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Citations for Qiagen :
351 - 400 of 3538 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer from the RNAeasy Plus Micro kit (Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... The incubation period at 56°C was set to one hour for all samples and all samples were treated with 4 µl RNase A (100 mg/ml, Qiagen). DNA was eluted in 100 µl TE buffer with 0.1 mM EDTA ...
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bioRxiv - Cancer Biology 2022Quote: ... Library plasmid pools were propagated in liquid culture in LB with ampicillin (100 μg/ml) at 30 °C overnight and extracted (Qiagen).
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bioRxiv - Microbiology 2019Quote: U1 cells were treated with 100 μg/ml concentration of exosomes for 12 h and total RNA was isolated using RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer from the RNAeasy Plus Micro kit (Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Then 4 μl RNase A (100 mg/ml) were added to the tubes followed by 150 μl of buffer AL (lysis buffer, Qiagen). After mixing by vortexing ...
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 30°C overnight in presence of a selective antibiotic Ampicillin (100 µg/ml) and isolated the final plasmid via a Midiprep using a Qiafilater plasmid midi kit (Qiagen) the following day.
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bioRxiv - Immunology 2023Quote: ... Plasmid DNA from overnight liquid cultures (LB with 100 mg/ mL ampicillin) was isolated with QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Genomics 2023Quote: ... After ligation nuclei were pelleted and resuspended in cold PBS with DAPI to a final concentration of 300nM and GFP+ cells were FAC-sorted into a 96 well plate with 2ul lysis buffer (20mM Tris pH 8, 20mM NaCl, 0.15% Triton X-100, 25mM DTT, 1mM EDTA, 500nM Carrier ssDNA, and 15ug/mL Qiagen Protease) and lysed for 1 hour at 50°C and inactivated 15 minutes at 70°C ...
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bioRxiv - Genomics 2023Quote: ... then stopped with transposome removal buffer (300nM NaCl, 45 mM EDTA, 0.01% Triton X-100 with 100ug/mL Qiagen Protease) and incubated at 50°C for 40 minutes and 70°C for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 U/mL RNasin) and resuspended in 100 µL of RLT buffer from the RNeasy Micro Kit (Qiagen 74004) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer (RNAeasy Plus Micro kit, Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Immunology 2020Quote: ... were homogenised in a Precellys 24 homogeniser with CK28 Hard tissue homogenizing 2.0 ml tubes (Bertin) and 1 ml of RLT buffer (Qiagen) supplemented with 1%(v/v ...
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bioRxiv - Systems Biology 2023Quote: ... A culture volume equal to 3 mL of OD = 1 was added to 6 mL RNAprotect Bacteria Reagent (Qiagen), vortexed ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from 1-2 million cells using RNeasy Mini Kit (QIAGEN) using QIAcube (QIAGEN) ...
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bioRxiv - Paleontology 2019Quote: ... plasmid minipreps were purified with a QIAprep Miniprep Kit (Qiagen, chimpanzee 1 and 2), and RBC Miniprep Kit (YPD100 ...
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bioRxiv - Biophysics 2021Quote: cfDNA was isolated from 2 mL of plasma for each sample via the QIAamp Circulating Nucleic Acid kit (Qiagen). ddPCR was performed using the QX200 Droplet Digital PCR System (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... each individual cell was transferred to a 0.2 ml PCR tube containing 2 μl of lysis buffer (RLT Plus - Qiagen). The tube was immediately placed on a metal plate sitting on top of dry ice for flash-freezing ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein was normalized to 2 mg across all samples tested and incubated with 0.25 mL of 50% Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of logarithmic growth phase culture was pelleted by centrifugation and resuspended by vortexing in B1 buffer (Qiagen, Hilden Germany ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 2 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from 2 mL of each of the nine overnight cultures (DNeasy Blood & Tissue Kit; Qiagen). Library preparation and sequencing was performed by Novogene Europe (Cambridge ...
