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Citations for Qiagen :
4051 - 4100 of 4234 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C with agitation for six hours after which time 1 mL cell pellets were treated with RNAprotect (Qiagen). Cell pellets were washed in 0.5 mL PBS pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized supernatant was collected by another round of ultracentrifugation (200,000 × g, 1 h) and applied to a Strep-Tactin resin column (Qiagen, USA) equilibrated with buffer A containing 0.025% (w/v ...
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bioRxiv - Physiology 2024Quote: ... The digest was run on an 1% Agarose gel (TAE buffer, 0.25 µg/ml EtBr) and the the QIAquick Gel extraction kit (Qiagen, 28704) was used to extract the pLV6 backbone according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and homogenized via sonication at 20 kHz for 1 min before RNA isolation was performed using a RNeasy Mini Kit (Qiagen). For qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was subjected to immobilized metal affinity chromatography using a 1 mL Ni-NTA agarose column (Qiagen, 30210) equilibrated with binding buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 µl of each PCR product was run in a 1% agarose gel and the remaining 35 µls were purified using the QIAquick PCR Purification Kit (Qiagen) before being sent for sequencing (sequencing primers ...
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bioRxiv - Microbiology 2024Quote: Infected whole eyes were homogenized in 1 mL of sterile PBS using a Retsch Mixer Mill MM300 (Qiagen, Valencia, CA) at 33 Hz for 4 min ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were separated on a 1% agarose gel and bands were excised using a QIAquick gel extraction kit (Qiagen). The purified PCR bands were cloned using the NEB PCR cloning kit following manufacturer’s instructions and transformed colonies were screened using colony PCR ...
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bioRxiv - Immunology 2024Quote: ... semimature SP thymocytes were isolated on BD FACSAria II and BD FACSAria Fusion cell sorters directly into RLT + 1% beta-mercaptoethanol (BME) buffer (Qiagen). Libraries were generated by the Emory Integrated Genomics Core (EIGC ...
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bioRxiv - Microbiology 2024Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was treated with 40U Dpn I for 1 hour and was purified by QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixture was incubated at 37°C for 1 hour followed by purification using QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mm (0.3 g) Zirconia beads and 1 mL of InhibitEx buffer from the QIAmp Fast DNA Stool Mini Kit (QIAGEN #51604) in a 2 mL tube ...
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bioRxiv - Neuroscience 2024Quote: ... we added 1 µl of the spike-in mix provided in the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN) to the tube ...
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bioRxiv - Immunology 2024Quote: Genomic DNA samples were prepared from heparinized peripheral blood or 1×106 Jurkat cells following the instructions of the QIAamp DNA Blood Mini kit (#51104; QIAGEN). Primers were designed with the help of Oligo Primer Analysis Software version 7 (Molecular Biology Insights) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR products were analysed by gel electrophoresis using 1% agarose gel and purified using QIAquick gel extraction kit (Qiagen, Hilden, Germany). The DNA concentrations of the gel-purified samples were quantified with Nanodrop OneC (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Total RNA from THP-1 cells were extracted using the RNeasy® Plus Mini Kit (Cat. No. 74134, Qiagen, Limburg, Netherlands) according to the manufacturer’s instructions at 24 and 96h p.i ...
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bioRxiv - Cell Biology 2020Quote: ... Amplicons were electrophoresed in 1% agarose gel to check for amplification and the size distribution of the library and then column purified (Qiagen, 28104). Once purified ...
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bioRxiv - Microbiology 2021Quote: ... Each gene segment was amplified using segment-specific primers and extracted from 1% gel slices using Qiagen Gel Extraction Kit (Qiagen, Germany). Purified gene segments were cloned into the plasmid pHWSccdB as previously done (27) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was isolated from P0 Hnf4a mutant and control kidneys using the RNeasy Plus Micro kit (Qiagen 74034) followed by mRNA was isolation with NEBNext Poly(A ...
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bioRxiv - Genomics 2019Quote: ... Amplicons were electrophoresed in 1% agarose gel to check for amplification and the size distribution of the library and then column purified (Qiagen, 28104). Once purified ...
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bioRxiv - Genomics 2019Quote: RNA was extracted from 1×106 mESCs according to the manufacturer’s instructions using RNeasy Plus Mini Kit (Qiagen, cat. No. 74134) after cellular homogenization using QIAshredder (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... Aliquots of RNA (1 µg) from each sample were reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The amplified products were analyzed onto 1% agarose gel (low EEO) and extracted and purified from the gel using the QIAprep Miniprep Kit (Qiagen, Netherlands). The 16S rRNA sequencing reactions were performed using 785F (5’-GGATTAGATACCCTGGTA-3’ ...
