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Citations for Qiagen :
651 - 700 of 964 citations for CTLA 4 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from ten 4-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Pathology 2024Quote: ... by running one homogenization cycle (50 Hz oscillation frequency (50 cycles/s) for 4 minutes) in a Tissue lyser LT (Qiagen). Tubes were then centrifuged at 5000 rpm for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
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bioRxiv - Immunology 2024Quote: ... Cytosolic fractions were prepared by centrifugation at 10,000 × g for 30 min at 4°C and DNA was isolated from them using the DNeasy Blood & Tissue kit (Qiagen). The copy number of mitochondrial DNA encoding 16S RNA (RNR2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was added to a 500 μl equilibrated Glutathione Sepharose 4 Fast Flow affinity medium (GE) or Ni-NTA agarose (Qiagen). The lysate was incubated with an affinity matrix for 1 hour at 4 ⁰C ...
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bioRxiv - Genomics 2024Quote: ... Digested product was then run on a 4% agarose gel with 1x SYBR Safe for 45 minutes at 100 V and gel extracted (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... Lysates were centrifuged for 30 min at 30000 x g in 4 °C and the resulting supernatants were gently mixed with Ni-NTA Agarose beads (Qiagen) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Lysed cells were sedimented by centrifugation at 20,000 RCF at 4 °C for 30 min and the lysate was subsequently loaded onto pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The cell pellet was removed after centrifugation (16000 rpm, 4 °C, 1 h) and supernatant was loaded into a Ni-NTA agarose column (Qiagen). Protein was eluted in an elution buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Genomics 2024Quote: ... Fosmid clones from the WIBR-1 library were purchased from the BACPAC Resources Center (for coordinates and names, see Supplementary Table 4) and isolated using Large-Construct Kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: ... Chemokine expression was quantified using RT2 Profiler PCR array for mouse chemokines/cytokines (Qiagen PAMM-150ZA, 330231) with data analysis performed using the Qiagen online tool PCR Array data analysis Web portal (https://www.qiagen.com/gb/resources/resourcedetail?id=20762fd2-8d75-4dbe-9f90-0b1bf8a7746b&lang=en) ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was extracted from STHdh cells and mouse striatal tissues by using the RNeasy extraction kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... as annotated in miRBase Release 16 (www.mirbase.org) was then performed using miScript Mouse miRNome PCR array (V16.0, 96-well/Rotor-Disc 100; Qiagen) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from mouse lung tissue according to the RNeasy Plus with gDNA removal protocol (QIAGEN) and quantified using an HS RNA kit and Fragment Analyzer (Advanced Analytical) ...
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bioRxiv - Molecular Biology 2022Quote: DNA of the mouse fecal microbiota was isolated by using QIAamp Fast DNA Stool Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from 3D cultures of mouse primary keratinocytes using RNeasy Micro Kit (QIAGEN, UK), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: Total DNA was extracted from mouse fecal samples using QIAamp DNeasy Blood and Tissue Kit (Qiagen, UK) according to the manufacturer’s protocol and as previously described57 ...
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bioRxiv - Pathology 2022Quote: DNA was extracted from mouse tails using the DNeasy® Blood & Tissue Kit (Qiagen®, Hilden, Germany) and DNA concentration was measured with NanoDrop ND-1000 (Thermo-Fisher Scientific Inc.) ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from AML12 cells and mouse livers using RNeasy Mini Kit (Qiagen, Venlo, Nederland) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from rat or mouse hippocampal cultures using the RNeasy Mini Kit (Qiagen, #74104). DNA contamination was removed by digestion with DNase I (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from the mouse lenses using the RNeasy Plus Micro Kit (QIAGEN, Venlo, Netherlands) per the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: 500ng mouse or 300ng sheep RNA was reverse transcribed using QuantiTect Reverse transcription kit (Qiagen, Manchester, UK) and gDNA wipe-out (to remove genomic DNA) ...
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bioRxiv - Physiology 2021Quote: ... Mouse C2C12 myotubes: Total RNA was extracted using TRIzol and purified using RNeasy Mini spin columns (QIAGEN). Reverse transcription was performed using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
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bioRxiv - Neuroscience 2022Quote: RNA purification from both IPSC and primary mouse cortical cultures were done using RNeasy 96 Kit (Qiagen) according to the manufacturer protocol ...
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bioRxiv - Pathology 2022Quote: ... and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen). The isolated RNA was transcribed to complementary DNA via Superscript IV VILO Master Mix (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from transfected 293T cells and frozen mouse testes using RNeasy Mini kit (QIAGEN). Extracted RNA was used for cDNA synthesis using iScript cDNA Synthesis kit (BIO-RAD ...
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bioRxiv - Biochemistry 2023Quote: Genomic DNA (i.e., gDNA) was isolated from mouse liver tissue using the DNeasy Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from one quarter of a mouse kidney using RNeasy Plus Mini Kit (Qiagen, 74134). For quantitative real-time PCR ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from mouse cecal contents and in vitro cultures using the RNeasy PowerMicrobiome Kit (Qiagen). Ribosomal RNA depletion was performed using the RiboMinus™ Transcriptome Isolation Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: Mouse primary hippocampal neurons grown on coverslips were transiently transfected at DIV11 with Alexa 488-labeled siRNA either specifically targeting mouse Kcc2b (Mm_Slc12a5_1 FlexiTube siRNA, GeneGlobe ID: SI01419243, GenBank accession no: NM_020333; Qiagen) or Ank3 mRNA (Mm_Ank3_3 FlexiTube siRNA ...
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bioRxiv - Microbiology 2023Quote: Mouse cDNA was amplified with gene-specific primers (see below) and a HotStarTaq Master Mix Kit (Qiagen). Amplicons were cloned and inserted into a pGEM-T vector (Promega ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from de-identified tubes of mouse tissues using the RNeasy Kit (QIAGEN; Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and the pellet was lysed using RLT buffer (mouse cells) or QIAZol (human cells) (Qiagen, Cat. 79306). RNA was extracted using the Qiagen RNeasy micro kit.
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bioRxiv - Cell Biology 2023Quote: ... and reads were aligned to the mouse reference genome Refseq GRCm39.105 from the Biomedical Genomics Analysis Plugin 20.0.1 (Qiagen). Normalization of RNA-seq data was performed using trimmed mean of M-values ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA extraction was done on mouse kidney lysate using the RNeasy Plus Universal Midi kit (Qiagen). RNase digestion was then performed on the RNA extract using the following RNases ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from mouse tissue samples using an AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA). Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen ...
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bioRxiv - Physiology 2024Quote: Total RNA from mouse hearts (normal, ischemic, or non-ischemic) was isolated using RNeasy Mini kits (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Eukaryotic RNA was isolated from mouse tissue samples (stomach and colon) stored in RNAprotect Tissue Reagent (Qiagen) at ™70 °C before extraction using a GeneJET RNA Purification Kit (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... Luciferase reporter constructs were either mock-treated or methylated in vitro with SssI CpG methyltransferase for 4 h at 37 °C and purified with the QIAquick Purification Kit (QIAGEN 28704). Reporter plasmid (500 ng ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of plasmid DNA was transfected into each well using Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427). Unless otherwise noted ...
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bioRxiv - Cancer Biology 2019Quote: ... Bacterial cells were pelleted by centrifugation at 6,000 RCF at 4°C for 15 minutes and plasmid DNA was extracted using a Qiagen Plasmid Plus Midi Kit (Qiagen #12943). To estimate the total number of unique barcodes present in the plasmid pool ...
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bioRxiv - Physiology 2020Quote: ... RNA was isolated from maternal and fetal tissues (Table 3 and 4) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...