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Citations for Qiagen :
51 - 100 of 857 citations for 5α CHOLESTAN 3 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... cDNAs were synthesized using one-step RT-PCR kit (Qiagen; 205311). To amplify specific cDNAs ...
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bioRxiv - Immunology 2019Quote: ... one tissue segment was immediately preserved in RNAlater (Qiagen, Valencia, CA) for RNA extraction ...
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bioRxiv - Physiology 2021Quote: One lobe of lung tissue was homogenized by a TissueLyser (Qiagen) in Trizol solution (Life Technology) ...
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bioRxiv - Developmental Biology 2021Quote: ... one gonad with mesenephros was removed and kept in RNAlater (Qiagen) at -20°C until RNA extraction ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using One Step RT-PCR kits (Qiagen) and a mixture of TRAV- ...
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bioRxiv - Immunology 2019Quote: ... The cells were cultured for one day before isolating RNA (Qiagen) as previously described ...
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bioRxiv - Immunology 2019Quote: ... The cells were cultured for one day before isolating RNA (Qiagen) as previously described ...
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bioRxiv - Molecular Biology 2023Quote: ... and analysed using a One Step RT-PCR kit (Qiagen, #210210) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... on a 5-plex QIAcuity One digital PCR instrument (Qiagen, 911021). The thermal cycling conditions were implemented using the following program ...
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Temporal Dynamics of Neocortical Development in Organotypic Mouse Cultures: A Comprehensive AnalysisbioRxiv - Neuroscience 2024Quote: ... Tissue was homogenized by addition of one metal bead (steel, Qiagen) and 1 ml trizole (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: siRNAs used were as follows: Kif5b#3 (target sequence 5′-CAGCAAGAAGTAGACCGGATA-3′; Qiagen SI00176050), Kif5b#4 (target sequence 5′-CACGAGCTCACGGTTATGCAA-3′ ...
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bioRxiv - Cell Biology 2021Quote: ... siMad2 (5’-CUGAAAGUAACUCAUAAUCUA -3’) (Qiagen). For transfection of the scFv or scFvC plasmids ...
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bioRxiv - Physiology 2020Quote: ... A one-step PCR kit (QuantiFast™ SYBR® Green, Qiagen, UK) was used to assess gene expression ...
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bioRxiv - Plant Biology 2020Quote: ... Amplification was carried out using a One-Step RT-PCR Kit (QIAGEN) and BIO-RAD T100 Thermal Cycler ...
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bioRxiv - Molecular Biology 2019Quote: RT-PCRs were performed with the One-step RT-PCR kit (QIAGEN) with SAO00187/SAO00188 (for 12S rRNA) ...
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bioRxiv - Molecular Biology 2022Quote: ... one half of each cleanup prepared using MinElute PCR Purification Kit (QIAGEN) was treated with a cocktail of exonucleases comprising 0.2 U/μL T7 exonuclease ...
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bioRxiv - Immunology 2022Quote: ... One part was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... All assays were adapted to the One-step RT-PCR Kit (Qiagen) reaction chemistry ...
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bioRxiv - Neuroscience 2022Quote: ... one 5 mm bead per sample was used in a TissueLyser (Qiagen) for 4 min at 30 Hz ...
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bioRxiv - Evolutionary Biology 2022Quote: ... At least one specimen was placed in RNAlater solution (Qiagen, Hilden, Germany) for RNA preservation and frozen at −80 C within one week of collection to prevent RNA degradation ...
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bioRxiv - Molecular Biology 2023Quote: ... and one volume isopropanol before application to a Qiagen quickspin column (Qiagen) and elution according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... on a 5-plex QIAcuity One digital PCR instrument (911021, Qiagen, USA). The thermal cycling conditions were implemented using the following program ...
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bioRxiv - Genomics 2022Quote: ... One-fifth of the cells was used for plasmid DNA extraction (Qiagen). The remaining cells were used for RNA extraction using the innuPREP RNA Mini Kit 2.0 (Analytik Jena) ...
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bioRxiv - Immunology 2023Quote: ... One biopsy was thawed on ice and homogenized in RLT buffer (Qiagen) employing the TissueLyser LT (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... For one-way qPCR (analysis of MAP3K1, Hs_MAP3K1_1_SG QuantiTect Primer Assay, Qiagen); Luna® Universal One-Step RT-qPCR Kit was used ...
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bioRxiv - Molecular Biology 2023Quote: ... An A base was added to the 3’ ends with Klenow (3’-4’ exo-) (Qiagen). The processed ds-cDNA was then ligated to Illumina sequencing adapters with T4 DNA Ligase (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... NG1 (5’ACCGACCACAGGGGG-3’) and NG2 (5’-GGTTGTAAACCTCTTTCGA-3’) and HotStarTaq® Master Mix Kit (Qiagen) up to a final volume of 25 μL ...
