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Citations for Qiagen :
151 - 200 of 1738 citations for ADAM Metallopeptidase With Thrombospondin Type 1 Motif 9 ADAMTS9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from wild type and auts2ancb104 heterozygote and homozygote embryos using an RNeasy Mini kit (QIAGEN), and first-strand cDNA was synthesized from 1 ⎧g of total RNA by oligo(dT ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from validated clones and wild-type HAP1 cells using the RNeasy Mini Kit (Qiagen cat # 74104) following the manufacturer’s instructions and then treated with TURBO DNA-free™ Kit (Life Technologies cat # AM1907 ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with wild-type CrPV or CrPV-DcDV RNA using the TransMessenger transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and the transfected cells were incubated at 26 °C ...
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bioRxiv - Genetics 2020Quote: ... and cells treated with transfection mix without siRNA (Wild type (WT)) or non-targeting control siRNA (scrambled (SCR)) (Qiagen catalog SI03650318 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA and RNA were extracted from the B14804-/- and wild-type cells using AllPrep DNA/RNA Mini kit (Qiagen). cDNA was synthesized from extracted RNA using SuperScript™ III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... rdr1;2;6 Cen5-ATHILA5 ddm1 (hp5) and their corresponding DDM1 wild-type siblings with the DNeasy Plant Pro Kit (Qiagen). From each sample ...
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bioRxiv - Microbiology 2023Quote: ... oxydans wild-type cultures at OD600 0.3 units (Exp) or 3 units (Sta) using the RNeasy Mini Kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... allowing resolution of a 230-bp product for the wild-type allele and a 194-bp product for the mutant allele (DNeasy 96 Blood and Tissue Kit, Qiagen).
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bioRxiv - Genomics 2022Quote: ... The genomic DNA of the wild-type and the mutant strains was isolated using a QIAamp DNA Mini Kit (QIAGEN). Libraries were prepared using the Nextera XT DNA Library preparation kit (Illumina) ...
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bioRxiv - Genomics 2020Quote: Thirty-three RNA samples were extracted from three biological replicates of each of 11 diverse tissue types of A188 with RNeasy Plant Mini Kit (Qiagen) (Supplementary Table 10) ...
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bioRxiv - Developmental Biology 2020Quote: ... ∼100 ng of total RNA from each cell type was used as input to generate miRNA sequencing libraries using a QIAseq miRNA library kit (Qiagen). Single-end 100 bp reads (1×100 ...
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bioRxiv - Immunology 2021Quote: ... cDNA was quantified using 96-well PCR array analysis on a PAMM-150ZA plate (Cytokines & Chemokines) and PAMM-016ZA plate (Type I Interferon Response) (both Qiagen). Quantitative real time-PCR (QRT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from 5-day-old wild-type and Mpatg mutant thalli using the RNeasy Plant Mini Kit (Qiagen) and used as a template for reverse transcription using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ES2 human clear cell carcinoma cells present (wild type) or absent (siRNA) ARID1A were collected and extracted using an RNeasy Plus Micro Kit (Qiagen). Barcoded TruSeq RNA v2 libraries (Illumina ...
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bioRxiv - Genetics 2022Quote: ... DNA was extracted from a wild type clone and a mutant using a QIAGEN Plasmid Midi Kit (Qiagen, Tokyo, Japan) and used as standard DNA to determine ratios of mosaicism ...
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bioRxiv - Cancer Biology 2022Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA)(42) (Qiagen) and Genomic Regions Enrichment of Annotations Tool (GREAT)(43) ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from 20 trunks of either E8.5 wild-type or Aldh1a2-/- embryos with the RNeasy Micro Kit (Qiagen #74004). Reverse transcription was performed with the High-Capacity cDNA RT Kit (Thermo Fisher Scientific #4368814) ...
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bioRxiv - Genomics 2019Quote: ... and two control bulls that were homozygous for the wild type allele was extracted from testis tissues using the RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNAs were extracted from wild-type and Cables2-deficient EpiLCs at 2 days post-induction (n = 3) using RNeasy Plus Mini Kit (Qiagen). RNA quality was evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies Japan ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from wild-type and ΔTMPRSS2 293T cell lines using the Puregene cell kit according to the manufacturer’s instructions (Qiagen, #158388). Co-transfection of pJC144 (guide 1 ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from cultured tprAko-SS14 or wild-type strains propagated in 6-well plates following the transformation procedure was performed using the QIAamp mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: E9.5 hindbrain spanning rhombomeres 1-6 were dissected from wild type and mutant embryos (n=3) to obtain total mRNA preparations (miRNeasy Micro Kit, Qiagen). Sequencing libraries were prepared following the SMART-Seq v4 Ultra Low Input RNA (TaKaRa ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from one wild-type control and three patient cell lines using DNeasy Blood and Tissue kit (Qiagen). Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... with tissue homogenization being performed using a rotor stator type tissue homogenizer (ProScientific Bio-Gen PRO200 Homogenizer; Multi-Gen 7XL Generator Probes) in RLT Buffer (Qiagen). RNA quality was assayed using a nanodrop ...
