Labshake search
Citations for Qiagen :
4101 - 4150 of 4234 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... amplicons were analyzed in 1 % agarose gel and bands corresponding to approximately 600 bp were purified with QiAquick gel extraction kit (Qiagen, USA). All the reactions were carried out in triplicates and pooled together during the gel purification ...
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bioRxiv - Immunology 2021Quote: ... Freshly harvested or in -80°C stored organ in 1 ml complete cell culture medium was homogenized using a metal bead at 25 Hz for 1 - 1.5 min by TissueLyser (Qiagen, Hilden, Germany). After centrifugation at 3500g for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and quantitative RT-PCR was performed using SYBR-based primers (Supplemental Table 1) and RT SYBR Green qPCR Mastermix (Qiagen, #330509) on a CFX96 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... total genomic DNA of FAFU-HL-1 was extracted using a Qiagen DNeasy Plant Mini kit following the manufacturer’s instructions (Qiagen, Valencia, CA). For ONT sequencing ...
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bioRxiv - Immunology 2021Quote: ... plasmid DNA was extracted for each sample from a 1 ml overnight culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue lysis was performed by re-suspending ∼30mg of sliced frozen tissue in a solution containing 1% β-mercaptoethanol in RLT buffer (supplemented with antifoam agent; ID 19088, Qiagen). Next ...
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bioRxiv - Genetics 2022Quote: ... Routine qPCR for miR detection and validation (see Supplementary Table 1 for miR sequences) was performed with the miScript PCR control set (catalog number 218380; Qiagen, Germany). The miScript™ miRNA PCR Array Human Serum & Plasma 384HC (Cat No ...
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bioRxiv - Pathology 2022Quote: ... tissue samples were homogenized in 1 ml of phosphate buffered saline with a metal bead using a TissueLyzer II (Qiagen GmbH). Viral nucleic acids were extracted from blood and homogenized spleen ...
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bioRxiv - Systems Biology 2022Quote: ... the samples were thawed and physically disrupted using a beadmill (1 min max speed) using a TissueLyser LT (Qiagen, Hilden, DE) and then extracted with phenol-chloroform including an extra chloroform step and several RNA washing steps ...
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bioRxiv - Microbiology 2023Quote: ... we used low-binding plastics and added 1 µl of 0.1 ng/µL tRNA carrier (i.e. spike water, Qiagen, Cat. No. 1068337) in each tube with stock eluted DNA ...
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bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were initially preserved with 1 mL of activated sludge (i.e., suspended phase) in 9 mL of LifeGuard Soil Preservation (Qiagen, Hilden Germany). This solution was thawed and centrifuged (16,000 g for 1-minute ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and resuspended in 350μl of Buffer RLT Plus supplemented with 1% (v/v) β-mercaptoethanol from the RNeasy Plus Mini Kit (Qiagen, 74134). RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent ...
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bioRxiv - Biochemistry 2022Quote: ... Memorial Sloan Kettering Cancer Center) at a dilution of 1:5,000) and His (mouse anti-penta-His antibody (Qiagen, cat. #34660) at a dilution of 1:10,000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration of the resulting His-tagged ß-CATENIN-1 fragments was quantified via quantitative Western blotting with a Penta-His antibody (Qiagen) and a recombinant His-tagged protein as external standard.
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated from C2C12 myoblasts transfected with GFP-empty vector or GFP-Δ(1-240)JPh1 using the RNeasy-plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a small amount of tissue (>25 mg) was added to 1 mL of TRIzol with a single 5mm stainless steel bead (Qiagen, Germany) and homogenised using a TissueLyser II (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following an incubation at 55°C for 1 hr in 450 µl of lysis buffer with proteinase K (QIAGEN, Hilden, Germany). Each colony was genotyped using twelve microsatellite loci69 ...
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bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was cut from a 1 % agarose gel and purified using the QIAGEN PCR purification kit (QIAGEN, Hilden, Germany). Afterwards ...
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bioRxiv - Biophysics 2024Quote: ... Cleavage products were separated on a 1% agarose gel and the linearised plasmid was purified with a QIAquick Gel Extraction Kit (Qiagen, #28706). Complementary synthetic DNA oligos coding for miR-34a target sites (Table S1) ...
