Labshake search
Citations for Qiagen :
4001 - 4050 of 10000+ citations for Creatinine Serum Kit 4 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi, #130-097-678) and RNA extracted using RNeasy Micro Kit (Qiagen, # 74004). RNA library preparation ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was extracted using either a QIAGEN HiSpeed® Plasmid Midi Kit or Maxi Kit (QIAGEN Sciences, Germantown, MD, USA) as per the manufacturer’s instructions (HiSpeed® Plasmid Purification Handbook) ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
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bioRxiv - Genomics 2020Quote: RNA was prepared from the 4-dpi flash-frozen spleen tissues of each of the above 20 chicks after homogenization in Qiazol reagent (Qiagen, Manchester, UK), and subsequent preparation using RNeasy RNA isolation kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was applied to a nickel-NTA affinity column containing a total of 4 ml bed volume of nickel-NTA superflow resin (Qiagen, Venlo, Netherlands). After washing with a washing buffer containing 20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Biochemistry 2020Quote: ... at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA, Qiagen, Hilden, Germany) previously equilibrated with the corresponding lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... step for 15 min at 4°C to remove insolubilized material and the supernatant was incubated with Ni-NTA agarose beads (Qiagen, cat: 30230) for 2 hrs ...
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bioRxiv - Microbiology 2021Quote: ... Maize and soybean leaf and root tissue were pulverized for 2-min at a speed of 30 Hz with two 4-mm stainless balls in a TissueLyser II (Qiagen, Venlo, Netherlands). Total DNA was extracted from plant tissues with the OMEGA Mag-Bind Plant DNA Plus kit (Omega Bio-Tek ...
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bioRxiv - Genetics 2020Quote: ... DNA binding plate was incubated with 600 μL of liquid for 4 minutes at RT and centrifuged at 1,300 g for 5 minutes sequentially with PB buffer (Qiagen Cat. No. 19066), followed by PE buffer (Qiagen Cat ...
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with buffer containing Proteinase K (PK; Qiagen UK Ltd, Manchester, UK) (1 µg/ml PK ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was cleared by centrifugation at 18000 rpm at 4°C and the supernatant was loaded onto 5 mL Ni–NTA resin (Qiagen, Milan, Italy) equilibrated with binding buffer ...
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bioRxiv - Immunology 2020Quote: ... Vγ4+TCRγδ+CD3+CD44+CD27− and Vγ4−TCRγδ+CD3+CD44+CD27− populations from each mouse strain were sorted using a FACSAriaIII: populations into RNA protect (QIAGEN, Hilden, Germany). Total RNA was extracted from the sorted cells according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Bioengineering 2024Quote: Whole mouse aortas (n=18 per group) cleaned of perivascular tissue were perfused with sterile HBSS and stabilized overnight at 4◦C in RNAProtect reagent (Qiagen; Hilden, Germany) before long-term storage at -80◦C ...
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bioRxiv - Genomics 2024Quote: ... the Qiagen RNeasy Mini Kit was used according to Qiagen RNA Protect Reagent Handbook Protocols 4 and 7 with Appendix B on-column DNase digestion (Qiagen, Hilden, Germany). The RNA was assessed with UV-Vis spectrophotometry (Denovix DS-11 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Quality of the final library has been evaluated through Qubit 4 and fragments sizes were checked by migration using QIAxcel Advanced Instrument (QIAgen, Hilden, Germany). Fragments ready for sequencing sized between 280bp and 320bp ...
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bioRxiv - Systems Biology 2023Quote: ... a total of 600 µL of cold 2:1 methanol:chloroform was added and homogenized in a bead-based tissue homogenizer at 25 Hz for 4 minutes (TissuLyser II, Qiagen, Hilden, Germany). 200 µL each of water and chloroform were then added ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 20,000 x g for 45 minutes at 4°C and incubated with Ni-NTA agarose resin (Qiagen, catalogue number: 30230) pre-equilibrated with lysis buffer for 2 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... were weighed and homogenized in 1-10 µL/mg DMEM with a sterile glass bead at 30 Hz for 4 minutes using a TissueLyser (Qiagen, Germantown, MD) automated homogenizer ...
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bioRxiv - Cell Biology 2023Quote: ... collected into an Eppendorf tube and centrifuged at 11200 rpm at 4 °C for 6 min followed by pellet resuspension with 200 μl of QIAzol (Qiagen, Hilden, Germany). To isolate EC from the bottom layer ...
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bioRxiv - Plant Biology 2023Quote: ... leaf discs (30 mg) were collected 4 dpi and then flash frozen in liquid nitrogen and homogenized with the Tissue Lyser II (QIAGEN, Hilden, Germany) to make fine powder at 300 rpm for 1 min ...
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bioRxiv - Microbiology 2024Quote: ... Enzymatic lysis and proteinase K digestion of bacteria was performed following protocol 4 in the RNAprotect® Bacteria Reagent Handbook (Qiagen, Germany). Purification of total RNA from bacterial lysate using the RNeasy® Mini Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... the detection of N-gene of SARS-CoV-2 was performed by using the 2019-nCoV-2 RUO kit (Integrated DNA Technologies, Inc., Coralville, Iowa, USA) and One-Step RT-PCR Kit (QIAGEN® GmbH) on a Rotor-Gene Q real-time PCR cycler (QIAGEN® GmbH) ...
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bioRxiv - Genomics 2019Quote: Joint RNA/DNA extractions were performed with the RNA PowerSoil Total RNA Isolation Kit combined with the RNeasy PowerSoil DNA elution kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). About 5 g of sediment were used ...
