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Citations for Qiagen :
4201 - 4250 of 4479 citations for 6 METHYL 2 OXO 4 THIOPHEN 2 YL 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: pAC5.1 vectors with CLIP-CRY and TIM-SNAP-HA were delivered into s2 cells using Effectene reagent (Cat# 301425, Qiagen) or TransIT-Insect Transfection Reagent (Cat# MIR6100 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from Achilles enthesis of 1-dpi pups or from tissue culture cells using the RNeasy Kit (Qiagen). Reverse transcription was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: The frozen tumors were minced to small pieces and suspended in buffer P1 (QIAGEN, 1 mL Buffer/100 mg tumor) supplemented with 100 μg/mL RNase A (Promega) ...
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bioRxiv - Genomics 2022Quote: ... The indexed PCR products were cleaned using 1 Volume AMPure XP beads on magnetic stand and eluted with EB buffer (QIAGEN) followed by quantification using a Qubit dsDNA HS Assay Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... Skin samples were taken by remote biopsy dart (1 mm wide by 20-25 mm deep plug) and preserved in RNAlater (Qiagen). Blood samples were taken opportunistically during veterinarian visits and preserved in EDTA tubes ...
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bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
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bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... protocol 1 in the RNAprotect Bacteria Reagent Handbook (Enzymatic lysis of bacteria) was followed using the RNeasy Plus Mini Kit (Qiagen) using TE buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... 200 mg of thawed and mixed faecal samples were added to 1 ml of InhibitEX Buffer (QIAGEN, GmbH, Hilden, Germany) followed by the beat-beating step in TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and 18 centimeters below seafloor (cmbsf) from station AC of Van Keulenfjorden (Supp. Table 1) using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... ~100 mg tissue was homogenized in 1 mL Qiazol and RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were run on 1 μg of RNA per sample to afford cDNA using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed on an Applied Biosciences 7500 Real-Time PCR system following the QuantiTect SYBR Green PCR kit protocols (Qiagen ...
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bioRxiv - Immunology 2021Quote: Heat-inactivated human serum was diluted 1:10 in phosphate buffered saline (PBS) and applied to a gravity polypropylene flow column (Qiagen) containing 1 mL of Protein G-sepharose resin (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from 1 mL anaerobic digester sludge was performed using the DNeasy PowerSoil DNA extraction kit (Qiagen, Valencia, CA). DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from a suspension of 1 x 106 PBMCs from each individual in the study using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3 ...
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bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). A first round of PCR was performed using primers GSP1 and P1 (First denaturation step ...
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bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... or 50 um sections (liver metastases) were cut and homogenized in 1 ml of AllPrep DNA/RNA Mini Kit (Qiagen) using a plastic pestle (Cole-Palmer #44468-23) ...
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bioRxiv - Bioengineering 2022Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto pools using the QIAsep 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation before sequencing on an Illumina MiSeq platform at the Duchossois Family Institute Microbiome Metagenomics Facility at the University of Chicago ...
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bioRxiv - Microbiology 2022Quote: ... DNA on the exterior of filtered capsids was digested for 1 hour at 25 °C with 20.3 units DNase (Qiagen: 79254) supplemented with 10 mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified by ethanol precipitation and 1% agarose gel purified using the QIAquick Gel Extraction Kit (Qiagen). Single-round E ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50 to 100 μl of packed worms from wild type and brd-1(null) using the RNeasy Mini Kit (74104; Qiagen) and QIAshredder (79654 ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from nearly 100 µl of tightly packed age-synchronized adult worm pellet collected in 1 ml TRIzol reagent provided by Qiagen RNeasy Mini kit (Cat ...
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bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
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bioRxiv - Neuroscience 2022Quote: ... At day 28 ASOs were added (final concentration of 0.2, 1 or 7.5 μM) and RNA was harvested (RNeasy mini kit [Qiagen, Cat#: 74106]) after 5 days of treatment ...
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bioRxiv - Microbiology 2022Quote: ... including either whole fecal pellets or 200-300 uL of contents diluted 1:10 (w/v) in sterile PBS using the MagAttract PowerMicrobiome Kit (Qiagen), and prepped DNA libraries as previously described (36) ...
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bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
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bioRxiv - Microbiology 2022Quote: ... an aliquot of the sample was scraped off the frozen stock and homogenized for 1 min at 25 Hz in PBS using a tissue lyzer (QIAGEN). Serial dilutions were prepared in PBS and plated on MacConkey Agar (OXOID ...
