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Citations for Qiagen :
501 - 550 of 807 citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Lysed cells were sedimented by centrifugation at 20,000 RCF at 4 °C for 30 min and the lysate was subsequently loaded onto pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The cell pellet was removed after centrifugation (16000 rpm, 4 °C, 1 h) and supernatant was loaded into a Ni-NTA agarose column (Qiagen). Protein was eluted in an elution buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from the cells dissociated with C&H or dispase or from fresh frozen tissues using RNeasy plus mini kit (#74134, Qiagen). 500 ng of RNA from each sample was used for cDNA synthesis using High-capacity RNA-to-cDNA kit (#4387406 ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
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bioRxiv - Genomics 2024Quote: ... were heat killed in a 95°C water bath for 20 min to produce thermolysates before DNA extraction (MagAttract kit, Qiagen) and whole-genome sequencing ...
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bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated at 37 °C for 30 min followed by immediate purification of DNA fragments with the MinElute PCR Purification Kit (Qiagen). ATAC-seq including amplification of Library and Indexing was performed as described elsewhere67 ...
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bioRxiv - Molecular Biology 2024Quote: ... The swabs were removed after heat incubation at 56 °C for 2 hours by transferring samples including swab material to QIAshredder tubes (Qiagen) and centrifugation at 12,000 rcf for 2 min.
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bioRxiv - Microbiology 2024Quote: ... Lysates were centrifuged for 30 min at 30000 x g in 4 °C and the resulting supernatants were gently mixed with Ni-NTA Agarose beads (Qiagen) pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... 140 μL virus supernatant was treated with 0.25 μg RNaseA for 30 min in 37 °C to remove the extracellular RNA and continued with viral RNA extraction (QIAamp Viral RNA Kits, Qiagen) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 140 μL virus supernatant was treated with 0.25 μg RNaseA for 30 min in 37 °C and continued with viral RNA extraction (QIAamp Viral RNA Kits, Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fecal pellets were collected and stored at −80°C until DNA extraction with DNeasy PowerLyzer PowerSoil Kit (Qiagen; 12855-100). Fecal Rg abundance was determined with Rg-specific 16S rDNA primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
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bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from 23 ethanol-preserved specimens using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany), and from four formalin-fixed specimens using the QIAamp DNA FFPE Tissue Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... reads were subsequently mapped to the reference emm3 genome (MGAS10870; Accession number CP067090.1) using CLC Genomics Workbench version 23 (Qiagen). Identification of significantly differentially expressed genes was determined as we have previously described (M ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria was split across six tubes and each recovered in 2 mL of SOC for 1 h at 37 °C then added to 500 mL of LB with 100 μg/mL of carbenicillin and grown for 9 h at 37 °C prior to plasmid purification (Qiagen, 12991). The plasmid prep was then normalized to 1 μg/μL to generate the final mpra:minP:gfp library used for transfection delivery.
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bioRxiv - Developmental Biology 2021Quote: ... Luciferase reporter constructs were either mock-treated or methylated in vitro with SssI CpG methyltransferase for 4 h at 37 °C and purified with the QIAquick Purification Kit (QIAGEN 28704). Reporter plasmid (500 ng ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The reaction was incubated at 37°C for 30 min and purified using a MinElute Reaction Cleanup Kit (Qiagen, Germantown, MD). The PCR reaction consisted of 30 μl transposed DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Bacterial cells were pelleted by centrifugation at 6,000 RCF at 4°C for 15 minutes and plasmid DNA was extracted using a Qiagen Plasmid Plus Midi Kit (Qiagen #12943). To estimate the total number of unique barcodes present in the plasmid pool ...
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bioRxiv - Plant Biology 2019Quote: ... the pellet was dissolved at 50 °C for 15 minutes in 19 ml of Buffer G2 (Qiagen, Germantown, MD, Cat #1014636).
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bioRxiv - Genomics 2019Quote: ... The fresh blood sample was immediately brought to the laboratory at 4 °C and genomic DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen, USA) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... All transfections were performed in D- or C-medium by using HiPerFect Transfection Reagent according to manufacturer’s instructions (301704, Qiagen, Hilden, Germany). After transfection for 24 hours ...
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bioRxiv - Genetics 2021Quote: ... incubated overnight at 37°C in L-broth containing 50 ng/μL ampicillin and DNA isolated using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C) an applied to a 2 ml Ni-NTA immobilised metal affinity chromatography (IMAC) gravity flow column (QIAGEN, Hilden, Germany). Columns were washed in 10 column volumes (CV ...
