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Citations for Qiagen :
501 - 550 of 1933 citations for 6H Pyrano 3 2 g benzothiazole 8CI 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... 3 mL of culture were mixed with 6 mL of RNAprotect bacteria reagent (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the media was collected and applied to 3 ml of Ni-NTA resin (Qiagen). The resin was collected and washed with PBS supplemented with 20 mM imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... 0.001 M of HCl at pH 3 was added and then homogenized (TissueRuptor, Qiagen) on ice ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were reverse transfected with 5 pmol of each siRNA (QIAGEN) using lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... cells or tissues were lysed using a TissueLyser II (3 min, 30 Hz; Qiagen) in RIPA buffer (150 mM NaCl (Merck) ...
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bioRxiv - Plant Biology 2021Quote: Plant tissue was disrupted mechanically using 3 mm tungsten carbide beads (Qiagen, Hilden, Germany), and a TissueLyser II (Quiagen ...
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bioRxiv - Immunology 2019Quote: ... 3 times and then eluted with 350 uL of buffer RLT (Qiagen RNeasy kit). Input and immunoprecipitated RNA was purified on column (Qiagen RNeasy kit ...
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bioRxiv - Plant Biology 2022Quote: ... from Brachypodium Bd21-3 genomic DNA extracted using the DNeasy Plant Mini Kit (Qiagen) or specific plasmids using primers that contained Type IIS restriction sites and overhangs complementary to the respective entry module ...
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bioRxiv - Microbiology 2023Quote: ... contaminated DNA was digested by 3 times treatment with RNase-Free DNase Set (Qiagen), and the absence of DNA contamination in extracted RNA was confirmed by PCR.
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from six hundred testes of ≤3 days old using miRNeasy (QIAGEN). Less than 200 nt fragments were collected using miRelute column (QIAGEN) ...
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bioRxiv - Cell Biology 2023Quote: ... pools of 3 x 103 MPP1-4 were sorted directly into RLT buffer (Qiagen) enriched with 1% of β-mercaptoethanol ...
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bioRxiv - Genetics 2023Quote: ... and a single 3 mm metal bead (Eldan, BL6693003000) using TissueLyzer LT (QIAGEN, #85600) with a dedicated adaptor (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... for 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). Vero cells were seeded in 24-well plates and incubated for 24 hours to reach confluency ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were sorted into 384 well plates each containing 3 μl Vapor-Lock (Qiagen) in all wells and eight different conditions of 0.3 μl lysis buffer consisting of 0.125μM OligodT30VN (5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’ ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Biophysics 2019Quote: ... Lysates were spun for a further 20 min at 40,000 g at 4 °C and resulting supernatants were incubated 1 hour with Ni2+-nitrilotriacetate (NTA) agarose beads (QIAGEN) at 4 °C on rotating wheel ...
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bioRxiv - Genomics 2022Quote: ... Multiple displacement amplification (MDA) was carried out using the REPLI-g Single Cell Kit following the manufacturer’s recommendations with slight modifications (Qiagen). MDA reactions were incubated at 30°C for 16 hr ...
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bioRxiv - Genomics 2020Quote: ... The worm pellet was flash frozen and genomic DNA was isolated using the Genomic-tip 100/G column (Qiagen). Four micro-grams (average size 23Kb ...
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bioRxiv - Bioengineering 2020Quote: ... 0.25 g of sand or soil was sampled from each surface and processed using a DNeasy Powersoil kit (Qiagen) to isolate DNA ...
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bioRxiv - Genomics 2021Quote: ... in a 15 ul reaction volume in 96-well plates or with the Repli-g Single Cell kit (Qiagen) in a 10 ul reaction volume in 384-well plates ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 μg of mHTTex1 seed or 50 μg of seeded neuronal lysate was incubated with varying doses of proteinase K (0 to 0.5 μg/mL, Qiagen) for 30 min at 25°C and heat inactivated at 75°C for 10 min ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Biochemistry 2020Quote: ... The solubilized membranes were centrifuged in a Sorvall WX ultracentrifuge (~109,000 g; 45 min, F50L-8×39 rotor) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Microbiology 2020Quote: Whole metagenome amplification was performed using multiple displacement amplification (MDA) with the REPLI-g Single Cell kit (Qiagen, 150345). MDA was performed using DNA from environmental samples and controls for each day ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by 5 minutes of centrifugation at 16.000 g of bacterial lysate was stored at −80 °C or immediately purified with the miRNeasy mini kit (Qiagen). RNA quantity was determined using the Qubit 4 Fluorometer and Nanodrop spectrophotometer (Thermofisher) ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA was reverse-transcribed then isothermally amplified using a REPLI-g WTA Single Cell kit (Qiagen, Hilden, Germany) as previously described (68) ...
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 14,000 × g for 10 min and RNA was extracted using Qiagen RNeasy mini plus kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by ultracentrifugation (70,000 × g, 30 min) and supernatant was incubated with Ni-NTA resin (Qiagen) for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Immunology 2022Quote: Bacterial genomic DNA was isolated using the Blood and Cell Culture DNA kit and 500/G genomic tips (Qiagen) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins and bacterial membranes were separated by centrifugation (30 min, at 15,000 ×g) and the supernatant was applied to Ni-NTA beads (Qiagen) for 2h ...
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bioRxiv - Microbiology 2020Quote: ... The homogenates were clarified by centrifugation at 9,000 × g for 5 min and RNA was extracted using RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... Six replicate 0.35 g extractions were combined and re-purified with the MO BIO PowerClean Pro kit (QIAGEN, Germany) and eluted in 50 mL of 10 mM Tris buffer.
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from the 0.25 g of soil from the resulting pellet using the DNeasy PowerSoil Kit (Qiagen). DNA extractions were verified by gel electrophoresis in a 1% agar gel.
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The mitochondrial genome of the red icefish (Channichthys rugosus) casts doubt on its species statusbioRxiv - Zoology 2022Quote: ... the lysates were centrifuged at 17,000 x g for 5 minutes and 300 μL supernatant mixed with 3000 μL Buffer PB (Qiagen). The mixtures were loaded onto Minelute silica columns (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... Debris was removed by centrifugation (20,000×g, 30 min, 4°C) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA from Cas9 control and OCT4-targeted was isolated using the REPLI-g Single Cell Kit (QIAGEN, 150343). DNA samples isolated for cytogenetic analysis were amplified with the SurePlex Kit (Rubicon Genomics) ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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Germline Selection by Meiosis Defends the Transmission of defective Mitochondria with mtDNA variantsbioRxiv - Cell Biology 2020Quote: ... Then each PB1 sample was amplified with whole genome amplification (WGA) using REPLI-g Single Cell Kit (QIAGEN, 150345). For each oocyte sample ...
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bioRxiv - Microbiology 2020Quote: ... and IMX2137 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were centrifuged for 30 min at 45,000 × g and incubated with 1 ml of Ni-NTA agarose (Qiagen) per 1 l expression culture ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was isolated by centrifugation for 30 min at 20,000 ×g and then incubated with a Ni2+-NTA column (QIAGEN) for 5h ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris and unlysed cells were pelleted at 48,000 × g and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...