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Citations for Qiagen :
51 - 100 of 1473 citations for Calcium calmodulin Dependent 3' 5' Cyclic Nucleotide Phosphodiesterase 1C PDE1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward: 5’-GGCACCGAGAAAGACAAAGT-3’; reverse: 5’-CTCTGGAAATGGGCTTCAAA-3’) and PCR products were purified using QIAquick PCR purification kit (QIAGEN, Cat #28104) before Sanger sequencing ...
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bioRxiv - Genomics 2019Quote: In order to screen for the presence of the Alu element in exon 4 of RP1 distinct pair of primers were designed (forward: 5′-AGGCTTGTTTCCTAGGAGAGGT-3′, reverse: 5′-TTCTGCTTCTTTTTCACTTAGGC-3′) using the CLCbio Genomics Workbench (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: ... region was amplified using fungal-specific primers (60): ITS1F (5’-CTTGGTCATTTAGAGGAAGTAA-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) and the HotStarTaq Plus Master Kit (Qiagen, Valencia, CA). Amplicons from different samples were pooled to equimolar concentrations and purified of short fragments using Agencourt Ampure beads (Agencourt Bioscience Corporation ...
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bioRxiv - Microbiology 2022Quote: ... of the 16S rRNA gene that amplified via PCR using primers 338F (Seq: 5’-ACTYCTACGGRAGGCWGC-3’) and 1061R (Seq: 5’-CRRCACGAGCTGACGAC-3’) with the HotStar HiFidelity Polymerase Kit (catalog number 202602; QIAGEN, Hilden, Germany). Supplementary Figure 1 shows the 16S rRNA gene map and the primers used ...
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bioRxiv - Microbiology 2024Quote: ... and homogenized in groups of 3-5 flies by bead-beating with 3 x 5 mm stainless steel beads (Qiagen, Hilden, Germany) in 1 ml 1X PBS ...
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bioRxiv - Microbiology 2019Quote: ... and further purified with the QIAquick Nucleotide Removal Kit (Qiagen). Both steps were performed following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... NHS was removed with QIAquick Nucleotide removal kit (Qiagen, 28304) and the conjugated JF-669 hairpins were reconstituted with nuclease free water to a final concentration of 60 ng/ml (3 mM).
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bioRxiv - Molecular Biology 2022Quote: ... followed by purification using a QIAquick Nucleotide Removal Kit (QIAGEN) and eluted with H2O ...
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bioRxiv - Genomics 2024Quote: ... Unincorporated nucleotides were removed using QIAquick PCR Purification Kit (Qiagen). Probe size (50-500 bps ...
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bioRxiv - Cell Biology 2019Quote: ... Kif5b#4 (target sequence 5′-CACGAGCTCACGGTTATGCAA-3′; Qiagen SI00176057), and non-target (NT ...
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bioRxiv - Cell Biology 2021Quote: ... 5’-ATGATCGATCATCTATAGCAA-3’ and 25nM S1PR1 #2 (Qiagen, #SI00376208) 5’-TAGCATTGTCAAGCTCCTAAA-3’ siRNAs or AllStars Negative Control siRNA (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... DNA was cleaned up with the QIAquick Nucleotide Removal Kit (Qiagen), and the concentration measured by UV absorption at 260 nm in a NanoDrop 2000c Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The probes were then purified using Qiaquick Nucleotide Removal Kit (Qiagen), and eluted in 150 μl of Elution Buffer ...
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bioRxiv - Biophysics 2023Quote: ... Labeled oligos were purified with QIAquick nucleotide removal kit (Qiagen; #28304) and used as reverse transcription primers.
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bioRxiv - Cancer Biology 2021Quote: ... a 19-mer SPRY2 target sequence (5′-AACACCAATGAGTACACAGAG-3) (QIAGEN) was used and for the control a 19-mer NSi control sequence (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... For Astrin siRNA oligo #367 (5′-TCCCGACAAC TCACAGAGAAA -3′)(Qiagen) was used as described (Thein et al. ...
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bioRxiv - Genetics 2021Quote: ... Unincorporated [γ-32P]-ATP was removed using a nucleotide removal column (Qiagen). The binding reaction was done as described in the EMSA section ...
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bioRxiv - Immunology 2022Quote: ... The PCR products were purified with the Nucleotide Removal Kit (Qiagen, 28304). Amplified double-stranded DNAs were digested with FastDigest BpiI (BbsI ...
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bioRxiv - Biophysics 2019Quote: ... The modified oligonucleotides were purified using a Qiaquick nucleotide removal kit (Qiagen) and hybridized respectively with 26XhoI-B or 88.XbaI C ApaI (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... the final nucleosomal DNAs were recovered by QIAquick Nucleotide Removal kit (QIAGEN) and used for library preparation ...
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bioRxiv - Genomics 2024Quote: ... Reactions were purified with the QIAQuick Nucleotide Removal Kit (Qiagen, Hilden, Germany), pooling eight reactions per column and eluting each in 30 µL ...
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bioRxiv - Microbiology 2020Quote: ... CjNC110-LNA DIG-labeled probe (/5’DigN/ GCACATCAGTTTCAT/3’Dig_N/) (QIAGEN) was added to 15 mL DIG EasyHyb™ Buffer (Roche ...
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bioRxiv - Systems Biology 2022Quote: ... 5 μl of proteinase K (approximately 3 U, Qiagen, cat. # 19131) were added and incubated for 1 h at 56°C ...
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bioRxiv - Cell Biology 2021Quote: ... 5’-TAGCATTGTCAAGCTCCTAAA-3’ siRNAs or AllStars Negative Control siRNA (Qiagen, #1027281). After 72 h ...
