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Citations for Qiagen :
101 - 150 of 1738 citations for ADAM Metallopeptidase With Thrombospondin Type 1 Motif 9 ADAMTS9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... Dmdmdx-βgeo and corresponding wild-type myoblasts using RNEasy Plus Universal mini kits (Qiagen 73404). Briefly ...
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bioRxiv - Genetics 2023Quote: ... Matched blood and hair samples were amplified using the Type-it Microsatellite PCR kit (Qiagen) in a 25-μl volume containing 12.5 μl of 2 x Type-It Master Mix ...
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bioRxiv - Genetics 2024Quote: ... total RNA was purified from various stages of wild-type using the RNeasy system (Qiagen) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Neuroscience 2024Quote: Total DNA from all cell types was isolated using the DNeasy Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted form two-week-old wild-type tissue using an RNeasy kit (Qiagen) and treated with Turbo DNase (Ambion) ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria was split across six tubes and each recovered in 2 mL of SOC for 1 h at 37 °C then added to 500 mL of LB with 100 μg/mL of carbenicillin and grown for 9 h at 37 °C prior to plasmid purification (Qiagen, 12991). The plasmid prep was then normalized to 1 μg/μL to generate the final mpra:minP:gfp library used for transfection delivery.
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bioRxiv - Genomics 2022Quote: ... equal volumes of the clones were combined together and the pool expanded in 20 mL of LB with 100 μg/mL carbenicillin for 9 hr followed by plasmid purification (Qiagen, 12943). Approximately 250 colonies in total were harvested.
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bioRxiv - Biochemistry 2021Quote: Purified CrFBA3 was tested for crystallization at two concentration of 9 mg/ml and 4.5 mg/ml on commercial sparse-screening conditions (Qiagen, Hilden Germany) based on the work of Jancarik and Kim (Jancarik ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
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bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 9 AAA and 10 PAD patients’ serum using the miRNeasy Serum/Plasma Advanced Kit (Qiagen, Hilden, Germany), following in principle the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Immunology 2021Quote: ... the RT2 Profiler Array “Type I Interferon Response” (Cat. No. 330231 PAMM-016ZA) (Qiagen, Hilden, Germany) was used ...
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bioRxiv - Genetics 2020Quote: ... Primer design was optimised to work with the Type-it Microsatellite PCR kit (QIAGEN, Hilden, Germany).
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bioRxiv - Microbiology 2021Quote: ... The PCR mixture contained 1x master mix for the Type-it Microsatellite PCR Kit (Qiagen, France), 3 µL of Q-Solution and 0.25 µM of each primer in a total volume of 30 µL ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was isolated from wild-type and fap92 cells using RNeasy Plus Mini Kit (Qiagen). cDNA synthesis was performed using SuperScrip IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... RNA from collected cell types and (cancer) cell lines was isolated using the miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Each reaction contained 5 μL DNA solution and PCR mixture with Taq Type-it (Qiagen®). PCR products were analyzed by capillarity electrophoresis on an ABl3130xl sequencer ...
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bioRxiv - Physiology 2020Quote: ... existing media was removed and the resulting myotubes received either 750 µl of fresh DM alone or 750 µl DM containing 9 µl HiPerFectTransfection™ (Qiagen, UK) and 20 nmol Flexitube GeneSolutions™ (siUBR5 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were co-transfected with the 9 × GCCG-reporter construct and the renilla-luciferase control vector using SuperFect (Qiagen, Valencia, CA) for 24 h ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was isolated from all Arabidopsis genotypes at 6 and 9 days after inoculation using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted and purified as described in [9] using the Quick-RNA MiniPrep Kit (Zymoresearch, Irvine, CA, USA) and RNeasy MinElute Cleanup Kit (QIAGEN, Hilden, Germany). Per sample at least 150 ng of high-quality RNA were obtained ...
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bioRxiv - Microbiology 2019Quote: ... and the variants (single nucleotide changes and small deletion up to 50 nucleotides) were detected with the Fixed Ploidy Variant Detection tool in CLC Genomics Workbench version 9 (CLC, Bio-QIAGEN, Aarhus, Denmark). Variants falling in PE/PPE family genes were excluded from the analysis.
