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Citations for Qiagen :
401 - 450 of 717 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Biotinylated mouse anti-5xHis antibody was purchase from QIAGEN. Mouse anti Tubulin beta 3 antibody from BioRad (Hercules ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... or mouse anti-RGS-His antibody (Qiagen; cat # 34610) at 1:5000 dilution followed by goat anti-rabbit-HRP conjugate (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-penta-His antibody (34660; Lot# 136244018; Qiagen); mouse anti-Myc tag mAb ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Plant Biology 2022Quote: ... and NoFa-ICL was the Penta-His antibody (Qiagen) at 1:4000 in TBST ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).
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bioRxiv - Biophysics 2023Quote: ... The antibodies were anti-RGSHis (Qiagen, Cat. No. 34650) and peroxidase-conjugated anti-mouse antibody (Dako ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was collected and subjected to affinity purification using 5 mL Hi-Trap column containing Ni-NTA resin (Qiagen) on an AKTA Pure 25L protein purification system (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the two assay plasmids by temperature-cycle ligation.
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bioRxiv - Biochemistry 2021Quote: ... and cDNA products containing the R2R adapter attached to their 5′ end were cleaned-up by using a MinElute column (Qiagen) to remove unused primer ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with lysis buffer were pulverized in a 10 mL jar containing a 12 mm grinding ball using a TissueLyser II (QIAGEN) by performing 5 rounds of 3 min at 15 Hz ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Single cells of Desulfovibrio or Methanococcus were sorted into wells of a 96-well plate containing 3 µl of PBS and Buffer D2 from Repli-G single cell kit (Qiagen) by using Influx flow cytometer (BD) ...
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bioRxiv - Developmental Biology 2020Quote: ... and for H2b-GFP the band at 5263bp (containing the H2b-GFP) was excised and DNA was extracted using Qiagen QuickGel extraction kit (28704, Qiagen). Ligations were set up with a molar ratio 3:1 of insert:vector ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from IEC isolations by adding a volume containing 2 x 106 cells in PBS to QIAshredder spin columns (QIAGEN) followed by centrifugation at 9,300 g for 1 min ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21 (DE3) as fusion proteins containing an N-terminal His6- tag and purified by immobilized metal-affinity chromatography (Ni-NTA, Qiagen). Full-length DosR was also expressed with N-terminal GST-tag ...
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bioRxiv - Microbiology 2019Quote: ... The presence of the HCV replicon was determined by sequencing the NS4A-containing region following cDNA synthesis on extracted RNA (RNeasy RNA extraction kit, Qiagen) and PCR amplification of the NS4A region ...
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bioRxiv - Immunology 2019Quote: ... longitudinal muscle/myenteric plexus preparations were homogenized in a 2 ml eppendorf containing 1 ml Trizol and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Immunology 2019Quote: ... mucosal scrapings and feces were homogenized in a 2 ml eppendorf containing 1 ml of DNA extraction buffer and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Genomics 2019Quote: ... We used a 100 µm nozzle to sort single cells into 96-well plates containing 5 µl TCL buffer (Qiagen) with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... 16 equivalent slices were obtained from two roots (replicates) and collected in individual tubes containing RTL buffer (Qiagen MicroRNAeasy Kit). Samples were flash frozen by placing liquid nitrogen in the tubes ...
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bioRxiv - Systems Biology 2020Quote: ... followed by flow sorting with a Sony SH800Z (gating on calcein NVOC fluorescence levels) into individual wells of a 96-well plate containing 5 μL of Buffer RLT (Qiagen) and 1% β-mercaptoethanol.
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bioRxiv - Microbiology 2021Quote: ... For next generation sequencing RNA from virus-containing samples were extracted using the QIAsymphony DSP Virus/Pathogen mini kit (Qiagen). RNA was DNase-treated using the TURBO-free Kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... 10 to 20 mg of frozen tissue was dissociated using 400 μl of RLT Plus in a 2mL extraction tube containing a 5 mm diameter beads (Qiagen) and agitated at 30 HZ for twice 2 minutes in the TissueLyser II (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in either glutamine-containing or glutamine-free medium for 24 h and then used for total RNA isolation using the RNeasy kit (Qiagen). Sample preparation for analysis and processing of the arrays were performed using the protocol provided by Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... frozen tissue was partially thawed and submerged in RLT lysis buffer containing 1% ß-mercaptoethanol and 0.5% reagent DX before being homogenized using a hand-operated TissueRupture (Qiagen). To pool samples in equal proportions ...
