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Citations for Qiagen :
201 - 250 of 753 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... right apical lobes were collected into 2mL tubes containing 0.75mL of Qiazol lysis reagent (Qiagen) and 3mm glass beads ...
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bioRxiv - Genomics 2020Quote: A 560 μL volume of lysis buffer containing guanidinium and carrier-RNA (QIAGEN, Hilden, Germany) was added to 200 μL supernatant from cell cultures ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was loaded onto gravity column containing 5 ml Ni-NTA affinity resin (Qiagen) pre-equilibrated and washed with 30 ml of Buffer A then eluted with Buffer A containing 300mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... 20 μL reactions consisted of 1×QuantiFAST reaction mix containing ROX reference dye (Qiagen, Germany), 0.66 µM of forward and reverse primers ...
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bioRxiv - Cancer Biology 2021Quote: ... we pre-coated 384-well plates with a mixture containing the PCR primer mix (Qiagen; sequences and category numbers available through online data repository (17)) ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Samples were transferred to a fresh tube containing 50 μl of Ni-NTA agarose (QIAGEN) and incubated for 60 min at 4°C on a nutator ...
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bioRxiv - Genomics 2022Quote: ... Plasmids containing the PCR product were extracted with the QIAprep Spin Miniprep Kit (Qiagen, Germany). Plasmid products were Sanger sequenced by Macrogen Europe (Amsterdam ...
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bioRxiv - Immunology 2022Quote: ... single-cells were sorted in 96-well plates containing 5 µL of TCL buffer (Qiagen) with 1% β-mercaptoethanol according to the gating strategy shown in Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant was added to a gravity column containing 4 mL of Ni-NTA (Qiagen). Beads were washed with a high salt buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1 % SDS) containing protease inhibitors and 0.1mM of 1,10- phenanthroline using a TissueLyser II (QIAGEN). The samples were then quantified ...
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bioRxiv - Microbiology 2024Quote: Right apical lobes were collected into 2mL tubes containing 0.75mL of Qiazol lysis reagent (Qiagen) and 3mm glass beads ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant containing PqsE was incubated with 1.5 mL of previously washed Ni-NTA resin (Qiagen) and mixed at 4 °C for 1 h ...
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bioRxiv - Genetics 2024Quote: ... ChIP-DNA was treated with 250 μL 1X TE containing 100 μg RNase A (Qiagen) and 5.0 μL of 20 mg/mL Proteinase K (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... RNA extractions substituted lysis buffer containing proteinase K solution in PKD buffer (1:16) (Qiagen) for the Mag-Bind beads used in the manufacturer protocol.
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bioRxiv - Cell Biology 2023Quote: ... Cells from the inserts were lysed and collected into RLT buffer containing B-mercaptoethnaol (QIAGEN) for downstream molecular analyses by RT-qPCR.
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing the His6-Nsp8 was loaded onto the Ni-NTA agarose column (Qiagen). The nickel column was extensively washed with wash buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants containing soluble proteins were applied to a 5 mL NiNTA-agarose gravity column (Qiagen) pre-equilibrated with lysis buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... and collected directly into RNase-free microfuge tubes containing 400 µL RLT lysis buffer (QIAGEN) supplemented with 4 µL β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing the His6-Nsp15 was loaded onto the Ni-NTA agarose column (Qiagen). The nickel column was extensively washed with wash buffer (25 mM HEPES-NaOH (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified from samples containing more than 500,000 cells using an RNeasy kit (Qiagen) following the protocol without the optional on-column DNase digestion step ...
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bioRxiv - Biophysics 2024Quote: ... and the nanodisc fractions containing mEGFP-fusion pdP2X7 were bound to Ni-NTA (Qiagen, USA) resin preequilibrated with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen) for 2 h at 4 °C using 1 mL of resin per mg GFP ...
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bioRxiv - Plant Biology 2024Quote: ... Leaf samples were collected in the well containing a 3 mm tungsten carbide bead (Qiagen) and 100 µl extraction buffer (200 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were lysed in RLT buffer containing β-mercaptoethanol (RNeasy Micro Kit, Qiagen, Hilden, Germany). RNA was extracted using the same kit ...
