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Citations for Qiagen :
351 - 400 of 2092 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Purification of His-tagged GA-MatryoshCaMP6s was performed with Ni-NTA agarose beads (Qiagen, cat. No: 30210). Before applying the lysate ...
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and His-tagged tIgM-Fc (tFcµ) protein was purified using Ni-NTA Agarose (Qiagen) and subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... The His-Sortase A 7M enzyme was purified away from the reaction using Ni-NTA resin (Qiagen) with 50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... The His-fused ZmbZIP75 was expressed in Transetta (DE3) cells and purified with Ni-HA beads (QIAGEN). The recombinant protein was incubated with biotin-labeled probe ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes were treated with 1:3000 dilution of Qiagen anti-Penta His HRP conjugate (Qiagen 34460) and excess antibody removed with 3 washes in PBST for 20’.
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bioRxiv - Biochemistry 2020Quote: ... Released affinity tags containing Flag-EGFP were removed from the mixture by a Ni-NTA resin (QIAGEN). The non-absorbed fractions were concentrated and subjected to a size-exclusion column chromatograph using a Superose6 Increase column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... reverse transcribed to produce cDNA (QuantiTect, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was generated using Omniscript (Qiagen). RT-qPCR was carried out using primers specific (Table S1 ...
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis (RT2 First Strand Kit, Qiagen), and Realtime PCR (RT2 SYBR Green qPCR Mastermixes ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was purified by spin column (Qiagen) and then quantified by real-time quantitative PCR (RT–qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... The RT2 PreAMP cDNA synthesis kit (Qiagen) was used with the Human Aging RT2 PreAMP Pathway Primer Mix ...
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bioRxiv - Biophysics 2020Quote: ... cDNA purified using QIAquick PCR purification (QIAGEN). Expression levels of HER2 ...
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bioRxiv - Immunology 2022Quote: ... using the Sensiscript cDNA synthesis kit (Qiagen). This cDNA synthesis primer also includes a 21 nucleotide UMI that is present in the amplified product and is used in downstream computational analysis ...
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bioRxiv - Biochemistry 2023Quote: ... falciparum cDNA and cloned into pQE30 (Qiagen) and the plasmid encoding the H ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was generated using Omniscript (Qiagen) as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... and reverse transcription to cDNA (Omniscript, Qiagen) was conducted using 500 ng RNA per sample ...
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bioRxiv - Neuroscience 2024Quote: ... SK-N-BE(2) cells and iNeurons with an RNeasy kit (Qiagen) using the manufacturer’s protocol including homogenization of samples with QIAshredder spin columns (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with Penta His Alexa Fluor 647 Conjugate (Qiagen, UK). Hits (duplicate AF647 positive spots ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... His-SFP1 was purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen). Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... All fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using anti-His (Qiagen) and anti-strep (MilliporeSigma ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged talin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged vinculin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg of extracted RNA from each sample was used for the cDNA synthesis (cDNA synthesis kit, Qiagen, Germany) according to the suppliers’ protocol ...
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bioRxiv - Microbiology 2024Quote: ... Conversion of total mRNA to cDNA was performed using a High-Capacity cDNA Reverse Transcription Kit (Qiagen, Hilden, Germany).
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bioRxiv - Biophysics 2021Quote: ... Amplicons from candidate clones were gel-purified (Qiagen 28704) and Sanger sequenced ...
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bioRxiv - Molecular Biology 2021Quote: ... The expressed EcoCas3-tag fusion proteins were purified using nickel affinity resin (Ni-NTA, Qiagen, Hilden, Düsseldorf, Germany). To remove tags ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... IL2-Rα ectodomain was produced with C-terminal 6xHis tag and purified on Nickel-NTA spin columns (Qiagen) according to manufacturer instructions.
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bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl of cDNA (total volume after cDNA synthesis: 20 µl) was analyzed by quantitative PCR on a Rotorgene Q device (Qiagen) employing the QuantiTect SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... were used to amplify cDNA fragments under standard PCR conditions from cDNA and cloned into the pDrive cloning vector (Qiagen) as previously described (Modrell et al. ...
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bioRxiv - Molecular Biology 2023Quote: We performed qRT-PCR to determine the optimal number of PCR cycles for cDNA amplification to generate a sufficient amount of cDNA for library construction (cycler: Rotor-Gene-Q, Qiagen, Germany ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was isolated and purified from cultured cells using a standard commercial RNA prep kit (#48500, Norgen) and 1 μg of RNA was reverse transcribed to cDNA using a cDNA synthesis kit (#205314, Qiagen). 10 ng of cDNA was used for quantitative PCR by SYBR Green ...
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bioRxiv - Microbiology 2024Quote: cDNA was generated from 1 µg of RNA using qScript cDNA SuperMix (QuantaBio) and the cDNA was purified using a QIAquick PCR Purification Kit (Qiagen). 2 µL of cDNA was used in a PCR reaction with primers specific to EF_B0058 and EF_B0059 (Table S2) ...
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bioRxiv - Biophysics 2021Quote: ... gliding motility assays were performed with specific immobilization of motors on the surface via penta-His antibodies (34660, Qiagen) as previously described[64] (Figure S4) ...
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bioRxiv - Molecular Biology 2022Quote: ... or Renilla-HA-NOD2 (750 ng/well) and His-FLAG-CAD (250 ng/well) using Effectene Transfection Reagent (Qiagen). The following day ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...