Labshake search
Citations for Qiagen :
101 - 150 of 392 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... and the plasmids were extracted using Qiagen Midi-prep (Qiagen, #12941). Lentiviral packaging was performed by co-transfecting library plasmids with pCMV601 VSV-G (Addgene ...
-
bioRxiv - Microbiology 2021Quote: ... Data was analyzed with CLC Genomics Workbench 12 (QIAGEN) by mapping the human reads onto the hg19 reference genome scaffold (GCA_000001405.28) ...
-
bioRxiv - Genomics 2022Quote: ... 12 µl proteinase K (14-22 mg/mL, Qiagen), and 12 µl β– mercaptoethanol) ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted from tumor tissue using DNA all prep kit (Qiagen).
-
bioRxiv - Molecular Biology 2020Quote: Total protein was extracted using the Qiagen All prep mini kit (Qiagen) after elution through the RNA spin column ...
-
bioRxiv - Neuroscience 2020Quote: ... All DNA constructs were prepared using Endotoxin-free Maxi Prep kits (Qiagen).
-
bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
-
bioRxiv - Biochemistry 2020Quote: ... we took 1 µg of plasmid (typically from a midi-prep (Qiagen)) and digested in a 20 µL reaction with 20 units of BamHI-HF (NEB ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The QIAprep spin mini-prep kit was purchased from Qiagen (Valencia, CA), the Wizard Genomic DNA Purification Kit was purchased from Promega (Madison ...
-
bioRxiv - Cell Biology 2022Quote: ... The plasmid was purified with the Endo-Free Mega prep kit (Qiagen) and sequenced by NGS using the approach described in Konermann et al35 ...
-
bioRxiv - Immunology 2020Quote: RNA was extracted using the All Prep DNA/RNA Mini kit (Qiagen) according the protocol provided by the manufacturer and RNA was eluted with 30 μl of RNase-free water ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNA was extracted using the DNA/RNA All prep kit (80204, Qiagen) following manufacturer instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... Plasmid was extracted from positive colonies using a Midi-Prep kit (Qiagen). Primers were designed to incorporate T7 promoter sequence and tracR sequence ...
-
bioRxiv - Cell Biology 2020Quote: ... were purified using the EndoFree Plasmid Maxi Prep Kit (Cat: 118379, Qiagen). Plasmids (20-30μg in PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmids were recovered by several midi-preps (Qiagen Plasmid Plus Midi Kit).
-
bioRxiv - Microbiology 2023Quote: ... faecalis using a modified protocol for the RNeasy Mini-Prep Kit (Qiagen). First ...
-
bioRxiv - Cancer Biology 2023Quote: ... Bacteria were expanded for maxi prep according to kit protocols (Qiagen #12163). Plasmid was isolated and used to create lentivirus ...
-
bioRxiv - Molecular Biology 2024Quote: ... and extracted with Jetgene mini prep kit (QIAprep Spin Miniprep Kit, QIAGEN). The correctness of constructions was verified by sequencing (Eurofins) ...
-
bioRxiv - Cell Biology 2022Quote: ... and plasmid DNA was isolated using Qiagen Mini-prep kit (Qiagen; 27115). Plasmid DNA was transfected into One Shot™ Stbl3™ Chemically Competent E ...
-
bioRxiv - Developmental Biology 2022Quote: ... scaled up and purified prior to transfection (Qiagen HiSpeed Maxi prep, 12663). For luciferase assays (Promega ...
-
bioRxiv - Neuroscience 2022Quote: ... We used the All Prep DNA/RNA/miRNA Universal Kit from Qiagen, https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/multianalyte-and-virus/allprep-dnarnamirna-universal-kit/?catno=80224 as per the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2024Quote: ... pUC57-Cap9-XbaI/AgeI/KpnI plasmid was purified by maxi prep (Qiagen). Plasmid was digested by XbaI/AgeI to release the 447 bp cap fragment which was gel purified and ligated with similarly cut pAAV-CD68-Cre-mut/p41-Cap9-7mer for the next round of AAV library production ...
-
bioRxiv - Cancer Biology 2020Quote: ... 11.5 μl of molecular grade H2O and 0.1 μl HotStarTaq DNA Polymerase (Qiagen, Cat# 203203). Reactions were incubated at 95°C for 15 minutes ...
-
bioRxiv - Immunology 2020Quote: ... 0.125 μl 20 μM SmarterR reverse primer (30) and 1 μl PCR-grade H2O (Qiagen). The amplification was performed in a thermal cycler (lid temperature 98°C ...
