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Citations for Qiagen :
251 - 300 of 1315 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... After incubation with the primary antibody from QIAGEN (Penta-His Antibody, 1:1000 dilution) at RT for 1 h under shaking ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Lysates were incubated for 1 hr at 37°C after adding 10 µl of RNAse A (20 mg/ml) and proteins were precipitated with 200 µl of Protein Precipitation Solution (Qiagen Gentra Puregen Cell kit). After centrifugation ...
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bioRxiv - Genetics 2022Quote: ... using biotinylated His5-antibody (Qiagen), as previously described (Asbury et al. ...
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bioRxiv - Cell Biology 2022Quote: ... single cells were sorted into PCR plates containing 5 µl Buffer RLT Plus (Qiagen) with 1% BME and immediately frozen at -80°C for G&T sequencing.
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bioRxiv - Microbiology 2020Quote: ... Samples were placed separately in labelled tubes containing 200 µL ATL buffer (Qiagen, USA) and stored under refrigeration until transported to the lab for storage at -20°C ...
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bioRxiv - Microbiology 2020Quote: ... a total cold volume of 7 μl containing 300 ng random hexamers (Qiagen Operon), 12 U RNasin (Promega ...
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bioRxiv - Immunology 2022Quote: ... The tissue was transferred to 600 μl of RLT buffer containing β-mercaptoethanol (Qiagen) and homogenized for 30 sec with a hand held rotor (Tissue-Tearor ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was applied to a fritted column containing Ni-NTA resin (Qiagen, USA) which had been pre-equilibrated with several column volumes of lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... in a volume of 25 μl containing 12.5 μl PCR buffer (HotstarTaq mastermix; Qiagen), 0.5 μl MgCl2 (25mM) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The supernatant was applied to a fritted column containing Ni-NTA resin (Qiagen, USA) which had been pre-equilibrated with several column volumes of lysis buffer ...
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bioRxiv - Synthetic Biology 2019Quote: The supernatant was applied to a fritted column containing Ni-NTA resin (Qiagen, USA) and the proteins were purified using the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... immune and non-immune single cells into 96-well plates containing TCL buffer (Qiagen) with 1% beta-mercaptoethanol ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was applied to a fritted column containing Ni-NTA resin (Qiagen, USA) which had been pre-equilibrated with several column volumes of lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: pBABE derivatives containing empty IRES_EGFP or different Cdc20_IRES2_EGFP constructs were transfected with Effectene (Qiagen) along with VSVG packaging plasmid into 293-GP cells for generation of retrovirus as described 29 ...
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bioRxiv - Immunology 2021Quote: ... Each tissue was collected into a microcentrifuge tube containing 1 mL of Qiazol (Qiagen) and autoclaved glass beads ...
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bioRxiv - Immunology 2021Quote: ... The clear solution containing lysate was passed through the RNeasy mini column (Qiagen, Germany), which leads to the binding of RNA with the column ...
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bioRxiv - Developmental Biology 2022Quote: ... This RNA-containing solution was processed using the RNeasy® Mini Kit (QIAGEN, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Individual flies were placed in tubes containing 1.4 mm ceramic beads (Qiagen; MD, USA) and ATL buffer from PowerMag Bead Solution (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were loaded onto a gravity-flow column containing Ni–NTA resin (Qiagen) equilibrated in lysis buffer (without PMSF) ...
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bioRxiv - Cell Biology 2023Quote: ... flow sorted into 96 well plates containing 2.5ul of RLT plus lysis buffer (Qiagen), collecting only viable singlets ...
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bioRxiv - Microbiology 2023Quote: Lungs were collected in 500 µL of DPBS containing one stainless steel bead (QIAGEN), homogenized with the Tissue-Lyser II (QIAGEN) ...
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bioRxiv - Immunology 2023Quote: ... ∼1000 macrophages were sorted into 75µL of RLT buffer (Qiagen, containing 1% beta-mercaptoethanol), vortexed for 1 min and immediately frozen (–80°C) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were sorted into 384 well plates each containing 3 μl Vapor-Lock (Qiagen) in all wells and eight different conditions of 0.3 μl lysis buffer consisting of 0.125μM OligodT30VN (5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... The top aqueous layer containing RNA was transferred to a QIAshredder column (Qiagen, #79656), and flow-through as mixed 1:1 with 70% ethanol ...
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bioRxiv - Immunology 2024Quote: ... a cold volume of 7 µL containing 300 ng of random hexamers (Qiagen Operon), 12 U Rnasin (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was purified using Ni-NTA Fast Start (Qiagen, #30600) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... refolded protein mixture was loaded on Ni-NTA beads (Qiagen) as 1ml per 2 liter culture ...
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bioRxiv - Developmental Biology 2020Quote: ... recombinant proteins were purified using Ni-NTA Agarose (Qiagen 30210). Buffer was exchanged by dialysis to PBS ...
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bioRxiv - Microbiology 2019Quote: ... His-tagged proteins were purified over Ni-NTA-agarose (Qiagen) with 3 wash steps at 30 mM imidazole and elution with 250 mM imidazole in denaturing buffer ...
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bioRxiv - Neuroscience 2019Quote: ... for protein extraction or 200 µL of RLT buffer (Qiagen) for RNA extraction and stored at −80°C until further sample processing.
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bioRxiv - Biochemistry 2019Quote: ... Proteins were purified by Ni2+-affinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was affinity-purified using Ni-NTA agarose (Qiagen #301210), and exchanged into severing buffer I (SBI ...
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bioRxiv - Biochemistry 2021Quote: ... Protein crystallization was screened for in commercially available screens (Qiagen), using a Crystal Gryphon robot (Art Robbins Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified using Ni-NTA affinity columns (Qiagen) and subsequently purified by gel filtration using a HiLoad Superdex 75 pg column (GE) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then purified using Ni-NTA agarose (Qiagen 30210). The amount of purified protein was estimated both by conducting BCA assays (Thermo Fisher 23225 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (QIAGEN) and were eluted with 300 mM imidazole in PBS buffer ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... RNA was extracted using the Allprep RNA/Protein Kit (Qiagen). The quality and quantity of RNA were assessed using the Experion RNA Analysis Kit (BioRad ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Microbiology 2022Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Immunology 2019Quote: ... Proteins were purified by affinity chromatography on NiNTA agarose (Qiagen), followed by FPLC on MonoQ (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni-NTA affinity chromatography (Qiagen) and eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: Protein purification was done using Ni-NTA Spin Columns (Qiagen) following the supplier recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... or the AllPrep DNA/RNA/Protein mini kit (Qiagen #80004) and quantified using 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (Qiagen) and eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-NTA agarose resin (Qiagen), washed with 20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... and His6-tagged protein was captured on NiNTA agarose (Qiagen). Following extensive washes with core buffer supplemented with 20 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were affinity purified on Ni-NTA agarose beads (Qiagen). Proteins eluted from Ni-NTA beads by stepwise addition of increasing concentrations of 50-300 mM imidazole in a buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... and the resulting proteins were purified using Ni-resins (Qiagen) and size exclusion chromatography (HiLoad Superdex 75 ...
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bioRxiv - Immunology 2021Quote: ... and the resulting proteins were purified using Ni-resins (Qiagen). The vector encoding a hexa-His tag fused to a single chain homotrimeric mLIGHT extracellular domain (G73-V239 ...