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Citations for Qiagen :
351 - 392 of 392 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Each complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Genomics 2020Quote: ... at which point we combined the resulting solution with 12 mL of PB buffer and followed standard MinElute PCR Purification Kit (Qiagen) protocol ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using the miScript II RT Kit with Hiflex buffer from 12 μL of extracted total RNA according to product manual (Qiagen). Expression of mRNA was quantified using QuantiTect SYBR Green PCR kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... from explants that had been cultured on RIM for 12 hours in the semi-synchronous LR induction system and purified using the RNeasy microkit (QIAGEN). Affymetrix ATH1 microarrays were hybridized with biotinylated cRNA targets prepared from the RNA samples according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The complete dataset of 455,774 sequences with coverage across the genome were aligned in CLCbio Genomics Workbench 12 (QIAGEN Bioinformatics) to the GenBank reference sequence NC_045512.2 ...
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bioRxiv - Systems Biology 2020Quote: ... We incubated these cultures on a shaker at 225 rpm and 32°C for 12-14 hours then pelleted the cultures by centrifugation and isolated plasmid using the EndoFree plasmid maxiprep kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... total RNAs were extracted from 12-day-old seedlings grown during long days using a RNeasy Plant Mini kit (Qiagen). Following RNA purification ...
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bioRxiv - Microbiology 2020Quote: ... then entered an extraction pipeline in which 12 samples were processed in parallel on the QIAvac 24 Plus Manifold (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... The input and immunoprecipiated DNA were reverse cross-linked at 60 degrees for 12 hours and then purified using a QIAquick PCR Purification kit (QIAGEN). The input DNA was diluted 1:100 ...
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bioRxiv - Genetics 2022Quote: ... and larvae (12 CRISPR-treated, 20 water-injected and 13 uninjected) selected for sequencing by using DNeasy Blood & Tissue Kit (QIAGEN) Purity and concentration of DNA samples were measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: DNA was isolated from PBMC and lymph nodes cells collected 12 weeks post-challenge using the QIAamp micro kit (Qiagen) following the manufacturer’s instructions and eluted in 50 μL of water ...
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bioRxiv - Microbiology 2021Quote: ... The protein complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Cell Biology 2021Quote: ... 10∼12 ml cell culture medium was subjected to extracellular RNA isolation using the exoRNeasy Maxi kit (Qiagen, Cat# 77164) with minor modifications ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Cancer Biology 2023Quote: Cancer cells treated with DMSO or vorinostat for 12 hours were used to extract total RNA using RNeasy Kit (Qiagen). RNA samples were profiled on the Mouse Genome 430 2.0 Array (Affymetrix ...
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bioRxiv - Physiology 2022Quote: Assessment of differential expression was conducted on the mapped reads of the 2 previously mentioned pipelines using 2 methods for comparison: the EDGE method with default settings in CLC Genomics Workbench 12 (QIAGEN) and EdgeR (version 3.34.0) ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from 12-week hearts of MyoAAV2-shCtrl or MyoAAV2-shAtf3 injected FS6KD mice using RNeasy fibrous kit (QIAGEN). Libraries were prepared for sequencing using a TruSeq Stranded mRNA sample prep kit (Illumina ...
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bioRxiv - Genetics 2023Quote: Genomic DNA of subclones from a 12-well plate along with 2 million parental cells were extracted using DNeasy Blood & Tissue Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags with VeraSeq polymerase (Qiagen). Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases.
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bioRxiv - Microbiology 2022Quote: ... The protein complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were induced with 100 μM IPTG at 12°C for 16 hrs and purified on a Ni2+ column according to manufacturer protocols (Qiagen). Proteins were eluted using 125 mM imidazole ...
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bioRxiv - Microbiology 2024Quote: ... Protein purity and stability was confirmed via SDS-PAGE using 12% polyacrylamide gels and Western blotting using Penta-His antibody (QIAGEN) (Fig ...
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from CD4+Foxp3YFP+ Tregs and CD4+Foxp3YFP− Tcons from WT-Foxp3YFPCre/YFPCre and Dgka-/-zfl/fl-Foxp3YFPCre/YFPCremice (10–12-week-old) using the RNeasy Plus Mini kit (Qiagen). RNA-sequencing libraries were prepared using Truseq stranded mRNA kit (Illumina ...
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bioRxiv - Systems Biology 2024Quote: Total RNA was extracted from endothelial cells after stimulation with ImP (100 nM) or control for 12 h using the RNeasy Mini Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from the anterior half of the body from 10-12 5 dpf larvae per sample at using the Rneasy Mini Kit (Qiagen). cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNAs were added 24 hours after seeding at the final concentration of 25 nM with 12 to 24 μL HiPerFect (Qiagen). On day 5 after transfection ...
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bioRxiv - Cell Biology 2024Quote: For optimisation of pericyte growth conditions RNA was collected from pericytes seeded at 1×105 in a 12-well plate using the RNeasy Mini Kit (74104, Qiagen) after 24 h in the appropriate GA medium ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Total RNA was extracted from iPSC-SNs seeded in 12-well plates using RNeasy Mini Kit (74106, Qiagen AB, Stockholm, Sweden) with DNase digestion (79256 ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from FFPE tumor cores (n= 12 samples) using RNeasy FFPE kits according to the manufacturer’s protocol (QIAGEN, Germantown, MD). RNA-seq libraries were generated using Truseq RNA Access Library Prep Kits (TruSeq RNA Exome kits ...
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bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Neuroscience 2023Quote: ... One well of a 60-80% confluent 12-well was harvested with 700 μl QIAzxol Lysis Reagent (Qiagen; Cat. No. 79306) for subsequent RNA extraction with RNeasy Mini Kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the Zymo Quick-DNA Universal Solid Tissue Prep Kit (No. D4069) following the manufacturer’s protocol (DNA from a few samples was also extracted with the Qiagen DNeasy Blood and Tissue Kit, No. 69504). DNA quality was assessed by electrophoresis on 3% agarose gels and DNA concentration was determined by dsDNA HS Qubit Assay (Molecular Probes by Life Technologies ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Total RNA of 12 WT and 11 Plcb1+/- mice from mPFC and HPC were isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen Düsseldorf, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 12-d-old seedlings grown at 22°C in LD conditions at ZT13 with the RNeasy® Plant Mini Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Pancreatic islets were isolated from a different subset of female mice at week 12 and stored in RNAlater (#76106, Qiagen, Hilden, Germany) for analysis by quantitative real-time PCR (qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... 3-5 μg of DNA was isolated from frozen cortex of 2- and 12-month-old mice according to the manufacturer’s protocol (DNeasy Blood & Tissue Kit, Qiagen, Cat No. 69504) and diluted with 10 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... S2 cells were transfected with 0.5 µg of DNA in a 12-well plate using the Effectene transfection kit (Qiagen, Cat. #/ID: 301425) and plated 48 hours after transfection on acid-washed No ...
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bioRxiv - Cell Biology 2021Quote: Drosophila S2R+ cells were transfected with 0.5 µg DNA (pAC-Gal4+pUASp-GEM, or pAC-LAMP1-GFP 77) in 12-well plate using Effecetene transfection kit (Qiagen, Cat. # / ID: 301425) and then were plated 48 hours after transfection on concanavalin A-coated glass coverslips 78 ...
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bioRxiv - Microbiology 2023Quote: ... The clean raw reads were de novo assembled into contigs using QIAGEN CLC Genomics Workbench (version 12; CLC Bio, a QIAGEN company Aarhus, Denmark). Subsequently ...