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Citations for Qiagen :
351 - 400 of 5763 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Expression of miR-181a was validated by q-PCR using primers (cat # MS00008827, Qiagen).
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bioRxiv - Physiology 2020Quote: ... or the QuantiTect PCR Kit with self-designed primers for neuronal factors (Qiagen, Germany) (Supplementary Table 3) ...
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bioRxiv - Microbiology 2020Quote: Bact27F and Bact1492R primers either by directly sequencing agarose-gel purified PCR products (Qiagen), or TA cloning (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative real time-PCR was performed with specific primers from QIAGEN (Valencia, CA, USA) and detection reagent SYBR Green mix (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene-specific primers were used for end-point PCR (HotStarTaq Plus DNA Polymerase; Qiagen), to detect inclusion or exclusion of alternative exons ...
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bioRxiv - Neuroscience 2023Quote: ... The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen). Product amplification was performed using the following reaction mixture ...
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bioRxiv - Microbiology 2020Quote: ... Positive sense vRNA and GAPDH transcripts were measured by qRT-PCR using the QuantiTect SYBR Green RT-PCR kit (Qiagen) on an Applied Biosystems 7300 machine ...
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bioRxiv - Physiology 2020Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Immunology 2019Quote: Total RNA samples were diluted to a concentration of 10 ng/μl prior to gene expression studies by qRT-PCR using QuantiFast SYBR green RT-PCR kit (Qiagen) in 7900HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The remaining 18 μl of RT-PCR DNA was purified by a QIAquick PCR Purification kit (Qiagen, Germantown, MD, USA). Sequences of the purified RT-PCR products were generated using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and viral genome copies quantified by real-time PCR using the OneStep RT-PCR Kit (Qiagen) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... Synthetized cDNA was subjected to a PCR array specific for the human antiviral response (RT² Profiler PCR array – PAHS-122Z, SA Biosciences, Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... we used both one-step RT-PCR and two-step (cDNA synthesis followed by separate PCR amplification) reaction designs with commercial One-Step Ahead RT-PCR Kit (Qiagen) or IEH in-house RT ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl total RNA and QuantiTect Probe RT-PCR Kit (Qiagen). Amplification and analysis were performed using QuantStudio3 system (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The PNPLA2 transcript levels in ARPE19 cells determined by quantitative RT-PCR were normalized using the QuantiTect SYBR Green PCR Kit (Qiagen) in the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed with 100ng of total RNA using the QuantiFast SYBR Green RT-PCR kit (Qiagen, Singapore) detector system (ABI Quant studio 5 ...
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bioRxiv - Cancer Biology 2020Quote: Quantitative real-time RT-PCR (qRT-PCR) amplification was performed on RNA extracted from cells using RNeasy Mini kit (#74104, Qiagen). RNA (50 ng ...
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bioRxiv - Neuroscience 2020Quote: ... The quantitative real-time RT-PCR (qPCR) analysis for Bcl2l1 and Bcl2l11 was performed using a SYBR Green PCR Mix (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative RT-PCR analysis of the samples was carried out using the mouse antiviral response RT2 Profiler PCR array (Qiagen) on a 7900HT Fast Real-Time PCR system (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 75 ng of rRNA was reverse transcribed and amplified with 8 PCR cycles using the OneStep RT-PCR kit (Qiagen) and primers MS2_quant_F and MS2_quant_R (Supplemental Table 5) ...
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bioRxiv - Cancer Biology 2020Quote: ... Digoxigenin labeled miRCURY LNA probes (HSA-MIR-10B-5P, SCRAMBLE-MIR, miRCURY LNA U6, Qiagen) were diluted in hybridiziation buffer after a denaturation step at 90 °C and incubated on cells for 30 min (60 min tissue sections ...
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bioRxiv - Cell Biology 2020Quote: ... and run on the RT^2 First Strand Kit (Stem cell PCR array) (Qiagen). The array was run in triplicate (N=3 ...
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bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Neuroscience 2019Quote: ... All the qPCR experiments were performed using Quantifast SYBR Green RT-PCR Kit (Qiagen) in a LightCycler 480 II System (Roche) ...
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bioRxiv - Immunology 2019Quote: ... Human RAC2 3’UTR was amplified with One Step Ahead RT-PCR kit (Qiagen) from human mRNA using the following primers RAC2 3’UTR+ ...
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bioRxiv - Cancer Biology 2021Quote: ... One-step qRT-PCR reactions were performed in triplicate using QuantiTech RT mix (QIAGEN) on the Bio-Rad C1000 Thermocycler and CFX96 system (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed by using QuantiTect SYBR® Green PCR Kit (#204145, QIAGEN) on Agilent MP3005P thermocycler ...
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bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Microbiology 2019Quote: ... For this purpose we used the QIAGEN OneStep RT-PCR Kit (Qiagen, CA-EUA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Ig Vκ and Vλ from positive wells were amplified by RT-PCR (210210; QIAGEN) and nested PCR (203205 ...
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bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Molecular Biology 2021Quote: ... TER1 was amplified from circularized RNA with the OneStep RT-PCR Kit (Qiagen, 210210) (primer sequences in Table S6) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time RT-PCR was performed on a Rotor-Gene Q instrument (Qiagen) using SYBR Premix Ex-Taq II (TakaraBio) ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 RNA was analyzed using the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a LightCycler 480 (Roche) ...
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bioRxiv - Immunology 2021Quote: ... Viral load was quantified by Taq-Man qPCR using OneStep RT-PCR Kit (Qiagen) and AriaMx Real-time PCR Systems (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... RNA (500 ng) was reverse transcribed into cDNA using QuantiTect RT-PCR kit (Qiagen). RT-PCR was performed on a BioRad CFX384 thermocycler using pre-validated Taqman primers (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen). qPCR was performed using the QuantStudio 7 Flex System ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in triplicate using the QuantiNova SYBR Green PCR Kit (Qiagen) and the following primer sequences ...
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bioRxiv - Physiology 2023Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Each sample was analysed in duplicate ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-qPCR was performed using the miScript SYBR Green PCR Kit (Qiagen, 218073) together with the miRCURY LNA miRNA PCR Assay (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... RT-PCR was normalized using the housekeeping genes hypoxanthine phosphoribosyl-transferase 1 (HPRT1, Qiagen QuantiTect Primer Assay ...
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DNA and RNA-SIP reveal Nitrospira spp. as key drivers of nitrification in groundwater-fed biofiltersbioRxiv - Physiology 2019Quote: ... and from sacrificed column experiments and full scale biofilters were reverse transcribed using reverse primer 1492R with Sensiscript RT kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Three micrograms of RNA were used to synthesize cDNA with the Omniscript RT kit using oligo dT primers (Qiagen 205113). Resulting cDNA was diluted 1:2 and used for real-time PCR with Power SYBR Green (Thermo Fisher Scientific 4367659 ...
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bioRxiv - Molecular Biology 2021Quote: The gene of interest was amplified using PCR with gene-specific primers and purified using the QIAquick PCR Purification Kit (QIAGEN, Germany). Gene-specific T7 promoter sequences were added to the 5’ and 3’ end of the purified product using PCR and were purified ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR was performed using 20 miRNA primers (Supplementary Table 1) and miScript SYBR Green PCR Kit (Qiagen, Hilden, Germany). RNA U6 was used as the endogenous control ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed for all primers in 10μl reaction containing 4μl Qiagen multiplex PCR buffer mix (QIAGEN Inc., Hilden, Germany), 0.3 μM primer mix for each set ...