Labshake search
Citations for Qiagen :
401 - 450 of 10000+ citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... and transfected 24 hours later with 100 nM antisense oligonucleotide targeting miR-194-5p or miR-608 as control (miR-608 is not expressed in Hep G2 cells) (Exiqon, Qiagen) using Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2021Quote: ... PCR was then performed using THUNDERBIRD SYBR qPCR Mix (Toyobo) on a Rotor-Gene Q real-time cycler (Qiagen). The primer set used is listed in Supplementary Table 8 and samples were tested in triplicate per tissue.
-
bioRxiv - Immunology 2021Quote: ... The effects of mRNA silencing by siRNA was investigated by real-time PCR using specific QuantiTect primer Assay (Qiagen).
-
bioRxiv - Immunology 2019Quote: ... RNA collected for real time PCR experiments was further purified using column DNA digestion (RNase-Free DNase set, Qiagen). cDNA was prepared using iScript cDNA-synthesis kit (Invitrogen ...
-
bioRxiv - Cancer Biology 2020Quote: Quantitative real-time PCR for stemness and differentiation genes was performed using primers purchased from Qiagen (QuantiTect primer assay). RNA was isolated from cells according to the manufacturer’s instructions using Trizol (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... the samples were run in the ViiA 7 Real-Time PCR System with the QuantiTect SYBR Green (Qiagen, 204143) (2017) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Transcribed cDNA was diluted 5-fold and used for real-time quantitative PCR (QuantiTect SYBR Green; Qiagen ref: 204145). RNA-sequencing libraries were produced with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (E7770) ...
-
bioRxiv - Plant Biology 2023Quote: ... and 20 ng cDNA were subjected to the Rotor-Gene Q 5-Plex HRM real-time PCR system (Qiagen), using the default program ...
-
bioRxiv - Cancer Biology 2023Quote: ... hsa-miR-29c-3p and hsa-miR-30b-3p were purchased (Qiagen). Karpas1718 cells (1 million per sample ...
-
bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Neuroscience 2021Quote: ... using SYBR-Green based detection system with QuantiNova SYBR green PCR Kit (Qiagen, 208052). Relative expression of mRNA was calculated through ΔΔCt method using the ribosomal protein RP49 RNA expression as internal control for each sample ...
-
bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... and run on the RT^2 First Strand Kit (Stem cell PCR array) (Qiagen). The array was run in triplicate (N=3 ...
-
bioRxiv - Neuroscience 2019Quote: ... All the qPCR experiments were performed using Quantifast SYBR Green RT-PCR Kit (Qiagen) in a LightCycler 480 II System (Roche) ...
-
bioRxiv - Immunology 2019Quote: ... Human RAC2 3’UTR was amplified with One Step Ahead RT-PCR kit (Qiagen) from human mRNA using the following primers RAC2 3’UTR+ ...
-
bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed by using QuantiTect SYBR® Green PCR Kit (#204145, QIAGEN) on Agilent MP3005P thermocycler ...
-
bioRxiv - Microbiology 2019Quote: ... For this purpose we used the QIAGEN OneStep RT-PCR Kit (Qiagen, CA-EUA) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... TER1 was amplified from circularized RNA with the OneStep RT-PCR Kit (Qiagen, 210210) (primer sequences in Table S6) ...
-
bioRxiv - Microbiology 2020Quote: ... HIV-1 RNA was analyzed using the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a LightCycler 480 (Roche) ...
-
bioRxiv - Immunology 2021Quote: ... Viral load was quantified by Taq-Man qPCR using OneStep RT-PCR Kit (Qiagen) and AriaMx Real-time PCR Systems (Agilent) ...
-
bioRxiv - Neuroscience 2020Quote: ... RNA (500 ng) was reverse transcribed into cDNA using QuantiTect RT-PCR kit (Qiagen). RT-PCR was performed on a BioRad CFX384 thermocycler using pre-validated Taqman primers (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen). qPCR was performed using the QuantStudio 7 Flex System ...
-
bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in triplicate using the QuantiNova SYBR Green PCR Kit (Qiagen) and the following primer sequences ...
-
bioRxiv - Developmental Biology 2023Quote: ... and RT-qPCR was performed using the miScript SYBR Green PCR Kit (Qiagen, 218073) together with the miRCURY LNA miRNA PCR Assay (Qiagen ...
-
bioRxiv - Physiology 2023Quote: ... A one-step real-time quantitative polymerase chain reaction (RT-qPCR) was performed using a QuantiFast SYBR Green RT-PCR one-step kit on a Rotorgene 3000Q thermocycler (Qiagen, UK). Each reaction was setup as follows ...
-
bioRxiv - Cancer Biology 2021Quote: ... or miR-22i (Qiagen) for 48 h to up- or down-regulate intracellular miR-22 ...
-
bioRxiv - Immunology 2021Quote: ... Reverse transcription and subsequent PCR amplification (RT-PCR1) were performed using the Qiagen OneStep RT PCR mix (Qiagen). The cDNA was selected and unused primers were removed by SPRIselect bead selection (Beckman Coulter ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR assays were performed based on previous published method (Gonatopoulos-Pournatzis et al., 2018) OneStep RT-PCR kit (QIAGEN) was used according to the manufacturer’s recommendations ...