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bioRxiv - Microbiology 2019Quote: ... and HRP-penta-His antibody (Qiagen) and HRP-anti-mouse IgG (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... The PentaHis antibody (Qiagen, Düsseldorf, DE) against His-Tagged proteins was diluted 1:5000 in TBS buffer containing 3% BSA and the membrane was incubated overnight at 4°C followed by 2×10 min washing steps in TBST and 1×10 min in TBS buffers ...
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bioRxiv - Biophysics 2023Quote: ... anti-Strep-tag antibody (Qiagen, 34850), or anti-HA-tag antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... A mouse anti-GST antibody (Qiagen) diluted (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Cell Biology 2019Quote: ... 20ng of total DNA was used in a qPCR reaction containing QuantiNova SYBR Green mix (Qiagen), which was ran on a StepOnePlus Real-Time platform (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... The clarified supernatant containing the target protein was incubated with pre-equilibrated Ni-NTA resin (Qiagen) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid containing the cloned chitinase gene was isolated using the Qiaprep spin miniprep kit (Qiagen). Restriction digestion of the plasmid was done with XhoI and XmaI ...
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bioRxiv - Molecular Biology 2021Quote: ... the upper aqueous phase containing RNA was automatically purified with an RNeasy Plus Mini Kit (QIAGEN) on a QIAcube instrument (QIAGEN) ...
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bioRxiv - Neuroscience 2019Quote: All plasmids containing reporter transgenes were prepared using Qiagen Plasmid Mini Kit (Qiagen Ref No.12125). The plasmids were sent to Genetivision Corporation for injection ...
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bioRxiv - Genetics 2019Quote: ... for lysis in 2 ml safe-lock tubes containing one 5 mm stainless steel bead (Qiagen) for 2.5-3 minutes at 30 Hz using TissueLyzer II (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: Whole blood (10 μL) was collected in an RNase free Eppendorf tube containing lysis buffer (QIAGEN) and stored at −80°C until use ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing hexahistidine-tagged proteins was passed over a column of Ni2+-NTA matrix (Qiagen) at 0.5 mL matrix / 1 L of culture ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted from regions containing mainly malignant cells using Allprep RNA/DNA kit from Qiagen.
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bioRxiv - Physiology 2021Quote: ... The upper phase containing RNA was further processed using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and proteinase K solution (20 mg/mL, Qiagen), followed by an incubation with vortex and lysis steps described in the kit ...
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bioRxiv - Cell Biology 2021Quote: The clarified lysate was then loaded onto an open column containing pre-equilibrated Ni-NTA (Qiagen). The column was washed with 20 mM Tris-HCl ...
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bioRxiv - Pathology 2022Quote: Total DNA containing nuclear DNA and mtDNA were isolated from LB using the DNeasy Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and 2 mg/mL of proteinase K (Qiagen), and incubated at RT for 10 min ...
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bioRxiv - Genomics 2022Quote: ... the supernatant containing the DNA was purified with Genomic-tip 100/G (Qiagen; Cat. no. 10243) with the Genomic DNA Buffer Set (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... DNA containing an internal etheno-dA modification was purified using a QIAquick PCR Purification Kit (Qiagen), processed by translesion DNA synthesis using a mixture of Sulfolobus DNA Polymerase IV and Vent (exo- ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen tissues were placed into tubes containing a 5 mm stainless steel bead (Qiagen, Courtaboeuf, France) and 1 ml of Trizol reagent (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... Large quantities of plasmids containing verified inserts were obtained using the plasmid Maxi kit from Qiagen. The plasmids were linearized using NotI (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen) in Bionano Lysis Buffer according to the Bionano Prep Cell Culture DNA Isolation Protocol ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was extracted from the HD MSN-containing cultures using the RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality was quantified on the NanoDrop Spectrometer and 0.5 to 2 μg of RNA was synthesised to cDNA using the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... 2 mL cultures were immediately added to centrifuge tubes containing 4 mL RNAprotect Bacteria Reagent (Qiagen), vortexed for 5 seconds and incubated at room temperature for 5 min ...