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Citations for Qiagen :
401 - 450 of 5763 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... and DNase Set (QIAGEN). Gli1 and Hprt levels were determined by one-step quantitative reverse transcriptase PCR (qRT–PCR ...
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bioRxiv - Bioengineering 2019Quote: Genes were quantified by one-step quantitative reverse transcription-polymerase chain reaction (q-PCR) using the QuantiFast SYBR Green RT-PCR kit (Qiagen, UK). A master mix was prepared by mixing 6.25 μl SYBR green ...
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bioRxiv - Physiology 2023Quote: ... Gene expression of 96 fibrosis-associated genes were evaluated using a PCR array kit (RT² Profiler™ PCR Array Rabbit Fibrosis PANZ-120Z, Qiagen) by quantitative real-time PCR using a Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... with all primers listed in Supplementary Table 1 and then purified (QIAGEN, PCR purification kit). Before nick translation ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative real time PCR was performed using SYBR Green and the following primers from Qiagen: Ppia (QT00247709) ...
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bioRxiv - Immunology 2020Quote: ... 0.125 μl 20 μM SmarterR reverse primer (30) and 1 μl PCR-grade H2O (Qiagen). The amplification was performed in a thermal cycler (lid temperature 98°C ...
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bioRxiv - Genetics 2019Quote: ... genomic regions were PCR amplified using sequence specific primers with HotStarTaq Polymerase Kit (Qiagen # 203203). PCR primer sequences used to amplify the CASK_SS variant ...
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bioRxiv - Cancer Biology 2021Quote: ... we pre-coated 384-well plates with a mixture containing the PCR primer mix (Qiagen; sequences and category numbers available through online data repository (17)) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR primers were designed using the PyroMark Assay Design 2.0 software (Qiagen, Supplementary Table 2). The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed with 9ng of cDNA per reaction using Quantitect primers (Qiagen).
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bioRxiv - Immunology 2024Quote: ... using primers (Supplementary Table 2) and purified the products using QIAquick PCR Purification Kit (Qiagen). PCR-products were Sanger sequenced and knockout scores were determined using the ICE analysis tool (https://ice ...
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bioRxiv - Genetics 2021Quote: ... PCRs were set-up in 30 μl reactions using three μl of 10x HotStarTaq buffer (Qiagen, Hilden Germany), 2.4 μl of 10 mM d’NTPs (Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Sets of 95 ligations were pooled into one sample and purified using QIAquick PCR Purification Kit (Qiagen, Germany). The pooled ligation mixtures (5 μL ...
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bioRxiv - Immunology 2021Quote: ... Primers were from QuantiTect Primer Assays (Qiagen). Expression was normalized to mouse or human Gapdh.
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bioRxiv - Microbiology 2022Quote: ... Primers were from QuantiTect Primer Assays (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... miR-495 pLNA (miRCURY LNA miRNA Power Inhibitor HSA-MIR-495-3P, Qiagen #339130 YI04101229-DCA).
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bioRxiv - Immunology 2023Quote: ... 25 nM and 50 nM of synthetic hsa-miR-30b or control mimics (50 nM) (Qiagen). At 36 h post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward: 5’-GGCACCGAGAAAGACAAAGT-3’; reverse: 5’-CTCTGGAAATGGGCTTCAAA-3’) and PCR products were purified using QIAquick PCR purification kit (QIAGEN, Cat #28104) before Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... We obtained single amplified products for12 out of the 14 PCR primer pairs and these amplified products were purified using the QIAquick PCR Purification Kit (28104, Qiagen, Germantown, MD) before sequencing ...
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bioRxiv - Biochemistry 2020Quote: Reverse transcription reactions containing 2μl of purified RNA and 2μl of 10mM HNqPCRrev1 primer in a final volume of 20μl were performed using the Qiagen Sensiscript RT kit (Qiagen, catalog # 205211) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... All RNAs for the miRNA investigation were transcribed using miScript RT kits and subsequent qPCR performed using miScript Primer Assays and miScript SYBR kits (Qiagen, Courtaboeuf). Experiments were performed with at least triplicates for each data point ...
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bioRxiv - Cancer Biology 2021Quote: RT-PCR was performed using the SYBR green master mix (RealQ plus 2X, Qiagen-Germany). Amplification was done by Exicycler96 (Bioneer-S ...
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bioRxiv - Microbiology 2020Quote: ... vIL-6 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using QuantiTect Multiplex RT-PCR Kits (Qiagen) as described previously (37 ...
