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Citations for Qiagen :
601 - 650 of 10000+ citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Routine qPCR for miR detection and validation (see Supplementary Table 1 for miR sequences) was performed with the miScript PCR control set (catalog number 218380; Qiagen, Germany). The miScript™ miRNA PCR Array Human Serum & Plasma 384HC (Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were normalized for the relative expression of the housekeeping miR103a-3p (hsa-miR-103a-3p, LNA™ PCR primer set, Qiagen), which showed no variability between analyzed groups.
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR reactions were run using a QuantiTect SYBR Green RT-qPCR kit (Qiagen, 204243) on a Qiagen Rotorgene Q ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR reactions were run using the QuantiTect SYBR Green RT-qPCR kit (Qiagen, 204243) on a Qiagen Rotor-Gene Q ...
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bioRxiv - Cancer Biology 2023Quote: ... was reverse transcribed into cDNA by miScript RT II Kit using miScript HiSpec Buffer or miRCURY LNA RT Kit (Qiagen). Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... Amplification was performed using a Rotor-Gene Q real-time fluorescence detector thermocycler (Qiagen) programmed as follows ...
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bioRxiv - Microbiology 2024Quote: The multiplex real-time qPCR assays were carried out in Rotor-Gene Q (Qiagen). A primer mix (200 µl ...
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bioRxiv - Genetics 2024Quote: ... and fluorescence detection were done using the QIAcuity digital PCR instrument (Qiagen). For each assay ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with Omniscript RT Kit (Qiagen). The relative mRNA expression level of each target gene was quantified by qPCR using KAPA SYBR fast qPCR master mix (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Microbiology 2020Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Microbiology 2021Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Cell Biology 2021Quote: ... or miRscript RT kit II (Qiagen, Ref. 218161), respectively ...
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bioRxiv - Immunology 2021Quote: ... reverse transcribed into cDNA (QuantiTect RT kit, Qiagen) and analyzed by RT-qPCR.
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bioRxiv - Immunology 2023Quote: ... and Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using Sensiscript RT Kit (QIAGEN) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... and the miRCURY LNA RT Kit (Qiagen, UK) was used to conduct qPCR in the Roche ® LightCycler® 480 according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system under these cycling conditions ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR experiments were performed with Rotor-Gene-Q machine (Qiagen) using the 1x Takyon SYBR Master Mix (Eurogentec ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative RT-PCR was performed in triplicate using SYBR green mix (Qiagen) on an ABI 7500 machine (Applied Bio Systems) ...
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bioRxiv - Plant Biology 2023Quote: ... RT PCR was carried out in a Rotor-gene Q2 PLEX (QIAGEN), using SensiMix SYBR master mix (Bioline) ...
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bioRxiv - Microbiology 2020Quote: ... These primers were used to amplify the mRNA from the sample using Quantifast SyBR Green one –step RT-PCR kit (Qiagen, Netherlands) and the reactions were run using ABI 7500 real-time PCR instrument (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed in an Applied Biosystems QuantStudio 3 thermocycler using the QuantiNova Probe SYBR Green PCR Kit (Qiagen, Germany); mix proportions and cycling parameters were used as described in manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Specific pre-mRNAs from total RNA samples were initially amplified by cDNA synthesis (14 cycles) using primers overlapping an intron-exon junction and a OneStep RT-PCR kit (Qiagen, #210212). Each reaction used 250 ng of RNA per reaction ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng RNA were reverse transcribed using the QIAGEN OneStep RT-PCR Kit according to the manufacturer’s information (Qiagen, Hilden, Germany). The sequences of primers were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... while URA3 and ACT1 levels were measured by one-step RT-PCR with specific primers (Supplementary Table S3) following the standard protocol of the RNeasy® Mini kit (Qiagen). All strains were propagated for at least 100 generations before analysis.
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reaction was performed on yield cDNA synthetized from each sample using QuantiTec® Rotor-GeneTM SYBR Green RT-PCR kit (Qiagen) using the Qiagility instrument software (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... RT-PCR products were gel purified in 3% agarose gel and cleaned up using QIAGen gel extraction kit (QiaGen, Hilden, Germany).
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-qPCR were performed using primers previously described by Dias de Melo et al [15] using QuantiNova SYBR® Green RT-PCR Kit (Qiagen) according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... RT-PCR for PI3K mRNA was performed using the QuantiTect® Probe RT PCR Kit and the double-stranded DNA stain SYBR green according to the manufacturer’s instructions (Qiagen, ThermoFisher). B-actin was used as the control ...
