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Citations for Qiagen :
401 - 450 of 526 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Quantitative RT-PCR was performed using 30 ng of total RNA and QuantiTecT sYBR Green RT-PCR kit (Qiagen, 204243) according to the supplier’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs were purified from 3 mL overnight cultures grew at optimum temperature (30 °C or 37 °C) by Dneasy Blood & Tissue Kits (QIAGEN). Additionally ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysate was cleared by centrifugation (20L000 rpm, 30 minutes, 4 °C) and purified using Ni+2-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns (flow 5 mL/min ...
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bioRxiv - Biochemistry 2023Quote: ... The enzyme was inactivated at 75°C for 30 min and genomic DNA was purified using the QIAamp DNA Micro Kit (Qiagen). HLA class I (HLA-A ...
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 37°C overnight in presence of a selective antibiotic Chloramphenicol (30 µg/ml) and isolated the final plasmid using a QIAprep Spin Miniprep kit (Qiagen) the following day ...
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bioRxiv - Biophysics 2023Quote: ... and precipitated with ammonium sulfate as in Niu et al.49.The precipitate was dissolved in 30 ml buffer C and loaded onto a 5 ml column of Ni-NTA-agarose (Qiagen) pre-equilibrated in buffer C ...
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bioRxiv - Molecular Biology 2023Quote: The libraries were purified with a 30-minute incubation at 37 °C in the presence of 160 μg/mL RNase A (Qiagen), a treatment with 200 μg/mL Proteinase K (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... Arabidopsis thaliana frozen leaf tissues were weighed and ground by using two 3 mm stainless steel beads for 3 minutes at 30 Hz with frozen adapters on a TissueLyser II (Qiagen). The resulting frozen powder was dissolved in 650 µL chloroform-methanol (3:7 ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
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bioRxiv - Genomics 2023Quote: ... Swabs were incubated at 37 degrees Celsius for 30 minutes then bead beaten for 6 minutes at 30Hz in a TissueLyser II (Qiagen). The rest of the protocol was followed increasing the volumes to account for the additional lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Following complete removal and evaporation of residual ethanol (10 mins at 30°C) DNA was extracted using the AllPrep DNA/RNA FFPE Kit (Qiagen). DNA was eluted in 40 µl Elution buffer.
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bioRxiv - Microbiology 2023Quote: ... Template DNA was degraded using RQ1 DNAse for 30 min at 4°C and RNA was purified using RNAeasy mini kit (Qiagen). 4-week-old NRG mice were injected intra-hepatically using 10 µg of RNA in a maximum volume of 50 µL of PBS+RNA and one week post injection a terminal bleed was performed ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Microbiology 2023Quote: ... an unbiased amplification method was employed by amplifying the purified DNA at 30°C for 8 hours using a REPLI-g Single Cell kit (Qiagen). The amplified DNA was purified using AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from Library-Diverse was isolated by Blood & Cell Culture DNA Maxi Kit (Qiagen; 30 million cells per condition). Genomic DNA from the TRIP library editing experiments was isolated by DNeasy Blood & Tissue Kit (4 million cells per condition) ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting DNA pellets were washed with 1.0 mL 70% ice-cold ethanol and re-suspended in 30 µL of EB buffer (Qiagen, Germany) after drying.
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bioRxiv - Microbiology 2023Quote: Up to 30 mg of tissue was homogenized in RLT buffer and RNA was extracted using the RNeasy kit (Qiagen), whereas RNA was extracted from bronchoalveolar lavage fluid and swabs using the QiaAmp Viral RNA kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Approximately 50 mg of tissue was homogenised in 500 µl phosphate-buffered saline (PBS) for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen). Total nucleic acids were extracted using the Nucleo Mag Vet Kit (Macherey & Nagel ...
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bioRxiv - Biochemistry 2023Quote: ... shaking overnight at 30°C for 16hrs and the plasmid DNA is purified using a QiaPrep 96 column DNA purification kit (Qiagen). This procedure was conducted twice on different days to produce two separate replicates.
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bioRxiv - Bioengineering 2023Quote: ... Lysate supplemented with 20 mM imidazole was cleared by centrifugation at 15000 g at 4 °C for 30 minutes and the supernatant was then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Microbiology 2023Quote: ... preceded by a 10-minute bead-beating step at 30 Hz in 2 ml e-matrix tubes (MP Biomedical, USA) using a Tissuelyser II (Qiagen). Molarity and fragment-length distribution of the extracts were measured using a Tapestation ...
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bioRxiv - Cancer Biology 2024Quote: ... Final samples of 3 or 30 million cells were collected and genomic DNA was extracted (DNeasy blood and Tissue kit, Qiagen). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... on a Retsch MM400 mixer mill for 60 seconds at 30 Hz and DNA was extracted using the DNeasy Plant Mini kit (Qiagen). DNA was eluted in 100 µL of elution buffer AE (Qiagen) ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysates were centrifuged at 10000 g for 30 min at 4 °C and the clear supernatant was collected and passed through Ni-NTA agarose column (Qiagen). Contaminating proteins were washed away by passing a 20–120 mM imidazole gradient through the column while the bound toxins were eluted using PBS containing 500 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: RNA was purified from 30 μL of anti-FLAG IP eluates using a RNeasy Micro RNA extraction Kit (Qiagen, 74004) with on column DNaseI treatment and the RNA was eluted using 14 μL of DNA-free distilled deionized H2O ...