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bioRxiv - Pathology 2019Quote: Taxonomy filtered from samples was determined by analysis of kit controls with no template and zymo sequencing controls of know diversity and abundance (the QIAamp DNA Microbiome Kit (Qiagen, Hilden, Germany) DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Neuroscience 2019Quote: ... and total RNA was isolated by using the RNeasy Lipid Tissue Mini Kit or RNeasy Plus Universal Mini Kit (Qiagen, Hilgen, Germany). Complementary DNA was synthesized by using the Omniscript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA and genomic DNA were extracted using, respectively, the InviTrap® Spin Universal RNA Mini Kit (Stratec Biomedical, Germany) and the DNeasy Blood & Tissue Kits (Qiagen, Germany) according to manufacturers’ instruction ...
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bioRxiv - Immunology 2021Quote: Total RNA from D+ and D- allografts or kidneys of MCMV infected- and uninfected-BALB/c mice was isolated and purified using the RNeasy Mini Kit and RNeasy MinElute Cleanup kit (Qiagen, Germantown MD) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Genetics 2020Quote: ... iris tissues were extracted using the Precellys 24 homogenizer and lysing kit (Bertin, Montigny-le-Bretonneux, France) together with the AllPrep DNA/RNA kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions including an on-column DNaseI digestion step using the RNase-free DNase Set (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted and purified as described in [9] using the Quick-RNA MiniPrep Kit (Zymoresearch, Irvine, CA, USA) and RNeasy MinElute Cleanup Kit (QIAGEN, Hilden, Germany). Per sample at least 150 ng of high-quality RNA were obtained ...
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bioRxiv - Molecular Biology 2021Quote: ... we extracted RNA using the RNeasy PowerSoil Total RNA Kit and DNA using the RNeasy PowerSoil DNA Elution Kit (Qiagen, Hilden, Germany). On each day of extraction (approximately every 5-15 samples) ...
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bioRxiv - Microbiology 2022Quote: Total RNA samples were extracted from swab samples and tissue samples from the macaques using the QIAamp Viral RNA Mini Kit and RNeasy Plus Mini Kit (Qiagen, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: RNAseq: mRNA was extracted from the iPSCs of the pig-tailed macaque using RNeasy Mini Kit extraction kit (Qiagen Inc. Valencia CA). Following cDNA synthesis ...
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bioRxiv - Genetics 2021Quote: ... and that of cultured cells was obtained using the AllPrep DNA/RNA/miRNA Universal kit or the miRNeasy mini kit (QIAGEN, Hilden, Germany). The samples were reverse-transcribed into cDNA with the High-Capacity cDNA reverse transcription kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and the sediments were used to coextract the total RNA and DNA using the RNeasy PowerSoil Total RNA isolation kit and the DNA elution kit according to the manufacturer’s protocol (Qiagen®, Valencia, CA). To eliminate potential contaminant DNA in RNA samples ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from spiked blood (1×104 to 1 CFU/mL) using MolYsis Basic5 DNA extraction kit (Molzym, Bremen, Germany) combined with QIAamp UCP Pathogen Mini Kit (Qiagen, Venlo, Netherlands) following manufacturer instructions ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... was extracted from whole cell RNA extracts (total RNA) using the miRNeasy Mini Kit and the RNeasy MiniElute Cleanup Kit (both Qiagen, Hilden, Germany). Extraction was performed according to the manufactureŕs instructions.
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bioRxiv - Genomics 2023Quote: ... The bacteriomes of two individuals were dissected and total DNA was extracted using a commercial extraction kit (DNeasy Blood & Tissue Kit, Qiagen, Hilden, Germany), following the manufacturer’s instructions for purification of total DNA from animal tissues ...
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bioRxiv - Cell Biology 2023Quote: ... Two different kits were used for this method depending on the primer set: Rotor-Gene SYBR® Green RT-PCR Kit (Qiagen, 204174) and SensiFAST™ SYBR® No-ROX One-Step Kit (BioLine ...
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bioRxiv - Microbiology 2023Quote: ... DNA of the cell pellet was extracted using the Invisorb Spin Universal Kit (Invitek Molecular GmbH, Berlin, Germany) and RNA was extracted using the QIAamp RNA Blood Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... followed by genomic DNA removal and cleaning using Qiagen RNase-Free DNase Set kit (cat#79254) and Qiagen Mini RNeasy™ kit (cat#74104) (Qiagen, Denmark). Integrity of the RNA samples was assessed using the Alegient 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... Clarified cell lysate was processed using a Qiagen RNeasy kit and in-column treatment with a DNase I kit (Qiagen, Germantown, MD) for purification of total RNA ...
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bioRxiv - Cell Biology 2023Quote: ... DNA from samples was extracted as per manufacturer’s protocol (Qiagen, DNeasy Blood and Tissue Kit 69504 and Qiagen Genomic DNA miniPrep Kit) and SNP array derived genotypes generated by Affymetrix UK Biobank AxiomTM Array kit by Cambridge Genomic Services (CGS) ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from Min6 cells using RNeasy Plus mini kit and from pancreatic islets using RNeasy Plus micro kit (Qiagen, ref 74034) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: Microbiome DNAs were extracted from the human samples on the same day of collection using the microbiome-specific kit (QIAamp DNA Microbiome Kit; Qiagen, Hilden, Germany). The DNA extraction was performed according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and colon (n = 35 each) and negative blank DNA extraction kit controls using the DNeasy PowerLyzer Powersoil kit (Qiagen, Germantown, MD, USA), with minor modifications to the manufacturer’s protocol as previously described51–53 ...
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bioRxiv - Microbiology 2023Quote: ... The MDA reaction was performed using the Qiagen REPLI-g Mini kit and purified with the QIAamp DNA Mini kit (Qiagen, Hilden, Germany). Next ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs and miRNAs were extracted from the hippocampal tissue using the RNeasy Mini Kit and the miRNeasy Micro Kit (Qiagen Inc., USA), respectively ...