA, USA): TBP (PPM03560F ...
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bioRxiv - Cell Biology 2021Quote: siRNA for mouse VHL (Flexitube Gene Solution GS22346) was obtained from Qiagen. AllStars negative control siRNA ...
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bioRxiv - Neuroscience 2020Quote: ... mouse RNA from V1 was extracted using the miRNeasy Mini Kit (Qiagen) based on manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... human sputum cells or mouse lung tissue using an RNeasy kit (Qiagen). 2μg was utilised for cDNA synthesis using the Omniscript RT kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: A RT2 profiler array for mouse inflammatory cytokines and receptors (Qiagen, USA) was used according to manufacturer recommendations to measure the expression of 84 inflammatory genes (Table S1) ...
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bioRxiv - Genetics 2022Quote: ... mouse brain and brain region tissues using the miRNeasy kit (QIAGEN, # 217004) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Mouse antibody genes were amplified using HotstarTaq DNA polymerase (Qiagen Cat # 203209) with the primer sets specific for the IghIOMAiGL and IgkIOMAiGL transgenes ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from mouse cells using the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... a conjugate of mouse monoclonal penta-His antibody and horseradish peroxidase (Qiagen) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse tissue RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106) following the manufacture’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... and mouse anti-Phospho-Serine Q5 (catalogue number: 37430) was from Qiagen. Horseradish peroxidase-conjugated secondary antibodies against rabbit (catalogue number ...
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bioRxiv - Genetics 2023Quote: Mouse tissues were either homogenized using QIAzol lysis reagent (Qiagen, Hilden, Germany) in a Tissue Lyser instrument (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... A mouse Yy1-specific siRNA or the AllStars Negative Control siRNA (Qiagen) was co-transfected with an L1 promoter reporter plasmid using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified using a Ni-NTA Superflow column (Qiagen, Netherlands, 30761) on a Äkta Express fast protein liquid chromatography system (FPLC ...
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bioRxiv - Microbiology 2019Quote: ... the fusion protein was purified using His-Bind columns (Qiagen, Venlo, The Netherlands) and analyzed by SDS-PAGE using gels containing 12% polyacrylamide ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transiently transfected with mammalian protein expressing plasmids using Effectene (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged proteins were purified on Ni-NTA agarose (Qiagen; catalog no. 30761) and GST-tagged TopBP1 protein was purified on Glutathione Sepharose™ 4B (GE healthcare ...
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bioRxiv - Genomics 2019Quote: ... RNA attached to purified protein complexes was isolated with the miRNeasy kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: DNA was extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Expressed proteins were subsequently purified by Ni-chelate chromatography (Qiagen, 5 ml column) and SEC (S75 ...
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bioRxiv - Biochemistry 2020Quote: ... and the soluble protein fractions were purified by Ni-NTA resin (Qiagen, # 30230). The purified fraction was concentrated ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified with nickel chelate affinity resins (Ni-NTA, Qiagen). The protein samples were collected and purified by a Superdex 200 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen), amylose (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Microbiology 2021Quote: ... Whole DNA and proteins were extracted by the ALLprep Kit (Qiagen, Hilden, Germany), following manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen) following the instructions of the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Plant Biology 2020Quote: ... and the GST fusion proteins were purified by Glutathione HiCap Matrix columns (Qiagen) and eluted using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was analyzed by immunoblotting using monoclonal anti-RGSH4 antibodies (Qiagen) to detect ABCG5 and polyclonal anti-hABCG8 antibodies (Novus Biologicals ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The recombinant proteins were purified under denaturing conditions on Ni-NTA agarose (Qiagen). The purified proteins were used for rat immunization in an in-house animal facility at the Charles University.