Labshake search
Citations for Qiagen :
451 - 500 of 1284 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... coli RNA was isolated using an RNEasy column (Qiagen, Germantown, MD, USA) followed by polyadenylation with an E ...
-
bioRxiv - Biochemistry 2023Quote: ... coli using CLC Genomic Workbench v9.01 (QIAGEN® CLC bio, Aarhus, Denmark). Furthermore ...
-
bioRxiv - Cell Biology 2023Quote: ... coli bacteria (XL1Blue strain or DH5α strain) using plasmid extraction kits (QIAGEN; Venlo ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmids were prepared from VectorBuilder Escherichia coli stocks using Plasmid Miniprep (Qiagen) and ampicillin selection ...
-
bioRxiv - Genomics 2020Quote: The DNA of VLPs was extracted following the manufacture’s protocol for the QIAampMinElute Virus Spin Kit (QIAGEN). The resulting DNAs were quantified (Qubit ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from the sucrose-purified virus using the Qiagen DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was extracted from mock and virus infected Vero cells using the RNeasy mini kit (Qiagen, Germany). Next ...
-
bioRxiv - Plant Biology 2020Quote: DNA from virus-infected plant tissues was extracted by DNeasy Plant Mini Kit (QIAGEN, Cat. No. 69104), and 500 ng of purified DNA was subjected to bisulfite treatment using EpiTect Plus DNA Bisulfite Kit (QIAGEN ...
-
bioRxiv - Microbiology 2022Quote: The viral RNA of the egg-passaged virus was extracted using QIAamp Viral RNA Mini Kit (Qiagen). The extracted RNA was then reverse transcribed to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) ...
-
bioRxiv - Immunology 2021Quote: ... Viral RNA from mice serum was extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... For quantification RNA was extracted from 100 μl of virus preparation using RNeasy kit (Qiagen, Manchester, UK) and analysed by reverse transcription and quantitative PCR (qPCR).
-
bioRxiv - Bioengineering 2020Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was extracted from cell-free virus-containing supernatants using the viral RNA extraction mini kit (Qiagen). cDNA synthesis was conducted using Superscript IV and the uni12-FluG primer (AGCGAAAGCAGG) ...
-
bioRxiv - Immunology 2021Quote: ... RNA was isolated from 200 μL sample using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). For the qRT-PCR assay ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The RNA from the escaped virus was isolated using a QIAamp viral RNA mini kit (Qiagen, #52904), and the full-length HA was amplified using gene-specific primers and the OneStep RT-PCR kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: Total virus-RNAs were extracted from SK.N.SH infected cells at MOI 0.5 using RNeasy minikit (Qiagen, #74104). ISRE reporter assay was performed in STING37 cells plated in 24 wells plate (2×105 cells/well ...
-
A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from the concentrated virus with the QIAamp Viral RNA Mini Kit (Qiagen, 52904). The sgRNA sequences found in the genomic DNA and viral RNA samples were amplified by PCR (Agilent ...
-
bioRxiv - Genomics 2022Quote: Viral RNA was extracted from 200 μl of clinical sample using QIAamp 96 Virus QIAcube HT (QIAGEN) according to the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2022Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
-
bioRxiv - Microbiology 2023Quote: ... and cleared supernatants stored at −80°C until virus titration/ spin through a QIA shredder column (Qiagen). Viral titers were expressed as TCID50 units per gram source tissue.
-
bioRxiv - Microbiology 2020Quote: ... were coated with 100 ng/well of mouse anti-Penta His BSA-free antibody (Qiagen) in PBS ...
-
bioRxiv - Biochemistry 2022Quote: ... a 1:2000 dilution of a mouse anti-Penta-His Alexa Fluor 647 conjugate (Qiagen) was used.
-
bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse anti-StrepII and anti-His antibodies were obtained from QIAGEN (catalog number 34850) and Genscript (catalog number A00186) ...
-
bioRxiv - Microbiology 2022Quote: ... BapR-CPD-His was affinity purified from cleared lysates using Ni-NTA agarose beads (Qiagen) with gentle rocking at 4°C for 2 hours ...
-
bioRxiv - Immunology 2022Quote: ... after which the cells were stained with anti-Penta-His-AF647 (1 µg/mL; Qiagen) for 30 minutes at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... the precipitates were subjected to Western blot analysis with anti-penta-His (1:2000, Qiagen) and anti-FLAG M2 (1:4000 ...