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bioRxiv - Microbiology 2020Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA). A minimum of 20 clones from each sample were sequenced ...
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bioRxiv - Microbiology 2021Quote: 8×107 cells from undifferentiated or methylcellulose differentiated keratinocytes containing HPV18 (WT or ΔCTCF) samples for RNA extraction using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions and DNaseI treated (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... frozen tissue was partially thawed and submerged in lysis buffer containing 1% ß-mercaptoethanol and 0.5% Reagent DX before tissues were homogenized together with TissueRupture (Qiagen). The homogenate was centrifuged to remove any potential tissue residues ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... were homogenized in 2-mL tubes containing lysis buffer plus 1% 2-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless-steel beads (Qiagen #69989 ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 5 μl of purified RNA tagged with adapters containing a Unique Molecular Index (UMIs) was converted into cDNA NGS libraries using the QIAseqmiRNA Library Kit (QIAGEN). cDNA was amplified in 22 cycles of PCR and purified ...
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The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... The retrieved tissue mROIs were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
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Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projectionsbioRxiv - Developmental Biology 2022Quote: ... Sorted ßGal+ or GFP+ cells were collected in 1.5ml DNA LoBind® tubes placed at 4°C containing 400µl RLT buffer (Qiagen, 79216) with 1% β-Mercaptoethanol (Millipore Sigma ...
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bioRxiv - Cell Biology 2022Quote: Pellets were thawed and lysed on ice in 15 mL lysis buffer containing freshly added lysozyme and benzonase prepared according to manufacturer instructions (Qiagen Qproteome Bacterial Protein Prep Kit ...
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bioRxiv - Developmental Biology 2022Quote: Whole organoids were washed once with 1X PBS and placed into 2ml microcentrifuge tubes containing one 5mm steel bead (Qiagen) and 1ml of Trizol reagent (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dissected discs were transferred with a P-20 pipette to a 1.5 ml tube containing 350 ul of the RLT buffer (in RNeasy mini kit, Qiagen 74104) with 1% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: The plasmid #TH8-77 was digested by Not I and DNA fragment containing cdc8 gene fused with mNeongreen and a 40 aa linker were purified by gel extraction (Qiagen). Obtained DNA fragment was used for the transformation of MBY101 strain (h- ...
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bioRxiv - Molecular Biology 2022Quote: ... Sample was cleaned using a magnetic rack and the supernatant containing DNA was purified with a MinElute PCR Purification Kit (Qiagen). Libraries were prepared according to manufacturer’s instructions using the following kits ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... per condition or genotype were homogenized in 2-ml Eppendorf tubes containing lysis buffer with 1% beta-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless steel beads (Qiagen #69989) ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were centrifuged 15,000 x g at 4°C for 30 min and the resulting supernatant containing the target protein was filtered and purified using a pre-equilibrated Ni-NTA beads column (Qiagen). The recombinant protein was eluted using 4 mL of elution buffer (8 mM Na2HPO4 ...
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bioRxiv - Neuroscience 2021Quote: ... RNA extraction from 50 μl of nuclei suspension from FACS (containing 5000 sorted NeuN+ nuclei) was conducted using RNeasy Mini Kit (74104, Qiagen) and RNase-Free DNase Set (79254 ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: ... placed in 250 μL PBS containing a 5 mm stainless steel bead and homogenized using a tissue lyser (Tissue-Lyser II, Qiagen) for 2-minutes at 30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... cDNA MERS-D1 (91) containing basepairs 12259–15470 of the MERS-CoV genome was digested with BgII and purified by Qiagen QIAquick PCR Purification Kit to be used as a standard in the RT-PCR reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... For tissue disruption frozen biopsy samples were transferred to ceramic bead-filled tubes (#KT03961-1-102.BK, Bertin Technologies, Montigny-le-Bretonneux, France) containing 700 µl QIAazol Lysis reagent (#79306, Qiagen) and homogenized for 2x 20 sec at 5000 rpm using a Precellys 24 tissue homogenizer (Bertin Technologies).
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from each fecal containing tube using the Tissue and Blood DNA Isolation kit (Qiagen, Valencia, CA) following the manufacturer’s instructions with slight modifications ...