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Citations for Qiagen :
4301 - 4350 of 4479 citations for 6 METHYL 2 OXO 4 THIOPHEN 2 YL 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was subjected to immobilized metal affinity chromatography using a 1 mL Ni-NTA agarose column (Qiagen, 30210) equilibrated with binding buffer ...
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bioRxiv - Physiology 2024Quote: ... The digest was run on an 1% Agarose gel (TAE buffer, 0.25 µg/ml EtBr) and the the QIAquick Gel extraction kit (Qiagen, 28704) was used to extract the pLV6 backbone according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized supernatant was collected by another round of ultracentrifugation (200,000 × g, 1 h) and applied to a Strep-Tactin resin column (Qiagen, USA) equilibrated with buffer A containing 0.025% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was treated with 40U Dpn I for 1 hour and was purified by QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixture was incubated at 37°C for 1 hour followed by purification using QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: ... 5 µl of each PCR product was run in a 1% agarose gel and the remaining 35 µls were purified using the QIAquick PCR Purification Kit (Qiagen) before being sent for sequencing (sequencing primers ...
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bioRxiv - Microbiology 2024Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... semimature SP thymocytes were isolated on BD FACSAria II and BD FACSAria Fusion cell sorters directly into RLT + 1% beta-mercaptoethanol (BME) buffer (Qiagen). Libraries were generated by the Emory Integrated Genomics Core (EIGC ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were separated on a 1% agarose gel and bands were excised using a QIAquick gel extraction kit (Qiagen). The purified PCR bands were cloned using the NEB PCR cloning kit following manufacturer’s instructions and transformed colonies were screened using colony PCR ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mm (0.3 g) Zirconia beads and 1 mL of InhibitEx buffer from the QIAmp Fast DNA Stool Mini Kit (QIAGEN #51604) in a 2 mL tube ...
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bioRxiv - Microbiology 2024Quote: Infected whole eyes were homogenized in 1 mL of sterile PBS using a Retsch Mixer Mill MM300 (Qiagen, Valencia, CA) at 33 Hz for 4 min ...
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bioRxiv - Immunology 2024Quote: Genomic DNA samples were prepared from heparinized peripheral blood or 1×106 Jurkat cells following the instructions of the QIAamp DNA Blood Mini kit (#51104; QIAGEN). Primers were designed with the help of Oligo Primer Analysis Software version 7 (Molecular Biology Insights) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR products were analysed by gel electrophoresis using 1% agarose gel and purified using QIAquick gel extraction kit (Qiagen, Hilden, Germany). The DNA concentrations of the gel-purified samples were quantified with Nanodrop OneC (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Total RNA from THP-1 cells were extracted using the RNeasy® Plus Mini Kit (Cat. No. 74134, Qiagen, Limburg, Netherlands) according to the manufacturer’s instructions at 24 and 96h p.i ...
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bioRxiv - Cell Biology 2020Quote: ... Amplicons were electrophoresed in 1% agarose gel to check for amplification and the size distribution of the library and then column purified (Qiagen, 28104). Once purified ...
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bioRxiv - Microbiology 2021Quote: ... Each gene segment was amplified using segment-specific primers and extracted from 1% gel slices using Qiagen Gel Extraction Kit (Qiagen, Germany). Purified gene segments were cloned into the plasmid pHWSccdB as previously done (27) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was isolated from P0 Hnf4a mutant and control kidneys using the RNeasy Plus Micro kit (Qiagen 74034) followed by mRNA was isolation with NEBNext Poly(A ...
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bioRxiv - Genomics 2019Quote: ... Amplicons were electrophoresed in 1% agarose gel to check for amplification and the size distribution of the library and then column purified (Qiagen, 28104). Once purified ...
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bioRxiv - Genomics 2019Quote: RNA was extracted from 1×106 mESCs according to the manufacturer’s instructions using RNeasy Plus Mini Kit (Qiagen, cat. No. 74134) after cellular homogenization using QIAshredder (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... Aliquots of RNA (1 µg) from each sample were reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The amplified products were analyzed onto 1% agarose gel (low EEO) and extracted and purified from the gel using the QIAprep Miniprep Kit (Qiagen, Netherlands). The 16S rRNA sequencing reactions were performed using 785F (5’-GGATTAGATACCCTGGTA-3’ ...
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bioRxiv - Immunology 2021Quote: ... 30mg of whole spleen RNA was extract via 30 second homogenization in RLT lysis buffer containing 1% β-mercaptoethanol per RNeasy kit (Qiagen). RNA was reverse transcribed into cDNA using iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from ∼1 x 107 cells using a modified version of the Gentra Puregene Cell Kit cell extraction protocol (Qiagen 158745). Integrity of genomic DNA and absence of contaminating RNA was confirmed via 1% agarose 1x TAE gel electrophoresis ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... podocytes were washed three times with warm 1× PBS and the viral DNA was extracted using DNeasy Blood & Tissue kit (Qiagen #69504) [26] ...