-
bioRxiv - Genomics 2019Quote: ... tBLASTp analyses (e-value < 0.001) were performed using CLC Genomics Workbench (CLC bio Qiagen). Protein sequences were defined as orthologs if they were reciprocal-best BLASTp hits having an e-value < 10−10 ...
-
bioRxiv - Neuroscience 2021Quote: ... Purification of C-terminal His6-tag-labeled GluN1 and GluN1ΔM4 by Ni2+-NTA agarose (Qiagen) chromatography was performed as in (Madry et al. ...
-
bioRxiv - Plant Biology 2019Quote: ... coli BL21 from pET16b-based plasmids and affinity purification via Ni-NTA (Qiagen), followed by specificity tests (Supplemental Figure 14).
-
bioRxiv - Microbiology 2020Quote: ... coli 10-beta cloning strain were purified using the standard QIAprep protocol (QIAGEN), then delivered to the Pseudomonas strain via previously published electroporation83 or conjugation84 protocols ...
-
bioRxiv - Biochemistry 2020Quote: ... coli and purified using Qiagen Ni+2 agarose as per vendor protocol (Qiagen). Recombinant proteins were dialyzed in demethylation buffer overnight at 4°C prior to use in enzymatic assays.
-
bioRxiv - Genomics 2022Quote: ... coli isolates from Germany was extracted using the QIAamp DNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... coli in LB Broth with 100ug/mL ampicillin using Qiaprep Spin columns (Qiagen).
-
bioRxiv - Microbiology 2019Quote: ... coli host JM109 and isolated with a commercial plasmid isolation kit (Qiagen Inc.). For cloning PCR fragments ...
-
bioRxiv - Developmental Biology 2020Quote: ... coli cells were isolated according to the QIAprep Spin Miniprep Kit protocol (Qiagen). A restriction reaction was then set up to linearize the plasmid DNA ...
-
bioRxiv - Developmental Biology 2022Quote: ... coli cells and positive colonies were collected using a Mini Prep kit (Qiagen) and sequenced using the U6 primer (Supplementary Table 1 ...
-
bioRxiv - Microbiology 2022Quote: ... coli strain TJ249 culture using the QIAGEN DNeasy Blood & Tissue kit (QIAGEN GmbH). The extracted genomic DNA was sequenced on the PacBio RS II platform using P6C4 chemistry at Macrogen (Tokyo ...
-
bioRxiv - Biophysics 2023Quote: ... coli and purified using a combination of Ni-NTA affinity (Qiagen, Hilden, Germany) and size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2024Quote: ... coli BL21 ClearColi cell expression system and purified by NiNTA-Agarose beads (Qiagen).
-
bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was isolated from Escherichia coli using a Spin MiniPrep kit (Qiagen) and transferred to X ...
-
bioRxiv - Microbiology 2020Quote: ... Total nucleic acids were extracted using QIAamp 96 virus Qiacube HT kit on QIAxtractor Automated extraction (Qiagen, US) following the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: The virus RNA was extracted from clinical samples using the QIAamp viral RNA mini kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: Viral RNA from cell culture supernatants was isolated as the manufacturer’s instructions using QIAamp MinElute Virus kit (Qiagen). Cells were suspended in TRI Reagent (Sigma Aldrich) ...
-
bioRxiv - Microbiology 2020Quote: Load Viral RNA was isolated from plasma and cerebral spinal fluid (CSF) using the QuantiTech Virus Kit (Qiagen). Viral loads were quantified by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA was extracted from 200 µl of the inactivated supernatant using the EZ1 Virus Mini Kit (Qiagen, Germany). As described previously ...
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from nasopharyngeal swab samples on the QiaSymphony platform using the Virus Pathogen Mini Kit (Qiagen). Libraries were prepared using the Illumina DNA Flex library preparation kit and sequenced on an Illumina MiSeq (V2 ...
-
bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from 140 µl of virus stock (SARS-CoV-2 VOC Delta, GISAID ID: EPI_ISL_15250227) using a QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: RNA from virus stocks was extracted with the QIAamp Viral RNA Mini Kit (Cat# 52904, QIAGEN, Hilden, Germany), and one-step cDNA synthesis was then performed with SuperScript IV RT Viral cDNA (Cat# 12594025 ...
-
bioRxiv - Immunology 2022Quote: ... 200 μL of nasal swab sample was extracted with the QIAamp 96 Virus QIAcube HT kit (Qiagen, Germany) on the QIAcube HT System (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... viral RNA was extracted from the virus stocks using the QIAamp viral RNA mini kit (Qiagen, Cat#74l36) and viral genomes were sequenced as described before [31].