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bioRxiv - Immunology 2019Quote: ... mucosal scrapings and feces were homogenized in a 2 ml eppendorf containing 1 ml of DNA extraction buffer and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Neuroscience 2021Quote: ... and Halt Protease inhibitor (Thermo-Scientific) (1:100) for 1min at 30Hz using the TissuelyserLT (Qiagen). Homogenates were transferred to chilled Eppendorf tubes (−20°C ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNAs were prepared in RLT RNA lysis buffer supplemented with 1:100 β-mercaptoethanol (Qiagen) at 5,000 cells/µl ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria was split across six tubes and each recovered in 2 mL of SOC for 1 h at 37 °C then added to 500 mL of LB with 100 μg/mL of carbenicillin and grown for 9 h at 37 °C prior to plasmid purification (Qiagen, 12991). The plasmid prep was then normalized to 1 μg/μL to generate the final mpra:minP:gfp library used for transfection delivery.
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately half the volume of each feces sample (100-200 mg) was immersed in 3 ml of Inhibit EX Buffer (QIAGEN, Hilden) and stored at room temperature ...
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bioRxiv - Microbiology 2022Quote: Microbial DNA was extracted from 0.5 ml of mucilage and 100 mg of epicuticular wax using a DNeasy PowerSoil kit (Qiagen, Maryland, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and 20 U/ml RNasin just before use) and resuspended in 100 μl RLT Buffer from the RNeasy Micro Kit (Qiagen #74004) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2023Quote: ... and 100 µg/mL streptomycin and were transfected with 800 ng of pAc5-3xFLAG-HRJDs using Effectene Transfection Reagent (QIAGEN, 301427) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and 100 µg/mL streptomycin and were transfected with 200 ng of pAc5-3xFLAG-HRJDs using Effectene Transfection Reagent (QIAGEN, 301427) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Spheroids were transferred into PCR-clean 1.5 mL microtubes with 100 µL of lysis buffer (LB1 from Macherey-Nagel or 700 µL of Buffer RLT (79216, Qiagen). Lysates were snap-frozen and stored at −80 °C ...
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bioRxiv - Genomics 2023Quote: ... and grown O/N in 4L LB-Amp (Luria-Bertani medium plus ampicillin, 100 µg/ml) and purified using Plasmid Plus Giga Kit (Qiagen, #12991). Finally ...
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bioRxiv - Genomics 2024Quote: ... Batches of approximately 20 individuals were transferred to a 1.5-mL microcentrifuge tube with 100 µL of G2 Buffer (lysis buffer from Genomic DNA Buffer Set, Qiagen ID: 19060) and carefully crushed with a plastic pestle less than four hours after their collection.
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bioRxiv - Microbiology 2020Quote: ... and the pellet was immediately resuspended in 1 mL RNA-Protect (Qiagen). RNA-stabilized cell pellets were stored at −80 °C.
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bioRxiv - Microbiology 2020Quote: ... 1 mL was centrifuged and washed twice with nuclease-free water (Qiagen). The cell pellet was resuspended in 500 µL of nuclease-free water ...
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bioRxiv - Cell Biology 2020Quote: ... was carried out after treatment with 50 μg ml−1 RNase (Qiagen) for 20 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... the supernatant was loaded onto a 1 ml Strep-Tactin column (Qiagen) at a flow rate of 0.7 ml/min in the ÄKTA prime-plus liquid chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified lysate was incubated with 1 ml Ni-NTA resin (Qiagen) for 1 h ...
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bioRxiv - Biochemistry 2022Quote: Aliquots (1 ml) of culture were added to RNAProtect Bacteria Reagent (Qiagen) (2 ml) ...
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bioRxiv - Cell Biology 2019Quote: ... Two hundred microliters of 1-mg/ml RNase A (Qiagen, Germantown, MD) was added ...
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bioRxiv - Microbiology 2020Quote: ... harvested pellets were resuspended in 1 ml RNA Protect Bacteria Reagent (Qiagen), incubated for 5 min at ambient temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM imidazole (final concentration) and 1 ml Ni-NTA resin (Qiagen) pre-washed in 25mM Hepes ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was incubated with 1 mL of Ni- NTA agarose (Qiagen) for 1 h at 25 °C with rotating ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was mixed with 1 ml Ni-NTA-Agarose slurry (Qiagen) in an overhead rotator (10 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were homogenised in 1 ml QIAzol lysis reagent (Qiagen, Hilden, Germany) with a handheld tissue homogeniser (Appleton Woods Ltd ...