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Citations for Qiagen :
401 - 450 of 1951 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: S2 cell transfections were carried out in 6-well plates using Effectene transfection reagent (Qiagen). Each well was transfected with 1 μg of the pAc5.1-EGFP:dFMRP and 1 μg of pAc5.1-mCherry plasmids containing DCR1 ...
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bioRxiv - Immunology 2023Quote: ... muris isolates (6 in total) were extracted via a DNeasy Blood and Tissue kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... corpus (segment 6-7) and cauda (segment 8-10) samples using QIAzol Lysis Reagent (Qiagen) following the manufactureŕs recommendation using a bead-based tissue homogenizer (Retsch ...
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bioRxiv - Microbiology 2024Quote: ... 1.5x105 Acanthamoeba castellanii cells were transfected with 6 μg of linearized plasmid using Polyfect (QIAGEN) in phosphate saline buffer (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... High speed supernatant was combined with 6 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) and stirred in a beaker for 1-2 hour(s ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted from cells in 6 cm dishes using the RNeasy kit (Qiagen # 74106) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with guide RNAs for Rme-6 knockdown using Polyfect Transfection reagent (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... AC7594-5’-CCGCCTATCCTCGTCATGAAC)andcontrolALG9primers(AC5067-5’-CACGGATAGTGGCTTTGGTGAACAATTAC;AC5068-5’-TATGATTATCTGGCAGCAGGAAA GAACTTGGG) (0.5 µM) and QuantiTect SYBR Green PCR master mix (Qiagen) on a StepOnePlus Real-Time PCR machine (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: RNA extraction from sorted CD8 T cell populations pooled from 5-7 mice (week 5 and 8 p.i.) was performed using the RNeasy Plus mini kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Cell free DNA was extracted from 850μl serum using the QIAamp Circulating Nucleic Acid Kit (Qiagen) and was quantified by TaqMan Real-time PCR (StepOneTM Plus Real-Time PCR System ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was loaded onto a column packed with Ni(II)-nitriloriacetic acid agarose (Qiagen) pre-equilibrated in 100 mL of binding buffer (50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM PMSF and the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose (QIAGEN) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads as per the manufacturer instructions (Qiagen, Hilden, Germany). Beads were washed ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted using the Qiagen QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), substituting carrier RNA with linear polyacrylamide (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were isolated from cell lysates or lung homogenates using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The complete amino acid sequences were aligned by the CLC Genomics Workbench software version 11.0.1 (QIAGEN) (default parameters with very accurate ...
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bioRxiv - Molecular Biology 2020Quote: ... the collected eluate was incubated with pre-washed nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen) for 90 min at 4°C to remove the His-tagged TEV protease ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from 2OD of cells using acid phenol and purified using RNeasy kit (Qiagen). Reverse transcription was performed on 750ng of RNA using SuperScript III First-Strand Synthesis SuperMix (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: HUVECs were transfected at 60% confluency with 10 nmol/L locked nucleic acid (LNA) GapmeRs (Qiagen) or small interfering RNAs (siRNAs ...
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bioRxiv - Microbiology 2022Quote: Viral nucleic acid was extracted from the tissue homogenates using QIAamp DNA mini kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was then extracted from human blood plasma using the QIAmp Circulating Nucleic Acids kit (Qiagen), eluted in 60-μl elution buffer (10 mM Tris-Cl ...
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bioRxiv - Microbiology 2021Quote: ... Total nucleic acid was extracted using the Qiagen QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), substituting carrier RNA with linear polyacrylamide (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted (MagMAX Total Nucleic Acid Isolation Kit, Life Technologies, Carlsbad, CA using a Qiagen robotic extraction station ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was first purified on a Ni–nitrilotriacetic acid (NTA) column (QIAGEN, Valencia, CA). The eluate was further purified on glutathione–Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Total nucleic acid extraction from nasopharyngeal specimens was performed using the QIAamp RNA Viral Kit (Qiagen), and two sets of primers were taken for two target genes (ie ...
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bioRxiv - Microbiology 2021Quote: ... Extracted nucleic acids were passed through the Allprep DNA/RNA Mini Kit (Qiagen Sciences, Maryland, USA) to separate RNA from DNA ...
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bioRxiv - Genomics 2020Quote: ... where it underwent nucleic acid extraction using the QIAamp Viral RNA mini kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... Nucleic acids were extracted from the homogenate using AllPrep DNA/RNA/miRNA Universal kit (Qiagen, Germany). The quantity and quality of DNA and RNA were analyzed using the NanoDrop 1000 spectrophotometer and its integrity verified by the Agilent 2100 BioAnalyzer.
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Microbiology 2022Quote: ... The unprotected nucleic acids were removed by adding 50 μL of 10x Turbo DNase Buffer (Qiagen), 5 μL of 2U/μL Turbo DNase (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were extracted from specimens using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Hilden, Germany), QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: Hippocampal DNA was extracted using an automated nucleic acid extraction platform called QIA cube HT (Qiagen) with a column-based extraction kit ...
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bioRxiv - Genomics 2023Quote: ... Cell free DNA was isolated from plasma using the Qiagen Circulating Nucleic Acids Kit (Qiagen GmbH). For 29 patients ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and then subjected to total nucleic acid extraction using QIAamp viral RNA mini kit (Qiagen, Germany) according to manufacturer’s instruction without addition of carrier RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The nucleic acid fraction was then added to the column of a RNeasy microkit (Qiagen # 74004) and the manufacturer’s protocol was followed ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleic acids were then precipitated using ethanol and genomic DNA was removed using DNase treatment (Qiagen). RNA was quantified using Qubit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was added to 3 ml of Ni-nitrilotriacetic acid (NTA) superflow resin (Qiagen) and the mixture was applied onto a gravity drip column ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing each target protein was loaded onto a Ni-NTA (nitrilotriacetic acid) column (Qiagen) which was pre-equilibrated with the corresponding Lysis Buffer ...
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bioRxiv - Microbiology 2024Quote: ... A volume of 200µl homogenate supernatant was used for nucleic acid extraction with the QIAcube (Qiagen) machine ...
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bioRxiv - Microbiology 2022Quote: ... Viral nucleic acids were extracted using the QIAMP® Viral RNA mini kit (Qiagen, Venlo, Netherlands) without addition of carrier RNA to the lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Purification was performed on nickel-nitrilotriacetic acid (Ni-NTA) agarose using the QIAexpressionist™ kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Total ribonucleic acid (RNA) from the hippocampus was isolated with the RNeasy mini kit (©QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: All DNA extractions were completed with the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114) or with the QIAamp® MinElute ccfDNA Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... WT and mutant proteins were initially purified using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAgen). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: Nucleic acid was extracted from each sample using QIAmp Viral RNA mini kits (Qiagen, Hilden, Germany) in accordance with manufacturer protocols ...
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bioRxiv - Physiology 2024Quote: ... RNA was reverse transcribed using miRCURY Locked Nucleic Acid (LNA™) Reverse Transcription (RT) kit (Qiagen) according to manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized suspension was then applied to a nickel-nitrilotriacetic acid agarose (Ni-NTA agarose, QIAGEN) column ...