Labshake search
Citations for Qiagen :
351 - 400 of 1315 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing the His6-Nsp15 was loaded onto the Ni-NTA agarose column (Qiagen). The nickel column was extensively washed with wash buffer (25 mM HEPES-NaOH (pH 7.5) ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was purified from samples containing more than 500,000 cells using an RNeasy kit (Qiagen) following the protocol without the optional on-column DNase digestion step ...
-
bioRxiv - Genetics 2024Quote: ... ChIP-DNA was treated with 250 μL 1X TE containing 100 μg RNase A (Qiagen) and 5.0 μL of 20 mg/mL Proteinase K (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: Right apical lobes were collected into 2mL tubes containing 0.75mL of Qiazol lysis reagent (Qiagen) and 3mm glass beads ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatant containing PqsE was incubated with 1.5 mL of previously washed Ni-NTA resin (Qiagen) and mixed at 4 °C for 1 h ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: Initial screens were performed using Nucleix and Protein Complex suites (Qiagen) in a sitting-drop setup ...
-
bioRxiv - Microbiology 2021Quote: ... the proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole ...
-
bioRxiv - Biochemistry 2020Quote: ... E2 and polyPRM5 proteins were purified using Ni NTA-agarose (Qiagen) followed by cation exchange (Source15S ...
-
bioRxiv - Cancer Biology 2020Quote: ... The bacterial His-tagged proteins were purified under denaturing conditions (Qiagen) and refolded by dialysis as described previously (22) ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Plant Biology 2020Quote: GRF5 protein sequences were aligned using CLC DNA Workbench 8.0 (Qiagen) with the following settings ...
-
bioRxiv - Immunology 2021Quote: ... The proteins were captured using Ni-NTA (Qiagen, Germantown, MD, USA), washed in EQ buffer (25 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2021Quote: ... Fusion proteins were purified from clarified lysate by Ni-NTA (Qiagen) affinity chromatography ...
-
bioRxiv - Biophysics 2020Quote: ... Proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen) and the histidine tag was subsequently removed by thrombin digestion (10 U/mg of protein) ...
-
bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
-
bioRxiv - Molecular Biology 2022Quote: ... proteins in the supernatants were purified using Ni-NTA beads (Qiagen) according to (62) ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein was purified by FPLC using a Ni-NTA column (Qiagen), PBE-94 column (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... and RNA was extracted with DNA/RNA/Protein Mini Kit (Qiagen). The quality and quantity of RNA was assessed using the Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies) ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was purified from culture supernatant with Ni-NTA (Qiagen), biotinylated with in-house purified BirA enzyme ...
-
bioRxiv - Microbiology 2019Quote: ... Initial crystallization conditions were obtained with the Protein Complex Suite (Qiagen) screened by sitting drop-vapor diffusion at 18 °C using a Mosquito robot and were optimized in hanging drops by mixing 1 μL of the sample with 1 μL of the precipitant ...
-
bioRxiv - Cell Biology 2019Quote: ... The His-tagged protein was purified using Ni-NTA resin (Qiagen) following the manufacturer’s instructions and was used for rabbit immunization ...
-
bioRxiv - Genomics 2021Quote: ... The proteins were bound to a Ni-NTA sepharose beads (Qiagen), extensively washed with binding buffer and eluted in buffer containing 50 mM Tris-HCl pH 8 ...
-
bioRxiv - Microbiology 2021Quote: ... and proteins were purified with Ni-NTA resin (Qiagen, Cat# 151010181) and followed by Superose 6 10/300 gel filtration column chromatography (GE Healthcare ...
-
bioRxiv - Biophysics 2020Quote: ... The solubilized protein was then purified using Ni-NTA agarose (Qiagen). The purity was confirmed (>98% ...
-
bioRxiv - Microbiology 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen), the eluted fractions containing S protein were pooled ...
-
bioRxiv - Microbiology 2021Quote: ... cruzi proteins attached to addressable Liquichip Ni-NTA beads (Qiagen, CA) and T ...
-
bioRxiv - Molecular Biology 2021Quote: ... The fusion protein was affinity purified using Ni-TNA agarose (Qiagen). Details are provided in the Supplementary Information methods section.
-
bioRxiv - Microbiology 2022Quote: ... Protein was purified under native conditions according to the QIAexpressionist (Qiagen) protocol ...
-
bioRxiv - Molecular Biology 2022Quote: The His-tagged protein was purified by Ni-NTA agarose (Qiagen). Eluted proteins from beads were then applied to the NGC chromatography system (Bio–Rad) ...
-
bioRxiv - Microbiology 2023Quote: ... Protein was purified from sonicated lysates using Ni-NTA resin (Qiagen) and gravity flow ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Novex, Qiagen) using MOPS running buffer (Novex ...
-
bioRxiv - Developmental Biology 2023Quote: ... After the protein digestion with Proteinase K (QIAGEN, Cat. No. #158920) and RNA digestion with RNase A (QIAGEN ...
-
bioRxiv - Cell Biology 2023Quote: ... and AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen). Isolated DNA and RNA samples were quantified by Nanodrop (ThermoFischer Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen) and a gravity column (Biorad) ...
-
bioRxiv - Biochemistry 2023Quote: ... The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
-
bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
-
bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
-
bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen), eluted with 50 mM Tris-base ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). To purify JetAB complexes containing one full-length and one truncated JetA protomer ...
-
bioRxiv - Microbiology 2019Quote: ... and HRP-penta-His antibody (Qiagen) and HRP-anti-mouse IgG (Abcam ...
-
bioRxiv - Biochemistry 2023Quote: ... The PentaHis antibody (Qiagen, Düsseldorf, DE) against His-Tagged proteins was diluted 1:5000 in TBS buffer containing 3% BSA and the membrane was incubated overnight at 4°C followed by 2×10 min washing steps in TBST and 1×10 min in TBS buffers ...
-
bioRxiv - Biophysics 2023Quote: ... anti-Strep-tag antibody (Qiagen, 34850), or anti-HA-tag antibody (Cell Signaling ...
-
bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
-
bioRxiv - Cell Biology 2019Quote: ... 20ng of total DNA was used in a qPCR reaction containing QuantiNova SYBR Green mix (Qiagen), which was ran on a StepOnePlus Real-Time platform (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... The plasmid containing the cloned chitinase gene was isolated using the Qiaprep spin miniprep kit (Qiagen). Restriction digestion of the plasmid was done with XhoI and XmaI ...
-
bioRxiv - Molecular Biology 2021Quote: ... the upper aqueous phase containing RNA was automatically purified with an RNeasy Plus Mini Kit (QIAGEN) on a QIAcube instrument (QIAGEN) ...
-
bioRxiv - Neuroscience 2019Quote: All plasmids containing reporter transgenes were prepared using Qiagen Plasmid Mini Kit (Qiagen Ref No.12125). The plasmids were sent to Genetivision Corporation for injection ...
-
bioRxiv - Genetics 2019Quote: ... for lysis in 2 ml safe-lock tubes containing one 5 mm stainless steel bead (Qiagen) for 2.5-3 minutes at 30 Hz using TissueLyzer II (Qiagen) ...