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bioRxiv - Cell Biology 2023Quote: ... using 30 nM Hs_LUZP1 siRNA (target sequence, 5′-CAGCGGGTGCTGAGAATTGAA-3′; QIAGEN) or 30 nM AllStars negative-control siRNA (QIAGEN) ...
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bioRxiv - Microbiology 2023Quote: ... and homogenised with 3-5 mm steel beads in a TissueLyser II (Qiagen, 24 Hz, 2 × 3 min). 350 μL lysed blood or 200 μL lysed head kidney were transferred to a Magna Pure 96 Processing Cartridge (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using a using a nucleotide removal kit (Qiagen), phosphorylated using T4 polynucleotide kinase (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Reaction was then cleaned up using the Nucleotide Removal Kit (Qiagen, Cat #28304) as follows ...
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bioRxiv - Cell Biology 2020Quote: ... ChIPed DNA was purified by using QIAquick® Nucleotide Removal Kit (28306, Qiagen) according to the manufactor’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... All nucleotide and protein sequences were analyzed using CLC Main Workbench 8.1.2 (Qiagen).
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bioRxiv - Cell Biology 2021Quote: The siRNA sequence (5’-GAGUAGAACUAGAAUGUGA-3’) targeting Hec1 was synthesized by Qiagen. The ON-TARGETplus SMARTpool siRNA sequences (5’-GAACGAGUAACCACAAUUA-3’) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using siRNAs targeting MAD2 sequence 5′-AGAUGGAUAUAUGCCACGCTT-3′ (Qiagen), pools of siRNAs targeting the TP53 sequence 5’-GAAAUUUGCGUGUGGAGUA-3’ ...
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bioRxiv - Genomics 2021Quote: ... The scramble-miR miRCURY LNA Detection probe (5’-GTGTAACACGTCTATACGCCCA-3’, YD00699004, QIAGEN) was used as a negative control ...
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bioRxiv - Genetics 2020Quote: ... The 3’ and 5’ – DIG labeled miRCURY LNA miRNA Detection probes (Qiagen, negative control Scramble-miR cat ...
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bioRxiv - Microbiology 2022Quote: ... Single-nucleotide polymorphism (SNP) detection was performed by applying CLC Genomics workbench (v10.1.1, Qiagen). Paired reads were mapped to the annotated reference genome of S ...
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bioRxiv - Biochemistry 2023Quote: ... The digested genomic DNA was then purified by QIAquick Nucleotide Removal Kit (QIAGEN, #28306) and used as an input for the pulldown-seq.
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bioRxiv - Plant Biology 2020Quote: Total RNA isolated from 3-week-old thalli (QIAGEN RNAeasy Plant Kit 5) from each genotype was assessed for quality with a Nanodrop spectrophotometer and a Bioanalyzer 2100 microfluidics system (Agilent 6) ...
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bioRxiv - Pathology 2021Quote: PDGFRα+ and CD56+ cells (passages 3 to 5) were lysed in Qiazol (Qiagen) followed by chloroform/isopropanol total RNA extraction ...
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bioRxiv - Microbiology 2019Quote: ... Real-time PCR assays were carried out in triplicate to amplify the Wolbachia wsp gene [43] and host reference gene GAPDH (378 F_ 5’-CCGGTGGAGGCAGGAATGATGT-3’, 445 R_5’-CCACCCAAAAGACCGTTGACG-3’) on a Rotor-gene Q Instrument (Qiagen, NSW, Australia). Reactions were run in a total volume of 10 μl having 5 μl Rotor-Gene SYBR® Green PCR Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was then extracted from the cytosolic fraction using the QIAquick Nucleotide Removal kit (QIAGEN) and from the whole cell fraction using the QIAamp DNA Mini Kit (QIAGEN) ...
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bioRxiv - Immunology 2023Quote: ... plasmid preparation and DNA gel extraction were performed using nucleotide purification kits purchased from Qiagen or BioBasics ...
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bioRxiv - Neuroscience 2022Quote: ... and 18S rRNA (5’-GAG CGA AAG CAT TTG CCA AG-3’ and 5’-GGC ATC GTT TAT GGT CGG AA-3’) on a Roter-Gene Q (Qiagen, Hilden, Germany) with 2x AmpiGene® qPCR Green Mix Lo-ROX (Enzo Biochem ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were reverse transfected with 5 pmol of each siRNA (QIAGEN) using lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Circular DNA was purified using the QIAquick nucleotide removal kit according to the manufacturer’s protocol (Qiagen).
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bioRxiv - Cell Biology 2019Quote: ... Cy3-labeled miR-430 was separated from unconjugated dyes using a QIAquick Nucleotide Removal Kit (QIAGEN).
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bioRxiv - Molecular Biology 2019Quote: ... small fraction RNA (≤200 nucleotides) extraction was performed separately using RNeasy Mini Elute Cleanup Kit (QIAGEN). Both total and small RNA extraction was performed according to the user manual ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of RGS(His)6-tagged 14-3-3 was performed by applying the anti-RGS(His)6 antibody (Qiagen) in combination with a secondary anti-mouse HRP antibody.
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
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bioRxiv - Genomics 2020Quote: ... and also performed direct mapping against reference genome Wuhan/Hu-1/2019 (NCBI Nucleotide – NC_045512, GenBank – MN908947) using the CLC Genomics Suite v5.1.0 (QIAGEN). We then used SAMtools v1.3 to sort the aligned bam files and generate alignment statistics (WysokerA ...
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bioRxiv - Microbiology 2022Quote: ... The products were separated on an 1.5% agarose gel and purified using the Nucleotide removal kit (Qiagen). The ina gene sequence was obtained from and an E ...