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bioRxiv - Cancer Biology 2021Quote: ... and selected genes were amplified by Q-RT-PCR with SYBR green (Qiagen, Valencia, CA) on the FAST Model 7900HT (Applied Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand synthesis and RT-PCR were performed using QuantiTect™ Reverse Transcription Kit (Qiagen) and Rotor-Gene™ SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... EGR1 transcripts were converted into cDNA and quantified using a OneStep RT-PCR kit (Qiagen) and a pair of primers (Table S1) ...
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bioRxiv - Physiology 2020Quote: ... cDNA was prepared by reverse transcription using the Quantitect RT-PCR kit (205313, Qiagen, DEU). cDNAs were amplified using assay-on-demand kits (Gpt ...
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bioRxiv - Cell Biology 2020Quote: ... For the RT² Profiler™ PCR Array Human Epithelial to Mesenchymal Transition kit (EMT) (Qiagen), RNA integrity of all the samples was tested by using the Agilent RNA 6000 Nano kit (Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The harvested contents were subjected to RT-PCR using OneStep Kit (210212, Qiagen, Hilden, Germany). Forward (F ...
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bioRxiv - Physiology 2023Quote: ... Quantitative real-time RT-PCR was performed in a Rotor-Gene Q (Qiagen, Hilden, Germany) using the 5x QPCR Mix EvaGreen (No Rox ...
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bioRxiv - Physiology 2021Quote: ... reaction tubes were transferred to a Rotor-Gene Q PCR thermal cycler for product amplification using a one-step protocol (QuantiFast SYBR® Green RT-PCR Kit, Qiagen, UK). The amplification protocol was as follows ...
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bioRxiv - Neuroscience 2020Quote: ... Those standards were generated by mixing commercially available unmethylated and hypermethylated DNA standards (EpiTect PCR Control DNA Set, Qiagen) resulting in 10 ng of total bisulfite-converted DNA ...
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bioRxiv - Immunology 2019Quote: ... RNA collected for real time PCR experiments was further purified using column DNA digestion (RNase-Free DNase set, Qiagen). cDNA was prepared using iScript cDNA-synthesis kit (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... the reactions were pooled in sets of four and purified on one column each (MinElute PCR purification kit, Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... using miScript Primer Assays for miRNAs and the using the QuantiTect SYBR Green PCR kit (Qiagen) for mRNA in a Stratagene Mx3005P qPCR machine (Agilent) ...
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bioRxiv - Genetics 2020Quote: ... Primer design was optimised to work with the Type-it Microsatellite PCR kit (QIAGEN, Hilden, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... with primers pBEST_LinL_F and pBEST_LinL_R (Table S1) and subsequent purification using QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... and pJET_Luc_FL_UTR_REV (5’-GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAAATGC-3’) primers and was purified with the QIAquick PCR Purification Kit (Qiagen) directly after the PCR reaction ...
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bioRxiv - Immunology 2020Quote: Primer pairs were used in this study together with a QuantiFast SYBR green PCR kit (QIAGEN). The sequences of primer pairs used in this study are listed in Supplementary Table 3 ...
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bioRxiv - Immunology 2023Quote: ... and the purified pools were barcoded with Illumina indexing primers using a Multiplex PCR Kit (QIAGEN). All indexed pools for each sample were then pooled during column purification ...
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bioRxiv - Physiology 2019Quote: ... primers obtained from Qiagen (Quantitect Primer Assay, QT00101962) were used with an expected band length of 96bp ...
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bioRxiv - Cell Biology 2022Quote: The reverse primer was miScript Universal Primer (Qiagen).
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bioRxiv - Molecular Biology 2022Quote: Myotubes were transfected using either 5nM hsa-miR-148a-3p miRCURY LNA miRNA Mimic (QIAGEN, YM00472598-ADB) or negative control miRCURY LNA miRNA Mimic (QIAGEN ...
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bioRxiv - Plant Biology 2022Quote: ... RNase-Free DNase Set (Qiagen) was used to remove DNA ...
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bioRxiv - Immunology 2022Quote: ... RNase-free DNase Set (Qiagen) was used to remove potential DNA contamination ...
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bioRxiv - Genomics 2019Quote: ... and DNase set (79254; Qiagen), followed by delusion with 10 mL of RNase-free water ...
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bioRxiv - Neuroscience 2021Quote: ... dNTP set was from Qiagen. Plasmid purification kits were from ROBOKLON.
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bioRxiv - Plant Biology 2021Quote: ... RNase-Free DNase Set (Qiagen) was used according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... RNase-Free DNase Set (QIAGEN) was used to remove all genomic DNA ...
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bioRxiv - Cell Biology 2024Quote: ... RNase-Free DNase Set (Qiagen) was used to remove genomic DNA contaminants ...