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Citations for Qiagen :
251 - 300 of 526 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were incubated on a Thermomixer (37°C, 30 min, 500 rpm) before being subjected to MinElute (Qiagen) cleanup to extract tagmented DNA ...
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bioRxiv - Immunology 2021Quote: ... The mixture was pelleted by centrifugation at 10,000g for 30 minute and the supernatant was loaded onto a Ni2+ metal affinity column (Qiagen). The column was washed with buffer containing 30 mM imidazole and the protein was eluted in buffer containing 300 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... A total of 30 μL RNA solution was collected from each sample using a RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... to which were then added 180 µl of the kit’s Buffer ATL and 30 µl of Proteinase K (Qiagen) at 20 mg/ml ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... A total of 30 μl RNA solution was collected from each sample using an RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... the lysates were further processed using the Qiagen RNeasy kit with an additional DNAse treatment (30 min, Qiagen, 74106). RNA was eluted in 30 µl RNAse-free H2O and quantified with a spectrophotometer (NanoDrop ...
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bioRxiv - Microbiology 2020Quote: Each dissected compartment (legs and thoraxes) was homogenized individually 3 x 1 minute at 30 Hertz using TissueLyser (Qiagen) and glass beads (#11079110 ...
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bioRxiv - Microbiology 2021Quote: ... A total of 30 μL RNA solution was collected from each lung sample using RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 g for 10 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... 30 mg of each tissue was removed from RNAlater and homogenized in 700 μL of QIAzol lysis reagent (Qiagen) using the TissueRuptor homogenizer (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by ultracentrifugation (70,000 × g, 30 min) and supernatant was incubated with Ni-NTA resin (Qiagen) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: Tissue-gel samples were equilibrated in DNase buffer (750 ml) for 30 min and then incubated with RNase-free DNase1 (2.7 Kunitz units/µl, 50 µl) (Qiagen) in DNase buffer (450 µl ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins and bacterial membranes were separated by centrifugation (30 min, at 15,000 ×g) and the supernatant was applied to Ni-NTA beads (Qiagen) for 2h ...
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bioRxiv - Microbiology 2020Quote: ... Cells were mechanically disrupted by bead-beating for 2 × 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). The samples were incubated at −80°C for 10 min and at 95°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... untreated or metal-stressed bacteria (LB supplemented with 30 μM CdCl2) were harvested when they reached mid-log phase (OD600 0.7) and cells lysed in QiaZol (Qiagen) as described previously (37 ...
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bioRxiv - Microbiology 2020Quote: Tissues (up to 30 mg) were homogenized in RLT buffer and RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer's instructions ...
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bioRxiv - Physiology 2022Quote: Frozen liver tissue was manually crushed and 30 mg per sample was homogenized in 1mL of 2:1 chloroform:methanol via a TissueLyser II (Qiagen). Samples were stored at 4 °C overnight with agitation ...
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bioRxiv - Genetics 2022Quote: ... The lysates were cleared by centrifugation at 10,000 xg for 30 min and added to 1 ml of Ni-NTA agarose (Qiagen) pre-equilibrated with the same lysis buffer ...
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bioRxiv - Genomics 2022Quote: ... samples were incubated an additional 30 minutes with 20 µL Proteinase K and 4 µL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit ...
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bioRxiv - Biophysics 2020Quote: ... Debris was removed by centrifugation (20,000×g, 30 min, 4°C) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was tagmented with Tn5 transposase (25 μM) for 30 min at 55°C and purified using MinElute columns (QIAGEN).
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bioRxiv - Physiology 2020Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were purified with 1x volume of SPRIbeads as previously described and eluted in 30 μl EB buffer (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... and the tubes subjected to ten minutes of vigorous shaking at 30 Hz in a TissueLyser II machine (Qiagen). After subsequent DNA binding and washing steps in accordance with the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Tissues (up to 30 mg) were homogenized in RLT buffer and RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were homogenized at 25–30 Hz for 5 min using a tissue homogenizer (QIAGEN TissueLyzer II, Hilden, Germany). Samples were swabbed with sterile dual tip swabs (BD swubes ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM EDTA pH 8.0 at 50 ºC for 30 min followed by centrifugation then purification by QIA-PCR clean up (Qiagen). The 193 bp DNA from each round was amplified by KOD then Taq polymerase and purified by anion exchange chromatography using a MonoQ column (48 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was isolated by centrifugation for 30 min at 20,000 ×g and then incubated with a Ni2+-NTA column (QIAGEN) for 5h ...
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bioRxiv - Genomics 2021Quote: Tissue lysates were prepared from 30 pooled 2 dpf WT and MZdnmt3aa-/- mutant by homogenization using TissueLyser LT (QIAGEN) with zirconia beads for 10 s at 50 Hz ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4C ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Pathology 2021Quote: ... RNA from 30 mg tissue samples was extracted via the the RNeasy Plus Mini kit (Qiagen, Mississauga, ON, Canada) or the Nuclisens EasyMag using Specific Protocol B 2.0.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell debris was cleared by centrifugation for 30 min at 10,000 x g and passed over Ni-NTA agarose resin (Qiagen) pre-equilibrated with buffer A at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The lysed solution was centrifuged at 12,000 × g for 30 min and the supernatant was incubated with Ni-NTA agarose (Qiagen) at 4 □ for 3 hours ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The sonicate was centrifuged at 38,000 × g for 30 min and the supernatant was loaded on a Ni-NTA agarose column (2.5 × 5 cm, Qiagen) pre-equilibrated with buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 5 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2023Quote: ... samples were incubated an additional 30 minutes with 20 uL Proteinase K and 4 uL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit.
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bioRxiv - Microbiology 2023Quote: ... Cell lysate was cleared by centrifugation at 100,000 × g for 30 min at 4 ⁰C and then incubated with Ni2+-NTA agarose beads (Qiagen) for 30 min at 4⁰C ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 2 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Immunology 2022Quote: ... Lysate was cleared from debris by centrifugation at 30,000 g and 4°C for 30 min before being loaded onto a Ni-NTA Superflow cartridge (Qiagen) using an Äkta Explorer chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The combined tissues were submerged in 600μl of lysis buffer containing 30 μl of foaming reagent (Reagent DX, Qiagen) and 60μl of β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer in a 50 mL falcon tube ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Microbiology 2024Quote: ... Material was ruptured two times for 1 min at 30 Hz in a TissueLyser (Qiagen Inc., Germantown, MD, USA). Between and after these two steps of tissue disruption ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was cleared by centrifugation at 12’000 g for 30 min at RT and added to Ni-NTA agarose beads (Qiagen) equilibrated in lysis buffer ...