-
bioRxiv - Bioengineering 2023Quote: ... Transfection-grade plasmid DNA was purified using the QIAGEN plasmid plus Midiprep kit (QIAGEN, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... and plasmids were subsequently isolated using Qiagen mini-prep kits (Qiagen; Cat #: 27104).
-
bioRxiv - Molecular Biology 2021Quote: ... Small-scale isolation of plasmid DNA was performed with Mini-Prep kits (Qiagen); large-scale DNA plasmid purification was done with GenElute HP Endotoxin-Free Plasmid Maxiprep Kits (Sigma-Aldrich) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Bulk plasmid DNA was extracted using the Endofree Plasmid Maxi-prep kit (Qiagen) following manufacturer’s instructions and resuspended in 100µL of sterile water ...
-
bioRxiv - Immunology 2021Quote: ... The plasmid DNA was amplified and purified using a GIGA prep (Qiagen, USA).
-
bioRxiv - Physiology 2022Quote: RNA was extracted from fish liver using RNeasy Plus Mini Prep Kits (QIAGEN) following manufacturer protocols ...
-
bioRxiv - Genomics 2021Quote: Total RNA was extracted using All Prep DNA/RNA/miRNA Universal kit (Qiagen) 48 hours after mimic transfection ...
-
bioRxiv - Systems Biology 2021Quote: ... Amplified plasmid was recovered using a standard mini prep kit (Qiagen, Hilden, Germany) and sent for sequencing.
-
bioRxiv - Molecular Biology 2020Quote: ... Fractions were subjected to RNA prep with RNeasy Plus micro kit (Qiagen 74034) and a RT-PCR analysis with SYBR green (Biorad ...
-
bioRxiv - Neuroscience 2021Quote: ... Plasmid DNA was purified using the Qiagen Maxi Prep kit (Qiagen Cat#10023). AAVs were also packaged by Vector Builder into AAV2/9 ...
-
bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
-
bioRxiv - Microbiology 2020Quote: ... and the Fast Prep spin MPtm kit for soil (Mobio/Qiagen, Hildesheim, Germany). DNA quality was verified on an agarose gel ...
-
bioRxiv - Microbiology 2020Quote: ... Then selected colonies were cultured overnight for mini-preps following manufacture instructions (Qiagen). The resulting plasmids were sequence verified using a primer for the T7 promoter.
-
bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using a Qiagen mini-RNA prep kit (Qiagen, Germany) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... coli cells and positive colonies were collected using a Mini Prep kit (Qiagen) and sequenced using the U6 primer (Supplementary Table 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... and DNA was extracted using the Qiagen Maxi-prep-plus kit (Qiagen; 12981). Viral particles were generated using Lenti-X 293T cells (Takara ...
-
bioRxiv - Bioengineering 2022Quote: ... The resulting sample was passed through an RNeasy Mini Prep spin column (Qiagen), and then washed as per manufacturer’s instructions with 1 wash of RW1 Buffer ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmid isolation was carried out using QIAprep Spin mini-prep kit (QIAgen, 27104) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by the All-Prep Universal Kit for DNA and RNA isolation (Qiagen). DNA quality was tested by spectrophotometry and clean-up was performed using DNA precipitation as needed ...
-
bioRxiv - Physiology 2024Quote: ... at 10 U/μ purification using the QIAprep Spin MINI Prep Kit (Qiagen), the DNA was transcribed in vitro into RNA by T7 polymerase and then transfected into HeLa cells using Lipofectamine (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfection-grade plasmids of sequence-validated clones were obtained using the EndoFree Plasmid Maxi Kit (Qiagen) according to the manufacturer’s protocol.
-
bioRxiv - Evolutionary Biology 2021Quote: ... We subsequently purified mRNA from the RNA using the Oligotex mRNA prep kit (Qiagen) and synthesized cDNA from the resulting mRNA with SuperScript II RT (Invitrogen) ...
-
bioRxiv - Genetics 2020Quote: ... coli cultures by maxi-prep using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965) and quantified plasmid DNA using Qubit dsDNA HS Assay Kit (Thermo Fisher #Q32854) ...
-
bioRxiv - Neuroscience 2021Quote: ... encoded in the inducible bacterial expression plasmid pRK172 and purified by maxi-prep (Qiagen), was transformed into chemically-competent BL21 (DE3 ...
-
bioRxiv - Immunology 2022Quote: ... Positive colonies were expanded and plasmids purified using a plasmid Midi-prep kit (Qiagen) as per the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from the harvested cultures using RNeasy mini prep kit (QIAGEN). Genomic DNA was eliminated with TURBO DNA-free™ kit (Invitrogen) ...