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bioRxiv - Neuroscience 2022Quote: Brain and spinal cord tissue from Prp-TDP43A315T (Tg) animals and wild type (Wt) littermates were homogenized using a TissueRuptor (Qiagen). 1 mL of TRIzol per 250 mg of tissue was used for homogenization and RNA was isolated as per the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: Two-week-old Arabidopsis seedlings of Col-0 wild type and trb1/2/3 triple mutans were used for DNA extraction using DNeasy Plant Mini Kit (QIAGEN). A total of 500 ng DNA was sheared with Covaris S2 (Covaris ...
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bioRxiv - Molecular Biology 2023Quote: ... cf-DNA quantified from various plasma or serum types using either MitoQuicLy or a silica-based membrane column DNA extraction kits (DNeasy, Qiagen). A total of 50 samples were analyzed (5 biofluids ...
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bioRxiv - Biochemistry 2023Quote: ... A linearized PCR template was generated by digesting a wild-type Hrd1 plasmid with restriction enzyme and purified using a QIAquick PCR purification kit (Qiagen). For each region two separate PCR reactions were set up ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from adult ovaries of transgenic or wild-type zebrafish using the RNeasy Mini kit (QIAGEN, 74134) and reverse-transcribed using SuperScript IV reverse transcriptase (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from HeLa cells transfected with either wild-type PADI2 (WT) or mutant PADI2 (with L642A, or L642A/W161A, or L642A/W161A/T159A) was extracted using RNeasy (Qiagen) according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA) (Qiagen) (90).
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bioRxiv - Molecular Biology 2024Quote: RNA isolation from leaf tissues of five-week-old non-transformed wild type and overexpressing transgenic lines (two replicates of wild type and two transgenic lines) were isolated as per manufacturer’s instruction (Qiagen, Germany). High quality RNA samples with 200 ng/μL concentration were outsourced for RNA-seq ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from two-week-old Villersexel wild type and the nog1dis mutant using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Microbiology 2019Quote: ... was used to amplify species specific 16S rRNA genes in order to identify and to quantify the relative abundance of 45 types of gut microbiota according to the manufacturer’s instructions (Qiagen, Hilden Germany) (Supplementary table 1) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the allantoic fluid of recombinant and wild type after the first and fifth passages in eggs using the QIAamp® Viral RNA Mini Kit (Qiagen). The real-time qRT-PCR was performed using SuperScript™ III Platinum™ One-Step qRT-PCR Kit to detect NDV M gene41 ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from the fifth leaf of wild-type and mutant plants using the RNeasy Plant Mini Kit (Qiagen, Germany). First-strand cDNA was synthesized from 500 ng total RNA using the PrimeScript RT reagent kit (Takara ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from transgenic seedlings carrying 35S promoter-driven wild-type RAPTOR1B/ raptor1b or RAPTOR1BS916A/raptor1b and control seedlings using a Qiagen RNeasy kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Genomic DNA was isolated from wild type (mCherry negative) and mCherry positive cells using Qiagen DNeasy Blood & Tissue Kit (Qiagen N.V.). DNA concentration was measured and calculations were made to prepare a genomic DNA mixture representing approximately 100 transduced cells and 3.79×104 wild type cells in a total of 250 ng DNA corresponding to approximately 3.8×104 cells ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from NPCs and their derived astrocytes (4 lines, n=3 per cell type) using a RNeasy mini kit (Qiagen, 74104). RNA samples were prepped using TruSeq® Stranded mRNA Library kit (Illumina ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from frozen mammary tissue from wild-type mice at day six of pregnancy and purified with RNeasy Plus Mini Kit (Qiagen, 74134). Ribosomal RNA was removed from 1 μg of total RNAs and cDNA was synthesized using SuperScript III (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... TPOX and FGA) and the Amelogenin (AMEL) gender-identification locus (24,25) using the Type-it Microsatellite PCR kit (Qiagen Gmbh, Hilden). The three multiplex PCRs and their respective primer pairs are given in Supplementary Table 1 ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from frozen mammary tissue from wild-type mice at day six of pregnancy and purified with RNeasy Plus Mini Kit (Qiagen, 74134). Ribosomal RNA was removed from 1 μg of total RNAs and cDNA was synthesized using SuperScript III (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from wild type and RFX2 -/- LUHMES cells and column-purified using an RNeasy mini kit (Qiagen 74104) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The complete set of 15 loci were amplified in four separate 15 μL multiplex-PCR reactions containing 7.5 μL of 2X Master Mix (Type-It Microsatellite PCR kit, Qiagen, Venlo, Netherlands), 0.015-0.15 μL of unlabeled forward primer with tail (10 μM) ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from whole frozen renal tissue from wild-type and mutant mice and purified with RNeasy Plus Mini Kit (Qiagen, 74134). Ribosomal RNA was removed from 1 μg of total RNAs ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA of wild-type and KO mice was prepared from their tails using the DNeasy Blood & Tissue Kit (QIAGEN, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 katna1-/-MZ and one pool of 10 wild-type embryos (at 24 hpf) using the RNeasy Mini-kit (Qiagen, France) and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen ...