Labshake search
Citations for Qiagen :
3951 - 4000 of 10000+ citations for Creatinine Serum Kit 4 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: PC9 and CRUK0764 cells were immediately centrifuged at 300 × g for 4 min to remove supernatant and add 350 µl RLT buffer (Qiagen, 79216) containing 1% β-mercaptoethanol (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Cys protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9-Cys wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were plated at a density of ∼100,000 cells/well in a 24 well plate and transfected the following day with 500 ng of midi-prepped endotoxin-free plasmid DNA (Qiagen, Germantown, MD, USA) using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA of these tissues was extracted using the RNeasy Mini kit or RNeasy Plus Micro kit (Qiagen, GmbH, Hilden, Germany) depending on the weight of starting material and treated with DNase ...
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bioRxiv - Genomics 2020Quote: Total genomic DNA was isolated from fresh leaves of elephant grass using QIAGEN® Genomic kit and purified from the gel by QIAquick Gel Extraction kit (QIAGEN). A total of 20 ug of high molecular weight DNA was used for Oxford Nanopore library and ultra-long reads library preparation (Supplementary Note) ...
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bioRxiv - Genomics 2020Quote: ... ~20 ng genomic DNA was amplified using a whole-genome amplification (WGA) kit according to the manufacturer’s instructions (Qiagen REPLI-g Mini Kit, Qiagen, Valencia, CA). The REPLI-g Kit is developed based on multiple displacement amplification (MDA) ...
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bioRxiv - Cancer Biology 2019Quote: DNA was isolated from remaining pellets from metabolite extraction using a DNA purification kit following the manufacturer’s protocol for tissues (QIAmp DNA Mini Kit, QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Genetics 2019Quote: ... DNA extractions were performed using either the Xtreme DNA Isolation Kit (for DNA Buccal Swabs; Isohelix, United Kingdom) or the Qiagen DNA Investigator Kit (for Transport Swabs; Qiagen, USA). DNA extractions were performed as recommended by the manufacturer except that the AW2 wash was performed twice for swabs extracted using the Qiagen kit in order to increase DNA purity.
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected by centrifugation (5 min at 500 x g) and RNA was isolated using RNeasy Mini Kit (RNeasy Mini Kit, Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... was isolated using Midi or Maxi kits for the validation screens gDNA was isolated using and Midi kits according to the manufacturer’s protocol (Qiagen, Hilden, Germany). PCR and sequencing were performed as previously described56 ...
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bioRxiv - Cancer Biology 2019Quote: DNA was isolated using the QIAamp DNA Blood Mini Kit and the DNeasy Blood & Tissue Kit (both from Qiagen, Hilden, Germany).
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using qScriptTM cDNA synthesis kits (QuantaBio, #95047-500) following RNA was isolated from 50- 100mg of sample using RNeasy mini kits (Qiagen, #74106) according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: RNAs were extracted from tumor tissues or cancer cell lines then reverse transcribed using Kit from RNAeasy mini kit (Qiagen 74106) and Vazyme (R223-111 01) ...
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bioRxiv - Cell Biology 2022Quote: ... In vitro mRNA transcription was performed from linearized plasmids using the mMessage mMachine SP6 Transcription Kit (Fisher, #AM1340) and purified using the RNeasy Mini Kit (Qiagen, #74104). RNA transcript sizes were confirmed using 1% agarose gels containing 0.05% bleach and Millennium RNA markers (Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was purified from whole individual inner ears using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany). DNase treatment was performed immediately after total RNA purification (RNase-free ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo #AM1975) and concentrated using the RNeasy MinElute Cleanup Kit (Qiagen #74204). Library preparation and sequencing were performed by the Genome Sequencing Facility at St ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples meeting these criteria were macro-dissected from serial unstained sections before extraction of genomic DNA using DNeasy® Blood & Tissue Kits for frozen samples or QIAamp DNA FFPE Tissue Kits for FFPE materials (Qiagen) following the manufacturer instructions.
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bioRxiv - Cancer Biology 2019Quote: DNA was isolated from the remaining pellets after metabolite extraction with a DNA purification kit according to the manufacturer’s protocol (QIAmp DNA Mini Kit for Tissues, QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Cancer Biology 2019Quote: ... All RNAs for the miRNA investigation were transcribed using miScript RT kits and subsequent qPCR performed using miScript Primer Assays and miScript SYBR kits (Qiagen, Courtaboeuf). Experiments were performed with at least triplicates for each data point ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted from faecal and necropsy samples using the GeneMATRIX stool DNA purification kit (EURx, Poland) and the DNeasy Blood and Tissue kit (Qiagen, Germany), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA samples from liver tissues were extracted by RNeasy Fibrous Tissue Mini Kit and RNeasy Mini Kit following to the manufacturer’s instruction (QIAGEN, Venlo, Netherlands). After quality check by electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... with the NucleoSpin® RNA preparation kit (Macherey–Nagel, Düren) and genomic DNA was quantitatively removed using RNase-Free DNase kit (Qiagen, Hilden) and Ambion™ DNA-free™ DNase kit (Thermo Scientific™ ...
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bioRxiv - Microbiology 2019Quote: ... sterile Dacron swabs (N = 11) and blank DNA extraction kits (N = 23)) using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, Germantown, MD) with minor modifications to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... From old liver tissues and clotted blood samples DNA was extracted manually using QIAamp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Physiology 2020Quote: ... an equal amount of ethanol was added and loaded on to the kit column and RNA further purified and cleaned using RNeasy kit (QIAGEN, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... From tissues and clotted blood samples DNA was extracted manually using QiaAmp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Developmental Biology 2020Quote: ... at and after 32-cell stage were extracted using Animal Tissue Direct PCR kit (FOREGENE) and those (~200) of unfertilized eggs and 2-cell embryos were purified using DNeasy® Blood & Tissue Kit (Qiagen). DNA fragments flanking target sites were amplified using primers listed in Supplementary Table S3 ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from peripheral blood of all 395 participants using the genomic DNA extraction kit QIAamp DNA Blood Mini Kit (Qiagen, Germany). The selected genomic DNA regions for the analysis of each gene included the most common reported SNPs (For NAT2 ...
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bioRxiv - Microbiology 2019Quote: ... 10 g of sediment each was used for DNA and RNA isolation with the PowerSoil DNA Isolation Kit (MO BIO) and RNA Powersoil™ Total RNA Isolation Kit (QIAGEN), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... The first replicate was extracted using the ISOLATE II Genomic DNA Kit as described in 2.1 and the second using the DNeasy Blood and Tissue Kit protocol (Qiagen, Valencia, CA) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of RNA per sample was reverse transcribed using a complementary DNA (cDNA) synthesis kit (QuantiTect reverse transcription kit, Qiagen, Germany). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Triparma retinervis and ‘Scaly parma’) was extracted using the DNeasy Plant Mini Kit or RNeasy Plant Mini Kit (Qiagen, Venlo, Netherlands), respectively ...
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bioRxiv - Physiology 2022Quote: Total RNA was isolated at the indicated time points and cDNA was synthetized using the RNeasy Mini Kit and the QuantiTect™ Reverse Transcription Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Skin and intestinal samples were handled aseptically and skin-associated DNA was extracted using a NucleoSpin Soil Extraction Kit (Machery-Nagel GmbH, Düren, Germany) while the intestine-associated DNA was extracted using MoBio DNA Extraction Kit (QIAGEN, Hilden, Germany) both as described (Hamilton et al. ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... for 3 h at 37°C under 5% CO2 and total cellular RNA was purified using PureLink™ RNA kit (Life Technology) or RNeasy Mini Kit (Qiagen) and the concentration and RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2023Quote: ... and the RNA-containing aqueous phase was subjected to a column purification kit using RNeasy Mini RNA extraction kit (Qiagen, USA). RNA was DNase treated ...
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bioRxiv - Genetics 2023Quote: Clones obtained from three independent conjugation assays were collected and subjected to genomic extraction using the kit DNeasy® Tissue Kit (Qiagen). DNA was mechanically fragmented using the Covaris method (DNA shearing with sonication) ...
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bioRxiv - Microbiology 2023Quote: Genomic bacterial DNA was isolated from overnight grown cultures (LB, 37°C) by the DNA Extraction kit (DNeasy Kit, Qiagen Germany) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: QIAamp DNA Blood Mini Kit (for whole blood, buffy coat and plasma) and QIAamp DNA Mini Kit for lesion scraping (Qiagen, Germany) were used for DNA extraction as per the manufacturer’s protocols except elution in 50μl elution buffer AE (10 mM Tris-Cl ...
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bioRxiv - Developmental Biology 2023Quote: ... From tissues and clotted blood samples DNA was extracted manually using QiaAmp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Immunology 2023Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Then the DNeasy PowerPlant Pro Kit protocol was followed and the extracted DNA was further purified with DNeasy PowerClean CleanUp Kit (Qiagen, Germany).
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from the thoracic muscle tissue of each individual using either the QIAamp 96 DNA Blood Kit or the DNeasy Blood & Tissue Kits (Qiagen, LLC). Libraries were prepared for low-coverage whole genome sequencing (2×150 bp reads ...
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bioRxiv - Microbiology 2023Quote: ... gene in lung tissues were determined using 2019-nCoV RUO Kit (Integrated DNA Technologies #10006713 and QuantiNova SYBR Green PCR kit (Qiagen #208052) according to the following cycling program ...
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bioRxiv - Cancer Biology 2024Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The DNA was extracted directly from the frozen samples using a commercial kit (DNeasy Power Soil Pro Kit, QIAGEN, Hilden, Germany), following the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted for total RNA sequencing using the Direct-zol RNA Miniprep Kit (ZymoResearch R2050) or the miRneasy Mini Kit (Qiagen 217004) according to manufacturer protocols ...