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Citations for Qiagen :
451 - 500 of 2092 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and purified His-Cdk9 (1 μM) in total volume of 300 μl were added to 50 μl of Ni-NTA agarose (Qiagen) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Tris–HCl 20 mM (pH 7.5)) by sonication and the His-tagged proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole in buffer A but complemented by ATP 1 mM + MgCl2 3 mM in the case of the Vibrio cholerae helicase ...
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bioRxiv - Microbiology 2024Quote: ... Protein purity and stability was confirmed via SDS-PAGE using 12% polyacrylamide gels and Western blotting using Penta-His antibody (QIAGEN) (Fig ...
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bioRxiv - Microbiology 2024Quote: ... GnTi expressed His-tagged gp140s and gp120s were purified from the harvested and clarified supernatant using Ni-NTA agarose beads (Qiagen) following manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: To determine bispecificity of bsAbs his-tagged HCV E2 (3 μg/mL in Tris-buffered saline (TBS)) was immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Culture supernatant containing His-tagged NTD protein was harvested 4 days after transfection and was purified using Ni-NTA resin (Qiagen). Spike protein was further purified with a Superose 6 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 8.0) ...
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Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64bioRxiv - Biochemistry 2024Quote: ... The recombinant hexa-His-tagged proteins were purified individually from corresponding soluble fractions by affinity chromatography with Ni-NTA agarose resin (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the QIAquick PCR Purification kit (QIAGEN, cat. n° 28104), and digested DNA products were extracted from the agarose gel using the QIAquick Gel Extraction kit (QIAGEN ...
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The alopecia areata phenotype is induced by the water avoidance stress test in cchcr1-deficient micebioRxiv - Genetics 2020Quote: ... RNA samples were purified by an RNeasy MinElute kit (QIAGEN N N.V., Venlo, Netherlands).
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bioRxiv - Cell Biology 2024Quote: ... treatment or remission serums (n = 4 repeats/condition) using the RNEasy plus kit (Qiagen). Libraries were generated with the KAPA mRNA HyperPrep Kit (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA (1 μg) was used for cDNA synthesis using the Quantitate Reverse Transcription kit for cDNA Synthesis for PCR (Qiagen, Germantown, md). Real-time PCR was performed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA synthesized with miScript II RT kit (Qiagen) using miScript HiFlex buffer ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using Quantitect Reverse Transcription Kit (QIAGEN) from 200 ng isolated mRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA is made with QuantiTect Reverse Transcription Kit (Qiagen). qPCR is performed to quantify the reporters (27 ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was performed with Omniscript RT Kit (Qiagen). qPCR was carried out using Power SYBR Green PCR Master Mix (ThermoFisher SCIENTIFIC ...
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bioRxiv - Physiology 2020Quote: ... and converted into cDNA using Omniscript RT kit (Qiagen). The Icelandic rock ptarmigan genome (Kozma et al. ...
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using Quantitect Reverse Transcriptase Kit (Qiagen). Quantitative RT-PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was purified with MinElute Gel Extraction Kit (QIAGEN). Obtained cDNA libraries were sent for high-throughput sequencing.
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bioRxiv - Microbiology 2020Quote: ... cDNA was cleaned using QIAquick PCR Purification Kit (Qiagen). Oligos to amplify gyrB ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and cDNA was synthesized using Reverse Transcription Kit (Qiagen). Quantitative real time PCR was performed using SYBR Green with QuantiTect Primers (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized using QuantiTect Reverse Transcription kit (Qiagen). Gene expression was quantified using TaqMan gene expression assays (Thermo Fisher ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized with Quantitec Reverse Transcription Kit (Qiagen) according to manufacturer’s instructions and diluted 10 times for RT-qPCR ...
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bioRxiv - Genomics 2022Quote: ... cDNA was eluted in 10ul of buffer EB (Qiagen). Then half of the contents of each well were transferred to a second 96 well plate ...
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bioRxiv - Genetics 2022Quote: ... Amplified cDNA was purified using MinElute PCR Kit (Qiagen) and eluted in 30 μL of EB ...
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bioRxiv - Genetics 2020Quote: ... cDNA was prepared by using Omniscript RT kit (Qiagen) primed with Oligo-dT on total RNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Resulting cDNA was concentrated using Minelute PCR purification (Qiagen) and 700 ng used for in vitro transcription/translation using TnT T7 Quick for PCR DNA (Promega ...
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using Quantitect Reverse Transcriptase Kit (Qiagen). Quantitative RT-PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was purified using a PCR purification kit (Qiagen) and the concentration was measured using NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared using RT4 First Strand Kit (Qiagen). qPCR was carried out on a StepOnePlus (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... The resulting cDNA was cleaned using MinElute columns (Qiagen). Second strand synthesis was performed using the NEBNext Ultra II second strand synthesis kit (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA libraries were generated (Qiagen Total Stranded RNA). cDNA libraries were sequenced using NextSeq 550 using a 75bp x 8bp x 8bp setup ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were purified using QIAquick Nucleotide Removal kit (Qiagen), vacuum dried and resuspended in 6 μl formamide dye (95% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... and transcribed to cDNA using Omniscript Reverse Transcriptase (Qiagen) and a 1:1 mixture of random hexamers and oligo dT as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... Double‐stranded cDNA was purified on QIAquick columns (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2022Quote: ... cDNA was prepared with the OmniScript RT Kit (Qiagen) and stored at −20° C until gene expression analysis using real-time PCR ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen), and RT-qPCR was performed using SYBR GREEN PCR master Mix (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was produced by Omniscript reverse transcription kit (QIAGEN). The resulting cDNA was diluted in nuclease-free water and stored at −20°C ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using Quantitect Reverse Transcription kits (Qiagen) and qPCR was carried out using the following program ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using miRCURY LNA RT Kit (Qiagen). Quantitative real-time polymerase chain reaction was performed using miRCURY LNA SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized using Quantitect Reverse Transcription kit (Qiagen). Transcript quantification was done using Applied Biosystems Taqman probes and ABI 7500 real-time quantitative PCR system ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was produced using the Omniscript kit (QIAGEN). qRT-PCR was performed using the EvaGreen kit (Axon ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was obtained with QuantiTect Reverse Transcription Kit (Qiagen) following manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was diluted in 250ul EB buffer (Qiagen, #19086) and stored at -20°C degrees ...
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bioRxiv - Immunology 2021Quote: Conditioned medium of HEK293 cells producing recombinant sema3A fused with 6xHistidine tag in C-terminal was collected and purified using Ni-NTA agarose beads (QIAGEN). The protein activity was assessed using the cytoskeleton collapse assay (19 ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated before (T1) and after (T2) the sample-tag staining procedure using the RNeasy Mini kit (Qiagen) and RNA quality (RNA integrity number ...
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bioRxiv - Systems Biology 2022Quote: ... The IL2Rα ectodomain was generated to include a C-terminal 6xHis tag and then purified on Nickel-NTA spin columns (Qiagen) according to manufacturer instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags with VeraSeq polymerase (Qiagen). Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases.
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bioRxiv - Cell Biology 2024Quote: ... SF was transfected with 12.5 nM antisense LNA GapmeRs CBP (Qiagen, Sequence: 5′-GCG GCG ATC CTT TAG A-3′) or p300 (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Clones were validated by plasmid purification (QIAprep Spin Miniprep Kit, Qiagen) and sequencing using the L4440 seq RV primer.