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bioRxiv - Developmental Biology 2019Quote: ... One microgram of mRNA was converted to cDNA using Quantitect reverse transcriptase (Qiagen), and then diluted 1:10 for subsequent qRT-PCR analysis using SYBR Green JumpStart Taq (Sigma ...
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bioRxiv - Microbiology 2019Quote: One µg of cDNA was synthesised using the QuantiTect reverse transcription kit (Qiagen) with the initial genomic wipe-out step included ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from one PBMC sample using the RNeasy mini kit (Qiagen). DNA was extracted from the other PBMC sample using the MagAttract HMW DNA kit (Qiagen).
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bioRxiv - Genomics 2021Quote: ... One million enriched mESCs were lysed and RNA extracted using the RNeasy (QIAGEN) RNA extraction kit ...
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bioRxiv - Neuroscience 2020Quote: ... One metal bead (Peqlab Biotechnologie GmbH, Germany) and 350 μl RLT buffer (Qiagen) including 1% β-mercaptoethanol was added to the frozen tissue parts ...
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bioRxiv - Microbiology 2021Quote: ... the QIAcuity One-Step Viral RT-PCR Kit (Cat No. 1123145, Qiagen, Germany) and the 24-well 26K Nanoplates (Cat No ...
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bioRxiv - Plant Biology 2020Quote: ... Initial amplification was carried out using a One-Step RT-PCR Kit (QIAGEN) in a BIO-RAD T100 Thermal Cycler ...
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bioRxiv - Microbiology 2021Quote: ... The RT-PCR was performed using Qiagen One-Step RT-PCR kit (Qiagen) master mix ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was performed using a One-Step RT-PCR kit (Qiagen, #210210) using the following primers Forward ...
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bioRxiv - Molecular Biology 2021Quote: ... One microgram RNA was reverse transcribed using QuantiTect reverse transcription kit (QIAgen, UK). Ten nanograms of diluted cDNA in qPCR water (Roche ...
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bioRxiv - Immunology 2021Quote: One million cells were resuspended in 600 μl of RLT Buffer (Qiagen; 79216) containing 1% 2-BME and snap frozen in a dry ice – ethanol bath for RNA isolation ...
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bioRxiv - Microbiology 2020Quote: ... and RT-PCR carried out using the One-Step RT-PCR kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2022Quote: ... Primer extension reaction was performed with one unit of Taq polymerase (QIAGEN, Germany), 0.2 mM dNTP mixture ...
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bioRxiv - Immunology 2022Quote: ... One-step qPCR was done with the QuantiNova SYBR Green qPCR Kit (QIAGEN) by using 20 ng RNA from each donor ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated one day later with Gentra Puregene Cell Kit (Qiagen). Amplicons for long-range sequencing were generated with Q5 High-Fidelity polymerase in 100 uL reactions (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... One µl of cDNA was added to SYBR Green PCR Master Mix (Qiagen) and subjected to PCR amplification (one cycle at 95°C for 20 seconds ...
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bioRxiv - Microbiology 2023Quote: ... containing 500 µl PBS and one 5 mm steel bead (Qiagen, Hilden, Germany) prior to bead-beating for 20 seconds to disrupt the cuticle ...
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bioRxiv - Immunology 2024Quote: ... Digital PCR reactions were run using the QIAcuity One Digital PCR System (Qiagen) in a 96-well 8.5k partitions nanoplate ...
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bioRxiv - Microbiology 2024Quote: ... (NM-2) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for DNA extraction of minipig blood and (NM-3 ...
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bioRxiv - Cell Biology 2021Quote: ... si-FAU (5’-CCGGCGCUUUGUCAACGUUGU-3’, Qiagen), si-uS19 (5’-UCACCUACAAGCCCGUAAA-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... si-eS4X (5’-CUGGAGGUGCUAACCUAGGAA-3’, Qiagen), si-FAU (5’-CCGGCGCUUUGUCAACGUUGU-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... #4: 5’-CCGGTTTAGCTGAAGATTCAA-3’ (SI00443779, Qiagen), GTPBP10 siRNA 5’-TTGCGTGTTGTTCAGAAAGTA-3’ (SI04308647 ...
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bioRxiv - Physiology 2022Quote: ... Fifteen milligrams of liver were homogenized in 200 µL of a 3:3:2 solution of acetonitrile:isopropanol:water (MeCN:IPA:H2O) in ceramic bead tubes (1.4 mm, Qiagen #13113-50) using a TissueLyzer II (Qiagen #85300) ...