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... One zirconia ball and 500 μl of 100% methanol premixed with ribitol (20:1) were added and samples were subsequently homogenized in the Tissue Lyser (Qiagen) 3 min at 25 Hz ...
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bioRxiv - Plant Biology 2023Quote: Total cellular RNA was extracted from 8-day-old wildtype or mutant seedlings grown on 1/2MS medium with RNeasy Mini Kit and QIAshredder (Qiagen). Three independently prepared samples with RNA integrity number greater than 8.0 from each line were sequenced and analyzed at The Centre for Applied Genomics at SickKids Hospital (Toronto ...
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bioRxiv - Neuroscience 2022Quote: SARS-CoV-2 RNA was harvested from the cell medium at various time points post infection and stored in viral lysis buffer with RNA supplements (50 μl of sample medium in 560 μl of AVL buffer supplemented with 1% carrier RNA) (Qiagen). RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 10000g for 10 minutes and the supernatant was allowed to bind with 1 ml of Ni-NTA beads (Qiagen) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufactures protocol ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections collected for virology were disrupted in 1 mL of sterile PBS using the TissueLyser LT (Qiagen, Germantown, MD) at 30 Hz for 5 min twice ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries (1 ng) were tagmented with the prepared single-loaded Tn5 transposase and afterwards cleaned using MinElute PCR kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... To this 200uL of resuspended pellet we added 1 volume (200uL) of the QX1 buffer from the Qiaex2 DNA extraction kit (Qiagen) [56] ...
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bioRxiv - Synthetic Biology 2024Quote: ... Illumina sequencing-compatible Unique Dual Index adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library quality control was performed using Qubit and TapeStation and sequenced on Illumina MiSeq platform to generate 2 × 250 bp reads.
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bioRxiv - Systems Biology 2024Quote: ... and left brain hemisphere using the phenol-chloroform extraction method with 1 mL of Qiazol Lysis Reagent (Qiagen, Hilden, Germany), as previously described in Ederer et al ...
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bioRxiv - Molecular Biology 2023Quote: ... Worms were snap frozen in liquid nitrogen before being ground into a fine powder for 1 min by a stainless-steel bead in a TissueLyser LT (Qiagen) operating at 50 oscillations per second ...
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bioRxiv - Microbiology 2024Quote: ... while samples for RNA-based analyses were mixed at a 1:10 ratio of LifeGuard Soil Preservation (Qiagen, Hilden Germany) before being put on dry ice ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1 μg of RNA was used as input for cDNA synthesis reactions by using the Omniscript RT Kit (Qiagen) along with random hexamers ...
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bioRxiv - Molecular Biology 2024Quote: ... The fidelity of the PCR reaction was checked using a 1 % agarose gel and then the reaction cleaned up using a PCR clean up spin column kit (Qiagen). Finally the concentration of the purified PCR product was checked on a NanoDrop spectrophotometer (Thermo-Fisher).
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Microbiology 2024Quote: ... HsMX1 or MmMx1 and infected or not with IAV at MOI 1 were extracted using the miRNeasy Mini kit (Qiagen). RNA quality was analysed using a BioAnalyzer (Agilent ...
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bioRxiv - Microbiology 2024Quote: 0,5-2 × 106 cells were collected 8 h or 24 h following mock infection or infection with A/Victoria/3/75 infection at MOI 1 and total RNAs were isolated using the RNeasy kit with on-column DNase treatment (Qiagen). cDNAs were generated using 125 ng RNA (High-Capacity cDNA Reverse Transcription Kit using 250 nM oligodT instead of the provided random primers ...
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bioRxiv - Microbiology 2024Quote: ... corresponding to the three extraction protocols we used: (NM-1) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for mosquito DNA extraction ...
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bioRxiv - Microbiology 2023Quote: ... At the last passage parasite pellets with ∼1 × 108 parasites were collected for DNA isolation using DNeasy blood and tissue kit (Qiagen).
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bioRxiv - Genetics 2024Quote: ... and a negative control region within a regulatory region of the estrogen receptor substrate 1 (ESR1) without a predicted G4 motif (91) were PCR-amplified with HotStar Taq Polymerase (Qiagen). The PCR fragments were cleaned-up with Promega ReliaPrep DNA concentration kit and eluted in dH2O ...