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Citations for Qiagen :
401 - 450 of 1284 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... His-Hcp was purified from the soluble fraction by NTA-resin chromatography (Qiagen). Purified protein was desalted with a PD-10 desalting column (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... The His-tagged nanobodies were purified by using Ni-NTA purification column (Qiagen) followed by desalting step using disposable PD-10 desalting columns (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... His-tagged SLFN11 (residues 349-901) was purified by Ni-NTA beads (Qiagen). All purified GST- or His-tagged proteins were dialyzed against a buffer containing 50 mM Tris-HCl and 150 nM NaCl and validated by Coomassie blue-stained SDSLJPAGE.
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bioRxiv - Biochemistry 2019Quote: ... The cleaved tag and protease were removed by Ni-NTA affinity chromatography (Qiagen) and CLOCK PAS-AB was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal antibody that recognizes the strep-tag epitope was purchased from Qiagen. Recombinant NifB expressed in E ...
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bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
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bioRxiv - Neuroscience 2021Quote: ... coli DH5α and purified with a EndoFree Plasmid Maxi Kit (QIAGEN). The plasmids were then digested with AflIII and XbaI (both NEW ENGLAND BIOLABS ...
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bioRxiv - Cell Biology 2021Quote: ... coli and plasmid DNA was isolated by Plasmid Maxi-Prep (Qiagen). MCF10DCIS.com-GFP cells were cultured overnight to approximately 80% confluence ...
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bioRxiv - Molecular Biology 2021Quote: ... coli DH5α-pir and purified with QIAprep spin miniprep kit (Qiagen) according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... coli DH5α and purified using QIAprep spin mini-prep kit (Qiagen). All cDNA constructs were verified by DNA sequencing and services ...
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bioRxiv - Microbiology 2020Quote: ... coli strains was purified using the QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... coli cells using the QIAprep spin miniprep kit (Qiagen, Hilden, Germany). Electrocompetent C ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli NEB5α and isolated by miniprep according to the manufacturers (Qiagen) protocol ...
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bioRxiv - Genetics 2020Quote: ... coli cultures was performed with the QIAprep Spin Miniprep kit (Qiagen) and was combined with FastPrep (MP Biomedicals ...
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bioRxiv - Genomics 2020Quote: ... coli culture following infection using the RNeasy Mini kit (Qiagen: #74106) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... coli (DH5α) and afterwards isolated with a mini-prep kit (Qiagen) according to the manufacturer’s instructions as described below ...
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bioRxiv - Microbiology 2020Quote: ... coli M15 containing plasmid pREP4 to produce the LacI repressor (Qiagen). These strains were grown in 1L LB broth at 37°C with aeration until the OD 600 nm was 0.6-1.0 ...
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bioRxiv - Plant Biology 2023Quote: ... coli cells using a miniprep kit from Qiagen (Germantown, MD, USA) following the recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... coli DH5α and isolated using Qiagen QIAprep Spin Miniprep Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Viral DNA and RNA were extracted with the QIAamp MiniElute Virus Spin Kit (QIAGEN, Chatsworth, CA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Real-time one-step reverse transcription quantitative PCR was performed with the QuantiTect Virus Kit (Qiagen, Foster City ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted from supernatant culture media using the QIAamp 96 Virus QIAcube HT Kit (Qiagen). E-gene expression was determined using the SensiFAST Probe No-Rox One Step Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from apical washes using the QIAamp 96 Virus QIAcube HT Kit (QIAGEN). The SARS-CoV-2 genome was amplified using a highly multiplexed tiling PCR reaction based on the ARTIC protocol (https://www.protocols.io/view/ncov-2019-sequencing-protocol-bbmuik6w ...
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bioRxiv - Microbiology 2020Quote: ... virus medium was aspirated and cells were harvested using 400 µL of RNAprotect Cell Reagent (Qiagen). RNA was extracted using the Qiagen RNAeasy Mini kit ...
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bioRxiv - Microbiology 2022Quote: ... The RNA of rescued virus strains was extracted with QIAmp DSP viral RNA mini kit (Qiagen), reverse transcribed and amplified with OneTaq one-step RT-PCR kit (NEB ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... RNAs were extracted from each amplified single virus using the QIAamp Viral RNA Mini Kit (Qiagen). Each gene segment was synthesized by gene specific primers (primers available upon request ...
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bioRxiv - Microbiology 2020Quote: ... using a QIAamp Mini Elute Virus spin kit for the viral RNA/DNA extraction (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... using a QIAamp Mini Elute Virus spin kit for the viral RNA/DNA extraction (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Qiasymphony automated platform was used to isolate total cellular RNA (DSP virus/pathogen mini kit (Qiagen). RNA was further processed in an in-house assay using primers of previously validated assays (44 ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from the virus sample with a QIAamp Viral RNA mini kit (QIAGEN). SARS-CoV-2 RdRP-2 gene primer probes sequences are as follows ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from each sample using the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany), and a semi-nested PCR assays was used for the detection of viral nucleic acids in each sample ...
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bioRxiv - Genomics 2021Quote: ... Remaining 140 µL of concentrated virus was processed according to QIAamp Viral RNA Mini kit (QIAGEN) protocol ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were extracted from specimens using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Hilden, Germany), QIAamp Viral RNA Mini Kit (Qiagen) ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: Total DNA was extracted from tissues using QIAamp MinElute Virus Spin Kit (57704, Qiagen, Venlo, Netherlands) or KingFisher Cell and Tissue DNA kit (97030196 ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s protocol for the QIAamp MinElute Virus Spin Kit (Cat. 57704, Qiagen, Hilden, Alemania). The resulting DNA for each sample was quantified with a Qubit fluorometer (Cat ...
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bioRxiv - Cancer Biology 2019Quote: ... 200nM siRNA/well were used for transfection using 5µl/well of Hi-Perfect (Qiagen) following manufacturer’s recommendation.
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bioRxiv - Biochemistry 2020Quote: ... but with an anti-His antibody (Qiagen, #34660 at a dilution of 1:5000) as primary ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
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bioRxiv - Plant Biology 2022Quote: ... Blocking of the membranes was performed in anti His HRP conjugate blocking buffer (Qiagen). After blocking at room temperature for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... After incubation with the primary antibody from QIAGEN (Penta-His Antibody, 1:1000 dilution) at RT for 1 h under shaking ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210). Proteins were eluted by 200 mM imidazole ...
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bioRxiv - Cancer Biology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210) as previously described (21) ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21 (DE3) (38) and purified using Ni-NTA affinity column (Qiagen), HiTrapTM Heparin HP column and HiTrapTM Q HP column (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21(BE3) and purified using Ni-NTA agarose chromatography (Qiagen) as previously described (Xu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells and purified by FPLC using a Ni-NTA column (Qiagen) and Heparin (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified by Nickel affinity chromatography (Ni2+-NTA-sepharose resin, Qiagen). His6-FUBI-eS30 WT and AA constructs were expressed in E ...
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bioRxiv - Bioengineering 2022Quote: ... coli and purified using a QiaPrep Spin Miniprep kit (Qiagen, Venlo, NL). All plasmids were subjected to Sanger sequencing to verify the presence of the correct sing guide RNA (sgRNA ...
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bioRxiv - Microbiology 2023Quote: ... coli was extracted with the MagAttract HMW DNA kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol for DNA extraction from Gram-negative bacteria ...