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA with its genomic DNA ablated was converted into cDNA as instructed by the QIAGEN OneStep RT-PCR Kit (Qiagen, UK). Twenty ng cDNA (relative to RNA amount ...
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bioRxiv - Cancer Biology 2023Quote: RT PCR: Total RNA was eluted from knockdown and control cells using the RNeasy® Plus mini kit (Qiagen, Ref # 74134). 2 µg of total RNA was reverse transcribed using High Capacity cDNA Reverse Transcription Kit (AppliedBiosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Hsa-miR-124-3p Locked Nucleic Acid (LNA) power inhibitor and has-miR-124- 3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) were resuspended with RNase-free water to 100 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR was performed using QIAGEN’s RT2 Profiler™ PCR Array Human Cell Death PathwayFinder (QIAGEN, Hilden, Germany). RT-PCR data was analyzed and visualized for pathway analysis by GeneSpring GX 14.9 (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... Synthetic cel-miR-39 mimic (miScript miRNA Mimics, QIAGEN) was spiked to each RNA sample at a ratio of 11 fmol per ug of total RNA and used as an exogenous control for normalization ...
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bioRxiv - Cancer Biology 2020Quote: ... Ct values for hsa-miR-145-5p (Qiagen, YP00204483) were normalized to expression levels of hsa-miR-423-5p (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Syn-cel-miR-39 spike in synthetic RNA (Qiagen) was added to monitor extraction efficiency ...
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bioRxiv - Cell Biology 2020Quote: ... and a Rotor-Gene Q Rea-time PCR cycler (Qiagen). Data was analyzed using the comparative CT method (Livak and Schmittgen ...
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bioRxiv - Cancer Biology 2020Quote: ... at a final concentration of 50 nM and confirmed by RT-qPCR with the miRCURY LNA™ Universal RT microRNA PCR system (Qiagen) and a StepOnePlus thermocycler (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... and quantitative RT-PCR was performed using SYBR-based primers (Supplemental Table 1) and RT SYBR Green qPCR Mastermix (Qiagen, #330509) on a CFX96 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... 200 ng of RNA was used in the RT reaction with the miScript RT kit (Qiagen). qPCR was performed using a custom LNA probe for miR-1002 using the miRCury SYBR green reagent (Qiagen).
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bioRxiv - Neuroscience 2023Quote: The RT reaction was performed using the QIAGEN miRCURY LNA RT Kit (Cat. No.: 339340, QIAGEN). The reaction mix included 2 µl of 5x miRCURY SYBR® Green RT Reaction Buffer ...
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bioRxiv - Microbiology 2019Quote: ... Purified PCR products (PCR purification kit, Qiagen) of CHPV and JEV positive samples were sent for sequencing ...
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bioRxiv - Bioengineering 2020Quote: ... PCR amplification (Qiagen, Taq PCR Core Kit) was used to create more copies with a T7 promoter that was added to the 5’ end of the forward primer ...
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bioRxiv - Cell Biology 2023Quote: ... PCR purified (QIAquick PCR Purification kit, QIAGEN), and used in T7 reverse transcription reactions (MEGAscript ...
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bioRxiv - Microbiology 2023Quote: ... PCR purification (Qiagen QIAquick PCR Purification Kit) was used to isolate the linearized PCR product ...
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bioRxiv - Microbiology 2020Quote: Real time qPCR (SYBR®Green Applied Biosystems™, CA, USA; RotorGene, Qiagen, Hilden, Germany) was performed on microbial DNA from day 0 (pre-treatment) ...
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bioRxiv - Microbiology 2022Quote: ... Correct introduction of the NS1 mutations was verified via cDNA synthesis of viral RNA using the One-Step RT-PCR kit (Qiagen, Hilden, Germany), amplification and sequencing using NS-specific primers ...
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bioRxiv - Immunology 2020Quote: RNA from B1a B cells was reverse transcribed and amplified using a Qiagen OneStep RT-PCR kit (Qiagen Inc., Valencia, CA, USA) and iRepertoire® mouse BCR heavy chain (MBHI ...
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bioRxiv - Microbiology 2020Quote: ... an aliquot of the extracted RNA solution was added to the reaction mixture for the QuantiTect probe RT-PCR kit (Qiagen, Hilden, Germany), which contained 2x QuantiTect probe RT-PCR master mix ...