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bioRxiv - Immunology 2021Quote: ... 30mg of whole spleen RNA was extract via 30 second homogenization in RLT lysis buffer containing 1% β-mercaptoethanol per RNeasy kit (Qiagen). RNA was reverse transcribed into cDNA using iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from ∼1 x 107 cells using a modified version of the Gentra Puregene Cell Kit cell extraction protocol (Qiagen 158745). Integrity of genomic DNA and absence of contaminating RNA was confirmed via 1% agarose 1x TAE gel electrophoresis ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... podocytes were washed three times with warm 1× PBS and the viral DNA was extracted using DNeasy Blood & Tissue kit (Qiagen #69504) [26] ...
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bioRxiv - Plant Biology 2020Quote: ... and thiols and disulphides were extracted in 1 mL of ice-cold 0.1 M HCl using a Tissue-Lyser (Qiagen, Hilden, Germany) and two 3-mm glass beads (30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... frozen in liquid nitrogen for 1 minute and homogenised by shaking at 40 Hz in a Tissue Lyser LT (Qiagen, Germany). The volume of elution buffer AE was adjusted to 200 μL for engorged females ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid sgRNA CRISPR porthos was co-transfected (1 µg of total DNA per well) with Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted once with 350 μL Phenol:Chloroform:Isoamyl Alcohol (25:24:1) in a phase-lock tube (Qiagen cat. no. 129046) and centrifuged 5 min at 16,000xg followed by extraction with 350 μL chloroform ...
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bioRxiv - Neuroscience 2022Quote: DIV14 neurons treated with 1 µM glutamate for the times indicated in the text then harvested using the Qiagen RNeasy Mini kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR was performed using 20 miRNA primers (Supplementary Table 1) and miScript SYBR Green PCR Kit (Qiagen, Hilden, Germany). RNA U6 was used as the endogenous control ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 2-times and 1 μl was taken for each PCR reaction (20 ul) containing: 0.02 U HotStarTaq® DNA polymerase (Qiagen, Germany), 1 x PCR buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... These specific colonies were amplified by culturing in liquid LB-medium with ampicillin (1:1000) followed by plasmid isolation using QIAprep Spin Miniprep Kit (Qiagen #27104), according to the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... These cells were incubated overnight in MEM-α media free of FBS and supplemented with 1% P/S followed by collection of Supernatant (conditioned media) and total RNA extraction (Cat# 74134, Qiagen, Germany). The resulting samples were stored at −80°C until analyzed further in downstream applications.
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bioRxiv - Molecular Biology 2022Quote: ... In all samples disruption occurred in 1 min intervals to avoid heat generation derived from the TissueRuptor II (catalogue #9002755, Qiagen, MD)29.
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bioRxiv - Developmental Biology 2022Quote: ... Each of the three reaction pellets were allowed to resuspend in TE for 1 hour at room temperature then pooled into one tube and purified across three Qiaquick PCR purification columns (Qiagen, 28104), eluted in the provided Elution Buffer and combined ...
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bioRxiv - Immunology 2022Quote: ... macrophages were lysed with 300μL lysis buffer with 1% v/v β-mercaptoethanol (Themo #12183025) and passaged through a QIAshredder homogenizer column (Qiagen #79656). Total RNA was isolated (Themo #12183025 ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from WT and T1KO was extracted from naïve and inflamed samples 1 day post CFA injection using a RNeasy Mini Kit (Qiagen, Venlo, Netherlands). To quantify cutaneous TIMP-2 and TIMP-4 mRNA expression ...
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bioRxiv - Cancer Biology 2019Quote: ... Normal DNA was extracted from 1 ml of whole blood stored in PAXgene blood DNA tubes using the PAXgene Blood DNA Extraction Kit (Qiagen, CA) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... 350μL RLT buffer supplemented with 1% pure β-mercaptoethanol were added and the cell slurry was transferred onto a QIAshredder spin column (Qiagen). Subsequently ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... estimation of base-editing efficiency in the bulk population was performed as follows: ~1×106 cells were collected from which genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen 69506). This was followed by PCR amplification of the desired region using DreamTaq Polymerase (Thermo Fisher Scientific EP0705) ...
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bioRxiv - Genomics 2021Quote: ... PCR amplicons were run on a 1% agarose gel and then purified using the QIAquick Gel Extraction kit (Qiagen cat # 28706). 1μg of DNA was digested with HindIII (New England Biolabs cat # R0104S ...
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bioRxiv - Physiology 2020Quote: ... the samples were thawed and physically disrupted using a beadmill (1 min max speed) using a TissueLyser LT (Qiagen, Hilden, DE) and then exposed to an in-house phenol-chloroform extraction protocol including an extra chloroform step and several RNA washing steps ...
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bioRxiv - Immunology 2020Quote: ... B220+Fas+CD38- CD45.1+ or CD45.1-) were sorted from immunized recipients directly into RLT buffer supplemented with 1% β-ME (Qiagen, Hilden, Germany). mRNA was extracted with an RNeasy Micro Kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.