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bioRxiv - Molecular Biology 2024Quote: ... Expanded clones had mCherry expression measured by flow cytometry (LSRII) 30-46 dpn and DNA isolated on the columns (DNA Blood and Tissue Kit, Qiagen).
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bioRxiv - Immunology 2024Quote: ... was added to each tube and homogenized at 30 rev/s for 3 minutes for 2 rounds using a tissue homogenizer/lyser (#9003240, Qiagen). Kidney extracts were centrifuged at 17,000 RCF for 10 minutes and the supernatant was transferred to a 1.5 mL microcentrifuge tube and kept over ice for 1-2h ...
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bioRxiv - Genomics 2024Quote: ... was performed for 30 min at 37 °C and the resulting fragments are purified using MinElute PCR Purification Kit (Qiagen). qPCR was performed to determine the optimal number of cycles necessary for each library ...
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bioRxiv - Cell Biology 2024Quote: Lung tissues (up to 30 mg) were homogenized in RLT buffer and RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were further homogenized by adding a pre-frozen stainless steel bead to each tube and high-speed shaking (30 seconds, 25 rpm) using TissueLyser II (QIAGEN). For secondary metabolites extraction ...
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bioRxiv - Microbiology 2024Quote: ... all samples were vortexed 15-30 seconds and 500 µL of the eNAT buffer was used for automatic extraction using PowerSoil Pro Ht kit (QIAGEN). Negative extraction controls were included at regular time points throughout the study ...
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bioRxiv - Neuroscience 2024Quote: ... was extracted and purified from pools of 30 zebrafish larvae at 6 dpf using the RNeasy Plus Mini Kit (Qiagen, Germantown ...
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bioRxiv - Microbiology 2024Quote: ... were used to swab the footpad skin extensively for at least 30 sec and collected in PowerBead Pro Tubes (Qiagen). DNA was isolated using the DNeasy PowerSoil Pro kit (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... 30 μL proteinase K and 500 μL buffer AL buffer are added from the DNAeasy Blood & Tissue Kit (Qiagen #69504). Samples are incubated at 56°C for 12-16 hr ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was isolated from a volume of lung homogenate equivalent to 30 mg of tissue using the RNeasy Tissue Kit and RNase-Free DNase Set (both Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... After 24 hours of culture (around 30% confluency), cells were transfected with siCTR (Cat# 51-01-14-03, Integrated DNA Technologies) or siS1PR1 (Cat# SI00376201, Qiagen) using Lipofectamine RNAiMax Transfection Reagent (Cat# 13778150 ...
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bioRxiv - Developmental Biology 2024Quote: ... meioc cDNA encoding 356 amino acid residues from N-terminus and ythdc2 cDNA encoding amino acid residue Arg743 to Leu1381 were cloned into a pQE-30 vector (QIAGEN) and a pET-21a (+ ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was clarified with ultracentrifugation at 30,000 x g for 30 min and loaded onto Ni-NTA resin (Qiagen; 30210). After two washes in the Lysis Buffer and one in the Lysis Buffer with 40 mM imidazole ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and transcripts detected in a one-step RT–qPCR reaction using 30 ng of total RNA and Quantitect SYBR green reagent (Qiagen). Data were normalized against the control gene RPLPO or TUBB5 ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from approximately 30 mg of brain tissue using the RNeasy mini kit (Qiagen, Toronto, ON, Canada) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... whereas 30 mg of lung tissue was homogenized in 600 μl RLT-buffer containing β–mercaptoethanol for 30 sec at 15 Hz using the TissueLyser II (Qiagen).
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bioRxiv - Evolutionary Biology 2021Quote: ... The reaction was incubated at 37°C for 30 min and purified using a MinElute Reaction Cleanup Kit (Qiagen, Germantown, MD). The PCR reaction consisted of 30 μl transposed DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 2000 ng of plasmid DNA was transfected into each well using Effectene (16 μL of enhancer and 30 μL of Effectene reagent; Qiagen 301427). As above ...
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bioRxiv - Immunology 2021Quote: ... 30mg of whole spleen RNA was extract via 30 second homogenization in RLT lysis buffer containing 1% β-mercaptoethanol per RNeasy kit (Qiagen). RNA was reverse transcribed into cDNA using iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Genomics 2021Quote: ... The transposition reaction was incubated at 370C for 30 min and followed immediately by purification using QIAGEN MinElute Kit (Qiagen, 28204). Following steps were primarily based on the protocol described by Buenrostro et al. ...
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bioRxiv - Cell Biology 2021Quote: ... treated with DNase I at 37°C for 30 min and finally cleaned with the miniElute kit (QIAGEN, Catalog Number: &28004). For next-generation sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... by shaking with two 3-mm glass beads for 2 min at 30 Hz with a Tissue-Lyser (Qiagen, Hilden, Germany). After 5 min in an ice-cold ultrasonic bath ...
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bioRxiv - Genomics 2021Quote: ... The resultant nuclei were then incubated for 30 min at 37°C with an in-house made Tn5 enzyme and purified with the Qiagen MinElute kit (Qiagen, 28004). PCR reactions for each replicate were performed with 15 cycles using Ad1F and Ad2.2R primers and NEBNext High-Fidelity 2X PCR Master Mix (New England Labs Cat #M0541) ...
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bioRxiv - Plant Biology 2021Quote: ... The dissected cells were collected within 30 minutes in 500-μL eppendorf caps loaded with 50 µL RLT buffer (QIAGEN, USA) and 1% beta-mercaptoethanol (Sigma-Aldrich ...