-
bioRxiv - Genomics 2022Quote: ... cDNA of each gene target was reverse-transcribed and PCR amplified with the PyroMark OneStep RT-PCR kit (QIAGEN) using one biotinylated (reverse ...
-
bioRxiv - Microbiology 2019Quote: ... qRT-PCR to quantify cap and gyr mRNA was performed using the QuantiFast SYBR-Green RT-PCR kit (Qiagen). Standard curves were generated using 10-fold serial dilutions (104 to 108 copies ...
-
bioRxiv - Neuroscience 2022Quote: ... In situ hybridization for mature miR-18a was performed using a miRCURY LNA detection probe (Exiqon/Qiagen, Germantown, MD), labeled with DIG at the 5′ and 3′ends ...
-
bioRxiv - Genetics 2019Quote: ... One-Step RT-PCR reagent (Qiagen # 210212) was used to create cDNA and then the cDNA was amplified by PCR ...
-
bioRxiv - Molecular Biology 2020Quote: ... miRCURY LNA Power Inhibitor against mouse miR-375 (mmu-miR-375-3p) (Qiagen, Hilden ...
-
bioRxiv - Cell Biology 2021Quote: ... 100nM of miR-24-3p inhibitor (Anti-mmu-miR-24-3p, Qiagen, MIN0000219), 100nM of scrambled control (AllStars Negative Control siRNA Print ...
-
bioRxiv - Microbiology 2020Quote: The uniplex RT-qPCR assays to detect DENV were performed with the QuantiTect Probe RT-PCR Kit (Cat. 204445, Qiagen, Hilden, Germany), and the amplification of RT-PCR was performed with Superscript III Platinum One-Step reagents RT-qPCR System (Cat ...
-
bioRxiv - Molecular Biology 2021Quote: ... 40 ng of each sample was used per reverse transcription-quantitative polymerase chain reaction (RT-qPCR) reaction using the QuantiTect SYBR® Green RT-PCR Kit (Qiagen, 204243) on Rotor-Gene Q (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: cDNA from Smad2 null and overexpressing cells and their relative controls was tested using the real-time RT2 Profiler PCR Array (QIAGEN, PAMM-099Z ...
-
bioRxiv - Biophysics 2022Quote: ... Purified protein was diluted in PBS buffer (pH = 7.2) and the fluorescence intensity was recorded at 60 °C in the Rotor-Gene 6600 real-time PCR cycler (Qiagen) for 18 h ...
-
bioRxiv - Microbiology 2021Quote: ... 80 ng of total RNA was added as template for real-time PCR using QuantiFast SYBR green Master Mix (Qiagen) in the StepOne plus instrument (Applied Biosystems) ...
-
Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: ... Thermal cycling was performed on the Rotor-Gene Q real-time PCR cycler and data were analyzed with the associated software (Qiagen) using a cycle threshold of 0.03 ...
-
bioRxiv - Cell Biology 2020Quote: ... The real-time PCR reaction was performed with a Rotor-Gene SyBR Green on a Rotor-Gene Q cycler (Qiagen). Gene expression was calculated as copies/μL using the standard curve approach ...
-
bioRxiv - Immunology 2020Quote: ... The cDNA thus obtained was subjected to quantitative real-time PCR analysis with RT2 SYBR® Green qPCR Mastermix (Qiagen) in 20 µl reaction volumes on a LightCycler 480 thermal cycler (Roche).
-
bioRxiv - Physiology 2020Quote: ... The cDNA was used on the real-time RT2 Profiler PCR Arrays (QIAGEN, PAHS-065Y, PAHS-022Z and PAHS-162Z) in combination with RT2 SYBR® Green qPCR Mastermix (Qiagen) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR (QPCR) using gene-specific primers (supplementary table S9) was performed using QuantiNova SYBR Green mix (Qiagen) on an MX3000P real-time PCR machine (Stratagene) ...
-
bioRxiv - Cell Biology 2019Quote: ... The copy number of standard DNA molecules was then determined by the following formula (from the script “Critical Factors for Successful Real Time PCR”, Qiagen):
-
bioRxiv - Developmental Biology 2021Quote: ... The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen) with specific primers listed in Supplementary Table 1 and a LightCycler 480 (Roche) ...
-
Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... 45 cycles at 95° for 15 sec and 60° for 1 min were performed in the Rotor-Gene Q real-time PCR cycler (Qiagen). The numbers of copies of the RdRP gene in the samples were calculated using an RdRP cDNA standard curve.
-
bioRxiv - Molecular Biology 2023Quote: All tests were run in triplicate and on a Rotor-Gene Q 2plex real time PCR Platform from QIAGEN (Germany).
-
bioRxiv - Molecular Biology 2024Quote: ... we synthesised cDNA using 2 µg of total RNA and cDNA was amplified with a PerfeCTa qPCR fluorescent SYBR Green SuperMix (Quantabio) using real-time quantitative PCR (Rotor-Gene RG 6000— QIAGEN). The primer sequences used for the gene expression are shown in Table S1 ...
-
bioRxiv - Bioengineering 2020Quote: ... and linearly amplified with RT2 miR First Strand Kit (Qiagen, Hilden, Germany), according to manufacturer’s instructions ...