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bioRxiv - Cell Biology 2021Quote: ... treated with DNase I at 37°C for 30 min and finally cleaned with the miniElute kit (QIAGEN, Catalog Number: &28004). For next-generation sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: We homogenized tongue or ear tissue (stored at −20°C) and extracted genomic DNA using QIAamp Fast Tissue kits (Qiagen, Inc), with verification via gel electrophoresis (2% agarose) ...
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bioRxiv - Genomics 2021Quote: ... The resultant nuclei were then incubated for 30 min at 37°C with an in-house made Tn5 enzyme and purified with the Qiagen MinElute kit (Qiagen, 28004). PCR reactions for each replicate were performed with 15 cycles using Ad1F and Ad2.2R primers and NEBNext High-Fidelity 2X PCR Master Mix (New England Labs Cat #M0541) ...
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bioRxiv - Microbiology 2019Quote: ... 1 µM of forward and reverse oligos were heated for one minute at 100°C with 5 mM MgCl2 and 7 mM Tris-Cl (i.e. Qiagen Elution Buffer) and annealed by slowly cooling to room temperature.
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bioRxiv - Molecular Biology 2021Quote: ... the PCR products were held at 4°C until PCR purification using the QIAquick PCR purification kit (#28106, Qiagen, Hilden, Germany). DNA concentrations were determined using NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Genomics 2022Quote: ... The resulting pellet was gently resuspended in 20 ml of pre-warmed (37°C) G2 lysis buffer (Qiagen; Cat. no. 1014636), incubated with 50 μg/ml RNaseA (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... harvested and stored at −80°C in RLT buffer with β-mercaptoethanol from the RNeasy Mini prep Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions until cDNA synthesis for RT2 Profiler PCR array ...
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bioRxiv - Microbiology 2021Quote: ... Fecal pellets were snapfrozen and stored at −80°C until DNA extraction with the DNeasy Powersoil kit by Qiagen (Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Transposition was performed for 30 minutes at 37°C after which the transposed DNA was purified using MinElute PCR Purification Kit (Qiagen, 28004) and eluted in 10 µl of elution buffer (EB ...
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bioRxiv - Neuroscience 2020Quote: ... 100μL chloroform were added and samples spun at 7000rpm for 15min at +4°C in a MaXtract column (Qiagen, Venlo, Netherlands). Mixed with 500μL 70% EtOH and spun at 10 000rpm for 30s at +4°C in a MiniElute column ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Microbiology 2019Quote: ... the supernatant was removed with a micropipet and 350 µL of 4°C Buffer RLT Plus (from Qiagen RNeasy Mini Kit) was added to the pellet to collect the RNA ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2020Quote: ... Dehydrated samples were stored at −80 °C until resuspension in 25 μl or 100 μl of Buffer EB (QIAGEN, Venlo, Netherlands).
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bioRxiv - Synthetic Biology 2020Quote: ... and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN, Germany) equilibrated with purification buffer A ...
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bioRxiv - Immunology 2020Quote: ... Pellets were lysed by vortexing for 1 minute in 350uL cold supplemented RLT buffer (RLT + β-MeOH) at 4°C and lysates homogenized using QIAshredder columns (Qiagen). RNA was then extracted from these samples using the RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80°C until clean up using a kit (Qiagen 154025593). cDNA was generated using 11 rounds of amplification with 10 ng RNA input.
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bioRxiv - Physiology 2019Quote: ... Methylation status of the individual CpG sites was identified as an artificial C/T SNP using QCpG software (Pyrosequencing, Qiagen, UK). Then % methylation of each CpG site was then calculated as a proportion of the methylated alleles divided by the sum of all methylated and unmethylated alleles ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80 °C until clean up using a kit (Qiagen 74004). cDNA was generated using 11 rounds of amplification with 10 ng RNA input ...
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bioRxiv - Cell Biology 2020Quote: Isolated Chromatin was digested with 1% proteinase K for 2 hrs at 60 °C with 300 rpm and was subsequently purified by using QIAquick® Nucleotide Removal Kit (28306, Qiagen) according to the manufactory’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged at 5000 g for 2 hours at 4 °C and the pellet was collected for DNA extraction with a DNeasy PowerSoil kit (Qiagen, Germany). The sediment from Lime Blue was collected with a freeze core (modified from Stocker and Williams ...
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bioRxiv - Bioengineering 2022Quote: ... after which the cells were FACS sorted for high and low reporter signal.The genomic DNA was extracted by lysing the cell pellets for 10 minutes at 56°C in AL buffer (Qiagen, 19075), supplemented with Proteinase K (Invitrogen ...