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bioRxiv - Developmental Biology 2020Quote: ... and unfragmented genomic DNA (A260/A280 ≥ 1.8 and A260/A230 ≥ 1.9) was extracted from whole blood obtained from the subject and his parents using the Puregene Blood kit from Qiagen (Valencia, CA). Whole exome sequencing was performed using the service provided by Beijing Genomics Institute (Cambridge ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed once with 0.1% PBSA and incubated with 50 μL of a 1:100 dilution of anti-penta-His Alexa 647 antibody (Qiagen) for 10 minutes on ice ...
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bioRxiv - Biochemistry 2020Quote: ... but with an anti-His antibody (Qiagen, #34660 at a dilution of 1:5000) as primary ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Molecular Biology 2020Quote: Total RNAs were extracted from wild-type or F14R HAP1 with the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Total mRNA was isolated from OregonR or Dijon2000 wild-type stage 17 embryos (RNeasy Micro Kit, Qiagen), and total cDNA was prepared (Superscript III First-Strand Synthesis System ...
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bioRxiv - Plant Biology 2019Quote: ... total RNA was extracted from wild-type and mutant nodules using the RNeasy Mini kit (QIAGEN, Germany) and treated with DNase (Turbo DNase kit ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from wild-type mESCs and Pml KOs using RNeasy Mini Kit (QIAGEN, 74104) and treated with Turbo-DNase (Ambion ...
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bioRxiv - Genetics 2019Quote: RNA was extracted from PCYT1A-wild-type human fibroblasts using the RNeasy Mini Kit (Qiagen, Cat#74104), reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen ...
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bioRxiv - Genomics 2023Quote: The PCR was performed with the Multiplex PCR Kit (Type-It Microsatellite master mix; Qiagen, Hilden, Germany). The SSR primer mix consisted of eight primers (see Table 2 and Table 3) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the microsatellite loci were PCR[amplified in five separate multiplexed reactions using a Type It Kit (Qiagen). Fluorescently labelled PCR products were then resolved by electrophoresis on an ABI 3730xl capillary sequencer (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: Data to derive different biological clocks was available for different subsamples and all based on a fasting blood draw from participants in the morning between 8:30 and 9:30 after which samples were stored in a −80°C freezer or – for RNA - transferred into PAXgene tubes (Qiagen, Valencia, California, USA) and stored at −20°C ...
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bioRxiv - Genetics 2022Quote: ... and conventional extraction methods (n = 9) using a lysis buffer and purified using silica columns (Qiagen DNeasy Plant Kit Qiagen Inc, Hilden, Germany). All lysates were analyzed with a QubitTM 4 Fluorometer (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated for 1 hour with monoclonal α-His antibodies conjugated to the horseradish peroxidase (1:5000 dilution; Qiagen). Blots were visualized with the addition of Lumina Forte Western HRP Substrate (MilliporeSigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... The PCR mix (final volume of 15.75 µL) consisted of: 7.5 µL Type-it Multiplex PCR Master Mix (QIAGEN), 3 µL Q solution (5X) ...
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bioRxiv - Cancer Biology 2019Quote: The cDNA of wild-type ecATCase and regulatory chain of ecATCase-holo were amplified by PCR (Qiagen Kit) using BL21(DE3 ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA from wild type (WT) and YBX1 KO cells was extracted using DNeasy Blood & Tissue Kit (Qiagen) and amplified using the primers 5’-GGGTAATGAGGCTACAACTGTTT-3’ and 5’-GTCAACTCTAACACATTTCCACGTAT-3’ ...
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bioRxiv - Microbiology 2021Quote: Total RNA extraction was performed from the wild-type and mutant clones using RNeasy plus mini kit (QIAGEN). RNA was quantified by qubit 4 ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenised to a fine powder using a ball mill-type grinder (Tissuelyser II Qiagen, 85300). For the homogenization of the frozen material one liquid nitrogen cooled 5 mm stainless steel metal balls was added to each Eppendorf tube and the frozen material was disintegrated for 1 min at 25 Hz.
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bioRxiv - Genomics 2023Quote: DNA from both types of samples was extracted using a commercially available kit (DNeasy Blood & Tissue Kit, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Immunology 2021Quote: The indicated cell types were harvested and stored at −80°C until RNA was isolated using RNeasy kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: dram1 or dram1Δ19N (negative control) RNA was isolated from wild type or dram1Δ19n/Δ19n embryos using QIAzol lysis reagent (79306, QIAGEN) and purified with the RNeasy MinElute Cleanup kit (74204 ...