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bioRxiv - Plant Biology 2020Quote: ... and thiols and disulphides were extracted in 1 mL of ice-cold 0.1 M HCl using a Tissue-Lyser (Qiagen, Hilden, Germany) and two 3-mm glass beads (30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... frozen in liquid nitrogen for 1 minute and homogenised by shaking at 40 Hz in a Tissue Lyser LT (Qiagen, Germany). The volume of elution buffer AE was adjusted to 200 μL for engorged females ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid sgRNA CRISPR porthos was co-transfected (1 µg of total DNA per well) with Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted once with 350 μL Phenol:Chloroform:Isoamyl Alcohol (25:24:1) in a phase-lock tube (Qiagen cat. no. 129046) and centrifuged 5 min at 16,000xg followed by extraction with 350 μL chloroform ...
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bioRxiv - Neuroscience 2022Quote: DIV14 neurons treated with 1 µM glutamate for the times indicated in the text then harvested using the Qiagen RNeasy Mini kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR was performed using 20 miRNA primers (Supplementary Table 1) and miScript SYBR Green PCR Kit (Qiagen, Hilden, Germany). RNA U6 was used as the endogenous control ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 2-times and 1 μl was taken for each PCR reaction (20 ul) containing: 0.02 U HotStarTaq® DNA polymerase (Qiagen, Germany), 1 x PCR buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... These specific colonies were amplified by culturing in liquid LB-medium with ampicillin (1:1000) followed by plasmid isolation using QIAprep Spin Miniprep Kit (Qiagen #27104), according to the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... These cells were incubated overnight in MEM-α media free of FBS and supplemented with 1% P/S followed by collection of Supernatant (conditioned media) and total RNA extraction (Cat# 74134, Qiagen, Germany). The resulting samples were stored at −80°C until analyzed further in downstream applications.
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bioRxiv - Molecular Biology 2022Quote: ... In all samples disruption occurred in 1 min intervals to avoid heat generation derived from the TissueRuptor II (catalogue #9002755, Qiagen, MD)29.
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bioRxiv - Developmental Biology 2022Quote: ... Each of the three reaction pellets were allowed to resuspend in TE for 1 hour at room temperature then pooled into one tube and purified across three Qiaquick PCR purification columns (Qiagen, 28104), eluted in the provided Elution Buffer and combined ...
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bioRxiv - Immunology 2022Quote: ... macrophages were lysed with 300μL lysis buffer with 1% v/v β-mercaptoethanol (Themo #12183025) and passaged through a QIAshredder homogenizer column (Qiagen #79656). Total RNA was isolated (Themo #12183025 ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from WT and T1KO was extracted from naïve and inflamed samples 1 day post CFA injection using a RNeasy Mini Kit (Qiagen, Venlo, Netherlands). To quantify cutaneous TIMP-2 and TIMP-4 mRNA expression ...
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bioRxiv - Cancer Biology 2019Quote: ... Normal DNA was extracted from 1 ml of whole blood stored in PAXgene blood DNA tubes using the PAXgene Blood DNA Extraction Kit (Qiagen, CA) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... 350μL RLT buffer supplemented with 1% pure β-mercaptoethanol were added and the cell slurry was transferred onto a QIAshredder spin column (Qiagen). Subsequently ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... estimation of base-editing efficiency in the bulk population was performed as follows: ~1×106 cells were collected from which genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen 69506). This was followed by PCR amplification of the desired region using DreamTaq Polymerase (Thermo Fisher Scientific EP0705) ...
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bioRxiv - Genomics 2021Quote: ... PCR amplicons were run on a 1% agarose gel and then purified using the QIAquick Gel Extraction kit (Qiagen cat # 28706). 1μg of DNA was digested with HindIII (New England Biolabs cat # R0104S ...
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bioRxiv - Physiology 2020Quote: ... the samples were thawed and physically disrupted using a beadmill (1 min max speed) using a TissueLyser LT (Qiagen, Hilden, DE) and then exposed to an in-house phenol-chloroform extraction protocol including an extra chloroform step and several RNA washing steps ...
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bioRxiv - Immunology 2020Quote: ... B220+Fas+CD38- CD45.1+ or CD45.1-) were sorted from immunized recipients directly into RLT buffer supplemented with 1% β-ME (Qiagen, Hilden, Germany). mRNA was extracted with an RNeasy Micro Kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... amplicons were analyzed in 1 % agarose gel and bands corresponding to approximately 600 bp were purified with QiAquick gel extraction kit (Qiagen, USA). All the reactions were carried out in triplicates and pooled together during the gel purification ...
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bioRxiv - Immunology 2021Quote: ... Freshly harvested or in -80°C stored organ in 1 ml complete cell culture medium was homogenized using a metal bead at 25 Hz for 1 - 1.5 min by TissueLyser (Qiagen, Hilden, Germany). After centrifugation at 3500g for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and quantitative RT-PCR was performed using SYBR-based primers (Supplemental Table 1) and RT SYBR Green qPCR Mastermix (Qiagen, #330509) on a CFX96 Touch Thermal Cycler (Bio-Rad ...