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bioRxiv - Genomics 2023Quote: ... The digested vectors were run on 1% agarose gel and the target band was cut and harvested using QIAquick Gel Extraction Kit (QIAGEN 12362). The gel extraction products were further purified with QIAGEN MinElute PCR purification kit (QIAGEN 28004) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from BCBL-1-RTA at the indicated times following reactivation using the Dneasy blood and tissue kit following manufacturer guidelines (Qiagen 69581). 20ng of total DNA was used for qPCR using primers for the KSHV gene ORF57 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The digest was run on an 1% Agarose gel (containing ethidium bromide) and the pLV6 backbone was extracted with the QIAquick Gel extraction kit (Qiagen 28704) according to the manufacturer’s manual.
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bioRxiv - Molecular Biology 2024Quote: ... NEM was subsequently aspirated and the tissue homogenized for 1 min at 30 Hz using a TissueLyser II bead mill (QIAGEN, USA) in ice-cold homogenization buffer [10% glycerol ...
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bioRxiv - Bioengineering 2024Quote: ... IVD cells that were attached to the well were washed with 1% dPBS and lysed using RLT buffer (Qiagen, Hilden, Germany) and 1% mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: Cell pellets or lung lobes from individual mice were snap frozen in 350µl of 1:100 β-mercaptoethanol in the RLT Lysis Buffer from the RNAeasy mini plus Kit (Qiagen, 74134). Cell samples were thawed and vortexed while lung samples were homogenized for total RNA extraction intended for qPCR or bulk RNA-seq ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the RNA QuantiTect transcription kit (Qiagen, Cat # 205311). Approximately 100 ng of cDNA was used as a template for each qRT-PCR reaction using the PowerTrack SYBR Green supermix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... was run in a 1% agarose gel and the desired band (1.8 kb) was purified using QIAquick gel extraction kit (Qiagen, Cat. No. 28704) and used as a template for the transcription reaction ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from the supernatant of cells infected with Candid #1 (P0 and P11) using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) into the LB media and purified by Ni-NTA affinity chromatography (Qiagen, USA). The full length of ASSCP2 protein (ASSCP2-R ...
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bioRxiv - Developmental Biology 2020Quote: ... Japan) and 1 μL of the cDNA synthesis reaction mixture was used with the Platinum SYBR Green kit from Qiagen (Hilden, Germany). q-PCR was performed using a Rotor-Gene Q Real-time PCR machine (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were resolved on a 1% w/v agarose gel and purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: Adult Spadella cephaloptera were starved for three days and sacrificed together with developmental stages covering early zygotes to hatched 1 months old juveniles for RNA extraction by using a RNA extraction kit (Qiagen, Roermond, Netherlands). Additional adults and developmental stages were carefully anesthetized in 7.14% MgCl2 before fixation and fixed for 1 hour at room temperature for in situ hybridization experiments and treated as previously described (Wollesen et al ...
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bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were blocked with 5% milk in PBST (Phosphate buffer saline, 0.05% Tween 20) and incubated with monoclonal mouse anti-His antibody (Penta His, Qiagen, dilution 1:1000) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Compost was crushed using mortar and pestle under liquid nitrogen and ground to a fine powder for 1 min at 30 Hz using a tissuelyser II with a steel grinding jar pre-cooled with liquid nitrogen (Qiagen, Venlo, Netherlands). RNA was extracted using a modified method described by Patyshakuliyeva et al ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of strains IMX2300 and IMX2300-1 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Samples and the DNA extraction negative control (1 ml PBS) were processed for DNA extraction using the QIAamp DNA Microbiome kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Depletion of Host DNA protocol).
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked for 1 hour at room temperature either with a commercial blocking buffer (QIAGEN, anti RGS HIS6 HRP conjugate kit) or with 5% milk in 0.05% tween TBS buffer ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Each primer (3.5 μl) was combined with reverse-transcriptase reagent (1 μl) and SYBR® Green master mix (25 μl) (Qiagen, Valencia, CA). The thermal profile was as follows ...